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The map label for this gene is potI [H]

Identifier: 150395540

GI number: 150395540

Start: 337314

End: 338135

Strand: Direct

Name: potI [H]

Synonym: Smed_0313

Alternate gene names: 150395540

Gene position: 337314-338135 (Clockwise)

Preceding gene: 150395539

Following gene: 150395542

Centisome position: 8.92

GC content: 62.29

Gene sequence:

>822_bases
ATGGAAAAGTGGTCACGATTCAATATCGCCTCCGTCGTTCTCGGCTTCGGCTTTCTCTATCTGCCGATCGTGCTCCTGGT
GATCTTCTCCTTCAACGAGTCGAAGCTGGTAACGGTCTGGGCGGGGTTTTCCACCAAGTGGTACGGCCAGCTCTGGCAGA
ATCAAAGCCTTCTGGACGCGGCATGGGTCACGATCCGGGTCGCACTCCTTTCGGCAACCTGCGCGACCGTGCTGGGAACG
ATGGCCGCGCTCGCGCTGGTGCGCTACACCCGCTTCCGCGGGCGCGTTCTCTTTTCCGGCATGGTCTATGCGCCCCTGGT
GATGCCGGAAGTGATAACCGGACTCTCGCTGCTTCTTCTCTTCGTGGCGATCGGGTTTGACCGTGGCTTCTGGACGATCA
CGCTCGCCCACATCACCTTCACCATGTGTTTCGTCGCGGTCGTCGTGCAGTCGCGTCTTCTGAGCTTCGATCAGTCGATC
GAGGAGGCCGCGCTCGATCTCGGCGCGACGCCGGTCAGGACCTTCTTCGCGATCACCCTGCCGGTGATCGCCCCGGCGGT
CTTTTCCGGCTGGATACTCGCTTTCACGCTGTCGCTGGACGACCTGGTGATCGCAAGCTTCACGACCGGGCCCGGCGCGA
CGACGCTGCCGATGAAGATCTACAGCCAGGTCCGCCTCGGCGTGACGCCGGAAATTAACGCAATCTGCACGATCCTGATC
GGGGTCGTTGCCCTGGGCGTGATCGTCGCATCCATCGTTACGAGGCGTCGCGAAGCCCAGCGGGAGAAGGATGAGCGGGC
CGCCTTCGCGGCCATCGGGTGA

Upstream 100 bases:

>100_bases
ATCGTCGGCAGTGGCGATCATCCTGCTGATGATCCTGGTGATACCGATCGTATATTTCCAGAACATCCAGGCCAAAGCCG
ACGGCGAGGGAAGGTGAGAC

Downstream 100 bases:

>100_bases
GTTGTCAGTTCGGGCCAAGGAGGACTGAAAGCGGCGAGATCACCGCATTCGGCCAGGAGCCGCCGATGAAGAACTGCAGT
GGTTCGTCGTCGTCTGCCGG

Product: ornithine carbamoyltransferase

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEKWSRFNIASVVLGFGFLYLPIVLLVIFSFNESKLVTVWAGFSTKWYGQLWQNQSLLDAAWVTIRVALLSATCATVLGT
MAALALVRYTRFRGRVLFSGMVYAPLVMPEVITGLSLLLLFVAIGFDRGFWTITLAHITFTMCFVAVVVQSRLLSFDQSI
EEAALDLGATPVRTFFAITLPVIAPAVFSGWILAFTLSLDDLVIASFTTGPGATTLPMKIYSQVRLGVTPEINAICTILI
GVVALGVIVASIVTRRREAQREKDERAAFAAIG

Sequences:

>Translated_273_residues
MEKWSRFNIASVVLGFGFLYLPIVLLVIFSFNESKLVTVWAGFSTKWYGQLWQNQSLLDAAWVTIRVALLSATCATVLGT
MAALALVRYTRFRGRVLFSGMVYAPLVMPEVITGLSLLLLFVAIGFDRGFWTITLAHITFTMCFVAVVVQSRLLSFDQSI
EEAALDLGATPVRTFFAITLPVIAPAVFSGWILAFTLSLDDLVIASFTTGPGATTLPMKIYSQVRLGVTPEINAICTILI
GVVALGVIVASIVTRRREAQREKDERAAFAAIG
>Mature_273_residues
MEKWSRFNIASVVLGFGFLYLPIVLLVIFSFNESKLVTVWAGFSTKWYGQLWQNQSLLDAAWVTIRVALLSATCATVLGT
MAALALVRYTRFRGRVLFSGMVYAPLVMPEVITGLSLLLLFVAIGFDRGFWTITLAHITFTMCFVAVVVQSRLLSFDQSI
EEAALDLGATPVRTFFAITLPVIAPAVFSGWILAFTLSLDDLVIASFTTGPGATTLPMKIYSQVRLGVTPEINAICTILI
GVVALGVIVASIVTRRREAQREKDERAAFAAIG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=270, Percent_Identity=58.1481481481482, Blast_Score=273, Evalue=9e-75,
Organism=Escherichia coli, GI1787368, Length=237, Percent_Identity=45.5696202531646, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1787714, Length=247, Percent_Identity=33.1983805668016, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788764, Length=179, Percent_Identity=32.4022346368715, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29841; Mature: 29841

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKWSRFNIASVVLGFGFLYLPIVLLVIFSFNESKLVTVWAGFSTKWYGQLWQNQSLLDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCHHHHH
AWVTIRVALLSATCATVLGTMAALALVRYTRFRGRVLFSGMVYAPLVMPEVITGLSLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHH
FVAIGFDRGFWTITLAHITFTMCFVAVVVQSRLLSFDQSIEEAALDLGATPVRTFFAITL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
PVIAPAVFSGWILAFTLSLDDLVIASFTTGPGATTLPMKIYSQVRLGVTPEINAICTILI
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
GVVALGVIVASIVTRRREAQREKDERAAFAAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKWSRFNIASVVLGFGFLYLPIVLLVIFSFNESKLVTVWAGFSTKWYGQLWQNQSLLDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCHHHHH
AWVTIRVALLSATCATVLGTMAALALVRYTRFRGRVLFSGMVYAPLVMPEVITGLSLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHH
FVAIGFDRGFWTITLAHITFTMCFVAVVVQSRLLSFDQSIEEAALDLGATPVRTFFAITL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
PVIAPAVFSGWILAFTLSLDDLVIASFTTGPGATTLPMKIYSQVRLGVTPEINAICTILI
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
GVVALGVIVASIVTRRREAQREKDERAAFAAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]