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The map label for this gene is ada [H]

Identifier: 150395301

GI number: 150395301

Start: 81018

End: 82130

Strand: Direct

Name: ada [H]

Synonym: Smed_0073

Alternate gene names: 150395301

Gene position: 81018-82130 (Clockwise)

Preceding gene: 150395300

Following gene: 150395302

Centisome position: 2.14

GC content: 63.97

Gene sequence:

>1113_bases
ATGCTTTTCGATCTTCCGAATGATGATATCCTTTACGATGCGCTGCTGGCCCGCAGTTCCGATTATGAGGGCCAGGCCTT
CGTCTGCGTCAAAAGCACCGGCATCTTCTGCCGGCTCTCATGTCCCGCGCGCAAGCCGAAGCGGGAAAACAGCATTTTCT
TCGACAGTGTGTCCGCCTGCGTCAATTCCGGCTTCCGGCCGTGCCAACGCTGCCGACCGCTGGAGCAGGTGTCCGGTAAG
GATCCGCTGGTCAAAGACCTCCTGCGACTGCTCGACAGCCGTCCGGATCATCGCTGGACCGAGGGCGATCTCGTTCGGCG
CGGCTTCGATCCCTCCACCGTGCGGCGCGCCTTCAAGCGCAGCCTTGGCGTTACCTTTCTCGATCTCGCCCGGCAGCGGC
GAATGGGTGAGGCCGCGCGCCAGCTCTCCGTCGGCTCCAGCGTCATCGAGGCGCAGATCGACGCGGGCTACGAGTCTCCG
AGCGGTTTCCGCACCGCCTTCGCGCGGCTGGTCGGAGAGGCTCCTGCGAAATCGCAGGGCCGCGTGCTGCTGTTTGCCGA
CTGGGTAGAGACGCCGCTCGGTCCGATGGTGGCCGTTGCCGACCGGACGCATCTTCATCTGATCGAGTTTCATGACCGCC
GCGCGCTTCCCGCGGAAATGGAAAAGCTCAAGCGCAAGACGCTCTCGGCGGTGGTGCGCGGTAGAACGCCCCCCATCGAC
CAGGCGGAGCGTGAGCTCAAGAATTATTTTGCCGGTCGCTCTGCCGATTTTCGCACGCCCCTTGCGATGGACGGCAGTGC
TTTCGAGCGCCAGGTATGGGCAGAACTGGTGGCGATACCCGCAGGGGAGACGCGATCCTACAGCGACATCGCGCGGAAAA
TCGCCAGCCATCAGGCGGTCCGGGCCGTTGCCAGGGCCAATGGCGCCAACTGCTTTGCAATTCTCGTGCCTTGCCACCGC
TGCGTCGGTGCGGACGGATCTTTGACGGGCTATGGCGGTGGGCTGCAGCGCAAGCGATGGCTGCTGCGCCACGAGGGCAG
AATGCATCCTGCGGAGCTGTTCAAAGGTTGGGACGGGGAGGAGCCGGTGCAGGCCGGCGCTCTTTCGGTTTGA

Upstream 100 bases:

>100_bases
CGTGGTCATGCTGCTCGTGATATGAATCACCGGCGCGGTTGCACGAAATAGATGCCCGAATTTCGGCGGACGTCGCGCCT
GACGGATGCTACAAAGCAAT

Downstream 100 bases:

>100_bases
CAGGATCGATGAACGCATTGGATTGGGAGAAGACACGATGAGCCAGGAAGAAAATGCGCTTGCCTTCGAGGCACTGCACC
GGAAAGGCGATCCGGTGATT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MLFDLPNDDILYDALLARSSDYEGQAFVCVKSTGIFCRLSCPARKPKRENSIFFDSVSACVNSGFRPCQRCRPLEQVSGK
DPLVKDLLRLLDSRPDHRWTEGDLVRRGFDPSTVRRAFKRSLGVTFLDLARQRRMGEAARQLSVGSSVIEAQIDAGYESP
SGFRTAFARLVGEAPAKSQGRVLLFADWVETPLGPMVAVADRTHLHLIEFHDRRALPAEMEKLKRKTLSAVVRGRTPPID
QAERELKNYFAGRSADFRTPLAMDGSAFERQVWAELVAIPAGETRSYSDIARKIASHQAVRAVARANGANCFAILVPCHR
CVGADGSLTGYGGGLQRKRWLLRHEGRMHPAELFKGWDGEEPVQAGALSV

Sequences:

>Translated_370_residues
MLFDLPNDDILYDALLARSSDYEGQAFVCVKSTGIFCRLSCPARKPKRENSIFFDSVSACVNSGFRPCQRCRPLEQVSGK
DPLVKDLLRLLDSRPDHRWTEGDLVRRGFDPSTVRRAFKRSLGVTFLDLARQRRMGEAARQLSVGSSVIEAQIDAGYESP
SGFRTAFARLVGEAPAKSQGRVLLFADWVETPLGPMVAVADRTHLHLIEFHDRRALPAEMEKLKRKTLSAVVRGRTPPID
QAERELKNYFAGRSADFRTPLAMDGSAFERQVWAELVAIPAGETRSYSDIARKIASHQAVRAVARANGANCFAILVPCHR
CVGADGSLTGYGGGLQRKRWLLRHEGRMHPAELFKGWDGEEPVQAGALSV
>Mature_370_residues
MLFDLPNDDILYDALLARSSDYEGQAFVCVKSTGIFCRLSCPARKPKRENSIFFDSVSACVNSGFRPCQRCRPLEQVSGK
DPLVKDLLRLLDSRPDHRWTEGDLVRRGFDPSTVRRAFKRSLGVTFLDLARQRRMGEAARQLSVGSSVIEAQIDAGYESP
SGFRTAFARLVGEAPAKSQGRVLLFADWVETPLGPMVAVADRTHLHLIEFHDRRALPAEMEKLKRKTLSAVVRGRTPPID
QAERELKNYFAGRSADFRTPLAMDGSAFERQVWAELVAIPAGETRSYSDIARKIASHQAVRAVARANGANCFAILVPCHR
CVGADGSLTGYGGGLQRKRWLLRHEGRMHPAELFKGWDGEEPVQAGALSV

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=32.5301204819277, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788542, Length=354, Percent_Identity=34.4632768361582, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787596, Length=167, Percent_Identity=36.5269461077844, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=54.320987654321, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=54.320987654321, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=45.679012345679, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 41053; Mature: 41053

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFDLPNDDILYDALLARSSDYEGQAFVCVKSTGIFCRLSCPARKPKRENSIFFDSVSAC
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHH
VNSGFRPCQRCRPLEQVSGKDPLVKDLLRLLDSRPDHRWTEGDLVRRGFDPSTVRRAFKR
HCCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHH
SLGVTFLDLARQRRMGEAARQLSVGSSVIEAQIDAGYESPSGFRTAFARLVGEAPAKSQG
HHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
RVLLFADWVETPLGPMVAVADRTHLHLIEFHDRRALPAEMEKLKRKTLSAVVRGRTPPID
CEEEEECCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
QAERELKNYFAGRSADFRTPLAMDGSAFERQVWAELVAIPAGETRSYSDIARKIASHQAV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RAVARANGANCFAILVPCHRCVGADGSLTGYGGGLQRKRWLLRHEGRMHPAELFKGWDGE
HHHHHCCCCCEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCC
EPVQAGALSV
CCCCCCCCCC
>Mature Secondary Structure
MLFDLPNDDILYDALLARSSDYEGQAFVCVKSTGIFCRLSCPARKPKRENSIFFDSVSAC
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHH
VNSGFRPCQRCRPLEQVSGKDPLVKDLLRLLDSRPDHRWTEGDLVRRGFDPSTVRRAFKR
HCCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHH
SLGVTFLDLARQRRMGEAARQLSVGSSVIEAQIDAGYESPSGFRTAFARLVGEAPAKSQG
HHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
RVLLFADWVETPLGPMVAVADRTHLHLIEFHDRRALPAEMEKLKRKTLSAVVRGRTPPID
CEEEEECCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
QAERELKNYFAGRSADFRTPLAMDGSAFERQVWAELVAIPAGETRSYSDIARKIASHQAV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RAVARANGANCFAILVPCHRCVGADGSLTGYGGGLQRKRWLLRHEGRMHPAELFKGWDGE
HHHHHCCCCCEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCC
EPVQAGALSV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]