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The map label for this gene is pmbA [H]

Identifier: 150395646

GI number: 150395646

Start: 454976

End: 456322

Strand: Direct

Name: pmbA [H]

Synonym: Smed_0421

Alternate gene names: 150395646

Gene position: 454976-456322 (Clockwise)

Preceding gene: 150395643

Following gene: 150395647

Centisome position: 12.03

GC content: 64.81

Gene sequence:

>1347_bases
ATGTCTGAGACGATCGATTCCGCCGTCCTCGAGCAGCGCGCCGCCGAGCTTGTCGATCAGGCCCGCCGCGCTGGCGCGGA
TGCAGCAGATGCGGTGGTGGTGCGCGGGCGCTCGCGTTCCGTTTCCGTGCGGCTCGGCAAGGTGGAGGCGACCGAGGCTT
CCGAGAGCGACGATTTCTCGCTACGGGTCTTTGTCGGGCGCCGCGTCGCCAGCGTTTCCGCCAATCCCGGTTTCGATCTG
AAGCTCCTTGCGGAGCGTGCCGTGGCGATGGCGAAAGCATCGCCGGAGGATCCTTACGCATCTCTCGCCGATCCCGATCG
CCTGGCGAAGTCCTATCCGGATCTCGATCTTTTCGACAGCCGCGACGTATCCACCGAGACCCTCACCGAGAGGGCGCTCG
CCGCAGAGGAGGCCGCACTCTCGGTCACCGGCGTCACCAATTCGAGCGGGGCCGGTGCATCGGCCGGCATGGGCGGGCTC
GTGCTCGTCACGTCGCATGGTTTCTCCGGGTCCTATATGGCCACCCGTTTCGGCTGCTCCGTCAGCGCGATTGCCGGCGA
AGGCACCAAGATGGAGCGCGACTATGATTTCGACAGCCGTCTCTATTTCCACGAGCTCCGCACGGCCGAGGATATCGGCC
GCGTGGCGGGCGAGCGGGCGGCAGCGCGCATCAATCCCCGCCGCGTCGCGACGCAGAAGAACCTGACCGTCGTTTTCGAT
CCTCGCATCGCGCGCGGTTTCGTTGGCCATCTTGCAGGAGCCGTCAACGGTGCGTCGGTCGCCCGCAAGACGAGCTTCCT
GCGCGATATGATGGGCAAGCAGATCATGAAATCGGGTATTGCCGTAACCGACGATCCGTTGATCGTCCGCGGTGCCTCAT
CGCGTCCCTTCGACGGGGAGGGGGTCAGCGGCTCGAAACTGTCGATGGTCGAGGACGGTGTGCTGCGCCATTGGTTCCTG
TCCACGTCCGCAGCCAGGGAACTTGGCCTCGAAACCAACGGGCGGGGCGTTCGCGGTGGTCCGTCGGTCAGCCCGGCCTC
GACCAATCTCGCTCTCGAGCCCGGCTCAATTTCGCGCGAGGATCTGATCGGCGGCGTCGGCACGGGCTTCTATGTCACCG
AGCTCATCGGCCAGGGCGTCAACATGGTGACGGGCGAATACAGCCGCGGCGCTTCCGGCTTCTGGATCGAAAACGGCGAA
GTCCTCTATCCCGTGTCCGAGGTAACGATCGCCTCCAATCTGAAGCAGATGTTCATGGCCCTGACGCCTGCCGACGACAT
CGACAGGAAGTTCGGTATTGCCGCGCCGACCCTCGCGATCGAAGGCATGACGCTCGCCGGAACATGA

Upstream 100 bases:

>100_bases
TGGCCGAGTATCATTTTGCTGCTGGGACGGGAATCATGATGATGCCCTTGATGCATGCTTCAGTGCACAATAAATGGGAC
AGGATTGAAAGGTCAAAATC

Downstream 100 bases:

>100_bases
ATGTGGGCGGCGCGTTGCATGATGGGGATGAGGGAAGTGTGCGCGGATTTTCCGCCCGTGCCCCGCGTCTAAACTCTTTG
GAATCGATCGCGCTCATTTT

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSETIDSAVLEQRAAELVDQARRAGADAADAVVVRGRSRSVSVRLGKVEATEASESDDFSLRVFVGRRVASVSANPGFDL
KLLAERAVAMAKASPEDPYASLADPDRLAKSYPDLDLFDSRDVSTETLTERALAAEEAALSVTGVTNSSGAGASAGMGGL
VLVTSHGFSGSYMATRFGCSVSAIAGEGTKMERDYDFDSRLYFHELRTAEDIGRVAGERAAARINPRRVATQKNLTVVFD
PRIARGFVGHLAGAVNGASVARKTSFLRDMMGKQIMKSGIAVTDDPLIVRGASSRPFDGEGVSGSKLSMVEDGVLRHWFL
STSAARELGLETNGRGVRGGPSVSPASTNLALEPGSISREDLIGGVGTGFYVTELIGQGVNMVTGEYSRGASGFWIENGE
VLYPVSEVTIASNLKQMFMALTPADDIDRKFGIAAPTLAIEGMTLAGT

Sequences:

>Translated_448_residues
MSETIDSAVLEQRAAELVDQARRAGADAADAVVVRGRSRSVSVRLGKVEATEASESDDFSLRVFVGRRVASVSANPGFDL
KLLAERAVAMAKASPEDPYASLADPDRLAKSYPDLDLFDSRDVSTETLTERALAAEEAALSVTGVTNSSGAGASAGMGGL
VLVTSHGFSGSYMATRFGCSVSAIAGEGTKMERDYDFDSRLYFHELRTAEDIGRVAGERAAARINPRRVATQKNLTVVFD
PRIARGFVGHLAGAVNGASVARKTSFLRDMMGKQIMKSGIAVTDDPLIVRGASSRPFDGEGVSGSKLSMVEDGVLRHWFL
STSAARELGLETNGRGVRGGPSVSPASTNLALEPGSISREDLIGGVGTGFYVTELIGQGVNMVTGEYSRGASGFWIENGE
VLYPVSEVTIASNLKQMFMALTPADDIDRKFGIAAPTLAIEGMTLAGT
>Mature_447_residues
SETIDSAVLEQRAAELVDQARRAGADAADAVVVRGRSRSVSVRLGKVEATEASESDDFSLRVFVGRRVASVSANPGFDLK
LLAERAVAMAKASPEDPYASLADPDRLAKSYPDLDLFDSRDVSTETLTERALAAEEAALSVTGVTNSSGAGASAGMGGLV
LVTSHGFSGSYMATRFGCSVSAIAGEGTKMERDYDFDSRLYFHELRTAEDIGRVAGERAAARINPRRVATQKNLTVVFDP
RIARGFVGHLAGAVNGASVARKTSFLRDMMGKQIMKSGIAVTDDPLIVRGASSRPFDGEGVSGSKLSMVEDGVLRHWFLS
TSAARELGLETNGRGVRGGPSVSPASTNLALEPGSISREDLIGGVGTGFYVTELIGQGVNMVTGEYSRGASGFWIENGEV
LYPVSEVTIASNLKQMFMALTPADDIDRKFGIAAPTLAIEGMTLAGT

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=429, Percent_Identity=37.7622377622378, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1789640, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47150; Mature: 47018

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETIDSAVLEQRAAELVDQARRAGADAADAVVVRGRSRSVSVRLGKVEATEASESDDFS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEEEECCCCCCCCCE
LRVFVGRRVASVSANPGFDLKLLAERAVAMAKASPEDPYASLADPDRLAKSYPDLDLFDS
EEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
RDVSTETLTERALAAEEAALSVTGVTNSSGAGASAGMGGLVLVTSHGFSGSYMATRFGCS
CCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCE
VSAIAGEGTKMERDYDFDSRLYFHELRTAEDIGRVAGERAAARINPRRVATQKNLTVVFD
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEC
PRIARGFVGHLAGAVNGASVARKTSFLRDMMGKQIMKSGIAVTDDPLIVRGASSRPFDGE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCC
GVSGSKLSMVEDGVLRHWFLSTSAARELGLETNGRGVRGGPSVSPASTNLALEPGSISRE
CCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHH
DLIGGVGTGFYVTELIGQGVNMVTGEYSRGASGFWIENGEVLYPVSEVTIASNLKQMFMA
HHCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCEEECHHHHHHHHHHHHHHHH
LTPADDIDRKFGIAAPTLAIEGMTLAGT
CCCCHHHHHHCCCCCCCEEECCEEECCC
>Mature Secondary Structure 
SETIDSAVLEQRAAELVDQARRAGADAADAVVVRGRSRSVSVRLGKVEATEASESDDFS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEEEECCCCCCCCCE
LRVFVGRRVASVSANPGFDLKLLAERAVAMAKASPEDPYASLADPDRLAKSYPDLDLFDS
EEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
RDVSTETLTERALAAEEAALSVTGVTNSSGAGASAGMGGLVLVTSHGFSGSYMATRFGCS
CCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCE
VSAIAGEGTKMERDYDFDSRLYFHELRTAEDIGRVAGERAAARINPRRVATQKNLTVVFD
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEC
PRIARGFVGHLAGAVNGASVARKTSFLRDMMGKQIMKSGIAVTDDPLIVRGASSRPFDGE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCC
GVSGSKLSMVEDGVLRHWFLSTSAARELGLETNGRGVRGGPSVSPASTNLALEPGSISRE
CCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHH
DLIGGVGTGFYVTELIGQGVNMVTGEYSRGASGFWIENGEVLYPVSEVTIASNLKQMFMA
HHCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCEEECHHHHHHHHHHHHHHHH
LTPADDIDRKFGIAAPTLAIEGMTLAGT
CCCCHHHHHHCCCCCCCEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]