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The map label for this gene is yfcG [H]

Identifier: 150395631

GI number: 150395631

Start: 436224

End: 436931

Strand: Direct

Name: yfcG [H]

Synonym: Smed_0406

Alternate gene names: 150395631

Gene position: 436224-436931 (Clockwise)

Preceding gene: 150395630

Following gene: 150395632

Centisome position: 11.53

GC content: 59.18

Gene sequence:

>708_bases
ATGGCTGATCTTTCCTCCTTTCCGATCACGTCGCGCTGGCCGGCGAAAAATCCCGATATCATCCAGCTCTATTCGCTTCC
GACGCCGAACGGCGTGAAGGTCTCCATTGCTCTCGAAGAATTGGGCCTGACCTATGAGGCGCATCGCATTTCCTTCGATA
CCAACGAGCAGAAGTCGCCCGAATTCGTCTCCCTCAATCCGAACGGCCGCATTCCGGCGATCATCGATCCGAACGGGCCG
GACGGTAAGCCGATCGGCCTGTTCGAGTCGGGCGCGATCCTGATCTATCTCGCAGAAAAGACCGGCAAGCTCATTCCTGC
GGATGCGGCCCGCCGTTATGAGACGCTGTCCTGGGTGATGTTCCAGATGGGCGGCGTCGGACCAATGTTCGGGCAGTTCG
GCCATTTCTTCAAATTCGCGGCCGACAAGGTCGCGAACAATCCCTATCCCGTTCAGCGTTACCGAGACGAAGCAAAACGT
CTTCTCGGCGTTCTCGAAGCGAGGCTCGAAGGCAGGCAATGGCTGATGGGAGAGGAGTACACGATCGCCGATATCGCGAC
CTATCCCTGGGTGGAGGGGGCACGCAAATTCTATGGTGGAGCCGAGGTGCTCGACTATAAGAGCTTCCCGAAGGTCATGG
CATGGGTGGATCGCGGCCTGGCGCGCCCGGCCACGCAGAAGGGCATGGAGATCCCGCGCAAGGAGTGA

Upstream 100 bases:

>100_bases
AGGGCTCGCAAATTCGCCTTCGGCCCTGACAAACGCAACCGGCACTCCCTATGTTGGGGCGGCAGACACCCTTTCGATAT
CCCACCTAGAGGCATCCATT

Downstream 100 bases:

>100_bases
TTTGCAGCGGGAACCTTCCGGCCTGAGATGGACCGGCCACGGGCCGGGAGGATAGCGATCAACACGAACCTTTTGAGACG
GATGGAGCAGGTGTCGGGAA

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MADLSSFPITSRWPAKNPDIIQLYSLPTPNGVKVSIALEELGLTYEAHRISFDTNEQKSPEFVSLNPNGRIPAIIDPNGP
DGKPIGLFESGAILIYLAEKTGKLIPADAARRYETLSWVMFQMGGVGPMFGQFGHFFKFAADKVANNPYPVQRYRDEAKR
LLGVLEARLEGRQWLMGEEYTIADIATYPWVEGARKFYGGAEVLDYKSFPKVMAWVDRGLARPATQKGMEIPRKE

Sequences:

>Translated_235_residues
MADLSSFPITSRWPAKNPDIIQLYSLPTPNGVKVSIALEELGLTYEAHRISFDTNEQKSPEFVSLNPNGRIPAIIDPNGP
DGKPIGLFESGAILIYLAEKTGKLIPADAARRYETLSWVMFQMGGVGPMFGQFGHFFKFAADKVANNPYPVQRYRDEAKR
LLGVLEARLEGRQWLMGEEYTIADIATYPWVEGARKFYGGAEVLDYKSFPKVMAWVDRGLARPATQKGMEIPRKE
>Mature_234_residues
ADLSSFPITSRWPAKNPDIIQLYSLPTPNGVKVSIALEELGLTYEAHRISFDTNEQKSPEFVSLNPNGRIPAIIDPNGPD
GKPIGLFESGAILIYLAEKTGKLIPADAARRYETLSWVMFQMGGVGPMFGQFGHFFKFAADKVANNPYPVQRYRDEAKRL
LGVLEARLEGRQWLMGEEYTIADIATYPWVEGARKFYGGAEVLDYKSFPKVMAWVDRGLARPATQKGMEIPRKE

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=211, Percent_Identity=43.6018957345972, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI87082195, Length=221, Percent_Identity=42.5339366515837, Blast_Score=160, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324100, Length=239, Percent_Identity=34.7280334728033, Blast_Score=129, Evalue=4e-31,
Organism=Drosophila melanogaster, GI17933730, Length=201, Percent_Identity=29.3532338308458, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17864592, Length=206, Percent_Identity=28.6407766990291, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571670, Length=181, Percent_Identity=28.1767955801105, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922526, Length=210, Percent_Identity=28.0952380952381, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864598, Length=191, Percent_Identity=29.3193717277487, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26303; Mature: 26172

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLSSFPITSRWPAKNPDIIQLYSLPTPNGVKVSIALEELGLTYEAHRISFDTNEQKSP
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHCCEEEEEEEEECCCCCCCC
EFVSLNPNGRIPAIIDPNGPDGKPIGLFESGAILIYLAEKTGKLIPADAARRYETLSWVM
CEEEECCCCCCCEEECCCCCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
FQMGGVGPMFGQFGHFFKFAADKVANNPYPVQRYRDEAKRLLGVLEARLEGRQWLMGEEY
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCE
TIADIATYPWVEGARKFYGGAEVLDYKSFPKVMAWVDRGLARPATQKGMEIPRKE
EEEHHHHCCHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
ADLSSFPITSRWPAKNPDIIQLYSLPTPNGVKVSIALEELGLTYEAHRISFDTNEQKSP
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHCCEEEEEEEEECCCCCCCC
EFVSLNPNGRIPAIIDPNGPDGKPIGLFESGAILIYLAEKTGKLIPADAARRYETLSWVM
CEEEECCCCCCCEEECCCCCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
FQMGGVGPMFGQFGHFFKFAADKVANNPYPVQRYRDEAKRLLGVLEARLEGRQWLMGEEY
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCE
TIADIATYPWVEGARKFYGGAEVLDYKSFPKVMAWVDRGLARPATQKGMEIPRKE
EEEHHHHCCHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]