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The map label for this gene is hyfF [H]

Identifier: 150401365

GI number: 150401365

Start: 962838

End: 964250

Strand: Reverse

Name: hyfF [H]

Synonym: Maeo_0940

Alternate gene names: 150401365

Gene position: 964250-962838 (Counterclockwise)

Preceding gene: 150401366

Following gene: 150401364

Centisome position: 61.44

GC content: 32.34

Gene sequence:

>1413_bases
ATGGATGAATTAGTAGTTATAATTCCTGCATTAGCTGGGGTTATTGCCTACTTTATAAAGGGAAAGCTTACAAGATATAT
TCCATTTATAGCAGCAATAATTCAGGTTATTATTACAGCATATATATTTATTGAACCTAGCCAAGTAATATTGTTAAAAA
ATTATATATTTATCACACCACTTGGAAAAATAGTGCTTGGTATAATTTCGTTATTATTTTTAATGGTGTCATATTATTCC
ATAGACTACTTTAAAAATTCCAACTATGAATCAGAACATATTTACAGTTCTATGCTGTTGTTCTTTGTGTCTGCAATGAG
TTTGACGGCAATTTCTGACCATATAATCCTTACATGGATAGCTATTGAAGCTACAACAATTTCAAGTGCTCCGCTGATAT
TTTTGCATAAAACAAGGGATTCAGTCATGGCTACATGGAAATATATTATTACCTGTTCCGTAGGAATTTCACTTGCATTA
TTGGGCTCTTTCATTATATTACAATCGTTTACACAGCCTATTCAAGTTTCTGGGCTTTTATTCTCTAATCTAATGGCATA
TACTGGAACAATAAATCCATTGTGGTTTTTAGTTGGATTTGTGTTTATACTCATAGGATATGGAACAAAAGTTGGTTTAG
CTCCCATGCATATCTGGCTTTCAGATGCTCATGGGGAAGCTCCAAGTCCTGCATCGGCACTACTTTCGGGAGCTCTGTTA
AATTGTGCTTTCTTACCCATATATAAAATCAATGTTCTTGCAGGATATCTTAATTTGGGAGAAATTACAGGCAAAATGAT
GATAGCTCTTGGTTTATTCTCTGTATTTGTGGCTGCTGCATTTATGCCTGCTCAAAAGGATTATAAAAGATTGCTTGCAT
ATTCTAGTATTGAAAACATGGGTATTATAGTATTGGGGGCTGGAATTGGAGGTATAGCATTGCTTGGGTCGATTTTCCAC
ATGATAAATCATTCACTTATTAAATGTGCTCTGTTTTTAGCTACTGGAAATATGCTTCTTCAATATGGCACAAAAGATAT
ATCCAAAGTAAAAAATTTCTTTAAATTGTTGCCAAAAACAGCATTTGCATTTACGCTTGGGGCAATTGGTATTTCAGGAT
TTCCGCCGTTTGGATTGTTTCTAAGTGAATTATTAATAATATTTGGAGCATTTACTGGACATTACTATGCAGTGGGATTT
TTATTTATTATTGCATTGATTTTGGTAATTGTAGGATTCTCTAAAAAAATTATTCAAATATGTTATAGTGAATCAGAGGA
ATGTAACATTAAACCGATGAAAGAAAGCTTTGGGCTGTATGCTCCAAGTTTAATTATGTTATTCATTTCGCTTATAATTA
CTGCAATTATGTTTAGTTCATATCAAGACTATATATTAAATTTTATACAATAG

Upstream 100 bases:

>100_bases
TAACAAACTTTACACTACCGAAGACTACAAAAAAGAATAACGGACTGCAAAAATCATTATAATAATTTAATTAAAATGTA
TGGATATAGAGGCGAATAAG

Downstream 100 bases:

>100_bases
TTCAAATGTTTGAATATAATAGTTAATCTTCAATGGCAGTCCTAATTATTGAATAAAATCCTTTTAGGATTTTATACAAA
AGCATAGCTTTTGTAAACAA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MDELVVIIPALAGVIAYFIKGKLTRYIPFIAAIIQVIITAYIFIEPSQVILLKNYIFITPLGKIVLGIISLLFLMVSYYS
IDYFKNSNYESEHIYSSMLLFFVSAMSLTAISDHIILTWIAIEATTISSAPLIFLHKTRDSVMATWKYIITCSVGISLAL
LGSFIILQSFTQPIQVSGLLFSNLMAYTGTINPLWFLVGFVFILIGYGTKVGLAPMHIWLSDAHGEAPSPASALLSGALL
NCAFLPIYKINVLAGYLNLGEITGKMMIALGLFSVFVAAAFMPAQKDYKRLLAYSSIENMGIIVLGAGIGGIALLGSIFH
MINHSLIKCALFLATGNMLLQYGTKDISKVKNFFKLLPKTAFAFTLGAIGISGFPPFGLFLSELLIIFGAFTGHYYAVGF
LFIIALILVIVGFSKKIIQICYSESEECNIKPMKESFGLYAPSLIMLFISLIITAIMFSSYQDYILNFIQ

Sequences:

>Translated_470_residues
MDELVVIIPALAGVIAYFIKGKLTRYIPFIAAIIQVIITAYIFIEPSQVILLKNYIFITPLGKIVLGIISLLFLMVSYYS
IDYFKNSNYESEHIYSSMLLFFVSAMSLTAISDHIILTWIAIEATTISSAPLIFLHKTRDSVMATWKYIITCSVGISLAL
LGSFIILQSFTQPIQVSGLLFSNLMAYTGTINPLWFLVGFVFILIGYGTKVGLAPMHIWLSDAHGEAPSPASALLSGALL
NCAFLPIYKINVLAGYLNLGEITGKMMIALGLFSVFVAAAFMPAQKDYKRLLAYSSIENMGIIVLGAGIGGIALLGSIFH
MINHSLIKCALFLATGNMLLQYGTKDISKVKNFFKLLPKTAFAFTLGAIGISGFPPFGLFLSELLIIFGAFTGHYYAVGF
LFIIALILVIVGFSKKIIQICYSESEECNIKPMKESFGLYAPSLIMLFISLIITAIMFSSYQDYILNFIQ
>Mature_470_residues
MDELVVIIPALAGVIAYFIKGKLTRYIPFIAAIIQVIITAYIFIEPSQVILLKNYIFITPLGKIVLGIISLLFLMVSYYS
IDYFKNSNYESEHIYSSMLLFFVSAMSLTAISDHIILTWIAIEATTISSAPLIFLHKTRDSVMATWKYIITCSVGISLAL
LGSFIILQSFTQPIQVSGLLFSNLMAYTGTINPLWFLVGFVFILIGYGTKVGLAPMHIWLSDAHGEAPSPASALLSGALL
NCAFLPIYKINVLAGYLNLGEITGKMMIALGLFSVFVAAAFMPAQKDYKRLLAYSSIENMGIIVLGAGIGGIALLGSIFH
MINHSLIKCALFLATGNMLLQYGTKDISKVKNFFKLLPKTAFAFTLGAIGISGFPPFGLFLSELLIIFGAFTGHYYAVGF
LFIIALILVIVGFSKKIIQICYSESEECNIKPMKESFGLYAPSLIMLFISLIITAIMFSSYQDYILNFIQ

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=342, Percent_Identity=40.0584795321637, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1788827, Length=321, Percent_Identity=30.2180685358255, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1788829, Length=337, Percent_Identity=26.4094955489614, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788613, Length=191, Percent_Identity=30.8900523560209, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367154, Length=345, Percent_Identity=26.9565217391304, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788614, Length=408, Percent_Identity=24.0196078431373, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51656; Mature: 51656

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELVVIIPALAGVIAYFIKGKLTRYIPFIAAIIQVIITAYIFIEPSQVILLKNYIFITP
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECEEEECC
LGKIVLGIISLLFLMVSYYSIDYFKNSNYESEHIYSSMLLFFVSAMSLTAISDHIILTWI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AIEATTISSAPLIFLHKTRDSVMATWKYIITCSVGISLALLGSFIILQSFTQPIQVSGLL
EEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FSNLMAYTGTINPLWFLVGFVFILIGYGTKVGLAPMHIWLSDAHGEAPSPASALLSGALL
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
NCAFLPIYKINVLAGYLNLGEITGKMMIALGLFSVFVAAAFMPAQKDYKRLLAYSSIENM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
GIIVLGAGIGGIALLGSIFHMINHSLIKCALFLATGNMLLQYGTKDISKVKNFFKLLPKT
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHH
AFAFTLGAIGISGFPPFGLFLSELLIIFGAFTGHYYAVGFLFIIALILVIVGFSKKIIQI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
CYSESEECNIKPMKESFGLYAPSLIMLFISLIITAIMFSSYQDYILNFIQ
HHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDELVVIIPALAGVIAYFIKGKLTRYIPFIAAIIQVIITAYIFIEPSQVILLKNYIFITP
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECEEEECC
LGKIVLGIISLLFLMVSYYSIDYFKNSNYESEHIYSSMLLFFVSAMSLTAISDHIILTWI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AIEATTISSAPLIFLHKTRDSVMATWKYIITCSVGISLALLGSFIILQSFTQPIQVSGLL
EEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FSNLMAYTGTINPLWFLVGFVFILIGYGTKVGLAPMHIWLSDAHGEAPSPASALLSGALL
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
NCAFLPIYKINVLAGYLNLGEITGKMMIALGLFSVFVAAAFMPAQKDYKRLLAYSSIENM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
GIIVLGAGIGGIALLGSIFHMINHSLIKCALFLATGNMLLQYGTKDISKVKNFFKLLPKT
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHH
AFAFTLGAIGISGFPPFGLFLSELLIIFGAFTGHYYAVGFLFIIALILVIVGFSKKIIQI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
CYSESEECNIKPMKESFGLYAPSLIMLFISLIITAIMFSSYQDYILNFIQ
HHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]