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The map label for this gene is ysgA [H]

Identifier: 150395253

GI number: 150395253

Start: 21029

End: 21889

Strand: Direct

Name: ysgA [H]

Synonym: Smed_0025

Alternate gene names: 150395253

Gene position: 21029-21889 (Clockwise)

Preceding gene: 150395252

Following gene: 150395254

Centisome position: 0.56

GC content: 62.95

Gene sequence:

>861_bases
ATGACCAACGACAGAAGCGACCATGGCACCCCACGCGTAGGGCAGGTGAAGGAGGTCACCAGCCTCACCAACCCGATCAT
CAAGGATATCCGGGCGCTGGCGCAGAAGAAGCATCGCGACGAGACGCGCTCCTTCATAGCCGAAGGACTGAAGCTCGTCA
TAGATGCGCTCGATCTCGGCTGGAGGATCAGGACGCTCGTCTATGCCAAGGCGGCGAAGGGCAAGCCTCAGGTCGAGCAG
GTGGCGGCGAAAACCGTGGCGAGAGGCGGGCTCGTCCTCGAGGTCAGCGAGAAGGTGATCTCCGCCATTACCCGGCGCGA
CAATCCGCAAATGGTTATCGGCATCTTCGAGCAGCGCTACCAGCCGCTCAAGGATATCCATCCGCGGGAGGGCGAGACCT
ATGTCGCGCTCGATCGGGTGCGCGATCCGGGCAATCTCGGCACGATCATTCGCACGGCCGACGCGGCCGGCGCTTCCGGA
ATCGTTCTTGTCGGCGAGACCACCGATCCCTTTTCGTTGGAGACAGTTCGGGCAACCATGGGTTCGGTCTTCGCCATGCC
GCTCGCACGGGCGAATGCGGAGGATTTCCTGCGTTGGCAGCGAGCAGCGGGCGTTCAGGTTGTGGCGACACATCTGGCTG
GCTCCGTCGACTATCGGACGATCGACTACAACTCGAAGCCTGTCGTGCTTCTGATGGGTAACGAGCAGGCGGGCCTGCCG
GTGGAACTGGCGCGGGAGGCCGGTGCACTCGCGCGCATCCCGCAGTCGGGACGTGCCGACTCGCTCAATCTCGCCATTGC
GACCGGTATCATGCTGTTCGAGGCACGGCGCCATCTTCTCTCGCTGGATGAGGGCCGATAA

Upstream 100 bases:

>100_bases
GGGTGGAATCGGGCGAACTCATCATTCGCGAGGGCGGTCTCGACGGCGCCAGGCCGGGCCGGGCGCTCTCGACCTCCCTC
TTCAGCCGCTGGGTACCGAA

Downstream 100 bases:

>100_bases
TGCAGGAACGAACGCTGTTCTCGCGGCCGCTCCCGATCGCGCTCTTCATCCTGACCGCGCTCGCCGCCGATCAGTTCATA
AAATATCTGGTCGAGGCGTT

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTNDRSDHGTPRVGQVKEVTSLTNPIIKDIRALAQKKHRDETRSFIAEGLKLVIDALDLGWRIRTLVYAKAAKGKPQVEQ
VAAKTVARGGLVLEVSEKVISAITRRDNPQMVIGIFEQRYQPLKDIHPREGETYVALDRVRDPGNLGTIIRTADAAGASG
IVLVGETTDPFSLETVRATMGSVFAMPLARANAEDFLRWQRAAGVQVVATHLAGSVDYRTIDYNSKPVVLLMGNEQAGLP
VELAREAGALARIPQSGRADSLNLAIATGIMLFEARRHLLSLDEGR

Sequences:

>Translated_286_residues
MTNDRSDHGTPRVGQVKEVTSLTNPIIKDIRALAQKKHRDETRSFIAEGLKLVIDALDLGWRIRTLVYAKAAKGKPQVEQ
VAAKTVARGGLVLEVSEKVISAITRRDNPQMVIGIFEQRYQPLKDIHPREGETYVALDRVRDPGNLGTIIRTADAAGASG
IVLVGETTDPFSLETVRATMGSVFAMPLARANAEDFLRWQRAAGVQVVATHLAGSVDYRTIDYNSKPVVLLMGNEQAGLP
VELAREAGALARIPQSGRADSLNLAIATGIMLFEARRHLLSLDEGR
>Mature_285_residues
TNDRSDHGTPRVGQVKEVTSLTNPIIKDIRALAQKKHRDETRSFIAEGLKLVIDALDLGWRIRTLVYAKAAKGKPQVEQV
AAKTVARGGLVLEVSEKVISAITRRDNPQMVIGIFEQRYQPLKDIHPREGETYVALDRVRDPGNLGTIIRTADAAGASGI
VLVGETTDPFSLETVRATMGSVFAMPLARANAEDFLRWQRAAGVQVVATHLAGSVDYRTIDYNSKPVVLLMGNEQAGLPV
ELAREAGALARIPQSGRADSLNLAIATGIMLFEARRHLLSLDEGR

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=33.7662337662338, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790623, Length=147, Percent_Identity=35.3741496598639, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24666840, Length=289, Percent_Identity=25.6055363321799, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 31165; Mature: 31034

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDRSDHGTPRVGQVKEVTSLTNPIIKDIRALAQKKHRDETRSFIAEGLKLVIDALDLG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WRIRTLVYAKAAKGKPQVEQVAAKTVARGGLVLEVSEKVISAITRRDNPQMVIGIFEQRY
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCEEEEEHHHCC
QPLKDIHPREGETYVALDRVRDPGNLGTIIRTADAAGASGIVLVGETTDPFSLETVRATM
CCHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
GSVFAMPLARANAEDFLRWQRAAGVQVVATHLAGSVDYRTIDYNSKPVVLLMGNEQAGLP
HHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCC
VELAREAGALARIPQSGRADSLNLAIATGIMLFEARRHLLSLDEGR
HHHHHHCCHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNDRSDHGTPRVGQVKEVTSLTNPIIKDIRALAQKKHRDETRSFIAEGLKLVIDALDLG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WRIRTLVYAKAAKGKPQVEQVAAKTVARGGLVLEVSEKVISAITRRDNPQMVIGIFEQRY
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCEEEEEHHHCC
QPLKDIHPREGETYVALDRVRDPGNLGTIIRTADAAGASGIVLVGETTDPFSLETVRATM
CCHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
GSVFAMPLARANAEDFLRWQRAAGVQVVATHLAGSVDYRTIDYNSKPVVLLMGNEQAGLP
HHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCC
VELAREAGALARIPQSGRADSLNLAIATGIMLFEARRHLLSLDEGR
HHHHHHCCHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]