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The map label for this gene is pncA [H]

Identifier: 150395426

GI number: 150395426

Start: 226557

End: 227156

Strand: Direct

Name: pncA [H]

Synonym: Smed_0199

Alternate gene names: 150395426

Gene position: 226557-227156 (Clockwise)

Preceding gene: 150395425

Following gene: 150395427

Centisome position: 5.99

GC content: 61.67

Gene sequence:

>600_bases
ATGGCTGACGAAGCCCTGATCGTCATCGATATGCAGAACGACTTCTGCCCGGGCGGCGCACTCGCAGTCGACGGCGGAGA
CGAAATCATTCCTGCCGTCAATCGCCTCATCGAGACGTCTCCGCATGTCGTGCTGACGCAGGACTGGCATCCGGCCGACC
ATTCGAGTTTCGCCTCCAGCCATCCTGGCAGGCTGGCGTTTCAAACCGTCACGATGCCCTATGGCGAGCAGACTCTCTGG
CCCGCGCATTGCGTTCAGGCAAGCGTCGGCGCCGATTTCCATCCATCGCTGCGATGGACCTCGGCGGAACTCGTCATCCG
CAAGGGTTTCCGCCGCGAGATCGATAGCTATTCGGCCTTTTTCGAGAACGATCACCGGACGCCGACGGGGCTTGCAGGCT
ATCTGCGCGAGCGTGGCATATCGAGCGTGACGCTTTGCGGCCTCGCCACGGATTTCTGCGTTGCCTTTTCGGCACTCGAT
GCGGTCGCACAAGGCTTTTCTACATCCGTGGTGCTGGACGCCTGCCGCGGCATAAATCTGAACGGCTCGCTGGCGGCCAT
GACGTTTCGCATGCGCGATGCCGGCGTCAGGCTGAGCTAG

Upstream 100 bases:

>100_bases
GCGTGCGCCGAACATGCTTGCCGCCGGGCGCATTCTATGGTTGGACTGGGTTTAAATCGATTTGTGGAAGGCCGGTGCGG
CTCGGGATTGCGAGGAGGCC

Downstream 100 bases:

>100_bases
CCCACCGGTGCGGGAAGCGCATATCCCGGCACCGCTTTACGAGATCGACCGCGATGGCCCGTTTCAAAGGGCTGTCATCA
CGGTTCAAGAGGAGCATGTC

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MADEALIVIDMQNDFCPGGALAVDGGDEIIPAVNRLIETSPHVVLTQDWHPADHSSFASSHPGRLAFQTVTMPYGEQTLW
PAHCVQASVGADFHPSLRWTSAELVIRKGFRREIDSYSAFFENDHRTPTGLAGYLRERGISSVTLCGLATDFCVAFSALD
AVAQGFSTSVVLDACRGINLNGSLAAMTFRMRDAGVRLS

Sequences:

>Translated_199_residues
MADEALIVIDMQNDFCPGGALAVDGGDEIIPAVNRLIETSPHVVLTQDWHPADHSSFASSHPGRLAFQTVTMPYGEQTLW
PAHCVQASVGADFHPSLRWTSAELVIRKGFRREIDSYSAFFENDHRTPTGLAGYLRERGISSVTLCGLATDFCVAFSALD
AVAQGFSTSVVLDACRGINLNGSLAAMTFRMRDAGVRLS
>Mature_198_residues
ADEALIVIDMQNDFCPGGALAVDGGDEIIPAVNRLIETSPHVVLTQDWHPADHSSFASSHPGRLAFQTVTMPYGEQTLWP
AHCVQASVGADFHPSLRWTSAELVIRKGFRREIDSYSAFFENDHRTPTGLAGYLRERGISSVTLCGLATDFCVAFSALDA
VAQGFSTSVVLDACRGINLNGSLAAMTFRMRDAGVRLS

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=209, Percent_Identity=44.4976076555024, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193206735, Length=212, Percent_Identity=36.7924528301887, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17543130, Length=213, Percent_Identity=36.150234741784, Blast_Score=119, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71999339, Length=221, Percent_Identity=33.4841628959276, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=191, Percent_Identity=32.9842931937173, Blast_Score=108, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648247, Length=200, Percent_Identity=35, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21503; Mature: 21372

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEALIVIDMQNDFCPGGALAVDGGDEIIPAVNRLIETSPHVVLTQDWHPADHSSFASS
CCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
HPGRLAFQTVTMPYGEQTLWPAHCVQASVGADFHPSLRWTSAELVIRKGFRREIDSYSAF
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHH
FENDHRTPTGLAGYLRERGISSVTLCGLATDFCVAFSALDAVAQGFSTSVVLDACRGINL
HCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
NGSLAAMTFRMRDAGVRLS
CCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
ADEALIVIDMQNDFCPGGALAVDGGDEIIPAVNRLIETSPHVVLTQDWHPADHSSFASS
CCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
HPGRLAFQTVTMPYGEQTLWPAHCVQASVGADFHPSLRWTSAELVIRKGFRREIDSYSAF
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHH
FENDHRTPTGLAGYLRERGISSVTLCGLATDFCVAFSALDAVAQGFSTSVVLDACRGINL
HCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
NGSLAAMTFRMRDAGVRLS
CCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]