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The map label for this gene is pycB [H]

Identifier: 150401429

GI number: 150401429

Start: 1029980

End: 1031683

Strand: Direct

Name: pycB [H]

Synonym: Maeo_1005

Alternate gene names: 150401429

Gene position: 1029980-1031683 (Clockwise)

Preceding gene: 150401428

Following gene: 150401430

Centisome position: 65.62

GC content: 34.27

Gene sequence:

>1704_bases
ATGGTAAAAATTACGGATACCACTCTTAGAGATGCACACCAATCTTTAATCGCTACAAGATTAAGAACCGAAGACATGAT
ACCCATCGCTGAGAAAATGGATGAAGTAGGATTTTATTCCATGGAAGTTTGGGGGGGTGCTACTTTTGATTCCTGTATTA
GATATTTAAATGAAGACCCATGGGAAAGATTAAGGGCACTTAGAAAAAGAGTTCAAAAAACACCATTGCAAATGCTTCTT
AGAGGGCAAAATTTAGTTGGATACAAACATTATTCAGACGACATAGTAGAAAAATTCATTGAAAAGTCATATGAAAATGG
AATAGAGATATTTAGAATATTTGATGCATTAAATGACATAAGAAATTTAGAAGTATCAATTAAGGCTGCAAAAAAATGCG
GAGCTCATGTTCAGGGAGCTATATCCTACACAATAAGTCCAGTTCATACCATAGATCAATATATATCACTTGCTAAGAAA
TTTGAAGAACTTGAATGTGATTCATTATGTATAAAAGATATGGCTGGGCTTTTAAAACCTTATGATGCCAAAATATTAAT
AAAAAGGTTGAAAAAAGAAATATCTATACCAATCAATTTGCATAGCCATTGTACAAGTGGATTAGCTCCAATGACATACA
ATGCCGCAATTGAAGCCGGTGTTGATATAGTGGATTGTGCCATTTCTCCATTATCAATGGGAACATCTCAACCACCAACT
GAAACATTTGTTTCGGCATTTAAAGGAACAAAATTTGATACGGGATTAGATACTGCTCTTTTAAATAAAATTAGGGAATA
CTTCGACGAAATTAGAAATAAATACAAATATTTAATAAACCCAATTTCAGAAAGAATCGATTCAAGAGTTTTGGTTTATC
AAGTTCCAGGGGGAATGTTGTCAAATCTTGTATCTCAATTAAAGGAACAGGGAGCCCTGGATAAATTCGAAGAAGTTCTT
AATGAAATACCACTAGTTAGAAAAGATTTAGGATATCCGCCACTTGTAACCCCATCATCCCAAATCGTAGGGACGCAGGC
AGTAATGAATGTTATTACTGGTGAAAGATACAAAGTAATTACAAATGAAGTTTCAAACTATGTAAAAGGACTTTATGGGA
AACCGCCTGCAAAAATAGATAGAGACCTTAAAAAAAGAGTTTTAGATAGCGAAGAAAAAGCAATTACCTGCAGACCTGCT
GATTTATTAAAACCAGAATATGAAAAAATCAAAGCCGATGCAGAGAGCAAAGGAATTGTGTCAAAAGAAGAGGACATATT
AACTTATGCATTATATCCACAAGTTGCCGTTAAGTTTTTAAGAGGGGAGCTCCAAGCCGAACCAATACCTGATGAAAAGG
AAGTGGCCAAATTCATGGAAATTCCAACAGAATATATTGTAGAAGTAGATGGAGACGAATTCGAAGTTAAAATCAAACCA
AGATATGGAACAGAAATGAAGAAAAAAGAAGATAAAATCACCGCCGACACAGAAGGTGCTTTAACTTCGCCATTTAGAGG
AATGATTACCCAAATAAAAGTTAAAGAAGGAGATGAAGTTAAAGAAGGAGATACCTTAATGATATTGGAAGCCATGAAAA
TGGAAAATCCTGTGGGAGCTCCTGCTGATGGAAAAGTTAAAAAAATAGTTGTTCATGAAGGTCAGTCTGTCAATGTAGGA
GACATACTTATGATTATAATATAA

Upstream 100 bases:

>100_bases
TGTTTGGGAGCTCCACTATACACTACTTTTTAGGAGCCCCACTTCAAAGCATTTTTTTATTAAATATAATTTTTATTTTA
TAACTAAAAGAGGGATAATT

Downstream 100 bases:

>100_bases
AATTATAATATAAAAAAACTAAAAATTAAATTAAAATACCCATATATAATATTAATAAGGGGATAGTATGTTTAAAAAAA
TATTAATCGCCAACAGAGGA

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MVKITDTTLRDAHQSLIATRLRTEDMIPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDPWERLRALRKRVQKTPLQMLL
RGQNLVGYKHYSDDIVEKFIEKSYENGIEIFRIFDALNDIRNLEVSIKAAKKCGAHVQGAISYTISPVHTIDQYISLAKK
FEELECDSLCIKDMAGLLKPYDAKILIKRLKKEISIPINLHSHCTSGLAPMTYNAAIEAGVDIVDCAISPLSMGTSQPPT
ETFVSAFKGTKFDTGLDTALLNKIREYFDEIRNKYKYLINPISERIDSRVLVYQVPGGMLSNLVSQLKEQGALDKFEEVL
NEIPLVRKDLGYPPLVTPSSQIVGTQAVMNVITGERYKVITNEVSNYVKGLYGKPPAKIDRDLKKRVLDSEEKAITCRPA
DLLKPEYEKIKADAESKGIVSKEEDILTYALYPQVAVKFLRGELQAEPIPDEKEVAKFMEIPTEYIVEVDGDEFEVKIKP
RYGTEMKKKEDKITADTEGALTSPFRGMITQIKVKEGDEVKEGDTLMILEAMKMENPVGAPADGKVKKIVVHEGQSVNVG
DILMIII

Sequences:

>Translated_567_residues
MVKITDTTLRDAHQSLIATRLRTEDMIPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDPWERLRALRKRVQKTPLQMLL
RGQNLVGYKHYSDDIVEKFIEKSYENGIEIFRIFDALNDIRNLEVSIKAAKKCGAHVQGAISYTISPVHTIDQYISLAKK
FEELECDSLCIKDMAGLLKPYDAKILIKRLKKEISIPINLHSHCTSGLAPMTYNAAIEAGVDIVDCAISPLSMGTSQPPT
ETFVSAFKGTKFDTGLDTALLNKIREYFDEIRNKYKYLINPISERIDSRVLVYQVPGGMLSNLVSQLKEQGALDKFEEVL
NEIPLVRKDLGYPPLVTPSSQIVGTQAVMNVITGERYKVITNEVSNYVKGLYGKPPAKIDRDLKKRVLDSEEKAITCRPA
DLLKPEYEKIKADAESKGIVSKEEDILTYALYPQVAVKFLRGELQAEPIPDEKEVAKFMEIPTEYIVEVDGDEFEVKIKP
RYGTEMKKKEDKITADTEGALTSPFRGMITQIKVKEGDEVKEGDTLMILEAMKMENPVGAPADGKVKKIVVHEGQSVNVG
DILMIII
>Mature_567_residues
MVKITDTTLRDAHQSLIATRLRTEDMIPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDPWERLRALRKRVQKTPLQMLL
RGQNLVGYKHYSDDIVEKFIEKSYENGIEIFRIFDALNDIRNLEVSIKAAKKCGAHVQGAISYTISPVHTIDQYISLAKK
FEELECDSLCIKDMAGLLKPYDAKILIKRLKKEISIPINLHSHCTSGLAPMTYNAAIEAGVDIVDCAISPLSMGTSQPPT
ETFVSAFKGTKFDTGLDTALLNKIREYFDEIRNKYKYLINPISERIDSRVLVYQVPGGMLSNLVSQLKEQGALDKFEEVL
NEIPLVRKDLGYPPLVTPSSQIVGTQAVMNVITGERYKVITNEVSNYVKGLYGKPPAKIDRDLKKRVLDSEEKAITCRPA
DLLKPEYEKIKADAESKGIVSKEEDILTYALYPQVAVKFLRGELQAEPIPDEKEVAKFMEIPTEYIVEVDGDEFEVKIKP
RYGTEMKKKEDKITADTEGALTSPFRGMITQIKVKEGDEVKEGDTLMILEAMKMENPVGAPADGKVKKIVVHEGQSVNVG
DILMIII

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=618, Percent_Identity=32.8478964401294, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI106049295, Length=618, Percent_Identity=32.8478964401294, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI106049292, Length=618, Percent_Identity=32.8478964401294, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI65506442, Length=95, Percent_Identity=37.8947368421053, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI189095269, Length=95, Percent_Identity=37.8947368421053, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562816, Length=617, Percent_Identity=34.6839546191248, Blast_Score=315, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6319695, Length=612, Percent_Identity=33.4967320261438, Blast_Score=335, Evalue=8e-93,
Organism=Saccharomyces cerevisiae, GI6321376, Length=612, Percent_Identity=33.6601307189542, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI281363050, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652224, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652222, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652212, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652220, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652210, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652214, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI19921944, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652218, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652216, Length=615, Percent_Identity=34.7967479674797, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 63761; Mature: 63761

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKITDTTLRDAHQSLIATRLRTEDMIPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDP
CEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
WERLRALRKRVQKTPLQMLLRGQNLVGYKHYSDDIVEKFIEKSYENGIEIFRIFDALNDI
HHHHHHHHHHHHHCHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RNLEVSIKAAKKCGAHVQGAISYTISPVHTIDQYISLAKKFEELECDSLCIKDMAGLLKP
HCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
YDAKILIKRLKKEISIPINLHSHCTSGLAPMTYNAAIEAGVDIVDCAISPLSMGTSQPPT
CCHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEHHHHHCCCHHHHHHCCCCCCCCCCCCH
ETFVSAFKGTKFDTGLDTALLNKIREYFDEIRNKYKYLINPISERIDSRVLVYQVPGGML
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
SNLVSQLKEQGALDKFEEVLNEIPLVRKDLGYPPLVTPSSQIVGTQAVMNVITGERYKVI
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
TNEVSNYVKGLYGKPPAKIDRDLKKRVLDSEEKAITCRPADLLKPEYEKIKADAESKGIV
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHCCCHHHHHHCCCHHCCCC
SKEEDILTYALYPQVAVKFLRGELQAEPIPDEKEVAKFMEIPTEYIVEVDGDEFEVKIKP
CCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHEEEECCCEEEEEECC
RYGTEMKKKEDKITADTEGALTSPFRGMITQIKVKEGDEVKEGDTLMILEAMKMENPVGA
CCCCCHHHHHCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCEEEEEEHHHHCCCCCC
PADGKVKKIVVHEGQSVNVGDILMIII
CCCCCEEEEEEECCCCCCCCCEEEEEC
>Mature Secondary Structure
MVKITDTTLRDAHQSLIATRLRTEDMIPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDP
CEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
WERLRALRKRVQKTPLQMLLRGQNLVGYKHYSDDIVEKFIEKSYENGIEIFRIFDALNDI
HHHHHHHHHHHHHCHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RNLEVSIKAAKKCGAHVQGAISYTISPVHTIDQYISLAKKFEELECDSLCIKDMAGLLKP
HCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
YDAKILIKRLKKEISIPINLHSHCTSGLAPMTYNAAIEAGVDIVDCAISPLSMGTSQPPT
CCHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEHHHHHCCCHHHHHHCCCCCCCCCCCCH
ETFVSAFKGTKFDTGLDTALLNKIREYFDEIRNKYKYLINPISERIDSRVLVYQVPGGML
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
SNLVSQLKEQGALDKFEEVLNEIPLVRKDLGYPPLVTPSSQIVGTQAVMNVITGERYKVI
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
TNEVSNYVKGLYGKPPAKIDRDLKKRVLDSEEKAITCRPADLLKPEYEKIKADAESKGIV
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHCCCHHHHHHCCCHHCCCC
SKEEDILTYALYPQVAVKFLRGELQAEPIPDEKEVAKFMEIPTEYIVEVDGDEFEVKIKP
CCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHEEEECCCEEEEEECC
RYGTEMKKKEDKITADTEGALTSPFRGMITQIKVKEGDEVKEGDTLMILEAMKMENPVGA
CCCCCHHHHHCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCEEEEEEHHHHCCCCCC
PADGKVKKIVVHEGQSVNVGDILMIII
CCCCCEEEEEEECCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]