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The map label for this gene is pncB [H]

Identifier: 150395427

GI number: 150395427

Start: 227261

End: 228565

Strand: Direct

Name: pncB [H]

Synonym: Smed_0200

Alternate gene names: 150395427

Gene position: 227261-228565 (Clockwise)

Preceding gene: 150395426

Following gene: 150395428

Centisome position: 6.01

GC content: 61.53

Gene sequence:

>1305_bases
ATGCCAAAGACCGATATCGCGCGGCGGGTCTATAACCACACCTGGAAGCTCGATCCGATTATTCGCAGTCTGCTGGACAC
GGATTTCTACAAGCTTCTGATGCTGCAGATGATATGGCAGCTTTATCCGGACGTCGACGCGACCTTTTCGCTGATCAACC
GTACGAAGACCGTCCGGCTTGCCGACGAGATAGACGAGCAGGAACTGCGGGACCAGCTCGACCACGCGCGTGGCCTGCGC
TTCACCAAGAAGGAAATGATCTGGCTAGCGGGTAACAGCTTCTATGGCCGCAAGCAGATATTTGCGCCCGAGTTCCTCGT
CTGGCTGGCGAAATTCCGTCTCCCGCCATACGAGCTGTCGCGCCGCGACGGTCAGTTCGAACTGACGTTTCGTGGCCGGT
GGGCCGAGACGACGATGTGGGAAATTCCTGCGCTCGCGATCATCAACGAGCTGCGCTCGCGCGCGGCGATGAAGGGGCTC
GGCCCCTTCACGCTGGACGTTCTCTATGCCCGGGCCAAGGCCAGGATGTGGTTGAAGGTCGAGCAGTTGCGGGAGCATCC
GAACCTTCGCATTTCCGACTTTGGAACGCGCCGGCGGCACAGCTTCCTGTGGCAGCGCTGGTGCGTCGAGGCGCTCAAGG
AAGGCATCGGCCCCTCCTTCACCGGGTCGAGCAACGTGCTTCTGGCGATGGACAACGATCTCGAGGCGGTCGGGACCAAT
GCGCATGAGCTGCCGATGGTGGCCGCCGCGCTCGCCCGCAACGACAGGGAGCTTGCGGCCGCACCCTACAAGGTTCTGCA
GGATTGGAATCAGCTCTACGGCGGCAATCTCCTGATCGTGTTGCCGGACGCGTTCGGGACGGCCGCATTCCTCCGGGATG
CGCCCGATTGGGTCGCCGACTGGACCGGTTTCCGGCCGGACAGCGCGCCGCCGATCGAGGGAGGCGAGAAGATCATCGCC
TGGTGGAAGAAGATGGGCCGGGATCCGCGCGAGAAGCTTCTGATCTTCTCCGACGGCCTCGACGTCGATACGATAGTCAA
GACCTACTGCCATTTCGAAGGGCGCGTGCGGATGAGCTTCGGTTGGGGAACCAATCTGACCAATGATTTCGCCGGCTGCG
CTCCAACCGAAATCAACGGTCTCAACCCGATCTCCGTCGTCTGCAAGGTCAGCGAAGCGAATGGCCACCCGGCCGTCAAG
CTCTCGGACAACCCGCGCAAGGCGACGGGGGATCCCGGCGAAGTCGAGCGCTATCTCAGATTTTTCGGCTCCGAGGACTT
TGTGGAGCAATCTGTACGGGTGTGA

Upstream 100 bases:

>100_bases
CCGGTGCGGGAAGCGCATATCCCGGCACCGCTTTACGAGATCGACCGCGATGGCCCGTTTCAAAGGGCTGTCATCACGGT
TCAAGAGGAGCATGTCGCAG

Downstream 100 bases:

>100_bases
TAAAAGCCGAAAAAACCGGCGGATGCGGATTCGAATTTGCGAATCATATGACTTATTGCGGTACCTCACTATTCCCTTGA
ATTTCCCCCCGGATACCCCA

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPKTDIARRVYNHTWKLDPIIRSLLDTDFYKLLMLQMIWQLYPDVDATFSLINRTKTVRLADEIDEQELRDQLDHARGLR
FTKKEMIWLAGNSFYGRKQIFAPEFLVWLAKFRLPPYELSRRDGQFELTFRGRWAETTMWEIPALAIINELRSRAAMKGL
GPFTLDVLYARAKARMWLKVEQLREHPNLRISDFGTRRRHSFLWQRWCVEALKEGIGPSFTGSSNVLLAMDNDLEAVGTN
AHELPMVAAALARNDRELAAAPYKVLQDWNQLYGGNLLIVLPDAFGTAAFLRDAPDWVADWTGFRPDSAPPIEGGEKIIA
WWKKMGRDPREKLLIFSDGLDVDTIVKTYCHFEGRVRMSFGWGTNLTNDFAGCAPTEINGLNPISVVCKVSEANGHPAVK
LSDNPRKATGDPGEVERYLRFFGSEDFVEQSVRV

Sequences:

>Translated_434_residues
MPKTDIARRVYNHTWKLDPIIRSLLDTDFYKLLMLQMIWQLYPDVDATFSLINRTKTVRLADEIDEQELRDQLDHARGLR
FTKKEMIWLAGNSFYGRKQIFAPEFLVWLAKFRLPPYELSRRDGQFELTFRGRWAETTMWEIPALAIINELRSRAAMKGL
GPFTLDVLYARAKARMWLKVEQLREHPNLRISDFGTRRRHSFLWQRWCVEALKEGIGPSFTGSSNVLLAMDNDLEAVGTN
AHELPMVAAALARNDRELAAAPYKVLQDWNQLYGGNLLIVLPDAFGTAAFLRDAPDWVADWTGFRPDSAPPIEGGEKIIA
WWKKMGRDPREKLLIFSDGLDVDTIVKTYCHFEGRVRMSFGWGTNLTNDFAGCAPTEINGLNPISVVCKVSEANGHPAVK
LSDNPRKATGDPGEVERYLRFFGSEDFVEQSVRV
>Mature_433_residues
PKTDIARRVYNHTWKLDPIIRSLLDTDFYKLLMLQMIWQLYPDVDATFSLINRTKTVRLADEIDEQELRDQLDHARGLRF
TKKEMIWLAGNSFYGRKQIFAPEFLVWLAKFRLPPYELSRRDGQFELTFRGRWAETTMWEIPALAIINELRSRAAMKGLG
PFTLDVLYARAKARMWLKVEQLREHPNLRISDFGTRRRHSFLWQRWCVEALKEGIGPSFTGSSNVLLAMDNDLEAVGTNA
HELPMVAAALARNDRELAAAPYKVLQDWNQLYGGNLLIVLPDAFGTAAFLRDAPDWVADWTGFRPDSAPPIEGGEKIIAW
WKKMGRDPREKLLIFSDGLDVDTIVKTYCHFEGRVRMSFGWGTNLTNDFAGCAPTEINGLNPISVVCKVSEANGHPAVKL
SDNPRKATGDPGEVERYLRFFGSEDFVEQSVRV

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family [H]

Homologues:

Organism=Escherichia coli, GI1787162, Length=407, Percent_Identity=34.3980343980344, Blast_Score=195, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6324783, Length=424, Percent_Identity=29.7169811320755, Blast_Score=167, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638 [H]

Pfam domain/function: PF04095 NAPRTase [H]

EC number: =2.4.2.11 [H]

Molecular weight: Translated: 49702; Mature: 49570

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTDIARRVYNHTWKLDPIIRSLLDTDFYKLLMLQMIWQLYPDVDATFSLINRTKTVRL
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEE
ADEIDEQELRDQLDHARGLRFTKKEMIWLAGNSFYGRKQIFAPEFLVWLAKFRLPPYELS
HHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHH
RRDGQFELTFRGRWAETTMWEIPALAIINELRSRAAMKGLGPFTLDVLYARAKARMWLKV
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EQLREHPNLRISDFGTRRRHSFLWQRWCVEALKEGIGPSFTGSSNVLLAMDNDLEAVGTN
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCCC
AHELPMVAAALARNDRELAAAPYKVLQDWNQLYGGNLLIVLPDAFGTAAFLRDAPDWVAD
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCHHHHH
WTGFRPDSAPPIEGGEKIIAWWKKMGRDPREKLLIFSDGLDVDTIVKTYCHFEGRVRMSF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEEEEEECCCCHHHHHHHHHCCCCEEEEEE
GWGTNLTNDFAGCAPTEINGLNPISVVCKVSEANGHPAVKLSDNPRKATGDPGEVERYLR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHH
FFGSEDFVEQSVRV
HHCCHHHHHHHCCC
>Mature Secondary Structure 
PKTDIARRVYNHTWKLDPIIRSLLDTDFYKLLMLQMIWQLYPDVDATFSLINRTKTVRL
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEE
ADEIDEQELRDQLDHARGLRFTKKEMIWLAGNSFYGRKQIFAPEFLVWLAKFRLPPYELS
HHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHH
RRDGQFELTFRGRWAETTMWEIPALAIINELRSRAAMKGLGPFTLDVLYARAKARMWLKV
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EQLREHPNLRISDFGTRRRHSFLWQRWCVEALKEGIGPSFTGSSNVLLAMDNDLEAVGTN
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCCC
AHELPMVAAALARNDRELAAAPYKVLQDWNQLYGGNLLIVLPDAFGTAAFLRDAPDWVAD
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCHHHHH
WTGFRPDSAPPIEGGEKIIAWWKKMGRDPREKLLIFSDGLDVDTIVKTYCHFEGRVRMSF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEEEEEECCCCHHHHHHHHHCCCCEEEEEE
GWGTNLTNDFAGCAPTEINGLNPISVVCKVSEANGHPAVKLSDNPRKATGDPGEVERYLR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHH
FFGSEDFVEQSVRV
HHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]