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The map label for this gene is phoB

Identifier: 150395352

GI number: 150395352

Start: 138486

End: 139169

Strand: Direct

Name: phoB

Synonym: Smed_0124

Alternate gene names: 150395352

Gene position: 138486-139169 (Clockwise)

Preceding gene: 150395351

Following gene: 150395355

Centisome position: 3.66

GC content: 64.91

Gene sequence:

>684_bases
ATGTTGCCGAAGATTGCCGTAGTCGAAGACGAGGAAGCCCTGAGCGTTCTCCTGCGCTATAATCTCGAGGCGGAAGGCTT
CGAGGTTGACACTATCCTTCGCGGCGACGAGGCGGAAATCCGCCTGCAGGAGCGCCTGCCCGACCTTCTCATTCTCGACT
GGATGCTGCCCGGGGTTTCCGGCATCGAGCTCTGCCGCAGGCTGCGCCAGCGGCCCGAGACGGAGCGCCTTCCGATCATC
ATGCTGACGGCGCGCGGCGAGGAAAGCGAGCGCGTGCGGGGCCTTGCGACCGGCGCCGACGACTATGTCGTCAAACCGTT
CTCGACGCCGGAACTGATGGCGAGGGTCAAGGCGATGCTCAGGCGAGCCAAGCCCGAGGTCCTGTCGACGCTCCTGCGCT
GCGGCGATATCGAGCTCGACCGGGAGACGCACCGGGTGCATCGCCGCAGCCGCGAAGTGCGCCTCGGCCCGACGGAGTTC
CGGCTGCTGGAATTCCTCATGTCCTCTCCGGGACGTGTCTTCTCCCGTTCCCAGCTTCTGGACGGTGTCTGGGGGCACGA
CATCTATGTCGACGAACGGACCGTCGACGTCCATGTCGGGCGTCTGCGCAAGGCGCTGAACTTCTCCAATATGCCGGATG
TCATCCGCACCGTGCGCGGCGCGGGCTACTCGTTGGAAAGCTGA

Upstream 100 bases:

>100_bases
ACGGACTGAGGTTCGGCCACGCGCCCCGCCTGAACTCATCGTGATCTGGACCGTGCGCGGCGCCATGCCGGCTGTCGTGC
GTAGGAAGGAGTCCTGCTGA

Downstream 100 bases:

>100_bases
GGCTGACGGCCGACGACGCAGGCACCGCTTGCGGAGGGGCTTTCCGGCCAGGGCTCGAACTGAGGTAGAATACTATCCGC
AGCAAACATCCGTCGCGAAG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MLPKIAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERLPDLLILDWMLPGVSGIELCRRLRQRPETERLPII
MLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAMLRRAKPEVLSTLLRCGDIELDRETHRVHRRSREVRLGPTEF
RLLEFLMSSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSNMPDVIRTVRGAGYSLES

Sequences:

>Translated_227_residues
MLPKIAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERLPDLLILDWMLPGVSGIELCRRLRQRPETERLPII
MLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAMLRRAKPEVLSTLLRCGDIELDRETHRVHRRSREVRLGPTEF
RLLEFLMSSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSNMPDVIRTVRGAGYSLES
>Mature_227_residues
MLPKIAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERLPDLLILDWMLPGVSGIELCRRLRQRPETERLPII
MLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAMLRRAKPEVLSTLLRCGDIELDRETHRVHRRSREVRLGPTEF
RLLEFLMSSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSNMPDVIRTVRGAGYSLES

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=47.5770925110132, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=44.2477876106195, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1788394, Length=230, Percent_Identity=40.4347826086956, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=38.5650224215247, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=38.3259911894273, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=37.0044052863436, Blast_Score=136, Evalue=9e-34,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.7713004484305, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=37.3873873873874, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=34.0807174887892, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=35.5855855855856, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=32.3529411764706, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI2367329, Length=239, Percent_Identity=35.9832635983264, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787229, Length=234, Percent_Identity=32.9059829059829, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=31.3304721030043, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=31.858407079646, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788550, Length=144, Percent_Identity=31.25, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHOB_RHIME (Q52990)

Other databases:

- EMBL:   M96261
- EMBL:   AL591688
- RefSeq:   NP_384621.1
- ProteinModelPortal:   Q52990
- SMR:   Q52990
- GeneID:   1232151
- GenomeReviews:   AL591688_GR
- KEGG:   sme:SMc02140
- NMPDR:   fig|266834.1.peg.1809
- HOGENOM:   HBG753323
- OMA:   RIAICNI
- ProtClustDB:   CLSK881438
- BioCyc:   SMEL266834:SMC02140-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862
- TIGRFAMs:   TIGR02154

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 26018; Mature: 26018

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKIAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERLPDLLILDWMLPGVS
CCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCHHHEEHHHHCCHHEEHHHHCCCCC
GIELCRRLRQRPETERLPIIMLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAML
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
RRAKPEVLSTLLRCGDIELDRETHRVHRRSREVRLGPTEFRLLEFLMSSPGRVFSRSQLL
HHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
DGVWGHDIYVDERTVDVHVGRLRKALNFSNMPDVIRTVRGAGYSLES
CCCCCCEEEECCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLPKIAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERLPDLLILDWMLPGVS
CCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCHHHEEHHHHCCHHEEHHHHCCCCC
GIELCRRLRQRPETERLPIIMLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAML
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
RRAKPEVLSTLLRCGDIELDRETHRVHRRSREVRLGPTEFRLLEFLMSSPGRVFSRSQLL
HHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
DGVWGHDIYVDERTVDVHVGRLRKALNFSNMPDVIRTVRGAGYSLES
CCCCCCEEEECCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430