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The map label for this gene is hyfG [H]

Identifier: 150401364

GI number: 150401364

Start: 960976

End: 962532

Strand: Reverse

Name: hyfG [H]

Synonym: Maeo_0939

Alternate gene names: 150401364

Gene position: 962532-960976 (Counterclockwise)

Preceding gene: 150401365

Following gene: 150401363

Centisome position: 61.33

GC content: 34.3

Gene sequence:

>1557_bases
ATGGGATTATTAAAAATAGATAATGGAATGGCAGTAAATGTTGAAGATATACCTATAATTCCATTTGAGGAATTCAAGGA
TAAAATTTTAAAATTTACAGAAACCGGATATGTTGTTCAGCATTTTGCAATTCCCTCTTATAATATTAATAAAAATAATA
GCAATGAAAATAAATATAAAATATATTCAGTTTTGAGAAATGATACAGAATTATATGTATTAAGCACCATGGTGGGAACC
TCCTTTGAATCACTTTCACAATATAATATTAAATTTCAAATGTTTGAAAGGGAAATAGCAGAACAATATGGAATTGTTCC
TAAAAATCACCCATGGCTTAAATCTGTAAGATACCATAAAAATTATAAGGGAGCTCCCGATGTATTTGGTAATAATTACA
CAGCACCTATTCCTGGAAATTATCCATTCTATGAGGTGGGCGGTGAAGAAATACATCAAGTTGCAGTTGGTCCAGTTCAT
GCGGGAATTATTGAGCCCGGACATTTTAGATTTAACTGTATCGGGGAAACTATTTTAAGTCTTGAAATCCAACATGGATA
TCAACATAGAGGCATCGAAAAACAAATATTAAATTGTAAAAAAGAAGTATTGCCATTAATTATCGAATCCATAGCAGGAG
ATACTGTAATTGGCAATAGTATCTGTTTTTCGGAAGCTATTGAATGGTTAAGTAATTGTAATTATGAAGCAAATGAGCCA
CTATATAATTATAGAAGATTATTATTGGAAATAGAAAGGATAGCCAACCATATCGGAACGATGGGCGGTCTTGCAGGAGA
TGTGGGATATATACCTACGGCATCATATTACGGTAGGATCAGGGGGGACTTTTTAAATATGTTTGTATTGATATGCGGAA
ATAGATTTGGTAGGAGCTCAGTGCGACCTTTCGGAGCTCCTTTTAAACTCAGCGATGACCACATAAAAGAGCTCATTAAA
AGGTTTGAACAGCTTAAAAAAGAAGTTATTGATGTAGGCAATCTAATGCTAGATAATCCAGAAGTTCTTGGAAGATTTGA
CTACACGGGTATAGTAGATACAAAAACAGCCAAGTTAATAGGTCTTGTAGGTCCAGCAGGTAGGGCATCGGGAATAAAAT
ATGATGTAAGGAAGAGCTTTTCAAAGGCTAAGAATATTTACAACCGTGGTATAGGGCTCGTAGCTAGAAAAGACGGAGCT
GTTAGTTCCAGGGCAAAAACATACTTTTACGAAACCCTTGACTCCATAGAATTTTGCATAGAGCTCCTTAAAGTCGCAGA
TTTCCAAATCAATGACAGTGCCGATAAATTAGAAAAAATAGAAAAAACAGTATTAAAACCTAACAGCTTTATAATAACCA
TGGAAGAAGCTTGGAGAGGTGAGCTCTCCCATTGTATAATCACGGATGAGAATGGAAACATAATTAGATATAAAATAAAA
GACCCATCATTCCACAACTGGACAGCACTTGAATTGGTAGTTAGAAATGAAGGAATATATGATTTCCCTCTGTGCAACAA
AAGTTTTAACTTATCATACTGTGGTTTTGACCTTTAA

Upstream 100 bases:

>100_bases
TTGTTCGAAGATTGACTATAAATTAATATTAAAAAGGAGCTCCCTGTGAGGCATTCTTAAAATAAAATTATAATTATATA
ATTTATATAAGGTGAATTAA

Downstream 100 bases:

>100_bases
TGAGGTGTATATATGAACAACATAATTATCAACCGGATAACGCAGGGATATAGGACAGTTGAATTTCCCAGTGATAAAAT
ACAGCTTCCCGAAGCATACC

Product: hydrogenase, component E-formate hydrogenlyase subunit 5-like protein

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MGLLKIDNGMAVNVEDIPIIPFEEFKDKILKFTETGYVVQHFAIPSYNINKNNSNENKYKIYSVLRNDTELYVLSTMVGT
SFESLSQYNIKFQMFEREIAEQYGIVPKNHPWLKSVRYHKNYKGAPDVFGNNYTAPIPGNYPFYEVGGEEIHQVAVGPVH
AGIIEPGHFRFNCIGETILSLEIQHGYQHRGIEKQILNCKKEVLPLIIESIAGDTVIGNSICFSEAIEWLSNCNYEANEP
LYNYRRLLLEIERIANHIGTMGGLAGDVGYIPTASYYGRIRGDFLNMFVLICGNRFGRSSVRPFGAPFKLSDDHIKELIK
RFEQLKKEVIDVGNLMLDNPEVLGRFDYTGIVDTKTAKLIGLVGPAGRASGIKYDVRKSFSKAKNIYNRGIGLVARKDGA
VSSRAKTYFYETLDSIEFCIELLKVADFQINDSADKLEKIEKTVLKPNSFIITMEEAWRGELSHCIITDENGNIIRYKIK
DPSFHNWTALELVVRNEGIYDFPLCNKSFNLSYCGFDL

Sequences:

>Translated_518_residues
MGLLKIDNGMAVNVEDIPIIPFEEFKDKILKFTETGYVVQHFAIPSYNINKNNSNENKYKIYSVLRNDTELYVLSTMVGT
SFESLSQYNIKFQMFEREIAEQYGIVPKNHPWLKSVRYHKNYKGAPDVFGNNYTAPIPGNYPFYEVGGEEIHQVAVGPVH
AGIIEPGHFRFNCIGETILSLEIQHGYQHRGIEKQILNCKKEVLPLIIESIAGDTVIGNSICFSEAIEWLSNCNYEANEP
LYNYRRLLLEIERIANHIGTMGGLAGDVGYIPTASYYGRIRGDFLNMFVLICGNRFGRSSVRPFGAPFKLSDDHIKELIK
RFEQLKKEVIDVGNLMLDNPEVLGRFDYTGIVDTKTAKLIGLVGPAGRASGIKYDVRKSFSKAKNIYNRGIGLVARKDGA
VSSRAKTYFYETLDSIEFCIELLKVADFQINDSADKLEKIEKTVLKPNSFIITMEEAWRGELSHCIITDENGNIIRYKIK
DPSFHNWTALELVVRNEGIYDFPLCNKSFNLSYCGFDL
>Mature_517_residues
GLLKIDNGMAVNVEDIPIIPFEEFKDKILKFTETGYVVQHFAIPSYNINKNNSNENKYKIYSVLRNDTELYVLSTMVGTS
FESLSQYNIKFQMFEREIAEQYGIVPKNHPWLKSVRYHKNYKGAPDVFGNNYTAPIPGNYPFYEVGGEEIHQVAVGPVHA
GIIEPGHFRFNCIGETILSLEIQHGYQHRGIEKQILNCKKEVLPLIIESIAGDTVIGNSICFSEAIEWLSNCNYEANEPL
YNYRRLLLEIERIANHIGTMGGLAGDVGYIPTASYYGRIRGDFLNMFVLICGNRFGRSSVRPFGAPFKLSDDHIKELIKR
FEQLKKEVIDVGNLMLDNPEVLGRFDYTGIVDTKTAKLIGLVGPAGRASGIKYDVRKSFSKAKNIYNRGIGLVARKDGAV
SSRAKTYFYETLDSIEFCIELLKVADFQINDSADKLEKIEKTVLKPNSFIITMEEAWRGELSHCIITDENGNIIRYKIKD
PSFHNWTALELVVRNEGIYDFPLCNKSFNLSYCGFDL

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788832, Length=436, Percent_Identity=24.3119266055046, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789076, Length=438, Percent_Identity=23.7442922374429, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24638644, Length=423, Percent_Identity=25.7683215130024, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 58806; Mature: 58675

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLKIDNGMAVNVEDIPIIPFEEFKDKILKFTETGYVVQHFAIPSYNINKNNSNENKYK
CCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEE
IYSVLRNDTELYVLSTMVGTSFESLSQYNIKFQMFEREIAEQYGIVPKNHPWLKSVRYHK
EEEECCCCCEEEEEEHHHCCCHHHHHHCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
NYKGAPDVFGNNYTAPIPGNYPFYEVGGEEIHQVAVGPVHAGIIEPGHFRFNCIGETILS
CCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCEEECCCCCCCEEEEEECCEEEE
LEIQHGYQHRGIEKQILNCKKEVLPLIIESIAGDTVIGNSICFSEAIEWLSNCNYEANEP
EEECCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCCCCCCC
LYNYRRLLLEIERIANHIGTMGGLAGDVGYIPTASYYGRIRGDFLNMFVLICGNRFGRSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCC
VRPFGAPFKLSDDHIKELIKRFEQLKKEVIDVGNLMLDNPEVLGRFDYTGIVDTKTAKLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHEECCCCCEECCCCEEEE
GLVGPAGRASGIKYDVRKSFSKAKNIYNRGIGLVARKDGAVSSRAKTYFYETLDSIEFCI
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ELLKVADFQINDSADKLEKIEKTVLKPNSFIITMEEAWRGELSHCIITDENGNIIRYKIK
HHHHHHCCEECCCHHHHHHHHHHHCCCCCEEEEECHHCCCCCCEEEEECCCCCEEEEEEC
DPSFHNWTALELVVRNEGIYDFPLCNKSFNLSYCGFDL
CCCCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GLLKIDNGMAVNVEDIPIIPFEEFKDKILKFTETGYVVQHFAIPSYNINKNNSNENKYK
CEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEE
IYSVLRNDTELYVLSTMVGTSFESLSQYNIKFQMFEREIAEQYGIVPKNHPWLKSVRYHK
EEEECCCCCEEEEEEHHHCCCHHHHHHCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
NYKGAPDVFGNNYTAPIPGNYPFYEVGGEEIHQVAVGPVHAGIIEPGHFRFNCIGETILS
CCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCEEECCCCCCCEEEEEECCEEEE
LEIQHGYQHRGIEKQILNCKKEVLPLIIESIAGDTVIGNSICFSEAIEWLSNCNYEANEP
EEECCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCCCCCCC
LYNYRRLLLEIERIANHIGTMGGLAGDVGYIPTASYYGRIRGDFLNMFVLICGNRFGRSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCC
VRPFGAPFKLSDDHIKELIKRFEQLKKEVIDVGNLMLDNPEVLGRFDYTGIVDTKTAKLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHEECCCCCEECCCCEEEE
GLVGPAGRASGIKYDVRKSFSKAKNIYNRGIGLVARKDGAVSSRAKTYFYETLDSIEFCI
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ELLKVADFQINDSADKLEKIEKTVLKPNSFIITMEEAWRGELSHCIITDENGNIIRYKIK
HHHHHHCCEECCCHHHHHHHHHHHCCCCCEEEEECHHCCCCCCEEEEECCCCCEEEEEEC
DPSFHNWTALELVVRNEGIYDFPLCNKSFNLSYCGFDL
CCCCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]