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The map label for this gene is bioB [C]

Identifier: 150401450

GI number: 150401450

Start: 1054554

End: 1055588

Strand: Direct

Name: bioB [C]

Synonym: Maeo_1026

Alternate gene names: 150401450

Gene position: 1054554-1055588 (Clockwise)

Preceding gene: 150401449

Following gene: 150401451

Centisome position: 67.19

GC content: 31.5

Gene sequence:

>1035_bases
ATGGATTTTTCACACATACGGGACAATTTTGAAAATTTAAAAGAAGATACTTCCAAAGAAAACCTAAAAAACGGACTAAT
ATCAAAAGAAGATGCCTTAAAGTTATTTAATATTTCCAAAGGTAATGATTATTTAAATTTATTCAGTATTGCATCAGAAG
TAAGGGACTATTTTAAAAAAGAAATAGAAATAATATCTTCAATCCATATCACCAATATATGTTCCGTAAATCCGAAATGT
TATTATTGTGGTTTCGCGGCGGGAACTTCAAAGGAAGGATATTATGAGCCATTTAGAACGACAGACGAAGAAATAAAAAA
TTCCGCAATAGCAATTGAAAAAAGTGGAATTATGAGAGTTAGCTGTTCCTCAGCACATGGATATGGGGGCAAAGAAACAT
TACGAACTTTGAAAATAGTTAAAGAAAATACAAATTTAGAAGTTTTAGTAAATGCTGGGGCAGATTTTACAGAAAACTGC
ATAAAAGAATTAAAAAAACATGACATCGATATAATTTGCTGTAATTTAGAAACCATTAATAATAAAGTATTTAAATCAGT
TAAACCAGGGGAAGATTTAGAAAATAGAATAAATATCTGTAAATTGGTAAAAAAATATGATATAAATTTATCATCTGGTT
TATTGTTAGGAATAGGGGAAAGTTATGAGGATAGGGCAAATCATTTATTGTTTTTAAAGGAGCTCCCTGTGGATGAAATT
CCTATAATGGGATTTAATCCATATAAAGATACACCGATGGAAAACCATCCAAAATGTTCGGCGTTGGAACAGGCTAAAAC
TATTGCCATAAGTAGGTTGATGTTTCCAGATATTAGAATTACATCACCAACCCCAACAATTGGAGCGGAGCTTGTTCAAT
TTGCACTAATGGGAGGGGCAAGCAACATAGCAACCGTAATACCAGACAACCACCCAATGAATATAAAAGGAGTGGGAAAT
CCAAAAACGGGCAATTTAAAAGAAGTTGTTAAAATGATTGAAGAGTTAGGACTAACTCCAAAAATAAAAAAATAA

Upstream 100 bases:

>100_bases
CATTTTTATGTCATAAGAATAATTTATTTTTAAATTAAATATATTAAATTTATAAACCTCCACAGGCAACCAATATAATA
TATAATAAATCGGTGATTTA

Downstream 100 bases:

>100_bases
ATTGGGGCATTATGAAAAATCTAATCGGAATTAACCCAAATCAAAGATTGGTGGCAATAACCAGGATGTATAGGGATATT
GCAGAAATAATCGATATAGA

Product: biotin synthase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDFSHIRDNFENLKEDTSKENLKNGLISKEDALKLFNISKGNDYLNLFSIASEVRDYFKKEIEIISSIHITNICSVNPKC
YYCGFAAGTSKEGYYEPFRTTDEEIKNSAIAIEKSGIMRVSCSSAHGYGGKETLRTLKIVKENTNLEVLVNAGADFTENC
IKELKKHDIDIICCNLETINNKVFKSVKPGEDLENRINICKLVKKYDINLSSGLLLGIGESYEDRANHLLFLKELPVDEI
PIMGFNPYKDTPMENHPKCSALEQAKTIAISRLMFPDIRITSPTPTIGAELVQFALMGGASNIATVIPDNHPMNIKGVGN
PKTGNLKEVVKMIEELGLTPKIKK

Sequences:

>Translated_344_residues
MDFSHIRDNFENLKEDTSKENLKNGLISKEDALKLFNISKGNDYLNLFSIASEVRDYFKKEIEIISSIHITNICSVNPKC
YYCGFAAGTSKEGYYEPFRTTDEEIKNSAIAIEKSGIMRVSCSSAHGYGGKETLRTLKIVKENTNLEVLVNAGADFTENC
IKELKKHDIDIICCNLETINNKVFKSVKPGEDLENRINICKLVKKYDINLSSGLLLGIGESYEDRANHLLFLKELPVDEI
PIMGFNPYKDTPMENHPKCSALEQAKTIAISRLMFPDIRITSPTPTIGAELVQFALMGGASNIATVIPDNHPMNIKGVGN
PKTGNLKEVVKMIEELGLTPKIKK
>Mature_344_residues
MDFSHIRDNFENLKEDTSKENLKNGLISKEDALKLFNISKGNDYLNLFSIASEVRDYFKKEIEIISSIHITNICSVNPKC
YYCGFAAGTSKEGYYEPFRTTDEEIKNSAIAIEKSGIMRVSCSSAHGYGGKETLRTLKIVKENTNLEVLVNAGADFTENC
IKELKKHDIDIICCNLETINNKVFKSVKPGEDLENRINICKLVKKYDINLSSGLLLGIGESYEDRANHLLFLKELPVDEI
PIMGFNPYKDTPMENHPKCSALEQAKTIAISRLMFPDIRITSPTPTIGAELVQFALMGGASNIATVIPDNHPMNIKGVGN
PKTGNLKEVVKMIEELGLTPKIKK

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 38373; Mature: 38373

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSHIRDNFENLKEDTSKENLKNGLISKEDALKLFNISKGNDYLNLFSIASEVRDYFKK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
EIEIISSIHITNICSVNPKCYYCGFAAGTSKEGYYEPFRTTDEEIKNSAIAIEKSGIMRV
HHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEEEE
SCSSAHGYGGKETLRTLKIVKENTNLEVLVNAGADFTENCIKELKKHDIDIICCNLETIN
EECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECHHHHH
NKVFKSVKPGEDLENRINICKLVKKYDINLSSGLLLGIGESYEDRANHLLFLKELPVDEI
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCEEEEEECCCCCCC
PIMGFNPYKDTPMENHPKCSALEQAKTIAISRLMFPDIRITSPTPTIGAELVQFALMGGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCC
SNIATVIPDNHPMNIKGVGNPKTGNLKEVVKMIEELGLTPKIKK
CCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDFSHIRDNFENLKEDTSKENLKNGLISKEDALKLFNISKGNDYLNLFSIASEVRDYFKK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
EIEIISSIHITNICSVNPKCYYCGFAAGTSKEGYYEPFRTTDEEIKNSAIAIEKSGIMRV
HHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEEEE
SCSSAHGYGGKETLRTLKIVKENTNLEVLVNAGADFTENCIKELKKHDIDIICCNLETIN
EECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECHHHHH
NKVFKSVKPGEDLENRINICKLVKKYDINLSSGLLLGIGESYEDRANHLLFLKELPVDEI
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCEEEEEECCCCCCC
PIMGFNPYKDTPMENHPKCSALEQAKTIAISRLMFPDIRITSPTPTIGAELVQFALMGGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCC
SNIATVIPDNHPMNIKGVGNPKTGNLKEVVKMIEELGLTPKIKK
CCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]