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The map label for this gene is aksA [H]

Identifier: 150401418

GI number: 150401418

Start: 1018390

End: 1019553

Strand: Reverse

Name: aksA [H]

Synonym: Maeo_0994

Alternate gene names: 150401418

Gene position: 1019553-1018390 (Counterclockwise)

Preceding gene: 150401419

Following gene: 150401417

Centisome position: 64.96

GC content: 34.88

Gene sequence:

>1164_bases
ATGAATTGGAAAGAAGTTTGTCAATATAATCCAAAGTTGAATTTGGAGGATTGCTATATTTATGATACTACACTTAGAGA
TGGGGAGCAAACTCCGGGAGTTTGTTTTTCAATGGAACAAAAATTGGATATTGCTAAAAAATTAGATGAATTGGGGGTAA
AGCAGATTGAAGCAGGTTTTCCAGCCGTATCTAAATCAGAAATAGAAAATGTAAAAAAAATAGCAAACGAAGGACTAAAC
GCTGAAATATTGGCGTTATCTCGGGCACTACAAGGAGATATTGATAAGGCATTATCCTGTGATGTAGATGGGATTATTAC
ATTTATAGCAGCTTCTCCTCTTCATTTAAAATATAAACTTCATAAAAGCATCGAGGAAGTAGAAGAGATGGGAATGAAGG
CAGTGGAATATGCAAAAGACCACGGGCTATTTGTAGCTTTTTCGGCTGAAGATGCTACAAGGACACCAATTGAGGACCTT
GTAAGAATTCATAAAAATGCAGAGGAACACGGAGCAGATAGGGTTCATATAGCAGATACTACGGGCTGTGGAACACCACA
ATCCATACAATATATATGTTCAGAACTTAGCAATAATTTAAAAAAGGCACATATTGGAGTTCATTGCCATAATGATTTTG
GACTTGCCGTAATAAATTCTATTTATGGACTATTGGGTGGTGCAAAAGCAGCATCTACCACAGTAAATGGAATTGGGGAA
AGAGCTGGAAATGCACCTTTGGAGGAGCTCCTATTAACTATGAATGTATTATATGATGTAAAAACTGATTTAAATATCTC
AATAATAAAGGAATTATCGACAATGGTTGAAAACTATTCGGGGATAAAAATACCAGTAAATAAGCCGATAGTAGGGGATA
AAGTATTTTATCATGAAAGCGGAATTCATGTTGATGCAGTAATTGAAAATCCACTAACTTATGAACCATTTTTGCCGGAA
AGAATCGGGCAAAAACGAGAAATTGTATTGGGAAAACATTCTGGTTGTAGTGCAGTCGAATCAAAATTAAAAGAGCTTGG
CTTAGAAGTTCCAAAAGATAGAATATGGGATTTAGTTAAAAAAGTAAAAACCACCAGAGAAGGCGGGGAAGATATAGACG
ATGAGATGTTTATAAAAATAGTAGATATAATAAATAAACAATAA

Upstream 100 bases:

>100_bases
AATAATTACTATTGCCATATATATTTATATTATTATATATTACTATTATAAATTATTATAAATTATATTTTATTACTATA
CTATTTACTGGTGAAATTAC

Downstream 100 bases:

>100_bases
ATTAATCATTTTATAATTAAAAAATTCGTGTGATGAAATGAATGGAATAAAAAAAATATCGTTATTAATATTATCAATTA
TTTTAATTGGTAGTATGGCA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNWKEVCQYNPKLNLEDCYIYDTTLRDGEQTPGVCFSMEQKLDIAKKLDELGVKQIEAGFPAVSKSEIENVKKIANEGLN
AEILALSRALQGDIDKALSCDVDGIITFIAASPLHLKYKLHKSIEEVEEMGMKAVEYAKDHGLFVAFSAEDATRTPIEDL
VRIHKNAEEHGADRVHIADTTGCGTPQSIQYICSELSNNLKKAHIGVHCHNDFGLAVINSIYGLLGGAKAASTTVNGIGE
RAGNAPLEELLLTMNVLYDVKTDLNISIIKELSTMVENYSGIKIPVNKPIVGDKVFYHESGIHVDAVIENPLTYEPFLPE
RIGQKREIVLGKHSGCSAVESKLKELGLEVPKDRIWDLVKKVKTTREGGEDIDDEMFIKIVDIINKQ

Sequences:

>Translated_387_residues
MNWKEVCQYNPKLNLEDCYIYDTTLRDGEQTPGVCFSMEQKLDIAKKLDELGVKQIEAGFPAVSKSEIENVKKIANEGLN
AEILALSRALQGDIDKALSCDVDGIITFIAASPLHLKYKLHKSIEEVEEMGMKAVEYAKDHGLFVAFSAEDATRTPIEDL
VRIHKNAEEHGADRVHIADTTGCGTPQSIQYICSELSNNLKKAHIGVHCHNDFGLAVINSIYGLLGGAKAASTTVNGIGE
RAGNAPLEELLLTMNVLYDVKTDLNISIIKELSTMVENYSGIKIPVNKPIVGDKVFYHESGIHVDAVIENPLTYEPFLPE
RIGQKREIVLGKHSGCSAVESKLKELGLEVPKDRIWDLVKKVKTTREGGEDIDDEMFIKIVDIINKQ
>Mature_387_residues
MNWKEVCQYNPKLNLEDCYIYDTTLRDGEQTPGVCFSMEQKLDIAKKLDELGVKQIEAGFPAVSKSEIENVKKIANEGLN
AEILALSRALQGDIDKALSCDVDGIITFIAASPLHLKYKLHKSIEEVEEMGMKAVEYAKDHGLFVAFSAEDATRTPIEDL
VRIHKNAEEHGADRVHIADTTGCGTPQSIQYICSELSNNLKKAHIGVHCHNDFGLAVINSIYGLLGGAKAASTTVNGIGE
RAGNAPLEELLLTMNVLYDVKTDLNISIIKELSTMVENYSGIKIPVNKPIVGDKVFYHESGIHVDAVIENPLTYEPFLPE
RIGQKREIVLGKHSGCSAVESKLKELGLEVPKDRIWDLVKKVKTTREGGEDIDDEMFIKIVDIINKQ

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=357, Percent_Identity=40.0560224089636, Blast_Score=230, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6320071, Length=353, Percent_Identity=33.4277620396601, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6320019, Length=353, Percent_Identity=33.4277620396601, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6324682, Length=402, Percent_Identity=26.1194029850746, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324225, Length=374, Percent_Identity=26.7379679144385, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24582381, Length=253, Percent_Identity=22.9249011857708, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42772; Mature: 42772

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKEVCQYNPKLNLEDCYIYDTTLRDGEQTPGVCFSMEQKLDIAKKLDELGVKQIEAGF
CCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHCCC
PAVSKSEIENVKKIANEGLNAEILALSRALQGDIDKALSCDVDGIITFIAASPLHLKYKL
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCEEHHHH
HKSIEEVEEMGMKAVEYAKDHGLFVAFSAEDATRTPIEDLVRIHKNAEEHGADRVHIADT
HHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCHHHCCCCEEEEECC
TGCGTPQSIQYICSELSNNLKKAHIGVHCHNDFGLAVINSIYGLLGGAKAASTTVNGIGE
CCCCCHHHHHHHHHHHHCCHHHHEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RAGNAPLEELLLTMNVLYDVKTDLNISIIKELSTMVENYSGIKIPVNKPIVGDKVFYHES
HCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECC
GIHVDAVIENPLTYEPFLPERIGQKREIVLGKHSGCSAVESKLKELGLEVPKDRIWDLVK
CCEEEEEECCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHH
KVKTTREGGEDIDDEMFIKIVDIINKQ
HHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNWKEVCQYNPKLNLEDCYIYDTTLRDGEQTPGVCFSMEQKLDIAKKLDELGVKQIEAGF
CCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHCCC
PAVSKSEIENVKKIANEGLNAEILALSRALQGDIDKALSCDVDGIITFIAASPLHLKYKL
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCEEHHHH
HKSIEEVEEMGMKAVEYAKDHGLFVAFSAEDATRTPIEDLVRIHKNAEEHGADRVHIADT
HHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCHHHCCCCEEEEECC
TGCGTPQSIQYICSELSNNLKKAHIGVHCHNDFGLAVINSIYGLLGGAKAASTTVNGIGE
CCCCCHHHHHHHHHHHHCCHHHHEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RAGNAPLEELLLTMNVLYDVKTDLNISIIKELSTMVENYSGIKIPVNKPIVGDKVFYHES
HCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECC
GIHVDAVIENPLTYEPFLPERIGQKREIVLGKHSGCSAVESKLKELGLEVPKDRIWDLVK
CCEEEEEECCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHH
KVKTTREGGEDIDDEMFIKIVDIINKQ
HHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]