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The map label for this gene is nifK [H]

Identifier: 150401848

GI number: 150401848

Start: 1480457

End: 1481713

Strand: Reverse

Name: nifK [H]

Synonym: Maeo_1426

Alternate gene names: 150401848

Gene position: 1481713-1480457 (Counterclockwise)

Preceding gene: 150401849

Following gene: 150401847

Centisome position: 94.41

GC content: 30.63

Gene sequence:

>1257_bases
ATGAACCAGCTCATAGGTGGAGTTTTCGCCCTTATTGGCATATATAAATGTATTCCATTAGTGCATGGCTCCCAAGGATG
TGCAAATTATATAAAACATATAATTGTAGAACATTTTAAAGAGCCCACAGATATAGCTACAACTGGATTTCATGAATATT
CTGCAATACAGGGAGAAGGTATTGAAAATATCATTAAAGCAATTGAAAATATTGAAGTTAGTAAAAATCCTGAATTAATA
GGAATATGCACAACAGGACTTAGTGAAACCATTGGAGAAAATATAAAATTAGACATTAAAAACAACTACAACAACAAAAA
TATTGTGGCAATAAATACGCCATCATACAGTGGAAGTCATATTGATGGATACAACAATGCCATGTATAGTATAATCAGTG
GTATTTTAGAAAAAGATAATGAAATAGAGATTAAAAAAGAAAAAAATAATAAAATAAACCTAATTCACGGCGTAGTAAAT
CCCGGTGATGTTAGGGAGCTCCAACATATATTTAATAATCTTGGCATACAATATAATTTATTAAATGACATAAAATCAAT
GGATAGACCACTAAGACTTCCAAAAGAGGAATATCCTAAATGCGATACCACAATAGACAATATTAAAAATTCAATATACT
CAAAAGCTACCGTATCTTTTGGAATGGAAGGCGTAGATGGTGCATCATATTTGGAAAAATATGGGGTTATATCGTGTAAT
CTAAAATATCCAATTGGTATTAAAAATACAGACAAGTTTATATTAAATCTATTGGATACCGCAGGGGAAAATGATATATC
GGACATATCAAACATACCAGAACCGTTATTGGACGATAGGGGCTATGCCCTTGATGCTATGGCAAATACTAATGCCAAAT
TAAAAAATAAAAAAGTTGCAATATATGGAGCTCCCGACCAAGTTATAGGTCTTGTGGATTTGGCAAATGAAATTGGATTA
AAGGTTGTGGCAGTGCTATCCAATACAAAAACCAATTATTTTGCTGAAACCATACAAAAATTAAACCGAAATATTGAAGT
TATATCGGGAGATTTATATGATTTTGGAAAATCTTTAAAAGATAAAAATATAGACTTTATTATTGGAGACTATAGAAGTA
AATATATTGCAAATTCTTTAAATATTCCATCATTAAAAGTCGGATTCCCTGTAATTGATGTGTATGGACACCCTAAAAAA
GTCATGCTTGGATATAATGGTGCTTTAAATCTTATAAATGAAATGGCAAATTTATAG

Upstream 100 bases:

>100_bases
GATTATTATTAAAAAAATTAAACAATATTAATATTAATTAAAAAAATAAAAATGGAGGGATATTATCTCAAAAAAATCAT
TAACAGTCAATCCTCCATAT

Downstream 100 bases:

>100_bases
CCCCCACCATATTGAAGAAATATTAAATTTATTTTTTATTTTTGAATAGAGTCAAATTCCCAATCAAATCTATGATTTGC
GATTTTGAGACCCTCATTTT

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNQLIGGVFALIGIYKCIPLVHGSQGCANYIKHIIVEHFKEPTDIATTGFHEYSAIQGEGIENIIKAIENIEVSKNPELI
GICTTGLSETIGENIKLDIKNNYNNKNIVAINTPSYSGSHIDGYNNAMYSIISGILEKDNEIEIKKEKNNKINLIHGVVN
PGDVRELQHIFNNLGIQYNLLNDIKSMDRPLRLPKEEYPKCDTTIDNIKNSIYSKATVSFGMEGVDGASYLEKYGVISCN
LKYPIGIKNTDKFILNLLDTAGENDISDISNIPEPLLDDRGYALDAMANTNAKLKNKKVAIYGAPDQVIGLVDLANEIGL
KVVAVLSNTKTNYFAETIQKLNRNIEVISGDLYDFGKSLKDKNIDFIIGDYRSKYIANSLNIPSLKVGFPVIDVYGHPKK
VMLGYNGALNLINEMANL

Sequences:

>Translated_418_residues
MNQLIGGVFALIGIYKCIPLVHGSQGCANYIKHIIVEHFKEPTDIATTGFHEYSAIQGEGIENIIKAIENIEVSKNPELI
GICTTGLSETIGENIKLDIKNNYNNKNIVAINTPSYSGSHIDGYNNAMYSIISGILEKDNEIEIKKEKNNKINLIHGVVN
PGDVRELQHIFNNLGIQYNLLNDIKSMDRPLRLPKEEYPKCDTTIDNIKNSIYSKATVSFGMEGVDGASYLEKYGVISCN
LKYPIGIKNTDKFILNLLDTAGENDISDISNIPEPLLDDRGYALDAMANTNAKLKNKKVAIYGAPDQVIGLVDLANEIGL
KVVAVLSNTKTNYFAETIQKLNRNIEVISGDLYDFGKSLKDKNIDFIIGDYRSKYIANSLNIPSLKVGFPVIDVYGHPKK
VMLGYNGALNLINEMANL
>Mature_418_residues
MNQLIGGVFALIGIYKCIPLVHGSQGCANYIKHIIVEHFKEPTDIATTGFHEYSAIQGEGIENIIKAIENIEVSKNPELI
GICTTGLSETIGENIKLDIKNNYNNKNIVAINTPSYSGSHIDGYNNAMYSIISGILEKDNEIEIKKEKNNKINLIHGVVN
PGDVRELQHIFNNLGIQYNLLNDIKSMDRPLRLPKEEYPKCDTTIDNIKNSIYSKATVSFGMEGVDGASYLEKYGVISCN
LKYPIGIKNTDKFILNLLDTAGENDISDISNIPEPLLDDRGYALDAMANTNAKLKNKKVAIYGAPDQVIGLVDLANEIGL
KVVAVLSNTKTNYFAETIQKLNRNIEVISGDLYDFGKSLKDKNIDFIIGDYRSKYIANSLNIPSLKVGFPVIDVYGHPKK
VMLGYNGALNLINEMANL

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 46195; Mature: 46195

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLIGGVFALIGIYKCIPLVHGSQGCANYIKHIIVEHFKEPTDIATTGFHEYSAIQGEG
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCCCC
IENIIKAIENIEVSKNPELIGICTTGLSETIGENIKLDIKNNYNNKNIVAINTPSYSGSH
HHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCC
IDGYNNAMYSIISGILEKDNEIEIKKEKNNKINLIHGVVNPGDVRELQHIFNNLGIQYNL
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEH
LNDIKSMDRPLRLPKEEYPKCDTTIDNIKNSIYSKATVSFGMEGVDGASYLEKYGVISCN
HHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEE
LKYPIGIKNTDKFILNLLDTAGENDISDISNIPEPLLDDRGYALDAMANTNAKLKNKKVA
EECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCEEEEECCCCCEECCCEEE
IYGAPDQVIGLVDLANEIGLKVVAVLSNTKTNYFAETIQKLNRNIEVISGDLYDFGKSLK
EEECCHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHC
DKNIDFIIGDYRSKYIANSLNIPSLKVGFPVIDVYGHPKKVMLGYNGALNLINEMANL
CCCCCEEEECHHHHHHHCCCCCCEEEECCCEEEECCCCCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MNQLIGGVFALIGIYKCIPLVHGSQGCANYIKHIIVEHFKEPTDIATTGFHEYSAIQGEG
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCCCC
IENIIKAIENIEVSKNPELIGICTTGLSETIGENIKLDIKNNYNNKNIVAINTPSYSGSH
HHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCC
IDGYNNAMYSIISGILEKDNEIEIKKEKNNKINLIHGVVNPGDVRELQHIFNNLGIQYNL
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEH
LNDIKSMDRPLRLPKEEYPKCDTTIDNIKNSIYSKATVSFGMEGVDGASYLEKYGVISCN
HHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEE
LKYPIGIKNTDKFILNLLDTAGENDISDISNIPEPLLDDRGYALDAMANTNAKLKNKKVA
EECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCEEEEECCCCCEECCCEEE
IYGAPDQVIGLVDLANEIGLKVVAVLSNTKTNYFAETIQKLNRNIEVISGDLYDFGKSLK
EEECCHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHC
DKNIDFIIGDYRSKYIANSLNIPSLKVGFPVIDVYGHPKKVMLGYNGALNLINEMANL
CCCCCEEEECHHHHHHHCCCCCCEEEECCCEEEECCCCCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]