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The map label for this gene is frhA [H]

Identifier: 150401469

GI number: 150401469

Start: 1077463

End: 1078620

Strand: Reverse

Name: frhA [H]

Synonym: Maeo_1045

Alternate gene names: 150401469

Gene position: 1078620-1077463 (Counterclockwise)

Preceding gene: 150401470

Following gene: 150401468

Centisome position: 68.72

GC content: 37.91

Gene sequence:

>1158_bases
GTGCCAGAAGAAATTAGAATTACCCCTACCACAAGACATGAGGGGCATGCTAAATTAATATTAGAAGTGAATGACGAAGG
ATCAGTAAGTAAAGCATATTACCCAAATACTACTCCCGTTAGGGGATTTGAGACAATGTTAAAGGGCAAACCTGCAAGTT
TTGCACCTATTGCCGTTATGAGAATTTGCGGTATTTGTCAAACCACCCACGGTATAGCATCATGCGAGGCTGTTGAAGAT
GCAATTGGATGCGAAATACCAGAAGACGGTAAATTACTAAGAGAATTGGTGGGATTAGGAAACAGAATGCACTCCCACCC
ATTACATCATCTATTGACTGCTGACGACTTTATAAAACCAGAGGAAACTGAATTAAAAACAAGTGCTATTACCCTTATTC
AAAGAATGAGGAAAGTAGGGCAGTTAATAGTAGATGTCGTAGGTGGGGAAGGAATACACCCACCAAACATAGTAATTGGT
GGTATGAGAACCAATATTTCAGAAAGAGCAAAATCAAAATTATATTATGCTTGTAAACAATATGAAAAAGATGCCTATGA
AATGTATGATGTATATACTATGTTAATCGAGAGATACCTCGAAGAAATCGGAATACCTGATTTAGGGGCTCACGACTTCC
CATACTTAGCAACACATACAACATATGGAGATAAGGATGCTATAAGTTGGAATGATGTTACAGAAATACCTGCCCAAAGA
TACTATAAAAATAAAGAAGTAGCCCAAACATCAACAAGTCAAATACCACTTTATGGAGGAGTTCCAGTAGAAGGAGGTCC
AAGAGCAAGAATGGTTAAATTTGGAGATTTAAAAGCTGGTGGCGGAGTAATGGATATAAACATTGCAAGAGCTATGGAAA
ACTTAGGAGCAGCATACAGGGCATTAGATATACTTGACCAACTAAATATTAATGGAAGCACAAAAGTAGTTCCAGAATAT
AAGGACGGTTCTGGAATTGGTGTTCATGAAGCTCCAAGAGCTACAAATACCCACATGGCAGAAGTTGGAAAAGACGGAAA
AATTAAAAATTACAACATTATTGCAGCTTCAACATGGAACTTCCCAGTAGTTGAAAAGGCAATTGAAAAATACCCGCACG
AATATGCAGAAGTCATAATGCGTGCCTATGATATATGA

Upstream 100 bases:

>100_bases
TGTGATTTAAAAAACTAATGTCCCCCATTTACATATTTAAAAGATATTTATTTTATATTGTGCTATTGAAATATATATTA
TTCATAAGGAGGTGAATATT

Downstream 100 bases:

>100_bases
GCTTCATGTGCTACGCACATCATCGTAAAAGATGACCAGACAAAAGAATTAATTGAAGTAAGAAAAATATAAGATATTAA
AAAGATGCGAGGGGAACTCG

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha; FRH [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MPEEIRITPTTRHEGHAKLILEVNDEGSVSKAYYPNTTPVRGFETMLKGKPASFAPIAVMRICGICQTTHGIASCEAVED
AIGCEIPEDGKLLRELVGLGNRMHSHPLHHLLTADDFIKPEETELKTSAITLIQRMRKVGQLIVDVVGGEGIHPPNIVIG
GMRTNISERAKSKLYYACKQYEKDAYEMYDVYTMLIERYLEEIGIPDLGAHDFPYLATHTTYGDKDAISWNDVTEIPAQR
YYKNKEVAQTSTSQIPLYGGVPVEGGPRARMVKFGDLKAGGGVMDINIARAMENLGAAYRALDILDQLNINGSTKVVPEY
KDGSGIGVHEAPRATNTHMAEVGKDGKIKNYNIIAASTWNFPVVEKAIEKYPHEYAEVIMRAYDI

Sequences:

>Translated_385_residues
MPEEIRITPTTRHEGHAKLILEVNDEGSVSKAYYPNTTPVRGFETMLKGKPASFAPIAVMRICGICQTTHGIASCEAVED
AIGCEIPEDGKLLRELVGLGNRMHSHPLHHLLTADDFIKPEETELKTSAITLIQRMRKVGQLIVDVVGGEGIHPPNIVIG
GMRTNISERAKSKLYYACKQYEKDAYEMYDVYTMLIERYLEEIGIPDLGAHDFPYLATHTTYGDKDAISWNDVTEIPAQR
YYKNKEVAQTSTSQIPLYGGVPVEGGPRARMVKFGDLKAGGGVMDINIARAMENLGAAYRALDILDQLNINGSTKVVPEY
KDGSGIGVHEAPRATNTHMAEVGKDGKIKNYNIIAASTWNFPVVEKAIEKYPHEYAEVIMRAYDI
>Mature_384_residues
PEEIRITPTTRHEGHAKLILEVNDEGSVSKAYYPNTTPVRGFETMLKGKPASFAPIAVMRICGICQTTHGIASCEAVEDA
IGCEIPEDGKLLRELVGLGNRMHSHPLHHLLTADDFIKPEETELKTSAITLIQRMRKVGQLIVDVVGGEGIHPPNIVIGG
MRTNISERAKSKLYYACKQYEKDAYEMYDVYTMLIERYLEEIGIPDLGAHDFPYLATHTTYGDKDAISWNDVTEIPAQRY
YKNKEVAQTSTSQIPLYGGVPVEGGPRARMVKFGDLKAGGGVMDINIARAMENLGAAYRALDILDQLNINGSTKVVPEYK
DGSGIGVHEAPRATNTHMAEVGKDGKIKNYNIIAASTWNFPVVEKAIEKYPHEYAEVIMRAYDI

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=119, Percent_Identity=35.2941176470588, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787207, Length=113, Percent_Identity=34.5132743362832, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017682
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 42611; Mature: 42480

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEEIRITPTTRHEGHAKLILEVNDEGSVSKAYYPNTTPVRGFETMLKGKPASFAPIAVM
CCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHH
RICGICQTTHGIASCEAVEDAIGCEIPEDGKLLRELVGLGNRMHSHPLHHLLTADDFIKP
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCC
EETELKTSAITLIQRMRKVGQLIVDVVGGEGIHPPNIVIGGMRTNISERAKSKLYYACKQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHCCHHHHHHHHHHHHHHH
YEKDAYEMYDVYTMLIERYLEEIGIPDLGAHDFPYLATHTTYGDKDAISWNDVTEIPAQR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCHHH
YYKNKEVAQTSTSQIPLYGGVPVEGGPRARMVKFGDLKAGGGVMDINIARAMENLGAAYR
HHCCCHHHHCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHH
ALDILDQLNINGSTKVVPEYKDGSGIGVHEAPRATNTHMAEVGKDGKIKNYNIIAASTWN
HHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCC
FPVVEKAIEKYPHEYAEVIMRAYDI
CHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PEEIRITPTTRHEGHAKLILEVNDEGSVSKAYYPNTTPVRGFETMLKGKPASFAPIAVM
CCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHH
RICGICQTTHGIASCEAVEDAIGCEIPEDGKLLRELVGLGNRMHSHPLHHLLTADDFIKP
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCC
EETELKTSAITLIQRMRKVGQLIVDVVGGEGIHPPNIVIGGMRTNISERAKSKLYYACKQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHCCHHHHHHHHHHHHHHH
YEKDAYEMYDVYTMLIERYLEEIGIPDLGAHDFPYLATHTTYGDKDAISWNDVTEIPAQR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCHHH
YYKNKEVAQTSTSQIPLYGGVPVEGGPRARMVKFGDLKAGGGVMDINIARAMENLGAAYR
HHCCCHHHHCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHH
ALDILDQLNINGSTKVVPEYKDGSGIGVHEAPRATNTHMAEVGKDGKIKNYNIIAASTWN
HHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCC
FPVVEKAIEKYPHEYAEVIMRAYDI
CHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]