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The map label for this gene is acsA1 [H]

Identifier: 150395542

GI number: 150395542

Start: 340138

End: 342090

Strand: Direct

Name: acsA1 [H]

Synonym: Smed_0315

Alternate gene names: 150395542

Gene position: 340138-342090 (Clockwise)

Preceding gene: 150395540

Following gene: 150395545

Centisome position: 8.99

GC content: 62.52

Gene sequence:

>1953_bases
ATGCAAGCAGATCTGCCTTTGTGGGTGCCGGACAGGGAGATTCTCGAACATAGCCCGATGGCTGAGTTCATCGCCTGGTG
CGGGACGCGCTTCAAAGCGAGCTTCGCCGATTATGACGCCTTTCATGACTGGTCGGTGAGTGAGCGCGGACACTTCTGGA
CCGCCGTATGGGAGCATTGCAAGGTCATAGGTGAGCCCGGGGAGAGGGCACTCGTCGATGGCGAGCGAATGCTCGACGCC
CGTTTCTTCCCGGATGCGAGGCTCAACTTTGCCGAAAATCTGCTGCGCAAGACGGGCAGCGGTGCCGCCCTGATCTTCCG
CGGCGAGGACAAGGTGAGCTATCAGTTGAGTTGGGCCGAATTGCGCGCCCTGGTATCGCGCCTGCAACAGGCGCTGAAGG
CGCAAGGGATCGGCGTCGGGGATCGCGTTGCCGCGATGATGCCGAATATGCCGGAAACAATTGCCCTCATGCTGGCGACC
GCTTCCGTAGGCGCCATATGGTCGTCCTGCTCGCCCGATTTCGGCGAGCAGGGCGTTCTCGATCGCTTCGGCCAGATAGC
GCCCAAGCTCTTCATCGCATGCGACGGTTATTGGTATAACGGCAAGCGGCAGGATGTGGACGCGAAGGTGCGCGCCGTGG
CCAAGGCGCTCGGCGTCCCGACCGTCATCGTTCCCTATGCCGGCGACAGTGCCGCACTCGCGCCTACCGTGGAGCGCGGG
TTGACGCTTGCCGATTTCATCGCCGATTTCGCCGCAGAACCGCTCGAATTCGAGCGACTGCCATTCAATCAGCCGCTCTA
TATTCTGTTCTCCTCCGGCACCACCGGCGTGCCGAAATGCATCGTCCATTCGGCCGGCGGAACGCTGCTGCAGCACCTTA
AGGAGCACCGTTTCCACTGTGGCCTGAGGGAAGGGGAGCGGCTGTTCTACTTCACCACCTGCGGCTGGATGATGTGGAAC
TGGCTGGCTTCGGGTCTGGCTGTGGGCGCAACGCTCTGCCTCTATGACGGCTCGCCTTTCTACCCGGACGGCAACGTGCT
CTTCGATTTTGCCGCCGCTGAGCGCTTCGCCGTGTTCGGAACGTCGGCGAAATATATCGATGCGGTGCGAAAGGGCGGCC
TCACCCCGTCGAAGACGCACGATTTGTCGCCCCTGCGGCTCATGACCTCCACCGGCTCGCCGCTCTCGCCCGAAGGCTTT
TCCTTCGTCTACGAGGGCATCAAGTCCGATGTCCAGCTCGCGTCGATTTCCGGCGGGACCGACATCGTCTCCTGCTTCGT
GCTCGGCAATCCCATGAAGCCCGTATGGCGCGGAGAGATCCAGGGCTCCGGCCTTGGCCTCGCCGTCGACGTCTGGAACG
ACGAAGGCAGACCCGTGCGCGGCGAAAAGGGCGAACTCGTCTGCACCAGGGCATTTCCGTCCATGCCCGTCATGTTCTGG
AACGATCCCGACGGAGCGAAATATCGAGCCGCCTATTTCGACCGCTTCGACAATATCTGGTGCCATGGCGATTTTGCCGA
ATGGACCCCGCATGGCGGCATCGTCATCCATGGCCGCTCCGACGCAACACTGAATCCCGGGGGCGTGCGCATCGGCACGG
CGGAGATCTACAATCAGGTCGAGCAGATGGAAGAAGTGGCAGAGGCGCTCTGCATCGGCCAGGACTGGGAGGATGACGTC
CGCGTCGTCCTTTTCGTGCGGCTGGCGCAAGGCGCCGAACTCACAGAGCAACTGTCCAGGGAAATCAAAAACCGGATTCG
ATCCGGCGCCTCGCCGCGGCACGTACCGGCGAAGATCATCGCTGTCGCCGACATCCCGCGCACCAAGTCCGGCAAGATCG
TCGAACTGGCGGTCCGCGACGTCGTGCACGGGCGCCCCGTGAAGAACAAGGAAGCGCTGGCGAACCCAGAGGCTCTCGAC
CTCTTCGCCGGGCTGGAGGCGCTGAAGAGCTGA

Upstream 100 bases:

>100_bases
CCGAGATGGAATGTCGCCGCAGCGACGATGCCGAGCCGCATCCTCGCTGTTTGTTTCGCGCTGCAGCATGATATAGAAAG
GCCAAAGAGTGGAGGAGAAC

Downstream 100 bases:

>100_bases
TCGACACCGTGCTGGGCGCCAATCCGCTCAGCAGGGTGTCGCTCCCGAAGGGGGAGCCGTCTTTTCTTCGCTGCTCGGGA
GCCATATTTATATATATATC

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MQADLPLWVPDREILEHSPMAEFIAWCGTRFKASFADYDAFHDWSVSERGHFWTAVWEHCKVIGEPGERALVDGERMLDA
RFFPDARLNFAENLLRKTGSGAALIFRGEDKVSYQLSWAELRALVSRLQQALKAQGIGVGDRVAAMMPNMPETIALMLAT
ASVGAIWSSCSPDFGEQGVLDRFGQIAPKLFIACDGYWYNGKRQDVDAKVRAVAKALGVPTVIVPYAGDSAALAPTVERG
LTLADFIADFAAEPLEFERLPFNQPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEHRFHCGLREGERLFYFTTCGWMMWN
WLASGLAVGATLCLYDGSPFYPDGNVLFDFAAAERFAVFGTSAKYIDAVRKGGLTPSKTHDLSPLRLMTSTGSPLSPEGF
SFVYEGIKSDVQLASISGGTDIVSCFVLGNPMKPVWRGEIQGSGLGLAVDVWNDEGRPVRGEKGELVCTRAFPSMPVMFW
NDPDGAKYRAAYFDRFDNIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQMEEVAEALCIGQDWEDDV
RVVLFVRLAQGAELTEQLSREIKNRIRSGASPRHVPAKIIAVADIPRTKSGKIVELAVRDVVHGRPVKNKEALANPEALD
LFAGLEALKS

Sequences:

>Translated_650_residues
MQADLPLWVPDREILEHSPMAEFIAWCGTRFKASFADYDAFHDWSVSERGHFWTAVWEHCKVIGEPGERALVDGERMLDA
RFFPDARLNFAENLLRKTGSGAALIFRGEDKVSYQLSWAELRALVSRLQQALKAQGIGVGDRVAAMMPNMPETIALMLAT
ASVGAIWSSCSPDFGEQGVLDRFGQIAPKLFIACDGYWYNGKRQDVDAKVRAVAKALGVPTVIVPYAGDSAALAPTVERG
LTLADFIADFAAEPLEFERLPFNQPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEHRFHCGLREGERLFYFTTCGWMMWN
WLASGLAVGATLCLYDGSPFYPDGNVLFDFAAAERFAVFGTSAKYIDAVRKGGLTPSKTHDLSPLRLMTSTGSPLSPEGF
SFVYEGIKSDVQLASISGGTDIVSCFVLGNPMKPVWRGEIQGSGLGLAVDVWNDEGRPVRGEKGELVCTRAFPSMPVMFW
NDPDGAKYRAAYFDRFDNIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQMEEVAEALCIGQDWEDDV
RVVLFVRLAQGAELTEQLSREIKNRIRSGASPRHVPAKIIAVADIPRTKSGKIVELAVRDVVHGRPVKNKEALANPEALD
LFAGLEALKS
>Mature_650_residues
MQADLPLWVPDREILEHSPMAEFIAWCGTRFKASFADYDAFHDWSVSERGHFWTAVWEHCKVIGEPGERALVDGERMLDA
RFFPDARLNFAENLLRKTGSGAALIFRGEDKVSYQLSWAELRALVSRLQQALKAQGIGVGDRVAAMMPNMPETIALMLAT
ASVGAIWSSCSPDFGEQGVLDRFGQIAPKLFIACDGYWYNGKRQDVDAKVRAVAKALGVPTVIVPYAGDSAALAPTVERG
LTLADFIADFAAEPLEFERLPFNQPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEHRFHCGLREGERLFYFTTCGWMMWN
WLASGLAVGATLCLYDGSPFYPDGNVLFDFAAAERFAVFGTSAKYIDAVRKGGLTPSKTHDLSPLRLMTSTGSPLSPEGF
SFVYEGIKSDVQLASISGGTDIVSCFVLGNPMKPVWRGEIQGSGLGLAVDVWNDEGRPVRGEKGELVCTRAFPSMPVMFW
NDPDGAKYRAAYFDRFDNIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQMEEVAEALCIGQDWEDDV
RVVLFVRLAQGAELTEQLSREIKNRIRSGASPRHVPAKIIAVADIPRTKSGKIVELAVRDVVHGRPVKNKEALANPEALD
LFAGLEALKS

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=657, Percent_Identity=43.9878234398782, Blast_Score=549, Evalue=1e-156,
Organism=Homo sapiens, GI8923896, Length=597, Percent_Identity=25.963149078727, Blast_Score=171, Evalue=3e-42,
Organism=Homo sapiens, GI253970489, Length=610, Percent_Identity=25.7377049180328, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI28416953, Length=567, Percent_Identity=25.3968253968254, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI13375727, Length=648, Percent_Identity=24.6913580246914, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI38505220, Length=511, Percent_Identity=22.7005870841487, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1790505, Length=580, Percent_Identity=26.8965517241379, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1786529, Length=647, Percent_Identity=24.2658423493045, Blast_Score=116, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17569507, Length=630, Percent_Identity=39.0476190476191, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71983001, Length=582, Percent_Identity=25.4295532646048, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71982997, Length=582, Percent_Identity=25.4295532646048, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=644, Percent_Identity=25.6211180124224, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323182, Length=660, Percent_Identity=24.5454545454545, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24667955, Length=651, Percent_Identity=25.3456221198157, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI62472339, Length=560, Percent_Identity=26.25, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24667959, Length=523, Percent_Identity=25.4302103250478, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI21355225, Length=633, Percent_Identity=24.0126382306477, Blast_Score=117, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 71373; Mature: 71373

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQADLPLWVPDREILEHSPMAEFIAWCGTRFKASFADYDAFHDWSVSERGHFWTAVWEHC
CCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KVIGEPGERALVDGERMLDARFFPDARLNFAENLLRKTGSGAALIFRGEDKVSYQLSWAE
HHCCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEECHHH
LRALVSRLQQALKAQGIGVGDRVAAMMPNMPETIALMLATASVGAIWSSCSPDFGEQGVL
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DRFGQIAPKLFIACDGYWYNGKRQDVDAKVRAVAKALGVPTVIVPYAGDSAALAPTVERG
HHHHHHCCEEEEEECCEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHC
LTLADFIADFAAEPLEFERLPFNQPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEHRFHC
CHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEECCCHHHHHHHHHHHHCC
GLREGERLFYFTTCGWMMWNWLASGLAVGATLCLYDGSPFYPDGNVLFDFAAAERFAVFG
CCCCCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEEEECCCCEEEEEC
TSAKYIDAVRKGGLTPSKTHDLSPLRLMTSTGSPLSPEGFSFVYEGIKSDVQLASISGGT
CCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCH
DIVSCFVLGNPMKPVWRGEIQGSGLGLAVDVWNDEGRPVRGEKGELVCTRAFPSMPVMFW
HHEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEE
NDPDGAKYRAAYFDRFDNIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQV
ECCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCEEEECCCCCEECCCCEEEEHHHHHHHH
EQMEEVAEALCIGQDWEDDVRVVLFVRLAQGAELTEQLSREIKNRIRSGASPRHVPAKII
HHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEE
AVADIPRTKSGKIVELAVRDVVHGRPVKNKEALANPEALDLFAGLEALKS
EEECCCCCCCCCEEEEEHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQADLPLWVPDREILEHSPMAEFIAWCGTRFKASFADYDAFHDWSVSERGHFWTAVWEHC
CCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KVIGEPGERALVDGERMLDARFFPDARLNFAENLLRKTGSGAALIFRGEDKVSYQLSWAE
HHCCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEECHHH
LRALVSRLQQALKAQGIGVGDRVAAMMPNMPETIALMLATASVGAIWSSCSPDFGEQGVL
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DRFGQIAPKLFIACDGYWYNGKRQDVDAKVRAVAKALGVPTVIVPYAGDSAALAPTVERG
HHHHHHCCEEEEEECCEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHC
LTLADFIADFAAEPLEFERLPFNQPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEHRFHC
CHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEECCCHHHHHHHHHHHHCC
GLREGERLFYFTTCGWMMWNWLASGLAVGATLCLYDGSPFYPDGNVLFDFAAAERFAVFG
CCCCCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEEEECCCCEEEEEC
TSAKYIDAVRKGGLTPSKTHDLSPLRLMTSTGSPLSPEGFSFVYEGIKSDVQLASISGGT
CCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCH
DIVSCFVLGNPMKPVWRGEIQGSGLGLAVDVWNDEGRPVRGEKGELVCTRAFPSMPVMFW
HHEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEE
NDPDGAKYRAAYFDRFDNIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQV
ECCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCEEEECCCCCEECCCCEEEEHHHHHHHH
EQMEEVAEALCIGQDWEDDVRVVLFVRLAQGAELTEQLSREIKNRIRSGASPRHVPAKII
HHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEE
AVADIPRTKSGKIVELAVRDVVHGRPVKNKEALANPEALDLFAGLEALKS
EEECCCCCCCCCEEEEEHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]