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The map label for this gene is hisS [H]

Identifier: 150395628

GI number: 150395628

Start: 432749

End: 434272

Strand: Direct

Name: hisS [H]

Synonym: Smed_0403

Alternate gene names: 150395628

Gene position: 432749-434272 (Clockwise)

Preceding gene: 150395624

Following gene: 150395629

Centisome position: 11.44

GC content: 62.34

Gene sequence:

>1524_bases
ATGCTTTCCATGAATGAAAAACAGAAGAAAACGCAGAAGCTCAGGGCGCGCTTGCCGCGTGGTTTCGTAGATCGTCCGGC
CGCGGACATCCGCGCCGTGGACGAGATGGTTTCGAAGATCCGCGAGGTCTATGAACGCTACGGCTTCGATCCGGTGGAAA
CCCCGCTTTTTGAATATACTGACGCGCTCGGAAAATTCCTGCCGGACAGCGACCGGCCGAATGAAGGCGTCTTCTCGCTC
ACCGACGACGACGACCAGTGGCTTAGTCTTCGCTACGACCTGACGGCGCCGCTGGCGCGCCACGTGGCCGAGAATTTCAA
CGAGATCCAGCTTCCCTACCGGACCTATCGCGCCGGCTACGTCTTCCGCAACGAGAAGCCGGGGCCGGGCCGTTTCCGCC
AGTTCATGCAGTTCGATGCCGATACGGTGGGTGCCGCCGGCGTGCAGGCCGATGCCGAAATGTGCATGATGATGGCCGAC
ACGATGGAGGCGCTCGGCATTGCCCGCGGCGACTACGTGATCCGCGTCAACAACCGCAAGGTTCTCGACGGCGTTCTGGA
GGCGATAGGTCTCGGCGGCGGCGAGCAGATGAATACGCGCCTGACGGTGCTGCGTGCCATCGACAAGCTCGACAAATTCG
GCCCGGAAGGCGTGCGGCTGTTGCTGGGCGAAGGACGCAAGGACGAGAGCGGCGACTTCACCAAGGGCGCCGGGCTCGAC
GATGAGCAGATCGGCAAGATCCTGTTCTTTGTCGGCATCACCGACTATGCCGAGAGCGCGGACGCGTTGGCGGCACTCGT
CGCCGGGACGGCCCGGGGCGCCGAGGGCGTCAATGAGTTGAACACGATCCGCAGCCTTGTGCTCAGCGCCGGCTACGAGG
CGGACCGCATCAAGATCGACCCCTCGGTCGTGCGTGGTCTGGAATATTATACGGGTCCCGTTTTCGAGGCGGAGTTGCAG
TTCGCCGTCACCAACGAAAAGGGGGAGAAGGTCGTCTTCGGCTCCGTCGGCGGAGGCGGCCGCTATGACGGTCTCGTGTC
GCGCTTCATGGGACAGCCGGTGCCGGCGACCGGCTTTTCGATCGGCGTGTCTCGGCTGATGACGGCGCTCAAGAATCTGG
GCAAACTGGGCGTAGAACAGGTCACCGCGCCGGTCGTCGTTTGCGTCATGGACCGCGACATCGAAAGCATGGGGCGCTAC
CAGCGCTTTGTGCAGGATCTGCGTCATGCCGGCATCCGCGCCGAAATGTACCAGGGCAACAAGAAGAACTTCGGCGACCA
GCTCAAATATGCCGATCGTCGCGGTTCGCCGATCGCTGTCATTCAGGGCGGCGACGAGCGCGCTTCCGGCGTCGTACAGA
TCAAGGACCTGATCGAAGGCAAGCGGCTCTCTGGCGAAATCGAAGATAATGTCGCTTGGCGCGAGGCGCGTGCCGCTCAG
ATTTCCGTCCCGGAAGGCGAACTCGTCGTTAAGATTCGCGAAATTCTCGCGGACCAGGCCGAGGACCGGAAAAGGGCGGG
TTGA

Upstream 100 bases:

>100_bases
GTTCCGAAAGGGAGCCTATGGGCGAGGCCTGAGGGCGGTCGCCCTTGCCGCTTCTGCCATGATCCGGTAGGGAAGGCACC
GACCAAGCACGAAGTCCGGA

Downstream 100 bases:

>100_bases
CCGCGATGCCTCTCATTGATCTTCCCGGTTTCGCCGGCGACCTCCTTGCGGATTTCGAACGCCGGAACACGCTGCGCGTC
GACACGCCGGTCATCCAGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLSMNEKQKKTQKLRARLPRGFVDRPAADIRAVDEMVSKIREVYERYGFDPVETPLFEYTDALGKFLPDSDRPNEGVFSL
TDDDDQWLSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFRNEKPGPGRFRQFMQFDADTVGAAGVQADAEMCMMMAD
TMEALGIARGDYVIRVNNRKVLDGVLEAIGLGGGEQMNTRLTVLRAIDKLDKFGPEGVRLLLGEGRKDESGDFTKGAGLD
DEQIGKILFFVGITDYAESADALAALVAGTARGAEGVNELNTIRSLVLSAGYEADRIKIDPSVVRGLEYYTGPVFEAELQ
FAVTNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGVSRLMTALKNLGKLGVEQVTAPVVVCVMDRDIESMGRY
QRFVQDLRHAGIRAEMYQGNKKNFGDQLKYADRRGSPIAVIQGGDERASGVVQIKDLIEGKRLSGEIEDNVAWREARAAQ
ISVPEGELVVKIREILADQAEDRKRAG

Sequences:

>Translated_507_residues
MLSMNEKQKKTQKLRARLPRGFVDRPAADIRAVDEMVSKIREVYERYGFDPVETPLFEYTDALGKFLPDSDRPNEGVFSL
TDDDDQWLSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFRNEKPGPGRFRQFMQFDADTVGAAGVQADAEMCMMMAD
TMEALGIARGDYVIRVNNRKVLDGVLEAIGLGGGEQMNTRLTVLRAIDKLDKFGPEGVRLLLGEGRKDESGDFTKGAGLD
DEQIGKILFFVGITDYAESADALAALVAGTARGAEGVNELNTIRSLVLSAGYEADRIKIDPSVVRGLEYYTGPVFEAELQ
FAVTNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGVSRLMTALKNLGKLGVEQVTAPVVVCVMDRDIESMGRY
QRFVQDLRHAGIRAEMYQGNKKNFGDQLKYADRRGSPIAVIQGGDERASGVVQIKDLIEGKRLSGEIEDNVAWREARAAQ
ISVPEGELVVKIREILADQAEDRKRAG
>Mature_507_residues
MLSMNEKQKKTQKLRARLPRGFVDRPAADIRAVDEMVSKIREVYERYGFDPVETPLFEYTDALGKFLPDSDRPNEGVFSL
TDDDDQWLSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFRNEKPGPGRFRQFMQFDADTVGAAGVQADAEMCMMMAD
TMEALGIARGDYVIRVNNRKVLDGVLEAIGLGGGEQMNTRLTVLRAIDKLDKFGPEGVRLLLGEGRKDESGDFTKGAGLD
DEQIGKILFFVGITDYAESADALAALVAGTARGAEGVNELNTIRSLVLSAGYEADRIKIDPSVVRGLEYYTGPVFEAELQ
FAVTNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGVSRLMTALKNLGKLGVEQVTAPVVVCVMDRDIESMGRY
QRFVQDLRHAGIRAEMYQGNKKNFGDQLKYADRRGSPIAVIQGGDERASGVVQIKDLIEGKRLSGEIEDNVAWREARAAQ
ISVPEGELVVKIREILADQAEDRKRAG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=481, Percent_Identity=29.5218295218295, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI6996014, Length=483, Percent_Identity=30.0207039337474, Blast_Score=176, Evalue=5e-44,
Organism=Escherichia coli, GI1788861, Length=471, Percent_Identity=27.8131634819533, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993693, Length=445, Percent_Identity=28.7640449438202, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71993686, Length=445, Percent_Identity=28.7640449438202, Blast_Score=162, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6325290, Length=487, Percent_Identity=28.3367556468172, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643061, Length=488, Percent_Identity=30.7377049180328, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24643059, Length=507, Percent_Identity=29.3885601577909, Blast_Score=168, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 55892; Mature: 55892

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSMNEKQKKTQKLRARLPRGFVDRPAADIRAVDEMVSKIREVYERYGFDPVETPLFEYT
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DALGKFLPDSDRPNEGVFSLTDDDDQWLSLRYDLTAPLARHVAENFNEIQLPYRTYRAGY
HHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHCCCCEEECCHHHHHCCE
VFRNEKPGPGRFRQFMQFDADTVGAAGVQADAEMCMMMADTMEALGIARGDYVIRVNNRK
EEECCCCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
VLDGVLEAIGLGGGEQMNTRLTVLRAIDKLDKFGPEGVRLLLGEGRKDESGDFTKGAGLD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
DEQIGKILFFVGITDYAESADALAALVAGTARGAEGVNELNTIRSLVLSAGYEADRIKID
HHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEC
PSVVRGLEYYTGPVFEAELQFAVTNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFS
HHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHH
IGVSRLMTALKNLGKLGVEQVTAPVVVCVMDRDIESMGRYQRFVQDLRHAGIRAEMYQGN
HHHHHHHHHHHHHHHHCHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEHHCCC
KKNFGDQLKYADRRGSPIAVIQGGDERASGVVQIKDLIEGKRLSGEIEDNVAWREARAAQ
CCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHCCCCCCCCHHCCHHHHHHCCEE
ISVPEGELVVKIREILADQAEDRKRAG
EECCCCHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MLSMNEKQKKTQKLRARLPRGFVDRPAADIRAVDEMVSKIREVYERYGFDPVETPLFEYT
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DALGKFLPDSDRPNEGVFSLTDDDDQWLSLRYDLTAPLARHVAENFNEIQLPYRTYRAGY
HHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHCCCCEEECCHHHHHCCE
VFRNEKPGPGRFRQFMQFDADTVGAAGVQADAEMCMMMADTMEALGIARGDYVIRVNNRK
EEECCCCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
VLDGVLEAIGLGGGEQMNTRLTVLRAIDKLDKFGPEGVRLLLGEGRKDESGDFTKGAGLD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
DEQIGKILFFVGITDYAESADALAALVAGTARGAEGVNELNTIRSLVLSAGYEADRIKID
HHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEC
PSVVRGLEYYTGPVFEAELQFAVTNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFS
HHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHH
IGVSRLMTALKNLGKLGVEQVTAPVVVCVMDRDIESMGRYQRFVQDLRHAGIRAEMYQGN
HHHHHHHHHHHHHHHHCHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEHHCCC
KKNFGDQLKYADRRGSPIAVIQGGDERASGVVQIKDLIEGKRLSGEIEDNVAWREARAAQ
CCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHCCCCCCCCHHCCHHHHHHCCEE
ISVPEGELVVKIREILADQAEDRKRAG
EECCCCHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]