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The map label for this gene is rpoB2 [H]

Identifier: 150401220

GI number: 150401220

Start: 817911

End: 819443

Strand: Reverse

Name: rpoB2 [H]

Synonym: Maeo_0791

Alternate gene names: 150401220

Gene position: 819443-817911 (Counterclockwise)

Preceding gene: 150401221

Following gene: 150401219

Centisome position: 52.21

GC content: 35.62

Gene sequence:

>1533_bases
ATGGAATCCCGTATAATTGTTGACGAATTTTTTAAAGAAAATAGTTTGGTTGAACACCATATTAACTCATATAATAAATT
TGTGGAAAATAGCATCCAAAAAGTTATCGACGAAGCAGCTCCTATTGAGGCAGAAGTAAAAGGAGGATTCAAAGTAAAAT
TTGGAAAAGTTAGAGTAGGAAAACCGATAAATAAAGAAGCAGACGGCTCTGTTAAGGAAATTTATCCAGTTGAGGCAAGA
ATTAGAAATTTATCCTACTCGGCCCCCTTATATTTGGAAATTACTCCTATAATGGGCGGAGGTGAAGATGAAGAAGGTAA
TAAATATGAAGTAGAAGAGGGAGAAACTGTTGAGGCCTATATTGGGGAGCTCCCTGTCATGCTTGGTTCCATTATATGTC
ATTTAAATGGTAAGTCCGAAGATGAATTGGTAAAAATGGGGGAAGACGCAAGAGACCCTATGGGCTATTTTATTGTTAAT
GGTTCAGAAAAGGCAGTTGTAGCACAGGAGGATTTAATTCCAAATAGAATATTGTGTGAGAAAGTAGAAAAAACAGGAAA
ACCAGTTTATATTGCAAAAGTATTTTCAACAAGACATGGATTTAGGGCATTATGTGCTGTGGAAAAACACGACGATGGAA
GATTAAATGTGTCCTTCCCAGGAATGTCTGGAAATATTCCACTTGTGATATTGTTAAAAGCATTAGGTGCTGAAAATGAT
AGTGCAGTATTTAAAATAATTTCGGAAGACCCTGCTGTTAGTATGCAGTTGATAGCAAACATACTAGAAACAAGAGAGGA
ACATCAAGTAATAACCCGAGAAGATGCTCTGGAATATATCGGAAAAAGAGTGGCCCCAGGTCAGCCAAAAGAATATAGGC
TTAAAAGAGCTGAAACTATACTTTGTAACTATTTATTGCCACATATGGGCGTTAAAAAGGAAAATTTGTTTGAAAAATGT
ATTTATTTAGGTAGAATGGCCAAAAATGCCATAGAATTATATTTGGGATTGAGGGTTGAAGATGATAAGGACCACTATGC
AAATAAAAGATTAAAATTAGCAGGGGACCTTATGGAAGACCTTTTTAGGTATTCATTTAACCAATTGAACAAAGATATGA
AATACCAATTAGAGAGACAGGCAATAAGAAATAAATCTCCATCAATTCAAGCCGCAGTTAGAAGTGATGTTTTAACTGAA
AGAATAAGACATGCTATGGCAACAGGAAACTGGGTTGGCGGTAGAACTGGAGTTAGTCAATTACTTGATAGAACAAGTTA
TTTGGCAACAGTTTCGCAACTTAGAAGATTGGTATCTCCTTTATCTAGGTCACAGCCACACTTTGAAGCAAGGGATTTAC
ATGCTACACAGTGGGGAAAAATTTGTCCATCAGAAACTCCTGAGGGTCCAAACTGTGGTTTGGTTAAAAACATAGCAGTA
ATGTGTGAAATCACTACACAGGAACAAGATGATAAAATAATTGATTTATTAAAAGAATCAGGATATGTAAAATCGGCTAA
GGATAGTTATTAA

Upstream 100 bases:

>100_bases
TTTCTTGTATTGAATTATTAATTATCGTTCTATATATTTAGCATACTAATAAGCATATTTATATTTATATTTATAATAAT
TTTAAATAAGGGGGAAAATC

Downstream 100 bases:

>100_bases
TCTATATTTTAAATAATATATATTTAAATTTTTAATTATATTTAATGATAATTATTACTATTATATTAATTGTCATATTC
AATAAAATTTATTAGGTTAA

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MESRIIVDEFFKENSLVEHHINSYNKFVENSIQKVIDEAAPIEAEVKGGFKVKFGKVRVGKPINKEADGSVKEIYPVEAR
IRNLSYSAPLYLEITPIMGGGEDEEGNKYEVEEGETVEAYIGELPVMLGSIICHLNGKSEDELVKMGEDARDPMGYFIVN
GSEKAVVAQEDLIPNRILCEKVEKTGKPVYIAKVFSTRHGFRALCAVEKHDDGRLNVSFPGMSGNIPLVILLKALGAEND
SAVFKIISEDPAVSMQLIANILETREEHQVITREDALEYIGKRVAPGQPKEYRLKRAETILCNYLLPHMGVKKENLFEKC
IYLGRMAKNAIELYLGLRVEDDKDHYANKRLKLAGDLMEDLFRYSFNQLNKDMKYQLERQAIRNKSPSIQAAVRSDVLTE
RIRHAMATGNWVGGRTGVSQLLDRTSYLATVSQLRRLVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNIAV
MCEITTQEQDDKIIDLLKESGYVKSAKDSY

Sequences:

>Translated_510_residues
MESRIIVDEFFKENSLVEHHINSYNKFVENSIQKVIDEAAPIEAEVKGGFKVKFGKVRVGKPINKEADGSVKEIYPVEAR
IRNLSYSAPLYLEITPIMGGGEDEEGNKYEVEEGETVEAYIGELPVMLGSIICHLNGKSEDELVKMGEDARDPMGYFIVN
GSEKAVVAQEDLIPNRILCEKVEKTGKPVYIAKVFSTRHGFRALCAVEKHDDGRLNVSFPGMSGNIPLVILLKALGAEND
SAVFKIISEDPAVSMQLIANILETREEHQVITREDALEYIGKRVAPGQPKEYRLKRAETILCNYLLPHMGVKKENLFEKC
IYLGRMAKNAIELYLGLRVEDDKDHYANKRLKLAGDLMEDLFRYSFNQLNKDMKYQLERQAIRNKSPSIQAAVRSDVLTE
RIRHAMATGNWVGGRTGVSQLLDRTSYLATVSQLRRLVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNIAV
MCEITTQEQDDKIIDLLKESGYVKSAKDSY
>Mature_510_residues
MESRIIVDEFFKENSLVEHHINSYNKFVENSIQKVIDEAAPIEAEVKGGFKVKFGKVRVGKPINKEADGSVKEIYPVEAR
IRNLSYSAPLYLEITPIMGGGEDEEGNKYEVEEGETVEAYIGELPVMLGSIICHLNGKSEDELVKMGEDARDPMGYFIVN
GSEKAVVAQEDLIPNRILCEKVEKTGKPVYIAKVFSTRHGFRALCAVEKHDDGRLNVSFPGMSGNIPLVILLKALGAEND
SAVFKIISEDPAVSMQLIANILETREEHQVITREDALEYIGKRVAPGQPKEYRLKRAETILCNYLLPHMGVKKENLFEKC
IYLGRMAKNAIELYLGLRVEDDKDHYANKRLKLAGDLMEDLFRYSFNQLNKDMKYQLERQAIRNKSPSIQAAVRSDVLTE
RIRHAMATGNWVGGRTGVSQLLDRTSYLATVSQLRRLVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNIAV
MCEITTQEQDDKIIDLLKESGYVKSAKDSY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=533, Percent_Identity=34.3339587242026, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI238908503, Length=511, Percent_Identity=34.2465753424658, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI238908505, Length=484, Percent_Identity=34.7107438016529, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI33469941, Length=518, Percent_Identity=24.1312741312741, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI212286172, Length=414, Percent_Identity=24.6376811594203, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1790419, Length=147, Percent_Identity=29.2517006802721, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17552304, Length=542, Percent_Identity=31.9188191881919, Blast_Score=287, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI25144348, Length=523, Percent_Identity=31.9311663479924, Blast_Score=254, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI17506623, Length=524, Percent_Identity=25.1908396946565, Blast_Score=122, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324725, Length=538, Percent_Identity=33.8289962825279, Blast_Score=289, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6324781, Length=520, Percent_Identity=30.5769230769231, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6325267, Length=529, Percent_Identity=25.5198487712665, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17136444, Length=530, Percent_Identity=34.3396226415094, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI17647877, Length=508, Percent_Identity=31.1023622047244, Blast_Score=247, Evalue=1e-65,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 57342; Mature: 57342

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESRIIVDEFFKENSLVEHHINSYNKFVENSIQKVIDEAAPIEAEVKGGFKVKFGKVRVG
CCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECEEEEC
KPINKEADGSVKEIYPVEARIRNLSYSAPLYLEITPIMGGGEDEEGNKYEVEEGETVEAY
CCCCCCCCCCHHHEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHH
IGELPVMLGSIICHLNGKSEDELVKMGEDARDPMGYFIVNGSEKAVVAQEDLIPNRILCE
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEHHHCCCHHHHHH
KVEKTGKPVYIAKVFSTRHGFRALCAVEKHDDGRLNVSFPGMSGNIPLVILLKALGAEND
HHHHCCCCEEEEEEHHCCCCHHEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCC
SAVFKIISEDPAVSMQLIANILETREEHQVITREDALEYIGKRVAPGQPKEYRLKRAETI
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LCNYLLPHMGVKKENLFEKCIYLGRMAKNAIELYLGLRVEDDKDHYANKRLKLAGDLMED
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECEEECCCHHHHCCHHHHHHHHHHH
LFRYSFNQLNKDMKYQLERQAIRNKSPSIQAAVRSDVLTERIRHAMATGNWVGGRTGVSQ
HHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LLDRTSYLATVSQLRRLVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNIAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHCEEE
MCEITTQEQDDKIIDLLKESGYVKSAKDSY
EEEEECCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MESRIIVDEFFKENSLVEHHINSYNKFVENSIQKVIDEAAPIEAEVKGGFKVKFGKVRVG
CCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECEEEEC
KPINKEADGSVKEIYPVEARIRNLSYSAPLYLEITPIMGGGEDEEGNKYEVEEGETVEAY
CCCCCCCCCCHHHEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHH
IGELPVMLGSIICHLNGKSEDELVKMGEDARDPMGYFIVNGSEKAVVAQEDLIPNRILCE
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEHHHCCCHHHHHH
KVEKTGKPVYIAKVFSTRHGFRALCAVEKHDDGRLNVSFPGMSGNIPLVILLKALGAEND
HHHHCCCCEEEEEEHHCCCCHHEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCC
SAVFKIISEDPAVSMQLIANILETREEHQVITREDALEYIGKRVAPGQPKEYRLKRAETI
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LCNYLLPHMGVKKENLFEKCIYLGRMAKNAIELYLGLRVEDDKDHYANKRLKLAGDLMED
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECEEECCCHHHHCCHHHHHHHHHHH
LFRYSFNQLNKDMKYQLERQAIRNKSPSIQAAVRSDVLTERIRHAMATGNWVGGRTGVSQ
HHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LLDRTSYLATVSQLRRLVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNIAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHCEEE
MCEITTQEQDDKIIDLLKESGYVKSAKDSY
EEEEECCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA