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The map label for this gene is bioD

Identifier: 150401311

GI number: 150401311

Start: 908081

End: 908719

Strand: Direct

Name: bioD

Synonym: Maeo_0883

Alternate gene names: 150401311

Gene position: 908081-908719 (Clockwise)

Preceding gene: 150401310

Following gene: 150401323

Centisome position: 57.86

GC content: 28.01

Gene sequence:

>639_bases
ATGATATTTATAACGGGAACTGACACAGGAGTAGGAAAAACCTTTGTTTCTAAAATAATCGGAAAACACTTAAAATACAA
AGAAAATATAAACACAGGATATTTAAAACCAATAGAATCGGGAGGTATCGAGGATACGGCAACCTTAAAAGAGGCTTTAA
ATTTAGATGAGAATTTAAATATATTAAATCCAGTGAATTTAAAAAACCCATTATCTCCGAATATTGCCGCTGAAATAGAA
AATAAACCAATAGATTTAGTAGAAATAAAAAAATCTTTTGATTTATTAAAAAATAAATATGATTTTTTGATTATTGAAGG
TGCTGGGGGGGTGGCAGTTCCAATAAAATACAATAGCTTTTTGATGTCTGATTTGATTGTTTATTTGGGAGCTCCTGCCA
TAATAGTATCAAGACCTGATTTAGGGACTATAAACCATACCCTTTTAACAATTGAACATTTAAGAAATAAAGGAATATTG
GTAAAAGGAGTTATAATAAACTGTATTAATGAGCTGGATACTGTTTTACATTACGAAAAAACTTTTGAAACGATAAATAG
ATGCGGAAATGTGGAAATTATTGGAATTGTTAAAAATGAAAATGAATATAACATTAATTTTGATAAAATAATTGGATAG

Upstream 100 bases:

>100_bases
CCATAAAAATACAGAAATACCGCCAAGTTATTTATTATTTTTATTTGGGCATAATATAGATATGGAAGTTAAAAATATAC
AATAAATATAATGTGATAAA

Downstream 100 bases:

>100_bases
AATTTTTTTAGGGAATGTCCTCAATATGTTTGTTATTAGAGCAATAAAAAATATCCTGCAATAATAATAGATGATACCAA
CGACAATATAAAACCTACTT

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIFITGTDTGVGKTFVSKIIGKHLKYKENINTGYLKPIESGGIEDTATLKEALNLDENLNILNPVNLKNPLSPNIAAEIE
NKPIDLVEIKKSFDLLKNKYDFLIIEGAGGVAVPIKYNSFLMSDLIVYLGAPAIIVSRPDLGTINHTLLTIEHLRNKGIL
VKGVIINCINELDTVLHYEKTFETINRCGNVEIIGIVKNENEYNINFDKIIG

Sequences:

>Translated_212_residues
MIFITGTDTGVGKTFVSKIIGKHLKYKENINTGYLKPIESGGIEDTATLKEALNLDENLNILNPVNLKNPLSPNIAAEIE
NKPIDLVEIKKSFDLLKNKYDFLIIEGAGGVAVPIKYNSFLMSDLIVYLGAPAIIVSRPDLGTINHTLLTIEHLRNKGIL
VKGVIINCINELDTVLHYEKTFETINRCGNVEIIGIVKNENEYNINFDKIIG
>Mature_212_residues
MIFITGTDTGVGKTFVSKIIGKHLKYKENINTGYLKPIESGGIEDTATLKEALNLDENLNILNPVNLKNPLSPNIAAEIE
NKPIDLVEIKKSFDLLKNKYDFLIIEGAGGVAVPIKYNSFLMSDLIVYLGAPAIIVSRPDLGTINHTLLTIEHLRNKGIL
VKGVIINCINELDTVLHYEKTFETINRCGNVEIIGIVKNENEYNINFDKIIG

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=173, Percent_Identity=31.7919075144509, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87081944, Length=202, Percent_Identity=31.1881188118812, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324385, Length=182, Percent_Identity=36.2637362637363, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_META3 (A6UVE3)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325077.1
- ProteinModelPortal:   A6UVE3
- SMR:   A6UVE3
- STRING:   A6UVE3
- GeneID:   5326991
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0883
- eggNOG:   arNOG09163
- HOGENOM:   HBG650065
- OMA:   LNNYNHE
- ProtClustDB:   CLSK876518
- BioCyc:   MAEO419665:MAEO_0883-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 23478; Mature: 23478

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFITGTDTGVGKTFVSKIIGKHLKYKENINTGYLKPIESGGIEDTATLKEALNLDENLN
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
ILNPVNLKNPLSPNIAAEIENKPIDLVEIKKSFDLLKNKYDFLIIEGAGGVAVPIKYNSF
EECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECHH
LMSDLIVYLGAPAIIVSRPDLGTINHTLLTIEHLRNKGILVKGVIINCINELDTVLHYEK
HHHHHHHHHCCCEEEEECCCCCCCHHHEEEEEHHCCCCEEEEHHHHHHHHHHHHHHHHHH
TFETINRCGNVEIIGIVKNENEYNINFDKIIG
HHHHHHHCCCEEEEEEEECCCCCCCCHHHHCC
>Mature Secondary Structure
MIFITGTDTGVGKTFVSKIIGKHLKYKENINTGYLKPIESGGIEDTATLKEALNLDENLN
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
ILNPVNLKNPLSPNIAAEIENKPIDLVEIKKSFDLLKNKYDFLIIEGAGGVAVPIKYNSF
EECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECHH
LMSDLIVYLGAPAIIVSRPDLGTINHTLLTIEHLRNKGILVKGVIINCINELDTVLHYEK
HHHHHHHHHCCCEEEEECCCCCCCHHHEEEEEHHCCCCEEEEHHHHHHHHHHHHHHHHHH
TFETINRCGNVEIIGIVKNENEYNINFDKIIG
HHHHHHHCCCEEEEEEEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA