Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 150396297

GI number: 150396297

Start: 1145546

End: 1146943

Strand: Direct

Name: pdhB [H]

Synonym: Smed_1077

Alternate gene names: 150396297

Gene position: 1145546-1146943 (Clockwise)

Preceding gene: 150396296

Following gene: 150396298

Centisome position: 30.29

GC content: 62.16

Gene sequence:

>1398_bases
ATGCCTGTAGAAATCCTGATGCCTGCCCTTTCCCCGACCATGGAGGAAGGCACGCTCTCCAAGTGGCTGAAGAATGAGGG
AGACAAGGTCTCCTCCGGCGACGTCATCGCCGAAATCGAAACCGACAAGGCGACCATGGAAGTGGAAGCCGTTGACGAGG
GGACGATCGGCAAACTGCTGATCGCGGCCGGCACCGAAGGCGTGAAGGTGAACACGCCGATCGCCGTATTGCTGCAGGAC
GGCGAAGCCGCAAGCGACATCGATACCGCGAAGGCCGAGGCGCCGAAAGCGGAAGCTCCGAAAGCCGAAGCGCCGAAGCA
GGGCGATCCTGAAGCGCCTGCCGCTTCTGCAGCGCCCGTTGCGGCGCAGCCCAGGGCGGACGTTCCTTCCGATCCGGCAA
TTCCGGCCGGAACCGAGATGGTGACGATGACCGTCCGCGAAGCGCTTCGCGATGCGATGGCCGAAGAAATGCGCGCCAAT
GACGATGTCTTCGTCATGGGCGAAGAGGTCGCCGAATACCAGGGCGCCTACAAGATCACGCAGGGCCTTTTGCAGGAATT
CGGAGCCCGCCGCGTCGTCGACACGCCGATTACGGAACATGGCTTTGCCGGCGTCGGCGTCGGTGCTGCGATGACGGGTC
TGCGCCCGATCGTCGAATTCATGACCTTCAACTTCGCCATGCAGGCGATTGATCAGATCATCAATTCGGCCGCAAAGACG
CTGTATATGTCCGGCGGCCAGATGGGTGCACCGATCGTCTTCCGCGGCCCGAGCGGCGCTGCCGCGCGCGTCGCCGCGCA
GCACTCGCAGTGCTACGCCGCCTGGTATAGCCATATTCCGGGGCTGAAGGTGGTGATGCCCTATACGGCCGCGGATGCCA
AGGGCCTGCTCAAGGCCGCGATCCGGGATCCGAATCCGATTATCTTCCTCGAAAACGAAATCCTTTACGGTCAGTCCTTC
GATGTTCCGAAGCTCGACGATTTCGTGCTGCCGATCGGCAAGGCCCGCATCCATCGCGCCGGCAAGGACGCGACGCTCGT
CTCCTTCGGCATCGGCATGACCTATGCGATCAAGGCCGCGGCGGAACTGGAGGCGCAGGGGATCGATGTGGAGATCATCG
ATCTTCGCACCATCCGTCCGATGGACCTGCCGACCGTCATCGAATCGGTCAAGAAGACCGGCCGCCTCGTCACGGTCGAA
GAGGGCTATCCGCAGTCTTCCGTCGGCACCGAAATCGCAACCCGGGTGATGCAGCAGGCCTTCGACTATCTCGACGCACC
GGTGCTGACGATCGCCGGCAAGGACGTGCCGATGCCCTATGCGGCCAATCTTGAAAAGCTCGCATTGCCGAGCGTCGCCG
AAGTCGTCGAGGCGGTGAAAGCCGTCTGCTACAAATAA

Upstream 100 bases:

>100_bases
ACATCGTCGCGGACAGTGCCGATTTCGCCCAGTCTGATCCGGAGCCGGATGTTTCCGAGCTCTACACCGATATCCTGCTT
TGATCCGGGGAGGGATAAAT

Downstream 100 bases:

>100_bases
AAGGGGGCCGTTCGATGCCAATCAACATCACCATGCCGGCCCTCTCTCCGACGATGGAAGAAGGCAATCTCGCCAAGTGG
CTGGTCAAGGAAGGCGATAA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPVEILMPALSPTMEEGTLSKWLKNEGDKVSSGDVIAEIETDKATMEVEAVDEGTIGKLLIAAGTEGVKVNTPIAVLLQD
GEAASDIDTAKAEAPKAEAPKAEAPKQGDPEAPAASAAPVAAQPRADVPSDPAIPAGTEMVTMTVREALRDAMAEEMRAN
DDVFVMGEEVAEYQGAYKITQGLLQEFGARRVVDTPITEHGFAGVGVGAAMTGLRPIVEFMTFNFAMQAIDQIINSAAKT
LYMSGGQMGAPIVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPIIFLENEILYGQSF
DVPKLDDFVLPIGKARIHRAGKDATLVSFGIGMTYAIKAAAELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVTVE
EGYPQSSVGTEIATRVMQQAFDYLDAPVLTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVCYK

Sequences:

>Translated_465_residues
MPVEILMPALSPTMEEGTLSKWLKNEGDKVSSGDVIAEIETDKATMEVEAVDEGTIGKLLIAAGTEGVKVNTPIAVLLQD
GEAASDIDTAKAEAPKAEAPKAEAPKQGDPEAPAASAAPVAAQPRADVPSDPAIPAGTEMVTMTVREALRDAMAEEMRAN
DDVFVMGEEVAEYQGAYKITQGLLQEFGARRVVDTPITEHGFAGVGVGAAMTGLRPIVEFMTFNFAMQAIDQIINSAAKT
LYMSGGQMGAPIVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPIIFLENEILYGQSF
DVPKLDDFVLPIGKARIHRAGKDATLVSFGIGMTYAIKAAAELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVTVE
EGYPQSSVGTEIATRVMQQAFDYLDAPVLTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVCYK
>Mature_464_residues
PVEILMPALSPTMEEGTLSKWLKNEGDKVSSGDVIAEIETDKATMEVEAVDEGTIGKLLIAAGTEGVKVNTPIAVLLQDG
EAASDIDTAKAEAPKAEAPKAEAPKQGDPEAPAASAAPVAAQPRADVPSDPAIPAGTEMVTMTVREALRDAMAEEMRAND
DVFVMGEEVAEYQGAYKITQGLLQEFGARRVVDTPITEHGFAGVGVGAAMTGLRPIVEFMTFNFAMQAIDQIINSAAKTL
YMSGGQMGAPIVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPIIFLENEILYGQSFD
VPKLDDFVLPIGKARIHRAGKDATLVSFGIGMTYAIKAAAELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVTVEE
GYPQSSVGTEIATRVMQQAFDYLDAPVLTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVCYK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=56.4814814814815, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=53.0864197530864, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI4557353, Length=321, Percent_Identity=34.2679127725857, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI34101272, Length=321, Percent_Identity=34.2679127725857, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI203098753, Length=99, Percent_Identity=43.4343434343434, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI203098816, Length=94, Percent_Identity=42.5531914893617, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI31711992, Length=140, Percent_Identity=35.7142857142857, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=57.6271186440678, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI225637463, Length=330, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI225637459, Length=290, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI225637461, Length=290, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI205277463, Length=306, Percent_Identity=25.1633986928105, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI4507521, Length=306, Percent_Identity=25.1633986928105, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI133778974, Length=322, Percent_Identity=25.776397515528, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=63.1578947368421, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17506935, Length=333, Percent_Identity=40.5405405405405, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17560088, Length=137, Percent_Identity=40.1459854014599, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=57.1865443425077, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324258, Length=137, Percent_Identity=40.1459854014599, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=120, Percent_Identity=35.8333333333333, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21358145, Length=320, Percent_Identity=60.3125, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24650940, Length=320, Percent_Identity=60.3125, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=35.2941176470588, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=35.2941176470588, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=62.2222222222222, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=62.2222222222222, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI20129315, Length=78, Percent_Identity=47.4358974358974, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551847, Length=312, Percent_Identity=27.2435897435897, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550715, Length=312, Percent_Identity=27.2435897435897, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24645119, Length=312, Percent_Identity=27.2435897435897, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582497, Length=67, Percent_Identity=46.2686567164179, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49289; Mature: 49158

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVEILMPALSPTMEEGTLSKWLKNEGDKVSSGDVIAEIETDKATMEVEAVDEGTIGKLL
CCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEE
IAAGTEGVKVNTPIAVLLQDGEAASDIDTAKAEAPKAEAPKAEAPKQGDPEAPAASAAPV
EEECCCCEEECCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAQPRADVPSDPAIPAGTEMVTMTVREALRDAMAEEMRANDDVFVMGEEVAEYQGAYKIT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHH
QGLLQEFGARRVVDTPITEHGFAGVGVGAAMTGLRPIVEFMTFNFAMQAIDQIINSAAKT
HHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LYMSGGQMGAPIVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAA
EEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
IRDPNPIIFLENEILYGQSFDVPKLDDFVLPIGKARIHRAGKDATLVSFGIGMTYAIKAA
HCCCCCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHHCCCCCEEEEECCCHHHHHHHH
AELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYPQSSVGTEIATRVMQQA
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
FDYLDAPVLTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVCYK
HHHHCCCEEEEECCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVEILMPALSPTMEEGTLSKWLKNEGDKVSSGDVIAEIETDKATMEVEAVDEGTIGKLL
CCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEE
IAAGTEGVKVNTPIAVLLQDGEAASDIDTAKAEAPKAEAPKAEAPKQGDPEAPAASAAPV
EEECCCCEEECCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAQPRADVPSDPAIPAGTEMVTMTVREALRDAMAEEMRANDDVFVMGEEVAEYQGAYKIT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHH
QGLLQEFGARRVVDTPITEHGFAGVGVGAAMTGLRPIVEFMTFNFAMQAIDQIINSAAKT
HHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LYMSGGQMGAPIVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAA
EEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
IRDPNPIIFLENEILYGQSFDVPKLDDFVLPIGKARIHRAGKDATLVSFGIGMTYAIKAA
HCCCCCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHHCCCCCEEEEECCCHHHHHHHH
AELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYPQSSVGTEIATRVMQQA
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
FDYLDAPVLTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVCYK
HHHHCCCEEEEECCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]