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The map label for this gene is cobQ

Identifier: 150401674

GI number: 150401674

Start: 1315085

End: 1316566

Strand: Reverse

Name: cobQ

Synonym: Maeo_1252

Alternate gene names: 150401674

Gene position: 1316566-1315085 (Counterclockwise)

Preceding gene: 150401676

Following gene: 150401672

Centisome position: 83.88

GC content: 30.09

Gene sequence:

>1482_bases
ATGGCAAAATTTATAATGGTAGTGGGAACATCCTCAAACAGTGGGAAAACAGTTTTAGTAGCTGGAATATGCAGAATACT
TGCAAACAAAGGTTATAAAGTAGCACCATTTAAATCCCAAAATATGAGTTTAAATTCGCGAGTAGCTAATGAGGACGGAG
AAATAGCCGTAGCTCAATACACCCAAAGTTTAGCTTCAAAGGTAGAACCATCCATTCATTTTAATCCAATATTATTAAAA
CCCATGGGAAATTTTGTATCTCAGGTTATTGTTCAGGGAGCTCCCTATAAAACAATAAATTATAATGAATATAGAGAGGA
TAAAGAATATTATTTAACTAAAATAAAGGAAAGTTTGGAATATTTAAACAAAAATTATGATTTCGTTGTTATGGAAGGGG
CAGGTAGTTGCTGTGAGATAAATCTATTGGGCGACGATATAGCCAACCTTAGAATTGCAGAACTATCAAATGCAGATGCC
ATATTAGTTTCAGATATTGATAGAGGCGGAGTATTTGCATCATTATATGGGACTGTGGAATTATTGCCCCAAAAATGGAA
AAAATTAATAAAAGGATTTGTAATAAATAAATTTAGAGGCAATGCCGAAGTATTAATTGACGGATACAAAAAAATTGAGG
AATTAACAAACATACCAGTTTTAGGAACTATACCATATAGTGAAGAGTTGGAGCTCCCGGAAGAGGATAGTCAGGCACTA
CAAAATAAAAAAGTATTTGGCAACATAAACAGCCCCGTTGAAGTAAATGTAGTAAGATTCTCAAAGATATCTAATTTTAC
AGATATTGATGCCTTTTCTAAAGATGCCCTTATTAAATTCATAGATTTTAAAGAGGATATAACTGGAGATATACTTATAC
TCCCAGGAACCAGATGTTCCACGGTTGAAATGGATTTAATGAAAAAATATAAAATGGATAAAAAAATAAAAGATTTTGCA
AAAAAAGGGGGCATCGTTATTGGAATATGTGGAGGATATCAAACAATGGGCAAAATGTTAATTGACGATACGCATTCGGA
AGGAGATATTGGAACAATTGAAGGAATTGGATTATTTGACATGGAAACTTATTTTGGAAATAAAAAGGCAATTGTAAATT
CAAATGGAATATTATCAATTAAAGATGATGATTTTTTAGTTAATGGTTATGAATTGCATGAGGGAATAACCCATTCAAAT
GAAAAACCACTTATAAAATTAAATAAAGGATTTGGCAATAAAAACGATGGATATGATGGTTCAATTAATATAAAAGATGG
AAATTATTTAATTGGAACATATTTCCACGGCATATTTGATAATTACGAGTTCAGAAATCATATTATAAATATGGTGTTGG
CAGAAAAAGGGCATAAATTAATAACCGAGGATAATTATAAAGATAATTTTGAAAAAAATATTAATAAATTTGCGGAAATT
ATTGAAAATAATGTAAATCTTGAAAAAATATTTAAAAAATAA

Upstream 100 bases:

>100_bases
GTCAGATATATAATTTTTGGCTTGTTGTAATATAATAAACTAAAATCTTTCTGACCCGTAAAGAACATACTTTCGAACAC
ACTATAAATTAGGTGGAACA

Downstream 100 bases:

>100_bases
CATTATAATAACTTTAATTTACCTGTTATTTTTTCCAGTTTTTCCTCTTTATCTGGACCTATTCCAACAGCCGTTGCTGT
CCCCGAAGGTATTTGGGTAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAKFIMVVGTSSNSGKTVLVAGICRILANKGYKVAPFKSQNMSLNSRVANEDGEIAVAQYTQSLASKVEPSIHFNPILLK
PMGNFVSQVIVQGAPYKTINYNEYREDKEYYLTKIKESLEYLNKNYDFVVMEGAGSCCEINLLGDDIANLRIAELSNADA
ILVSDIDRGGVFASLYGTVELLPQKWKKLIKGFVINKFRGNAEVLIDGYKKIEELTNIPVLGTIPYSEELELPEEDSQAL
QNKKVFGNINSPVEVNVVRFSKISNFTDIDAFSKDALIKFIDFKEDITGDILILPGTRCSTVEMDLMKKYKMDKKIKDFA
KKGGIVIGICGGYQTMGKMLIDDTHSEGDIGTIEGIGLFDMETYFGNKKAIVNSNGILSIKDDDFLVNGYELHEGITHSN
EKPLIKLNKGFGNKNDGYDGSINIKDGNYLIGTYFHGIFDNYEFRNHIINMVLAEKGHKLITEDNYKDNFEKNINKFAEI
IENNVNLEKIFKK

Sequences:

>Translated_493_residues
MAKFIMVVGTSSNSGKTVLVAGICRILANKGYKVAPFKSQNMSLNSRVANEDGEIAVAQYTQSLASKVEPSIHFNPILLK
PMGNFVSQVIVQGAPYKTINYNEYREDKEYYLTKIKESLEYLNKNYDFVVMEGAGSCCEINLLGDDIANLRIAELSNADA
ILVSDIDRGGVFASLYGTVELLPQKWKKLIKGFVINKFRGNAEVLIDGYKKIEELTNIPVLGTIPYSEELELPEEDSQAL
QNKKVFGNINSPVEVNVVRFSKISNFTDIDAFSKDALIKFIDFKEDITGDILILPGTRCSTVEMDLMKKYKMDKKIKDFA
KKGGIVIGICGGYQTMGKMLIDDTHSEGDIGTIEGIGLFDMETYFGNKKAIVNSNGILSIKDDDFLVNGYELHEGITHSN
EKPLIKLNKGFGNKNDGYDGSINIKDGNYLIGTYFHGIFDNYEFRNHIINMVLAEKGHKLITEDNYKDNFEKNINKFAEI
IENNVNLEKIFKK
>Mature_492_residues
AKFIMVVGTSSNSGKTVLVAGICRILANKGYKVAPFKSQNMSLNSRVANEDGEIAVAQYTQSLASKVEPSIHFNPILLKP
MGNFVSQVIVQGAPYKTINYNEYREDKEYYLTKIKESLEYLNKNYDFVVMEGAGSCCEINLLGDDIANLRIAELSNADAI
LVSDIDRGGVFASLYGTVELLPQKWKKLIKGFVINKFRGNAEVLIDGYKKIEELTNIPVLGTIPYSEELELPEEDSQALQ
NKKVFGNINSPVEVNVVRFSKISNFTDIDAFSKDALIKFIDFKEDITGDILILPGTRCSTVEMDLMKKYKMDKKIKDFAK
KGGIVIGICGGYQTMGKMLIDDTHSEGDIGTIEGIGLFDMETYFGNKKAIVNSNGILSIKDDDFLVNGYELHEGITHSNE
KPLIKLNKGFGNKNDGYDGSINIKDGNYLIGTYFHGIFDNYEFRNHIINMVLAEKGHKLITEDNYKDNFEKNINKFAEII
ENNVNLEKIFKK

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_META3 (A6UWF6)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325440.1
- ProteinModelPortal:   A6UWF6
- STRING:   A6UWF6
- GeneID:   5327060
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1252
- eggNOG:   arNOG04142
- HOGENOM:   HBG302271
- OMA:   ICLPRIS
- ProtClustDB:   PRK00784
- BioCyc:   MAEO419665:MAEO_1252-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 55192; Mature: 55061

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 330-330 ACT_SITE 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFIMVVGTSSNSGKTVLVAGICRILANKGYKVAPFKSQNMSLNSRVANEDGEIAVAQY
CCEEEEEEECCCCCCCEEHHHHHHHHHHCCCEEECCCCCCCCCCCCEECCCCCCEEHHHH
TQSLASKVEPSIHFNPILLKPMGNFVSQVIVQGAPYKTINYNEYREDKEYYLTKIKESLE
HHHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCCCEECCCCHHHCHHHHHHHHHHHHHH
YLNKNYDFVVMEGAGSCCEINLLGDDIANLRIAELSNADAILVSDIDRGGVFASLYGTVE
HHCCCCCEEEECCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCEEEEHHHHHH
LLPQKWKKLIKGFVINKFRGNAEVLIDGYKKIEELTNIPVLGTIPYSEELELPEEDSQAL
HHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
QNKKVFGNINSPVEVNVVRFSKISNFTDIDAFSKDALIKFIDFKEDITGDILILPGTRCS
HCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCC
TVEMDLMKKYKMDKKIKDFAKKGGIVIGICGGYQTMGKMLIDDTHSEGDIGTIEGIGLFD
EEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHEECCCCCCCCEEECCCEEEE
METYFGNKKAIVNSNGILSIKDDDFLVNGYELHEGITHSNEKPLIKLNKGFGNKNDGYDG
EHHHCCCCEEEEECCCEEEECCCCEEEECEEHHCCCCCCCCCCEEEEECCCCCCCCCCCC
SINIKDGNYLIGTYFHGIFDNYEFRNHIINMVLAEKGHKLITEDNYKDNFEKNINKFAEI
EEEECCCCEEEEEEHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
IENNVNLEKIFKK
HHCCCCHHHHHCC
>Mature Secondary Structure 
AKFIMVVGTSSNSGKTVLVAGICRILANKGYKVAPFKSQNMSLNSRVANEDGEIAVAQY
CEEEEEEECCCCCCCEEHHHHHHHHHHCCCEEECCCCCCCCCCCCEECCCCCCEEHHHH
TQSLASKVEPSIHFNPILLKPMGNFVSQVIVQGAPYKTINYNEYREDKEYYLTKIKESLE
HHHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCCCEECCCCHHHCHHHHHHHHHHHHHH
YLNKNYDFVVMEGAGSCCEINLLGDDIANLRIAELSNADAILVSDIDRGGVFASLYGTVE
HHCCCCCEEEECCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCEEEEHHHHHH
LLPQKWKKLIKGFVINKFRGNAEVLIDGYKKIEELTNIPVLGTIPYSEELELPEEDSQAL
HHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
QNKKVFGNINSPVEVNVVRFSKISNFTDIDAFSKDALIKFIDFKEDITGDILILPGTRCS
HCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCC
TVEMDLMKKYKMDKKIKDFAKKGGIVIGICGGYQTMGKMLIDDTHSEGDIGTIEGIGLFD
EEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHEECCCCCCCCEEECCCEEEE
METYFGNKKAIVNSNGILSIKDDDFLVNGYELHEGITHSNEKPLIKLNKGFGNKNDGYDG
EHHHCCCCEEEEECCCEEEECCCCEEEECEEHHCCCCCCCCCCEEEEECCCCCCCCCCCC
SINIKDGNYLIGTYFHGIFDNYEFRNHIINMVLAEKGHKLITEDNYKDNFEKNINKFAEI
EEEECCCCEEEEEEHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
IENNVNLEKIFKK
HHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA