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The map label for this gene is pycA [H]

Identifier: 150401430

GI number: 150401430

Start: 1031751

End: 1033280

Strand: Direct

Name: pycA [H]

Synonym: Maeo_1006

Alternate gene names: 150401430

Gene position: 1031751-1033280 (Clockwise)

Preceding gene: 150401429

Following gene: 150401431

Centisome position: 65.74

GC content: 34.38

Gene sequence:

>1530_bases
ATGTTTAAAAAAATATTAATCGCCAACAGAGGAGAAATTGCTGTTAGAATTATTAGAGCATGTCAGGAATTAGACATAAA
AACCGTAGCAATTTATTCTGAAGCCGATGAAAATGCATTATATACTTCAATAGCCGATGAATGTTATTGTATCGGCCCTC
CACAAGCTTCAAAAAGTTATCTTAATCTTGATATGATAGTCCATGTAGCAAAGAAAACAGGTGCAGAAGCAATTCACCCA
GGATATGGTTTTTTATCTGAAAATTTGGAATTTGCCAAAGCATGTGAGAATAATGGAATAGTATTTATTGGACCTCCGTC
GGAAGCCATAGATGCCATGGGTAGCAAAATAAATGCTAAAAAAATAATGAAAAATGCCAATGTTCCAGTTCTCCCTGGAA
GGGAAGAACCAATTGAAGACCCCGAAGAGGTTGTTGAAATTGCAGAAGAAATTGGCTACCCTGTAATTATTAAAGCCTCC
GCAGGTGGTGGAGGTATGGGAATGGCCGTGGCATACAATAAACAAGAATTAAAAGAAGTGGTAGAGTCCACAAAGTCAAT
AGCTCAAAGTGCATTTGGGGATTCCACTGTATTTATTGAAAAATATTTGGAAAACCCAAGACATATTGAAATCCAAGTAT
TGGGAGATAAATACGGAAATGTAGTTCATTTGGGAGATAGGGAGTGTTCCATTCAGAGAAGACATCAAAAACTTATTGAA
GAAGCTCCATCTCCAATAATGACAGAAGAATTAAGAAAAAAAATGGGGGATGCTGCAATTTCAGCAGCAAAAGCTATAAA
CTATTATAGTGCCGGAACTGTGGAATTCTTATACGATAATGGAGAATTTTATTTTTTGGAAATGAATACAAGAGTTCAAG
TTGAACATCCAATTACAGAAATTATCACAGGAGTAGATATTGTAAAGGAACAAATAAAAATAGCATATGGGGAAAAATTA
CCGTTCAAACAAGAGGACATTGTAATTAGAGGACATGCTATGGAATGCAGAATAAATGCAGAAGACCCCGTAAATGATTT
TGTTCCTACTCCCGGGGTAATTAAACATTATAGGTCCCCAGGAGGCCCGGGAATTAGAATAGATAGTGGCGTCTATGCCG
GTGCAGAAATTCCTCCATATTATGATTCATTAATATCTAAATTCATATCCTATGGAAATGATAGAGATGAAGCAATAGCA
AGAATGAAAAGAGCATTATCCGAATATATTATTATCGGACTTACCACAAATATACCATTCCATAGGGCAGTTATGGACGA
ATTAGACTTTAAAAATGGAAATATATCCACCCATTATATTGAAGAACATTCAGAATATTTAAAAGAAAATATAATGAAAT
ATGCCATTGAAGCTAAGGACGAAGAAAAATTATATGCCGATAAAATATTTGGAAAAAACAAACAAGTTGTAGCAATTGCA
GGTGGATTAAATGCATATATTTCAAGTGTTGTAGCTTCAAATAAAAATATAGATAAATGCGATGAAAAATATTCAGAAGA
GCAAAATTAA

Upstream 100 bases:

>100_bases
AATGTAGGAGACATACTTATGATTATAATATAAAATTATAATATAAAAAAACTAAAAATTAAATTAAAATACCCATATAT
AATATTAATAAGGGGATAGT

Downstream 100 bases:

>100_bases
CAAAACTTTTATTTGTTTTATTTTTCGGAGCTCCCATTATTAAATGCATATTTTTATTAAACATATAATATATAACTTAT
AAAATATATTTTTTTTAGAT

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MFKKILIANRGEIAVRIIRACQELDIKTVAIYSEADENALYTSIADECYCIGPPQASKSYLNLDMIVHVAKKTGAEAIHP
GYGFLSENLEFAKACENNGIVFIGPPSEAIDAMGSKINAKKIMKNANVPVLPGREEPIEDPEEVVEIAEEIGYPVIIKAS
AGGGGMGMAVAYNKQELKEVVESTKSIAQSAFGDSTVFIEKYLENPRHIEIQVLGDKYGNVVHLGDRECSIQRRHQKLIE
EAPSPIMTEELRKKMGDAAISAAKAINYYSAGTVEFLYDNGEFYFLEMNTRVQVEHPITEIITGVDIVKEQIKIAYGEKL
PFKQEDIVIRGHAMECRINAEDPVNDFVPTPGVIKHYRSPGGPGIRIDSGVYAGAEIPPYYDSLISKFISYGNDRDEAIA
RMKRALSEYIIIGLTTNIPFHRAVMDELDFKNGNISTHYIEEHSEYLKENIMKYAIEAKDEEKLYADKIFGKNKQVVAIA
GGLNAYISSVVASNKNIDKCDEKYSEEQN

Sequences:

>Translated_509_residues
MFKKILIANRGEIAVRIIRACQELDIKTVAIYSEADENALYTSIADECYCIGPPQASKSYLNLDMIVHVAKKTGAEAIHP
GYGFLSENLEFAKACENNGIVFIGPPSEAIDAMGSKINAKKIMKNANVPVLPGREEPIEDPEEVVEIAEEIGYPVIIKAS
AGGGGMGMAVAYNKQELKEVVESTKSIAQSAFGDSTVFIEKYLENPRHIEIQVLGDKYGNVVHLGDRECSIQRRHQKLIE
EAPSPIMTEELRKKMGDAAISAAKAINYYSAGTVEFLYDNGEFYFLEMNTRVQVEHPITEIITGVDIVKEQIKIAYGEKL
PFKQEDIVIRGHAMECRINAEDPVNDFVPTPGVIKHYRSPGGPGIRIDSGVYAGAEIPPYYDSLISKFISYGNDRDEAIA
RMKRALSEYIIIGLTTNIPFHRAVMDELDFKNGNISTHYIEEHSEYLKENIMKYAIEAKDEEKLYADKIFGKNKQVVAIA
GGLNAYISSVVASNKNIDKCDEKYSEEQN
>Mature_509_residues
MFKKILIANRGEIAVRIIRACQELDIKTVAIYSEADENALYTSIADECYCIGPPQASKSYLNLDMIVHVAKKTGAEAIHP
GYGFLSENLEFAKACENNGIVFIGPPSEAIDAMGSKINAKKIMKNANVPVLPGREEPIEDPEEVVEIAEEIGYPVIIKAS
AGGGGMGMAVAYNKQELKEVVESTKSIAQSAFGDSTVFIEKYLENPRHIEIQVLGDKYGNVVHLGDRECSIQRRHQKLIE
EAPSPIMTEELRKKMGDAAISAAKAINYYSAGTVEFLYDNGEFYFLEMNTRVQVEHPITEIITGVDIVKEQIKIAYGEKL
PFKQEDIVIRGHAMECRINAEDPVNDFVPTPGVIKHYRSPGGPGIRIDSGVYAGAEIPPYYDSLISKFISYGNDRDEAIA
RMKRALSEYIIIGLTTNIPFHRAVMDELDFKNGNISTHYIEEHSEYLKENIMKYAIEAKDEEKLYADKIFGKNKQVVAIA
GGLNAYISSVVASNKNIDKCDEKYSEEQN

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=444, Percent_Identity=53.3783783783784, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI65506442, Length=444, Percent_Identity=53.3783783783784, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI295821183, Length=444, Percent_Identity=53.3783783783784, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI116805327, Length=474, Percent_Identity=47.8902953586498, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI106049528, Length=452, Percent_Identity=46.6814159292035, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI106049295, Length=452, Percent_Identity=46.6814159292035, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI106049292, Length=452, Percent_Identity=46.6814159292035, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI134142062, Length=504, Percent_Identity=33.3333333333333, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI38679977, Length=504, Percent_Identity=31.547619047619, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI38679967, Length=504, Percent_Identity=31.547619047619, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI38679974, Length=504, Percent_Identity=31.547619047619, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI38679971, Length=504, Percent_Identity=31.547619047619, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI38679960, Length=504, Percent_Identity=31.547619047619, Blast_Score=255, Evalue=9e-68,
Organism=Homo sapiens, GI18105007, Length=336, Percent_Identity=22.9166666666667, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=55.079006772009, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1786216, Length=293, Percent_Identity=24.5733788395904, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17567343, Length=444, Percent_Identity=51.3513513513513, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71987519, Length=447, Percent_Identity=48.7695749440716, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17562816, Length=455, Percent_Identity=46.8131868131868, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI133931226, Length=490, Percent_Identity=31.0204081632653, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71997163, Length=507, Percent_Identity=29.9802761341223, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI71997168, Length=507, Percent_Identity=29.9802761341223, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI193204318, Length=275, Percent_Identity=23.6363636363636, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319695, Length=453, Percent_Identity=45.4746136865342, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=41.5730337078652, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321376, Length=453, Percent_Identity=46.1368653421634, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324343, Length=511, Percent_Identity=30.9197651663405, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6323863, Length=459, Percent_Identity=33.1154684095861, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322569, Length=205, Percent_Identity=24.390243902439, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24651757, Length=447, Percent_Identity=46.7561521252796, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI281363050, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652224, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652222, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652220, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652218, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652212, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652210, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652214, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI19921944, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652216, Length=454, Percent_Identity=48.0176211453744, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651759, Length=411, Percent_Identity=45.985401459854, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI161076409, Length=498, Percent_Identity=30.1204819277108, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI161076407, Length=495, Percent_Identity=30.3030303030303, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24586460, Length=495, Percent_Identity=30.3030303030303, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24586458, Length=495, Percent_Identity=30.3030303030303, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 56544; Mature: 56544

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKILIANRGEIAVRIIRACQELDIKTVAIYSEADENALYTSIADECYCIGPPQASKSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCEEECCCCCCCCHH
LNLDMIVHVAKKTGAEAIHPGYGFLSENLEFAKACENNGIVFIGPPSEAIDAMGSKINAK
EEHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHCCCCEEEECCCHHHHHHHCCCCCHH
KIMKNANVPVLPGREEPIEDPEEVVEIAEEIGYPVIIKASAGGGGMGMAVAYNKQELKEV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECHHHHHHH
VESTKSIAQSAFGDSTVFIEKYLENPRHIEIQVLGDKYGNVVHLGDRECSIQRRHQKLIE
HHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHH
EAPSPIMTEELRKKMGDAAISAAKAINYYSAGTVEFLYDNGEFYFLEMNTRVQVEHPITE
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCEEEECCCHHH
IITGVDIVKEQIKIAYGEKLPFKQEDIVIRGHAMECRINAEDPVNDFVPTPGVIKHYRSP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCCCHHCCCCCHHHHHCCCC
GGPGIRIDSGVYAGAEIPPYYDSLISKFISYGNDRDEAIARMKRALSEYIIIGLTTNIPF
CCCCEEECCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEEECCCCH
HRAVMDELDFKNGNISTHYIEEHSEYLKENIMKYAIEAKDEEKLYADKIFGKNKQVVAIA
HHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEE
GGLNAYISSVVASNKNIDKCDEKYSEEQN
CCHHHHHHHHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFKKILIANRGEIAVRIIRACQELDIKTVAIYSEADENALYTSIADECYCIGPPQASKSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCEEECCCCCCCCHH
LNLDMIVHVAKKTGAEAIHPGYGFLSENLEFAKACENNGIVFIGPPSEAIDAMGSKINAK
EEHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHCCCCEEEECCCHHHHHHHCCCCCHH
KIMKNANVPVLPGREEPIEDPEEVVEIAEEIGYPVIIKASAGGGGMGMAVAYNKQELKEV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECHHHHHHH
VESTKSIAQSAFGDSTVFIEKYLENPRHIEIQVLGDKYGNVVHLGDRECSIQRRHQKLIE
HHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHH
EAPSPIMTEELRKKMGDAAISAAKAINYYSAGTVEFLYDNGEFYFLEMNTRVQVEHPITE
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCEEEECCCHHH
IITGVDIVKEQIKIAYGEKLPFKQEDIVIRGHAMECRINAEDPVNDFVPTPGVIKHYRSP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCCCHHCCCCCHHHHHCCCC
GGPGIRIDSGVYAGAEIPPYYDSLISKFISYGNDRDEAIARMKRALSEYIIIGLTTNIPF
CCCCEEECCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEEECCCCH
HRAVMDELDFKNGNISTHYIEEHSEYLKENIMKYAIEAKDEEKLYADKIFGKNKQVVAIA
HHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEE
GGLNAYISSVVASNKNIDKCDEKYSEEQN
CCHHHHHHHHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]