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The map label for this gene is nifD [H]

Identifier: 150401853

GI number: 150401853

Start: 1485611

End: 1487038

Strand: Reverse

Name: nifD [H]

Synonym: Maeo_1431

Alternate gene names: 150401853

Gene position: 1487038-1485611 (Counterclockwise)

Preceding gene: 150401854

Following gene: 150401852

Centisome position: 94.75

GC content: 34.73

Gene sequence:

>1428_bases
ATGCCATATGTATTATTGGATTGCGATAACCCAATCCCCGAAAGAATGAAACATACCTATGTGTTTGACCCCGAAGAAAA
GATAATACCTGCATCCAATATAAACACAGTTCCAGGAGACATGACTGAAAGAGGTTGTGCCTTTGCAGGAGCTAGGGGTG
TAGTTGGAGGACCTGTTAAAGATATTATACACATGGTGCATGGTCCTGTGGGATGTGCATTTTATACCTGGGGATCAAGA
AGAGCTTTGTCAGACAATGAACTTCATAGAAGATACTGTTGGACAACAGATTTACAGGAATCAGACATTGTATATGGAGG
GGAAAAAACCCTTGAAAAAGCGTGTATGGAAGCAATTGACGAATTCCCAGAAGCTAATGGTATGATGATATATACCAACT
GTCCTACGGCACTTATTGGAGACAATATTGATGCAGTAGGTAATAAAGTTGAAGAATATATGAAAAAACCAGTATTTCCA
GTGAATTGTCCTGGATTTGCAGGAGTATCCCAGTCAAAAGGACACCATGTATTTAACACGATGTTTTACAGATGGTTAAA
GAAGAAAAAGGAACAATATCCTGAAAAATGTCTAAAAGAAGAAAATAAAACTCCATATGATATTGCATTAATCGGGGACT
ACAACATGGATTGGGATGTTGCTGTAATTAATGATTTATTTGAAAAAATTGGAGTAAGAAATGTAGTGACATTTACAGGA
AATGCTGCAATGGATGATTTGTTTAAATTAACAGATGTAAAATTAAATGTTGTTCACTGTCAAAGGTCTGCTGAATATAT
TGCTCACATGGTTCAAGATGGATTTAATGTGCCATTCACAAGGGTTTCTTTCTTTGGAATTACTAAAACTTCTGAAGCAT
TATATGATGTAGCTGAAAGATTGAATATACCAAAAGAAACAGTGGATGAAGTAATTGAAAAAGAATTAAAACCAATAAAC
GAAGAATTGGAATTCTATAAATCAAAACTTAACGGTAAAAAGTGCATGGTTTATGTTGGAGGTCCAAGAACCTGGCACTG
GATTATGGCTATGAAGGAATTGGGAATTGATTATGTGGTTGCATGCTGTACATTCTCCCATGAGGATGATTACGAAAAAA
TGAATATTAATCTTGAAAAAGCTGGATTAACTAATGTTCTTGTAATGGATTCACCAAATGAACCAGAATTGGAAGAGGCG
GCAGAAATATATAAACCTGATTTCATGCTTGTAGGATTAAAAGAAAGATATTTATTTAGAAAATTCGGAATTCCAACAGT
AAATTCACACAGTTATGAAGAAGGACCTTATGCAGGATACAGAGGATTTGTCAATTTTGCAAGAGATATTTATAAAGCGG
TTTGTCATCCAATTTGGAATACAATACATGAAAAAGAGGACAAATTTGAAGAATATAAGGTGAAATAA

Upstream 100 bases:

>100_bases
TAAGGAATTTAAATCTAGGTATTGCAATTATTGGTATTTAATTTAATTAATATTTGCAAATACCATTTAATTACTATAAT
TAATAATTTAAGGTGAAATA

Downstream 100 bases:

>100_bases
TGAGTGACCATACAGAATGTAGCAATTGCGGCGAAATAAACTACATGAAAAAAACAAGGAAAAGTACCATAAACCCAAAT
AAAATATGTCAGCCAATTGG

Product: nitrogenase component I, alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPYVLLDCDNPIPERMKHTYVFDPEEKIIPASNINTVPGDMTERGCAFAGARGVVGGPVKDIIHMVHGPVGCAFYTWGSR
RALSDNELHRRYCWTTDLQESDIVYGGEKTLEKACMEAIDEFPEANGMMIYTNCPTALIGDNIDAVGNKVEEYMKKPVFP
VNCPGFAGVSQSKGHHVFNTMFYRWLKKKKEQYPEKCLKEENKTPYDIALIGDYNMDWDVAVINDLFEKIGVRNVVTFTG
NAAMDDLFKLTDVKLNVVHCQRSAEYIAHMVQDGFNVPFTRVSFFGITKTSEALYDVAERLNIPKETVDEVIEKELKPIN
EELEFYKSKLNGKKCMVYVGGPRTWHWIMAMKELGIDYVVACCTFSHEDDYEKMNINLEKAGLTNVLVMDSPNEPELEEA
AEIYKPDFMLVGLKERYLFRKFGIPTVNSHSYEEGPYAGYRGFVNFARDIYKAVCHPIWNTIHEKEDKFEEYKVK

Sequences:

>Translated_475_residues
MPYVLLDCDNPIPERMKHTYVFDPEEKIIPASNINTVPGDMTERGCAFAGARGVVGGPVKDIIHMVHGPVGCAFYTWGSR
RALSDNELHRRYCWTTDLQESDIVYGGEKTLEKACMEAIDEFPEANGMMIYTNCPTALIGDNIDAVGNKVEEYMKKPVFP
VNCPGFAGVSQSKGHHVFNTMFYRWLKKKKEQYPEKCLKEENKTPYDIALIGDYNMDWDVAVINDLFEKIGVRNVVTFTG
NAAMDDLFKLTDVKLNVVHCQRSAEYIAHMVQDGFNVPFTRVSFFGITKTSEALYDVAERLNIPKETVDEVIEKELKPIN
EELEFYKSKLNGKKCMVYVGGPRTWHWIMAMKELGIDYVVACCTFSHEDDYEKMNINLEKAGLTNVLVMDSPNEPELEEA
AEIYKPDFMLVGLKERYLFRKFGIPTVNSHSYEEGPYAGYRGFVNFARDIYKAVCHPIWNTIHEKEDKFEEYKVK
>Mature_474_residues
PYVLLDCDNPIPERMKHTYVFDPEEKIIPASNINTVPGDMTERGCAFAGARGVVGGPVKDIIHMVHGPVGCAFYTWGSRR
ALSDNELHRRYCWTTDLQESDIVYGGEKTLEKACMEAIDEFPEANGMMIYTNCPTALIGDNIDAVGNKVEEYMKKPVFPV
NCPGFAGVSQSKGHHVFNTMFYRWLKKKKEQYPEKCLKEENKTPYDIALIGDYNMDWDVAVINDLFEKIGVRNVVTFTGN
AAMDDLFKLTDVKLNVVHCQRSAEYIAHMVQDGFNVPFTRVSFFGITKTSEALYDVAERLNIPKETVDEVIEKELKPINE
ELEFYKSKLNGKKCMVYVGGPRTWHWIMAMKELGIDYVVACCTFSHEDDYEKMNINLEKAGLTNVLVMDSPNEPELEEAA
EIYKPDFMLVGLKERYLFRKFGIPTVNSHSYEEGPYAGYRGFVNFARDIYKAVCHPIWNTIHEKEDKFEEYKVK

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR005974
- InterPro:   IPR010143
- InterPro:   IPR000318 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54352; Mature: 54220

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYVLLDCDNPIPERMKHTYVFDPEEKIIPASNINTVPGDMTERGCAFAGARGVVGGPVK
CCEEEEECCCCCHHHHCCCEEECCHHCCCCCCCCCCCCCCCHHCCCHHCCCCCCCCCCHH
DIIHMVHGPVGCAFYTWGSRRALSDNELHRRYCWTTDLQESDIVYGGEKTLEKACMEAID
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHH
EFPEANGMMIYTNCPTALIGDNIDAVGNKVEEYMKKPVFPVNCPGFAGVSQSKGHHVFNT
HCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
MFYRWLKKKKEQYPEKCLKEENKTPYDIALIGDYNMDWDVAVINDLFEKIGVRNVVTFTG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEC
NAAMDDLFKLTDVKLNVVHCQRSAEYIAHMVQDGFNVPFTRVSFFGITKTSEALYDVAER
CHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
LNIPKETVDEVIEKELKPINEELEFYKSKLNGKKCMVYVGGPRTWHWIMAMKELGIDYVV
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHEEE
ACCTFSHEDDYEKMNINLEKAGLTNVLVMDSPNEPELEEAAEIYKPDFMLVGLKERYLFR
HEEECCCCCCCHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEHHHHHHHH
KFGIPTVNSHSYEEGPYAGYRGFVNFARDIYKAVCHPIWNTIHEKEDKFEEYKVK
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PYVLLDCDNPIPERMKHTYVFDPEEKIIPASNINTVPGDMTERGCAFAGARGVVGGPVK
CEEEEECCCCCHHHHCCCEEECCHHCCCCCCCCCCCCCCCHHCCCHHCCCCCCCCCCHH
DIIHMVHGPVGCAFYTWGSRRALSDNELHRRYCWTTDLQESDIVYGGEKTLEKACMEAID
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHH
EFPEANGMMIYTNCPTALIGDNIDAVGNKVEEYMKKPVFPVNCPGFAGVSQSKGHHVFNT
HCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
MFYRWLKKKKEQYPEKCLKEENKTPYDIALIGDYNMDWDVAVINDLFEKIGVRNVVTFTG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEC
NAAMDDLFKLTDVKLNVVHCQRSAEYIAHMVQDGFNVPFTRVSFFGITKTSEALYDVAER
CHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
LNIPKETVDEVIEKELKPINEELEFYKSKLNGKKCMVYVGGPRTWHWIMAMKELGIDYVV
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHEEE
ACCTFSHEDDYEKMNINLEKAGLTNVLVMDSPNEPELEEAAEIYKPDFMLVGLKERYLFR
HEEECCCCCCCHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEHHHHHHHH
KFGIPTVNSHSYEEGPYAGYRGFVNFARDIYKAVCHPIWNTIHEKEDKFEEYKVK
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2503679 [H]