Click here to switch to the map view.

The map label for this gene is aglA [H]

Identifier: 150395522

GI number: 150395522

Start: 314094

End: 315749

Strand: Direct

Name: aglA [H]

Synonym: Smed_0295

Alternate gene names: 150395522

Gene position: 314094-315749 (Clockwise)

Preceding gene: 150395521

Following gene: 150395523

Centisome position: 8.31

GC content: 60.39

Gene sequence:

>1656_bases
ATGACACTGAGCGAAACGACGAGCTCCCTTCTCGAGCCGGACAGGGACTGGTGGCGCGGAGCGGTAATCTACCAGATCTA
TCCCCGTTCCTTCCAGGACACCAATGGCGACGGGATCGGGGATCTGCAGGGGATCACGGCTCGGTTGCCTCACATTGCCG
GGCTCGGCGCGGACGCGATCTGGATATCGCCCTTTTTCACGTCCCCGATGAAGGATTTCGGCTACGATGTCTCGAATTAT
GTCGATGTCGACCCGATCTTCGGCGCGCTCGACGACTTCGATGCGCTGATCGCCGAGGCGCATCGGCTCGGTCTTCGAGT
GATGATCGACCTCGTCCTGTCGCACAGCTCGGACCAGCATCCCTGGTTCGTCGAAAGCCGGTCGACCCGCAGCAATGCAA
AAGCGGATTGGTACGTCTGGGCGGATTCGAAGCCAGACGGAACACCTCCGAACAACTGGCTGTCGATTTTCGGCGGTTCC
GCCTGGCAATGGGATCCAACGCGGCTGCAATATTACCTGCACAATTTCCTGATCTCGCAGCCCGACCTCAATCTGCATAA
TTCCGAGGTTCAGGACGCACTGCTCTCTGTCGCACGCTTCTGGCTGGAGCGTGGCGTCGACGGTTTTCGGCTCGACACGA
TCAACTTCTATTTCCATGACCGTGAGCTGCGCGACAACCCGGCTCTGGCACCAGAACGCCGCAACGCCTCGACCGCGCCG
GCGGTTAATCCCTATAACTACCAGGAGCATATCTACGACAAGAACCGGCCGGAGAACCTGGAATTCCTGCAGCGCTTCCG
TGCGTTGATGGATGAGTTCCCGGCGATTGCCGCCGTCGGGGAAGTCGGCGACAGCCAGCGTGGTCTCGACATCGCCGCCG
AATACACCTCCGGTGGCGACAAGGTGCATATGTGCTACGCCTTCGAGTTCCTGGCACCGGATCAGCTGACGCCGCAAAGG
GTGGCCGAGGTCCTGCGCGACTTCCAGAGGGCGGCTCCCGAAGGCTGGGCCTGCTGGGCCTTCTCGAACCATGATGTCGT
TCGCCATATCAGTCGCTGGGCACATGGAGTCACCAGCCGTGATGCGCATGCGAAGCTGCTCGCCAGTCTGCTGATGTCGC
TGCGTGGAAGCGTCTGCATCTATCAGGGCGAGGAACTTGCGCTTGCCGAAGCGGAACTCGCCTATGAGGACCTCAAGGAT
CCCTATGGCATTCAGTTCTGGCCCGACTTCAAGGGCCGGGACGGCTGCCGTACGCCGATGGTCTGGGAGAGCCTCCCCGA
TGGCGGCTTCAGCAGCACGAGGCCTTGGCTGCCGATTTGCAAGAGTCACGTTCCGGGGGCAGTCGCCGTGCAGGAGAGCG
ATCCGAACTCCGTGCTGCATCACTACCGCCGCTTTCTCGCCTTCAGAAAAGCGCACCGGGCATTCGCCAAGGGAGAGATC
GAGTTCGTCGAGACCAAGGGGGCTCTGCTTGGCTTCCTTCGCATTCATGGCGATGAGAAGCTGTTCTGCCTGTTCAATAT
GAGCGACCAGCCAGCGACGAAGGAATTACCGATCAAGCGGCTTGAGCCGCTCGATGGTCACGGCTTCGTCTTTGAGATAA
TGGATCACGAGGTCAAGCTACCGGCCTGGGGCGCGTTTTTCGCGCGTCTGGCTTGA

Upstream 100 bases:

>100_bases
CTGATCGTCTTCTTGGCCCTGCAACGCTACCTCGTGCGCGGCCTGCTGGCTGGGTCGGTCAAGGGCGGCTGATCCATTCG
GACAAGGAAAACTGAACAGC

Downstream 100 bases:

>100_bases
GAACTGAGGGGAGGGTGCCATGACAGGCCTGCTGCTGAAAAATATCCGTAAGTCCTACGGGGCGGTCGACGTTATTCACG
GCATCAATCTCGAGATCAAG

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTLSETTSSLLEPDRDWWRGAVIYQIYPRSFQDTNGDGIGDLQGITARLPHIAGLGADAIWISPFFTSPMKDFGYDVSNY
VDVDPIFGALDDFDALIAEAHRLGLRVMIDLVLSHSSDQHPWFVESRSTRSNAKADWYVWADSKPDGTPPNNWLSIFGGS
AWQWDPTRLQYYLHNFLISQPDLNLHNSEVQDALLSVARFWLERGVDGFRLDTINFYFHDRELRDNPALAPERRNASTAP
AVNPYNYQEHIYDKNRPENLEFLQRFRALMDEFPAIAAVGEVGDSQRGLDIAAEYTSGGDKVHMCYAFEFLAPDQLTPQR
VAEVLRDFQRAAPEGWACWAFSNHDVVRHISRWAHGVTSRDAHAKLLASLLMSLRGSVCIYQGEELALAEAELAYEDLKD
PYGIQFWPDFKGRDGCRTPMVWESLPDGGFSSTRPWLPICKSHVPGAVAVQESDPNSVLHHYRRFLAFRKAHRAFAKGEI
EFVETKGALLGFLRIHGDEKLFCLFNMSDQPATKELPIKRLEPLDGHGFVFEIMDHEVKLPAWGAFFARLA

Sequences:

>Translated_551_residues
MTLSETTSSLLEPDRDWWRGAVIYQIYPRSFQDTNGDGIGDLQGITARLPHIAGLGADAIWISPFFTSPMKDFGYDVSNY
VDVDPIFGALDDFDALIAEAHRLGLRVMIDLVLSHSSDQHPWFVESRSTRSNAKADWYVWADSKPDGTPPNNWLSIFGGS
AWQWDPTRLQYYLHNFLISQPDLNLHNSEVQDALLSVARFWLERGVDGFRLDTINFYFHDRELRDNPALAPERRNASTAP
AVNPYNYQEHIYDKNRPENLEFLQRFRALMDEFPAIAAVGEVGDSQRGLDIAAEYTSGGDKVHMCYAFEFLAPDQLTPQR
VAEVLRDFQRAAPEGWACWAFSNHDVVRHISRWAHGVTSRDAHAKLLASLLMSLRGSVCIYQGEELALAEAELAYEDLKD
PYGIQFWPDFKGRDGCRTPMVWESLPDGGFSSTRPWLPICKSHVPGAVAVQESDPNSVLHHYRRFLAFRKAHRAFAKGEI
EFVETKGALLGFLRIHGDEKLFCLFNMSDQPATKELPIKRLEPLDGHGFVFEIMDHEVKLPAWGAFFARLA
>Mature_550_residues
TLSETTSSLLEPDRDWWRGAVIYQIYPRSFQDTNGDGIGDLQGITARLPHIAGLGADAIWISPFFTSPMKDFGYDVSNYV
DVDPIFGALDDFDALIAEAHRLGLRVMIDLVLSHSSDQHPWFVESRSTRSNAKADWYVWADSKPDGTPPNNWLSIFGGSA
WQWDPTRLQYYLHNFLISQPDLNLHNSEVQDALLSVARFWLERGVDGFRLDTINFYFHDRELRDNPALAPERRNASTAPA
VNPYNYQEHIYDKNRPENLEFLQRFRALMDEFPAIAAVGEVGDSQRGLDIAAEYTSGGDKVHMCYAFEFLAPDQLTPQRV
AEVLRDFQRAAPEGWACWAFSNHDVVRHISRWAHGVTSRDAHAKLLASLLMSLRGSVCIYQGEELALAEAELAYEDLKDP
YGIQFWPDFKGRDGCRTPMVWESLPDGGFSSTRPWLPICKSHVPGAVAVQESDPNSVLHHYRRFLAFRKAHRAFAKGEIE
FVETKGALLGFLRIHGDEKLFCLFNMSDQPATKELPIKRLEPLDGHGFVFEIMDHEVKLPAWGAFFARLA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=466, Percent_Identity=34.1201716738197, Blast_Score=271, Evalue=9e-73,
Organism=Escherichia coli, GI1790687, Length=522, Percent_Identity=33.5249042145594, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1786604, Length=525, Percent_Identity=22.0952380952381, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32565753, Length=476, Percent_Identity=25.2100840336134, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25147709, Length=494, Percent_Identity=24.6963562753036, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321726, Length=569, Percent_Identity=34.7978910369069, Blast_Score=290, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6322245, Length=553, Percent_Identity=34.9005424954792, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6321731, Length=567, Percent_Identity=34.0388007054674, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6319776, Length=567, Percent_Identity=34.2151675485009, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6324416, Length=570, Percent_Identity=34.5614035087719, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322241, Length=570, Percent_Identity=34.2105263157895, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6322021, Length=570, Percent_Identity=34.2105263157895, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24583747, Length=523, Percent_Identity=36.9024856596558, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24583749, Length=523, Percent_Identity=36.9024856596558, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24583745, Length=483, Percent_Identity=39.5445134575569, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24586593, Length=525, Percent_Identity=35.2380952380952, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24586597, Length=556, Percent_Identity=34.5323741007194, Blast_Score=301, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24586599, Length=498, Percent_Identity=33.9357429718875, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI221330053, Length=559, Percent_Identity=32.9159212880143, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24586587, Length=529, Percent_Identity=34.7826086956522, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI45549022, Length=525, Percent_Identity=33.3333333333333, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24586589, Length=514, Percent_Identity=35.0194552529183, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24586591, Length=510, Percent_Identity=33.5294117647059, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI281360393, Length=469, Percent_Identity=32.1961620469083, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 62542; Mature: 62411

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSETTSSLLEPDRDWWRGAVIYQIYPRSFQDTNGDGIGDLQGITARLPHIAGLGADAI
CCCCHHHHHHHCCCHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCEE
WISPFFTSPMKDFGYDVSNYVDVDPIFGALDDFDALIAEAHRLGLRVMIDLVLSHSSDQH
EECHHHCCCHHHHCCCHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWFVESRSTRSNAKADWYVWADSKPDGTPPNNWLSIFGGSAWQWDPTRLQYYLHNFLISQ
CEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCC
PDLNLHNSEVQDALLSVARFWLERGVDGFRLDTINFYFHDRELRDNPALAPERRNASTAP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
AVNPYNYQEHIYDKNRPENLEFLQRFRALMDEFPAIAAVGEVGDSQRGLDIAAEYTSGGD
CCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEHHCCCCCCCCCEEEEEECCCCC
KVHMCYAFEFLAPDQLTPQRVAEVLRDFQRAAPEGWACWAFSNHDVVRHISRWAHGVTSR
EEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCC
DAHAKLLASLLMSLRGSVCIYQGEELALAEAELAYEDLKDPYGIQFWPDFKGRDGCRTPM
HHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCC
VWESLPDGGFSSTRPWLPICKSHVPGAVAVQESDPNSVLHHYRRFLAFRKAHRAFAKGEI
HHHCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCE
EFVETKGALLGFLRIHGDEKLFCLFNMSDQPATKELPIKRLEPLDGHGFVFEIMDHEVKL
EEEECCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCC
PAWGAFFARLA
CHHHHHHHHCC
>Mature Secondary Structure 
TLSETTSSLLEPDRDWWRGAVIYQIYPRSFQDTNGDGIGDLQGITARLPHIAGLGADAI
CCCHHHHHHHCCCHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCEE
WISPFFTSPMKDFGYDVSNYVDVDPIFGALDDFDALIAEAHRLGLRVMIDLVLSHSSDQH
EECHHHCCCHHHHCCCHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWFVESRSTRSNAKADWYVWADSKPDGTPPNNWLSIFGGSAWQWDPTRLQYYLHNFLISQ
CEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCC
PDLNLHNSEVQDALLSVARFWLERGVDGFRLDTINFYFHDRELRDNPALAPERRNASTAP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
AVNPYNYQEHIYDKNRPENLEFLQRFRALMDEFPAIAAVGEVGDSQRGLDIAAEYTSGGD
CCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEHHCCCCCCCCCEEEEEECCCCC
KVHMCYAFEFLAPDQLTPQRVAEVLRDFQRAAPEGWACWAFSNHDVVRHISRWAHGVTSR
EEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCC
DAHAKLLASLLMSLRGSVCIYQGEELALAEAELAYEDLKDPYGIQFWPDFKGRDGCRTPM
HHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCC
VWESLPDGGFSSTRPWLPICKSHVPGAVAVQESDPNSVLHHYRRFLAFRKAHRAFAKGEI
HHHCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCE
EFVETKGALLGFLRIHGDEKLFCLFNMSDQPATKELPIKRLEPLDGHGFVFEIMDHEVKL
EEEECCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCC
PAWGAFFARLA
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]