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The map label for this gene is tgtA

Identifier: 150401306

GI number: 150401306

Start: 903715

End: 905673

Strand: Direct

Name: tgtA

Synonym: Maeo_0878

Alternate gene names: 150401306

Gene position: 903715-905673 (Clockwise)

Preceding gene: 150401305

Following gene: 150401307

Centisome position: 57.58

GC content: 29.5

Gene sequence:

>1959_bases
ATGTTTGAAATAAAACACAGAGATGCCATGGGCAGAATCGGAAAATTAAAAATAAATGGAAAAATAATAGAAACTCCAAC
AATTATGCCTGTTGTTCATCCAAATCCAAAAAAACAGACTGTTTCAATAGAAAAAATTGAAAAATTAGCATCCGTAATTA
TAACCAATTCATATATTATTAATACCAATCCAGAATTAAAAAAAATAGCAGAAGAAAAAGGAATTCATAATTTAATAGGA
TTTGATAAGGTAATAGTTACAGATAGTGGTTCATTCCAATTAAGTGTATATGGAGAAATTACCGTTGAACCAGAAGAAAT
AATTGAATTTCAAGAAAAAATCGGCGTAGATGTTGGAACAATATTGGACATTCCAACAGCACCATATGTACAGAGAGAAC
AGGCCGAAGAAGAATTAAAAGAAACTCTTAGAAGAGCAGAATTATCTGTAAAGTTAAGAGATGAAAAAAATTATAAATTA
TTATTAAATGGAACTATTCAAGGTTCTACCTACATGGATTTAAGGCAGGAAAGTGCTAAAAAAATGAAGGAATTGGATTT
TGATATATATCCAATTGGGGCTGTTGTTCCATTAATGGAAAATTATGATTATGCTCAAGTTATTGAAATAATATTAAATT
CTAAAAAGGAGCTCCCTACAAATAAACCTGTGCATTTGTTTGGTTGCGGACATCCTATGATGTTTGCCCTATCTGTGGCA
TTAGGATGCGATTTATTTGACAGTGCTGCTTATGCATTATATGCAAAAGATGGACGATATTTAACAGAATCAGGCACCCT
TCATTTAGAGGATTTAAAAGATTTAGAAAAATTCCCATGTTCATGTCCTGTATGTATTGAATATACTCCAAAGGAATTGG
CAAAAATGGAAAAACGCAAAAGAACTGAATTATTAGCCGAACACAATTTATACATTACATTTGAGGAAATTAACAGAATT
AAACAGGCAATTAAAGACGGTAATTTATGGGAGCTTGTAGAGGAAAGATGTAGGGCACATCCAAAATTATTGGAGGCATA
TAGAAAAGCATTAGAATATAATGAATTTATCGAAGAATTCGACCCCATTACAAAAAAATCTGCCTTCTTTTACGGAGGAT
ATGAAAGTTTATATCGTCCAGAAATTATAAGGCATAAAAAACGATTAGAAAGAATAAAATCAGACAATATATATATTACA
ACAATATCGAACGATATTAATAGACCATACAGCGAAAATACAAATATGATGGATAGCGATGCACTGGTCCTTATTAAAGA
TGATTTATTTGGATTAGTGCCTTTATCAATTGATACAGTTTATCCACTGTCCCAATGCGATAATCCAAAGTTATATTGTT
ATGAAAAAATACATAACAATGAGTTTGTTGAGGAATTTAAACAAAATAACAAAGATAAAATAATGGATATAACTTCATAT
AATTACTATATAAATCATTATACTTCCAACGCCAACAAAAATAAAGTAGGTAGTGACGCCATCAGAATAAACAATATGAT
TCAATATCAATATGGATTTAAATTAAATGATGAGGAATTAAAAAAATTAATAATAAAAAGAAGTAAAAAAACAAATAGAA
TTAGAAATGTCCTTATACCAACAGATGGAAACAACAAAGAAGTATTATTTACACTTCGTTCAAATGATAATTTATTAATT
CCTGCAAAAGAGGGGGCAAAATTAATTCATGAAAAATTACCATTTCCAAAATATAGGGTTGTAATTGATAGCGAAGTTGA
GGAATTTGCAAGAGATGGAAAATCCGTATTTTCTAAATTTGTAATAAACTGCGATAAGGAATTAAGGCCTTATGAGGAAG
TAATTATTGTAAATGAAAATGATGAATTACTTGCCTATGGAACAACATTATTAAATGGTAAAGAATTAGGGGAATTTAAT
TATGGTGCTGCTGTTAGTGTAAGAAGTGGATTTAAATGA

Upstream 100 bases:

>100_bases
AAAAATCCATTCAAGATTTTTATATGGGGTCATGGGCAGTAGCAAAATCTAATTTTATAATTCCACTAAAAACATACCAT
ATATAAAAAACGGTGAAATA

Downstream 100 bases:

>100_bases
TGCAACCGTATATAATATATATCATATAAATCTAATATTACATATTTAATTTTTGTTAATTTTTTGAACTATTGATGCAT
ATTGGCGGGAGATTATGAAT

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MFEIKHRDAMGRIGKLKINGKIIETPTIMPVVHPNPKKQTVSIEKIEKLASVIITNSYIINTNPELKKIAEEKGIHNLIG
FDKVIVTDSGSFQLSVYGEITVEPEEIIEFQEKIGVDVGTILDIPTAPYVQREQAEEELKETLRRAELSVKLRDEKNYKL
LLNGTIQGSTYMDLRQESAKKMKELDFDIYPIGAVVPLMENYDYAQVIEIILNSKKELPTNKPVHLFGCGHPMMFALSVA
LGCDLFDSAAYALYAKDGRYLTESGTLHLEDLKDLEKFPCSCPVCIEYTPKELAKMEKRKRTELLAEHNLYITFEEINRI
KQAIKDGNLWELVEERCRAHPKLLEAYRKALEYNEFIEEFDPITKKSAFFYGGYESLYRPEIIRHKKRLERIKSDNIYIT
TISNDINRPYSENTNMMDSDALVLIKDDLFGLVPLSIDTVYPLSQCDNPKLYCYEKIHNNEFVEEFKQNNKDKIMDITSY
NYYINHYTSNANKNKVGSDAIRINNMIQYQYGFKLNDEELKKLIIKRSKKTNRIRNVLIPTDGNNKEVLFTLRSNDNLLI
PAKEGAKLIHEKLPFPKYRVVIDSEVEEFARDGKSVFSKFVINCDKELRPYEEVIIVNENDELLAYGTTLLNGKELGEFN
YGAAVSVRSGFK

Sequences:

>Translated_652_residues
MFEIKHRDAMGRIGKLKINGKIIETPTIMPVVHPNPKKQTVSIEKIEKLASVIITNSYIINTNPELKKIAEEKGIHNLIG
FDKVIVTDSGSFQLSVYGEITVEPEEIIEFQEKIGVDVGTILDIPTAPYVQREQAEEELKETLRRAELSVKLRDEKNYKL
LLNGTIQGSTYMDLRQESAKKMKELDFDIYPIGAVVPLMENYDYAQVIEIILNSKKELPTNKPVHLFGCGHPMMFALSVA
LGCDLFDSAAYALYAKDGRYLTESGTLHLEDLKDLEKFPCSCPVCIEYTPKELAKMEKRKRTELLAEHNLYITFEEINRI
KQAIKDGNLWELVEERCRAHPKLLEAYRKALEYNEFIEEFDPITKKSAFFYGGYESLYRPEIIRHKKRLERIKSDNIYIT
TISNDINRPYSENTNMMDSDALVLIKDDLFGLVPLSIDTVYPLSQCDNPKLYCYEKIHNNEFVEEFKQNNKDKIMDITSY
NYYINHYTSNANKNKVGSDAIRINNMIQYQYGFKLNDEELKKLIIKRSKKTNRIRNVLIPTDGNNKEVLFTLRSNDNLLI
PAKEGAKLIHEKLPFPKYRVVIDSEVEEFARDGKSVFSKFVINCDKELRPYEEVIIVNENDELLAYGTTLLNGKELGEFN
YGAAVSVRSGFK
>Mature_652_residues
MFEIKHRDAMGRIGKLKINGKIIETPTIMPVVHPNPKKQTVSIEKIEKLASVIITNSYIINTNPELKKIAEEKGIHNLIG
FDKVIVTDSGSFQLSVYGEITVEPEEIIEFQEKIGVDVGTILDIPTAPYVQREQAEEELKETLRRAELSVKLRDEKNYKL
LLNGTIQGSTYMDLRQESAKKMKELDFDIYPIGAVVPLMENYDYAQVIEIILNSKKELPTNKPVHLFGCGHPMMFALSVA
LGCDLFDSAAYALYAKDGRYLTESGTLHLEDLKDLEKFPCSCPVCIEYTPKELAKMEKRKRTELLAEHNLYITFEEINRI
KQAIKDGNLWELVEERCRAHPKLLEAYRKALEYNEFIEEFDPITKKSAFFYGGYESLYRPEIIRHKKRLERIKSDNIYIT
TISNDINRPYSENTNMMDSDALVLIKDDLFGLVPLSIDTVYPLSQCDNPKLYCYEKIHNNEFVEEFKQNNKDKIMDITSY
NYYINHYTSNANKNKVGSDAIRINNMIQYQYGFKLNDEELKKLIIKRSKKTNRIRNVLIPTDGNNKEVLFTLRSNDNLLI
PAKEGAKLIHEKLPFPKYRVVIDSEVEEFARDGKSVFSKFVINCDKELRPYEEVIIVNENDELLAYGTTLLNGKELGEFN
YGAAVSVRSGFK

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI13654276, Length=342, Percent_Identity=23.9766081871345, Blast_Score=105, Evalue=2e-22,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=25.8953168044077, Blast_Score=119, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17544680, Length=365, Percent_Identity=22.7397260273973, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920510, Length=341, Percent_Identity=24.3401759530792, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_META3 (A6UVD8)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325072.1
- ProteinModelPortal:   A6UVD8
- SMR:   A6UVD8
- STRING:   A6UVD8
- GeneID:   5326446
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0878
- eggNOG:   arNOG04378
- HOGENOM:   HBG512800
- OMA:   EERCRAH
- ProtClustDB:   PRK13534
- BioCyc:   MAEO419665:MAEO_0878-MONOMER
- HAMAP:   MF_01634
- InterPro:   IPR004804
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR002616
- InterPro:   IPR004521
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- SMART:   SM00359
- TIGRFAMs:   TIGR00432
- TIGRFAMs:   TIGR00449
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF01472 PUA; PF01702 TGT; SSF88697 PUA-like; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 75132; Mature: 75132

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 88-88

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEIKHRDAMGRIGKLKINGKIIETPTIMPVVHPNPKKQTVSIEKIEKLASVIITNSYII
CCEECCCHHCCCCEEEEECCEEEECCCEEEEECCCCCCCEEEHHHHHHHHHHHEECCEEE
NTNPELKKIAEEKGIHNLIGFDKVIVTDSGSFQLSVYGEITVEPEEIIEFQEKIGVDVGT
CCCHHHHHHHHHCCCHHHCCCCEEEEECCCCEEEEEEEEEEECHHHHHHHHHHHCCCCHH
ILDIPTAPYVQREQAEEELKETLRRAELSVKLRDEKNYKLLLNGTIQGSTYMDLRQESAK
EEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHH
KMKELDFDIYPIGAVVPLMENYDYAQVIEIILNSKKELPTNKPVHLFGCGHPMMFALSVA
HHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHH
LGCDLFDSAAYALYAKDGRYLTESGTLHLEDLKDLEKFPCSCPVCIEYTPKELAKMEKRK
HCCCHHCCCEEEEEECCCCEEECCCCEEHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
RTELLAEHNLYITFEEINRIKQAIKDGNLWELVEERCRAHPKLLEAYRKALEYNEFIEEF
HHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DPITKKSAFFYGGYESLYRPEIIRHKKRLERIKSDNIYITTISNDINRPYSENTNMMDSD
CCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
ALVLIKDDLFGLVPLSIDTVYPLSQCDNPKLYCYEKIHNNEFVEEFKQNNKDKIMDITSY
CEEEEECCCCEEEEEEECCCCCHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEE
NYYINHYTSNANKNKVGSDAIRINNMIQYQYGFKLNDEELKKLIIKRSKKTNRIRNVLIP
EEEEEEEECCCCCCCCCCCCEEECCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEE
TDGNNKEVLFTLRSNDNLLIPAKEGAKLIHEKLPFPKYRVVIDSEVEEFARDGKSVFSKF
CCCCCCEEEEEEECCCCEEEECCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHH
VINCDKELRPYEEVIIVNENDELLAYGTTLLNGKELGEFNYGAAVSVRSGFK
HHCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MFEIKHRDAMGRIGKLKINGKIIETPTIMPVVHPNPKKQTVSIEKIEKLASVIITNSYII
CCEECCCHHCCCCEEEEECCEEEECCCEEEEECCCCCCCEEEHHHHHHHHHHHEECCEEE
NTNPELKKIAEEKGIHNLIGFDKVIVTDSGSFQLSVYGEITVEPEEIIEFQEKIGVDVGT
CCCHHHHHHHHHCCCHHHCCCCEEEEECCCCEEEEEEEEEEECHHHHHHHHHHHCCCCHH
ILDIPTAPYVQREQAEEELKETLRRAELSVKLRDEKNYKLLLNGTIQGSTYMDLRQESAK
EEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHH
KMKELDFDIYPIGAVVPLMENYDYAQVIEIILNSKKELPTNKPVHLFGCGHPMMFALSVA
HHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHH
LGCDLFDSAAYALYAKDGRYLTESGTLHLEDLKDLEKFPCSCPVCIEYTPKELAKMEKRK
HCCCHHCCCEEEEEECCCCEEECCCCEEHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
RTELLAEHNLYITFEEINRIKQAIKDGNLWELVEERCRAHPKLLEAYRKALEYNEFIEEF
HHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DPITKKSAFFYGGYESLYRPEIIRHKKRLERIKSDNIYITTISNDINRPYSENTNMMDSD
CCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
ALVLIKDDLFGLVPLSIDTVYPLSQCDNPKLYCYEKIHNNEFVEEFKQNNKDKIMDITSY
CEEEEECCCCEEEEEEECCCCCHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEE
NYYINHYTSNANKNKVGSDAIRINNMIQYQYGFKLNDEELKKLIIKRSKKTNRIRNVLIP
EEEEEEEECCCCCCCCCCCCEEECCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEE
TDGNNKEVLFTLRSNDNLLIPAKEGAKLIHEKLPFPKYRVVIDSEVEEFARDGKSVFSKF
CCCCCCEEEEEEECCCCEEEECCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHH
VINCDKELRPYEEVIIVNENDELLAYGTTLLNGKELGEFNYGAAVSVRSGFK
HHCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA