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The map label for this gene is pdhA [H]

Identifier: 150396296

GI number: 150396296

Start: 1144482

End: 1145528

Strand: Direct

Name: pdhA [H]

Synonym: Smed_1076

Alternate gene names: 150396296

Gene position: 1144482-1145528 (Clockwise)

Preceding gene: 150396295

Following gene: 150396297

Centisome position: 30.26

GC content: 60.27

Gene sequence:

>1047_bases
ATGGCTCCGCGAAAAACCGCGTCCGTTTCCAGCCGCAAAACCGCTGCCAAGCCGGTCAAGAAAGATTTTGCCGGCGGCAC
GATCGCCGAGTTCTCCAAGGAAGACGATCTCAAGGCTTACCGCGAAATGCTGTTGATCCGGCGTTTCGAGGAAAAGGCCG
GCCAGCTTTATGGCATGGGGTTCATCGGCGGTTTCTGTCACCTCTATATCGGCCAGGAAGCCGTCGTCGTCGGCATGCAG
CTTGCCTTGAAAGAGGGCGATCAGGTCATCACCGGCTATCGTGACCACGGCCACATGCTCGCCTGCGGCATGAGTGCGCG
CGGCGTGATGGCGGAGCTCACCGGTCGCCGTGGCGGCCTTTCCAAGGGGAAGGGCGGCTCGATGCATATGTTCTCCAAGG
AAAAACACTTCTATGGCGGTCACGGCATCGTCGGTGCGCAGGTTTCGCTCGGCACCGGCCTTGCCTTCGCCAACAGATAT
CGCGGCAACGACAATGTCAGCCTCGCCTATTTCGGCGACGGTGCGGCCAATCAGGGCCAGGTCTATGAGAGCTTCAACAT
GGCCGCTCTCTGGAAATTGCCGGTGATCTACATCGTCGAAAACAACCGCTATGCCATGGGTACCTCCGTGTCGCGTGCCT
CGGCGCAGACCGACTTCTCCCAGCGCGGCGCATCCTTCGGCATTCCGGGCTATCAGGTGGACGGCATGGATGTCCGCGCC
GTCAAGGCCGCAGCCGACGAGGCGGTGGAGCATTGCCGTTCCGGCAAGGGGCCGATCATCCTTGAGATGCTGACCTACCG
CTACCGCGGCCATTCGATGTCCGATCCGGCGAAGTATCGCTCGAAGGACGAAGTACAGAAGATGCGTTCGGAGCATGATC
CGATCGAGCAGGTGAAGGCCCGCCTCATGGATAAAGGCTGGGCCACCGAGGATGAGCTGAAGCAGATCGACAAGGAGGTT
CGCGACATCGTCGCGGACAGTGCCGATTTCGCCCAGTCTGATCCGGAGCCGGATGTTTCCGAGCTCTACACCGATATCCT
GCTTTGA

Upstream 100 bases:

>100_bases
CAGTGGCTTGGTGCGAATAATAGCCATGCAAATATGGCATTGCTGCCGCGCTTTTCTTTCCCTATGGTAGCGATCAAAGG
CTAACCATTGGGAGGAACGA

Downstream 100 bases:

>100_bases
TCCGGGGAGGGATAAATATGCCTGTAGAAATCCTGATGCCTGCCCTTTCCCCGACCATGGAGGAAGGCACGCTCTCCAAG
TGGCTGAAGAATGAGGGAGA

Product: dehydrogenase E1 component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MAPRKTASVSSRKTAAKPVKKDFAGGTIAEFSKEDDLKAYREMLLIRRFEEKAGQLYGMGFIGGFCHLYIGQEAVVVGMQ
LALKEGDQVITGYRDHGHMLACGMSARGVMAELTGRRGGLSKGKGGSMHMFSKEKHFYGGHGIVGAQVSLGTGLAFANRY
RGNDNVSLAYFGDGAANQGQVYESFNMAALWKLPVIYIVENNRYAMGTSVSRASAQTDFSQRGASFGIPGYQVDGMDVRA
VKAAADEAVEHCRSGKGPIILEMLTYRYRGHSMSDPAKYRSKDEVQKMRSEHDPIEQVKARLMDKGWATEDELKQIDKEV
RDIVADSADFAQSDPEPDVSELYTDILL

Sequences:

>Translated_348_residues
MAPRKTASVSSRKTAAKPVKKDFAGGTIAEFSKEDDLKAYREMLLIRRFEEKAGQLYGMGFIGGFCHLYIGQEAVVVGMQ
LALKEGDQVITGYRDHGHMLACGMSARGVMAELTGRRGGLSKGKGGSMHMFSKEKHFYGGHGIVGAQVSLGTGLAFANRY
RGNDNVSLAYFGDGAANQGQVYESFNMAALWKLPVIYIVENNRYAMGTSVSRASAQTDFSQRGASFGIPGYQVDGMDVRA
VKAAADEAVEHCRSGKGPIILEMLTYRYRGHSMSDPAKYRSKDEVQKMRSEHDPIEQVKARLMDKGWATEDELKQIDKEV
RDIVADSADFAQSDPEPDVSELYTDILL
>Mature_347_residues
APRKTASVSSRKTAAKPVKKDFAGGTIAEFSKEDDLKAYREMLLIRRFEEKAGQLYGMGFIGGFCHLYIGQEAVVVGMQL
ALKEGDQVITGYRDHGHMLACGMSARGVMAELTGRRGGLSKGKGGSMHMFSKEKHFYGGHGIVGAQVSLGTGLAFANRYR
GNDNVSLAYFGDGAANQGQVYESFNMAALWKLPVIYIVENNRYAMGTSVSRASAQTDFSQRGASFGIPGYQVDGMDVRAV
KAAADEAVEHCRSGKGPIILEMLTYRYRGHSMSDPAKYRSKDEVQKMRSEHDPIEQVKARLMDKGWATEDELKQIDKEVR
DIVADSADFAQSDPEPDVSELYTDILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=313, Percent_Identity=55.591054313099, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI291084742, Length=313, Percent_Identity=56.2300319488818, Blast_Score=341, Evalue=5e-94,
Organism=Homo sapiens, GI4505685, Length=313, Percent_Identity=55.9105431309904, Blast_Score=341, Evalue=6e-94,
Organism=Homo sapiens, GI291084744, Length=320, Percent_Identity=54.6875, Blast_Score=334, Evalue=7e-92,
Organism=Homo sapiens, GI291084757, Length=313, Percent_Identity=50.1597444089457, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI11386135, Length=317, Percent_Identity=31.5457413249211, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI258645172, Length=317, Percent_Identity=31.8611987381703, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32564172, Length=342, Percent_Identity=50.8771929824561, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17536047, Length=342, Percent_Identity=50.8771929824561, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI86563355, Length=318, Percent_Identity=30.188679245283, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI86563357, Length=318, Percent_Identity=30.188679245283, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=51.2422360248447, Blast_Score=328, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24639744, Length=326, Percent_Identity=50.920245398773, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI28571106, Length=326, Percent_Identity=50.920245398773, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24639740, Length=321, Percent_Identity=51.4018691588785, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24639746, Length=305, Percent_Identity=52.4590163934426, Blast_Score=321, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24639748, Length=319, Percent_Identity=52.6645768025078, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21355903, Length=327, Percent_Identity=28.4403669724771, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017597 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 38186; Mature: 38055

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRKTASVSSRKTAAKPVKKDFAGGTIAEFSKEDDLKAYREMLLIRRFEEKAGQLYGMG
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECH
FIGGFCHLYIGQEAVVVGMQLALKEGDQVITGYRDHGHMLACGMSARGVMAELTGRRGGL
HHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHCCCCCCC
SKGKGGSMHMFSKEKHFYGGHGIVGAQVSLGTGLAFANRYRGNDNVSLAYFGDGAANQGQ
CCCCCCCEEEEECCCCEECCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCCC
VYESFNMAALWKLPVIYIVENNRYAMGTSVSRASAQTDFSQRGASFGIPGYQVDGMDVRA
HHHCCCCEEEEECCEEEEEECCEEEECCCCHHHHHHCCHHHCCCCCCCCCEEECCCCHHH
VKAAADEAVEHCRSGKGPIILEMLTYRYRGHSMSDPAKYRSKDEVQKMRSEHDPIEQVKA
HHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHH
RLMDKGWATEDELKQIDKEVRDIVADSADFAQSDPEPDVSELYTDILL
HHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
APRKTASVSSRKTAAKPVKKDFAGGTIAEFSKEDDLKAYREMLLIRRFEEKAGQLYGMG
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECH
FIGGFCHLYIGQEAVVVGMQLALKEGDQVITGYRDHGHMLACGMSARGVMAELTGRRGGL
HHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHCCCCCCC
SKGKGGSMHMFSKEKHFYGGHGIVGAQVSLGTGLAFANRYRGNDNVSLAYFGDGAANQGQ
CCCCCCCEEEEECCCCEECCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCCC
VYESFNMAALWKLPVIYIVENNRYAMGTSVSRASAQTDFSQRGASFGIPGYQVDGMDVRA
HHHCCCCEEEEECCEEEEEECCEEEECCCCHHHHHHCCHHHCCCCCCCCCEEECCCCHHH
VKAAADEAVEHCRSGKGPIILEMLTYRYRGHSMSDPAKYRSKDEVQKMRSEHDPIEQVKA
HHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHH
RLMDKGWATEDELKQIDKEVRDIVADSADFAQSDPEPDVSELYTDILL
HHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]