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The map label for this gene is nifK [H]

Identifier: 150401852

GI number: 150401852

Start: 1484193

End: 1485611

Strand: Reverse

Name: nifK [H]

Synonym: Maeo_1430

Alternate gene names: 150401852

Gene position: 1485611-1484193 (Counterclockwise)

Preceding gene: 150401853

Following gene: 150401851

Centisome position: 94.66

GC content: 36.58

Gene sequence:

>1419_bases
ATGAGTGACCATACAGAATGTAGCAATTGCGGCGAAATAAACTACATGAAAAAAACAAGGAAAAGTACCATAAACCCAAA
TAAAATATGTCAGCCAATTGGGGCAATGTGGGCAACATTGGGAGTTTGCGAAGGCGTCCCATTTGTTCAAGGTGGTCAAG
GTTGCACAACTTATGCAAGATATCAATTTGCAAGACATTTTAGAGAACCATCACCCATTGCATCTGCATCATTTCACGAA
CATGCTGCGGTATATGGGGGAATGAAAAACCTGGTTGATGGATTAACAAACCTTGTATCAAGATATGATCCAAACCTAAT
AGCCGTAATTACAACATGCTCCTCTGAAACAATTGGAGACGATATTGAATCATTTATTAGGGCTGCAAGAAAAAATATTG
CAAAGGAAGTTGGAGAAGAAAAAGCAGAATTACCAATTATTCCAGTTCACTGTCCAGCATATAAAGGAAGTCATGCAACA
GGATATGACAATGCTTCAAAAGCATATTTCCAATATTTGGCTAAAAAAGACCCAGAAGTTGAACCACATAAAATAAATAT
CATATCTGGATTTGGAACTAACCCCGGAGATATATTGGAAATAAAAAGAATGCTTAAAATGATGGGCTTAGAGGAAGGAA
AAGATTTCTCAATCATATTCGATATTAGTGAAAATCTTTACAAACCTTTGAGAGAACCTCTTGATGAAGTGCCATATTAC
CCAGACGCTGGAACTACCATTGAAGAATTTGTAGATGCTGGAAATGGTAAGGCAACTTTTGCATTATGTCAATCCGCAGG
AGGGGCTGGTGCCTTATTCTTAAAAAAGAAATTTAAAATACCTGATTTCTATGGACAACCAATTGGAGTTAAAAATACAG
ATGAATTTTTAATAAATGTAGCCCAACTTACAGGCAAAGGAATACCTGATGAGCTCCTTGATGAAAGAGGTAAATTAATA
GATGCTATTGCCGATACAGTCCACTATACAATGGATAAAAAGGTAGGTATATTCGGAGACCCTGACCATGTAATAGCTGT
GGCAAGATTCTGCTGTGAAATTGGTATGAAACCTGTTGTAGTTAGCACCCAAACACCTTCAAGAATATATAAAAAAGAAA
TGGAAAAAATTGCAAAAGAATTTAATGTGGATATTGAAGTTCAATTCTCTGACCTCTGGGACTTTGAAAAGAAAGTAAAA
GAAATAGGAGTAGATTTAATTATTGGACACCCAAGAGGGGGAACTGCAATAGCAAAAGATATGGGAATAGGACTTTTAAG
AACTGGATTCCCAGTATATGATAGAGTCGGATATTCCAGATGGCCAATAATGGGATACATGGGGAGTTTAAGATTCTTTG
ATGAGCTTGTAAATACTATATTAAGTACTAGTGTGCCAGAGACTCGACTCCAACAGTAA

Upstream 100 bases:

>100_bases
TTGTCAATTTTGCAAGAGATATTTATAAAGCGGTTTGTCATCCAATTTGGAATACAATACATGAAAAAGAGGACAAATTT
GAAGAATATAAGGTGAAATA

Downstream 100 bases:

>100_bases
GTTGAAAAATATAGATATAGTTAATCTTCGGACAATTTCATTACTATTCAATACACAAATCGTTATTTGTATTAACTAGG
GAAATTCGTTCAAAGTTTGG

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSDHTECSNCGEINYMKKTRKSTINPNKICQPIGAMWATLGVCEGVPFVQGGQGCTTYARYQFARHFREPSPIASASFHE
HAAVYGGMKNLVDGLTNLVSRYDPNLIAVITTCSSETIGDDIESFIRAARKNIAKEVGEEKAELPIIPVHCPAYKGSHAT
GYDNASKAYFQYLAKKDPEVEPHKINIISGFGTNPGDILEIKRMLKMMGLEEGKDFSIIFDISENLYKPLREPLDEVPYY
PDAGTTIEEFVDAGNGKATFALCQSAGGAGALFLKKKFKIPDFYGQPIGVKNTDEFLINVAQLTGKGIPDELLDERGKLI
DAIADTVHYTMDKKVGIFGDPDHVIAVARFCCEIGMKPVVVSTQTPSRIYKKEMEKIAKEFNVDIEVQFSDLWDFEKKVK
EIGVDLIIGHPRGGTAIAKDMGIGLLRTGFPVYDRVGYSRWPIMGYMGSLRFFDELVNTILSTSVPETRLQQ

Sequences:

>Translated_472_residues
MSDHTECSNCGEINYMKKTRKSTINPNKICQPIGAMWATLGVCEGVPFVQGGQGCTTYARYQFARHFREPSPIASASFHE
HAAVYGGMKNLVDGLTNLVSRYDPNLIAVITTCSSETIGDDIESFIRAARKNIAKEVGEEKAELPIIPVHCPAYKGSHAT
GYDNASKAYFQYLAKKDPEVEPHKINIISGFGTNPGDILEIKRMLKMMGLEEGKDFSIIFDISENLYKPLREPLDEVPYY
PDAGTTIEEFVDAGNGKATFALCQSAGGAGALFLKKKFKIPDFYGQPIGVKNTDEFLINVAQLTGKGIPDELLDERGKLI
DAIADTVHYTMDKKVGIFGDPDHVIAVARFCCEIGMKPVVVSTQTPSRIYKKEMEKIAKEFNVDIEVQFSDLWDFEKKVK
EIGVDLIIGHPRGGTAIAKDMGIGLLRTGFPVYDRVGYSRWPIMGYMGSLRFFDELVNTILSTSVPETRLQQ
>Mature_471_residues
SDHTECSNCGEINYMKKTRKSTINPNKICQPIGAMWATLGVCEGVPFVQGGQGCTTYARYQFARHFREPSPIASASFHEH
AAVYGGMKNLVDGLTNLVSRYDPNLIAVITTCSSETIGDDIESFIRAARKNIAKEVGEEKAELPIIPVHCPAYKGSHATG
YDNASKAYFQYLAKKDPEVEPHKINIISGFGTNPGDILEIKRMLKMMGLEEGKDFSIIFDISENLYKPLREPLDEVPYYP
DAGTTIEEFVDAGNGKATFALCQSAGGAGALFLKKKFKIPDFYGQPIGVKNTDEFLINVAQLTGKGIPDELLDERGKLID
AIADTVHYTMDKKVGIFGDPDHVIAVARFCCEIGMKPVVVSTQTPSRIYKKEMEKIAKEFNVDIEVQFSDLWDFEKKVKE
IGVDLIIGHPRGGTAIAKDMGIGLLRTGFPVYDRVGYSRWPIMGYMGSLRFFDELVNTILSTSVPETRLQQ

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 52260; Mature: 52129

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHTECSNCGEINYMKKTRKSTINPNKICQPIGAMWATLGVCEGVPFVQGGQGCTTYAR
CCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
YQFARHFREPSPIASASFHEHAAVYGGMKNLVDGLTNLVSRYDPNLIAVITTCSSETIGD
HHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
DIESFIRAARKNIAKEVGEEKAELPIIPVHCPAYKGSHATGYDNASKAYFQYLAKKDPEV
HHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
EPHKINIISGFGTNPGDILEIKRMLKMMGLEEGKDFSIIFDISENLYKPLREPLDEVPYY
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCC
PDAGTTIEEFVDAGNGKATFALCQSAGGAGALFLKKKFKIPDFYGQPIGVKNTDEFLINV
CCCCCHHHHHHHCCCCCEEEEEECCCCCCCEEEEEHCCCCCCCCCCCCCCCCHHHHHHHH
AQLTGKGIPDELLDERGKLIDAIADTVHYTMDKKVGIFGDPDHVIAVARFCCEIGMKPVV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
VSTQTPSRIYKKEMEKIAKEFNVDIEVQFSDLWDFEKKVKEIGVDLIIGHPRGGTAIAKD
EECCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
MGIGLLRTGFPVYDRVGYSRWPIMGYMGSLRFFDELVNTILSTSVPETRLQQ
HCCHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SDHTECSNCGEINYMKKTRKSTINPNKICQPIGAMWATLGVCEGVPFVQGGQGCTTYAR
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
YQFARHFREPSPIASASFHEHAAVYGGMKNLVDGLTNLVSRYDPNLIAVITTCSSETIGD
HHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
DIESFIRAARKNIAKEVGEEKAELPIIPVHCPAYKGSHATGYDNASKAYFQYLAKKDPEV
HHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
EPHKINIISGFGTNPGDILEIKRMLKMMGLEEGKDFSIIFDISENLYKPLREPLDEVPYY
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCC
PDAGTTIEEFVDAGNGKATFALCQSAGGAGALFLKKKFKIPDFYGQPIGVKNTDEFLINV
CCCCCHHHHHHHCCCCCEEEEEECCCCCCCEEEEEHCCCCCCCCCCCCCCCCHHHHHHHH
AQLTGKGIPDELLDERGKLIDAIADTVHYTMDKKVGIFGDPDHVIAVARFCCEIGMKPVV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
VSTQTPSRIYKKEMEKIAKEFNVDIEVQFSDLWDFEKKVKEIGVDLIIGHPRGGTAIAKD
EECCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
MGIGLLRTGFPVYDRVGYSRWPIMGYMGSLRFFDELVNTILSTSVPETRLQQ
HCCHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309 [H]