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The map label for this gene is tag [H]

Identifier: 150395624

GI number: 150395624

Start: 428776

End: 429411

Strand: Direct

Name: tag [H]

Synonym: Smed_0399

Alternate gene names: 150395624

Gene position: 428776-429411 (Clockwise)

Preceding gene: 150395621

Following gene: 150395628

Centisome position: 11.34

GC content: 60.85

Gene sequence:

>636_bases
ATGGCAGCAAAGGGTTTGATTACGGGGGATGACGGGCTCGATCGTTGCGCCTGGCATGGCAATCTGGAGGATTACCGCCG
CTATCACGACGAGGAATGGGGCCGGCCTGTCGCCGATGATCATAGGTTGTTCGAAAAGATCTGCCTCGAAGGCTTCCAGT
CGGGCCTCTCATGGCTGACGATCCTGAGAAAGCGCGAGGCGTTTCGCGCCGCATTCGCGGGCTTCGCCTTCGACAGGGTT
GCCGAGTTCGGAGAGGAGGACGTCGAGCGATGCCTTGCCGATGCCGGCATCGTCCGCCACCGCGGCAAGATCGTCTCGAC
CATCAATAATGCTCGCCGTGCAAAGGAGCTGCGTTCGGAATTCGGATCGCTCGCGGCCTATTTCTGGTCGCATGAACCCG
AAAGCAGCGAGCGTCCGGCAGTCGTCGATCAGGCCGCGCTCATCGCCAATCCGACGACGCCTGCATCGATCAGGATTTCG
AAGGATCTGAAGAAGCGCGGCTGGACTTTCGTCGGTCCGACGACCGTCTATGCTTTCATGCAGGCGATGGGCCTCGTCAA
CGACCACATAGAGGGCTGTTTCTGCCGTGCTCCGATAGAGGAGCTTCGCCGCCGCTTCACACGCCCGGCGGCATGA

Upstream 100 bases:

>100_bases
CCGGCGCAATAAAGCTCTGGGTAGGGTTATTCGCCCCTTTATCGATGTGGTTTGCAGATGGTAAACAGAACAAAATGGAA
ACAACGGAGAGAGGCGGAGG

Downstream 100 bases:

>100_bases
TTGGCGCGTCGGAGACGAGCGGCGCCCTCCCCAACGGGAGGAGGGCGCTTTGGTGGGAAAGGCACCAGGTCAGGCAGGGT
AGGGCCTGGCTGATCTGAAG

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAAKGLITGDDGLDRCAWHGNLEDYRRYHDEEWGRPVADDHRLFEKICLEGFQSGLSWLTILRKREAFRAAFAGFAFDRV
AEFGEEDVERCLADAGIVRHRGKIVSTINNARRAKELRSEFGSLAAYFWSHEPESSERPAVVDQAALIANPTTPASIRIS
KDLKKRGWTFVGPTTVYAFMQAMGLVNDHIEGCFCRAPIEELRRRFTRPAA

Sequences:

>Translated_211_residues
MAAKGLITGDDGLDRCAWHGNLEDYRRYHDEEWGRPVADDHRLFEKICLEGFQSGLSWLTILRKREAFRAAFAGFAFDRV
AEFGEEDVERCLADAGIVRHRGKIVSTINNARRAKELRSEFGSLAAYFWSHEPESSERPAVVDQAALIANPTTPASIRIS
KDLKKRGWTFVGPTTVYAFMQAMGLVNDHIEGCFCRAPIEELRRRFTRPAA
>Mature_210_residues
AAKGLITGDDGLDRCAWHGNLEDYRRYHDEEWGRPVADDHRLFEKICLEGFQSGLSWLTILRKREAFRAAFAGFAFDRVA
EFGEEDVERCLADAGIVRHRGKIVSTINNARRAKELRSEFGSLAAYFWSHEPESSERPAVVDQAALIANPTTPASIRISK
DLKKRGWTFVGPTTVYAFMQAMGLVNDHIEGCFCRAPIEELRRRFTRPAA

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=184, Percent_Identity=49.4565217391304, Blast_Score=176, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 23892; Mature: 23761

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKGLITGDDGLDRCAWHGNLEDYRRYHDEEWGRPVADDHRLFEKICLEGFQSGLSWLT
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILRKREAFRAAFAGFAFDRVAEFGEEDVERCLADAGIVRHRGKIVSTINNARRAKELRSE
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSLAAYFWSHEPESSERPAVVDQAALIANPTTPASIRISKDLKKRGWTFVGPTTVYAFM
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHCCCEEECHHHHHHHH
QAMGLVNDHIEGCFCRAPIEELRRRFTRPAA
HHHHHHHHHHCCCHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AAKGLITGDDGLDRCAWHGNLEDYRRYHDEEWGRPVADDHRLFEKICLEGFQSGLSWLT
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILRKREAFRAAFAGFAFDRVAEFGEEDVERCLADAGIVRHRGKIVSTINNARRAKELRSE
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSLAAYFWSHEPESSERPAVVDQAALIANPTTPASIRISKDLKKRGWTFVGPTTVYAFM
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHCCCEEECHHHHHHHH
QAMGLVNDHIEGCFCRAPIEELRRRFTRPAA
HHHHHHHHHHCCCHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]