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The map label for this gene is thcD [H]

Identifier: 150395632

GI number: 150395632

Start: 436959

End: 438239

Strand: Direct

Name: thcD [H]

Synonym: Smed_0407

Alternate gene names: 150395632

Gene position: 436959-438239 (Clockwise)

Preceding gene: 150395631

Following gene: 150395635

Centisome position: 11.55

GC content: 63.62

Gene sequence:

>1281_bases
ATGGACCGGCCACGGGCCGGGAGGATAGCGATCAACACGAACCTTTTGAGACGGATGGAGCAGGTGTCGGGAAGACTTGT
TGTTGTCGGTGGCGGTCAAGCCGCATTCGCTTTGGTCGCCAAGCTTCGCGCCCTCAAGGACATGCGTCCGATCACCGTCG
TCGCGGCCGAAGCCAGCCTGCCTTACCAGCGGCCACCGCTTTCCAAGAAATATCTGTTGCGCGAAATGACGCTCGATCGC
CTGCTTTACCGGCCGGAGACCTGGTATGCGGAGCACGAGATCGATATCCGTCTTTCGACGACGGTCACGCGGGTCGATCG
TGCCACACGGCAGGTGGTGCTCAGCGATGGTTCGACGCTCGGCTATGAGACGCTTGCCTTCGCAACCGGCGCAACGCCAC
GACGGCTGCCGGCTGCGGTGGGTGGCGATCTTGCCGGCGTGTATGTGGTGCGCGACTTCAGGGATGCCGACCTGCTGGCG
GAGGAAATGCAGCCCGGCCGGCGAGTGCTGGTCGTCGGCGGCGGCTATATCGGGCTCGAGGCTGCAGCGGTTGCCCGCAT
CTCAGGGCTCGAGGTGACCGTCATCGAAATGGCCGACCGCATCCTTCAGCGCGTCGCCTCGGCCGCCACCTCGGCGATCG
TGCGCGAAATTCACAGCGCGCACGGCGTGCATATACGGGAAGGCACGGGGCTCCACCGTCTGATCGGCCATAACGGACGC
GTCACGGCGGCGGAGCTCTCCGACGGTTCCGTCATTCCCGTCGACATCGTGATCGTCGGAATTGGCGTGACGGCCAATGA
TGACCTGGCGCACGACGCCGGTATCGAAACGGCCAACGGGATCGTCGTCGACAGCCATGGGCGAACATCCGATCCCGCTG
TCTTCGCCATGGGAGACTGCGCTGTACTGCCATGGGACGGCATGCGTATCCGCCTCGAATCCGTGCAGAATGCAGTCGAC
CAGGCAGAGGCGGTTGCCGCGGTCCTCGCCGGCAGCAGCGTTCCCTATGCACCGAAACCCTGGTTCTGGTCCGACCAGTA
CGATGTCAAACTGCAGATCGCCGGTTTCGGTCTCGGCCATGACGAAACGCTGGTTCGGCCGGGCCAGCGCGAAGGCAGCG
TATCGGTCTGGTATTTCCGCCAGGGCAAGCTGATCGCCGTCGACGCGATAAACGACGCCAAGGCCTACGTCACCGGCAAG
AAGCTGCTCGAGCAGGGAGTGACGCCCAATCGAGCGCTGTTGGCGGATCCCCAGGTCGATCTCAAGACATTGCTGGCATA
G

Upstream 100 bases:

>100_bases
CATGGCATGGGTGGATCGCGGCCTGGCGCGCCCGGCCACGCAGAAGGGCATGGAGATCCCGCGCAAGGAGTGATTTGCAG
CGGGAACCTTCCGGCCTGAG

Downstream 100 bases:

>100_bases
CACCCGGGGTATGCTGCGGCCGCTACGGCCAGCACCATAAATCAAAAAGGGGCGGCTCGAGAGCCGCCCCTTTGATCCGG
ATGGATCTAATCGTGCTAAG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MDRPRAGRIAINTNLLRRMEQVSGRLVVVGGGQAAFALVAKLRALKDMRPITVVAAEASLPYQRPPLSKKYLLREMTLDR
LLYRPETWYAEHEIDIRLSTTVTRVDRATRQVVLSDGSTLGYETLAFATGATPRRLPAAVGGDLAGVYVVRDFRDADLLA
EEMQPGRRVLVVGGGYIGLEAAAVARISGLEVTVIEMADRILQRVASAATSAIVREIHSAHGVHIREGTGLHRLIGHNGR
VTAAELSDGSVIPVDIVIVGIGVTANDDLAHDAGIETANGIVVDSHGRTSDPAVFAMGDCAVLPWDGMRIRLESVQNAVD
QAEAVAAVLAGSSVPYAPKPWFWSDQYDVKLQIAGFGLGHDETLVRPGQREGSVSVWYFRQGKLIAVDAINDAKAYVTGK
KLLEQGVTPNRALLADPQVDLKTLLA

Sequences:

>Translated_426_residues
MDRPRAGRIAINTNLLRRMEQVSGRLVVVGGGQAAFALVAKLRALKDMRPITVVAAEASLPYQRPPLSKKYLLREMTLDR
LLYRPETWYAEHEIDIRLSTTVTRVDRATRQVVLSDGSTLGYETLAFATGATPRRLPAAVGGDLAGVYVVRDFRDADLLA
EEMQPGRRVLVVGGGYIGLEAAAVARISGLEVTVIEMADRILQRVASAATSAIVREIHSAHGVHIREGTGLHRLIGHNGR
VTAAELSDGSVIPVDIVIVGIGVTANDDLAHDAGIETANGIVVDSHGRTSDPAVFAMGDCAVLPWDGMRIRLESVQNAVD
QAEAVAAVLAGSSVPYAPKPWFWSDQYDVKLQIAGFGLGHDETLVRPGQREGSVSVWYFRQGKLIAVDAINDAKAYVTGK
KLLEQGVTPNRALLADPQVDLKTLLA
>Mature_426_residues
MDRPRAGRIAINTNLLRRMEQVSGRLVVVGGGQAAFALVAKLRALKDMRPITVVAAEASLPYQRPPLSKKYLLREMTLDR
LLYRPETWYAEHEIDIRLSTTVTRVDRATRQVVLSDGSTLGYETLAFATGATPRRLPAAVGGDLAGVYVVRDFRDADLLA
EEMQPGRRVLVVGGGYIGLEAAAVARISGLEVTVIEMADRILQRVASAATSAIVREIHSAHGVHIREGTGLHRLIGHNGR
VTAAELSDGSVIPVDIVIVGIGVTANDDLAHDAGIETANGIVVDSHGRTSDPAVFAMGDCAVLPWDGMRIRLESVQNAVD
QAEAVAAVLAGSSVPYAPKPWFWSDQYDVKLQIAGFGLGHDETLVRPGQREGSVSVWYFRQGKLIAVDAINDAKAYVTGK
KLLEQGVTPNRALLADPQVDLKTLLA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=374, Percent_Identity=26.2032085561497, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI226437568, Length=374, Percent_Identity=26.2032085561497, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI21389617, Length=374, Percent_Identity=26.2032085561497, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4757732, Length=336, Percent_Identity=25.297619047619, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI22202629, Length=336, Percent_Identity=25.297619047619, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788892, Length=404, Percent_Identity=37.1287128712871, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1789065, Length=320, Percent_Identity=30, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=29.2253521126761, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=382, Percent_Identity=29.0575916230367, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24639257, Length=414, Percent_Identity=25.6038647342995, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281359715, Length=414, Percent_Identity=25.8454106280193, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281359713, Length=414, Percent_Identity=25.8454106280193, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24639250, Length=414, Percent_Identity=25.8454106280193, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18543267, Length=414, Percent_Identity=25.8454106280193, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24639252, Length=414, Percent_Identity=25.8454106280193, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585130, Length=401, Percent_Identity=25.6857855361596, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=26.0504201680672, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=26.0504201680672, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 45841; Mature: 45841

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPRAGRIAINTNLLRRMEQVSGRLVVVGGGQAAFALVAKLRALKDMRPITVVAAEASL
CCCCCCCEEEECHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCC
PYQRPPLSKKYLLREMTLDRLLYRPETWYAEHEIDIRLSTTVTRVDRATRQVVLSDGSTL
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEECEEEEEEEEHHHHHHHHHHHEEEEECCCCC
GYETLAFATGATPRRLPAAVGGDLAGVYVVRDFRDADLLAEEMQPGRRVLVVGGGYIGLE
CCCCEEEECCCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHCCCCCEEEEECCCEECHH
AAAVARISGLEVTVIEMADRILQRVASAATSAIVREIHSAHGVHIREGTGLHRLIGHNGR
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCC
VTAAELSDGSVIPVDIVIVGIGVTANDDLAHDAGIETANGIVVDSHGRTSDPAVFAMGDC
EEEEECCCCCEEEEEEEEEEECCCCCCCCCHHCCCCCCCCEEEECCCCCCCCEEEEECCE
AVLPWDGMRIRLESVQNAVDQAEAVAAVLAGSSVPYAPKPWFWSDQYDVKLQIAGFGLGH
EEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
DETLVRPGQREGSVSVWYFRQGKLIAVDAINDAKAYVTGKKLLEQGVTPNRALLADPQVD
CCCEECCCCCCCCEEEEEEECCCEEEEEECCCCHHEEEHHHHHHCCCCCCCEEEECCCCC
LKTLLA
HHHHCC
>Mature Secondary Structure
MDRPRAGRIAINTNLLRRMEQVSGRLVVVGGGQAAFALVAKLRALKDMRPITVVAAEASL
CCCCCCCEEEECHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCC
PYQRPPLSKKYLLREMTLDRLLYRPETWYAEHEIDIRLSTTVTRVDRATRQVVLSDGSTL
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEECEEEEEEEEHHHHHHHHHHHEEEEECCCCC
GYETLAFATGATPRRLPAAVGGDLAGVYVVRDFRDADLLAEEMQPGRRVLVVGGGYIGLE
CCCCEEEECCCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHCCCCCEEEEECCCEECHH
AAAVARISGLEVTVIEMADRILQRVASAATSAIVREIHSAHGVHIREGTGLHRLIGHNGR
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCC
VTAAELSDGSVIPVDIVIVGIGVTANDDLAHDAGIETANGIVVDSHGRTSDPAVFAMGDC
EEEEECCCCCEEEEEEEEEEECCCCCCCCCHHCCCCCCCCEEEECCCCCCCCEEEEECCE
AVLPWDGMRIRLESVQNAVDQAEAVAAVLAGSSVPYAPKPWFWSDQYDVKLQIAGFGLGH
EEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
DETLVRPGQREGSVSVWYFRQGKLIAVDAINDAKAYVTGKKLLEQGVTPNRALLADPQVD
CCCEECCCCCCCCEEEEEEECCCEEEEEECCCCHHEEEHHHHHHCCCCCCCEEEECCCCC
LKTLLA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]