Click here to switch to the map view.

The map label for this gene is rpoB1 [H]

Identifier: 150401219

GI number: 150401219

Start: 815962

End: 817797

Strand: Reverse

Name: rpoB1 [H]

Synonym: Maeo_0790

Alternate gene names: 150401219

Gene position: 817797-815962 (Counterclockwise)

Preceding gene: 150401220

Following gene: 150401218

Centisome position: 52.11

GC content: 37.25

Gene sequence:

>1836_bases
TTGGAAAAATATGCAAATTTATATGTTAATGGAAAATTAATCGGTTCAGTGGAGTCTCCCGATGAACTTATAAAATATTT
AAGGGATAATAGGCGAAATGGCCAAATATCTCCTTTTACTTCAATATCTTACAATGAAGAGAGCTCCGATATACACATTT
CAACGGATGCAGGTAGGGCCGTTAGGCCATTAATTGTTGTAGAAAATGGAGTGTCTAAATTAAACGAAGAACTCATTAAA
AAAGTAGAGCAGGGCGAACTTAATTTTTCAGGATTGGTTAAAATGGGGGTTATTGAATATTTAGATGCCGAGGAAGAGGA
GAATGCATATATTGCAAACTATGAAAAAGATTTAACTAAAAATCACACTCATCTTGAAATTGACCCATTATCAATACTTG
GAATAGGTGCAGGTGTTGCCCCATATCCTGAGCATAACTCTGCTCCAAGGATTACAATGGCGGCGGCCATGGGTAAGCAA
TCCCTTGGAATGCCATTGGCAAATATGAAATTAAGAATGGATACAAAGGCACATTTATTACATTATCCTCAGGTTCCTAT
TGTTAGAACTAAACACCAAGAAATACTTGGATTTGACAGAAGACCAGCAGGTCAAAATTTTGTTGTAGCTGTAATGAGTT
ATGAAGGATACAACATGGAGGATGCTTTTATCATAAATAAGGCATCAATCGAAAGAGGACTTGCGAGAAGTAGCTTTTTC
AGAAGCTATGAAGGTTTTGAAAAAAGACATCCTGGGGGACAGGTAGATAGGTTTGAAATCCCTGAGCAAGGGGTTAGAGG
ATACAGGGCAGAAGAGGCATATAGAAATTTAGGGGACGATGGTATTGTAGAGGTAGAATCAGAGGTAAAATCTGGAGATG
TAATAATTGGAAAAACCTCCCCACCAAGATTTTTGGAGGAGCAAGATATATCTTTAAGGTCTAAATCTCAAAGAAGAGAT
ACCTCTATTACCATTAGACATGGAGAAGGGGGCATTGTAGATGCCGTAATATTGTCTGAAACCAAAGAAGGAAATAGATT
GGCAAAAATTAGAGTAAGAGATTTAAGAATACCAGAATTGGGAGATAAATTTGCTTCAAGGCACGGTCAGAAAGGGGTTT
TAGGTCTTATAGTTCCCCAAGAAGACATGCCATTTACTGAAAATGGAGTAGTTCCAGATTTAATAATTAATCCCCATGCA
ATTCCATCAAGGATGACTATTGGTCAGGTATTGGAAATGATTGGGGGAAAAGTCGGTTCTATGAACTGTAAAAGAATAGA
TGGAACTATATTTAGTGGAGACCCAGAATGGGAATTAAGGAGCTCCATGGAAAAGTGCGGATACAAACACAATGGTAAAG
AAGTAATGTTTGATGGAAAAACTGGAAAAAAACTTGAATGCGAAATATTTGTTGGAATAGCATACTACCAGAAATTACAT
CATCTTGTGGCAGGAAAAATGCATGCAAGAAGTAGGGGTCCAATACAGGTATTAACAAGACAGCCAACAGAAGGTAGGGC
AAGAGAAGGAGGTTTAAGATTTGGAGAAATGGAAAGGGATGTTCTTATCGGGCATGGAACGGCATTATTATTGAAGGAAC
GATTATTTGATGAATCCGACCCTTATGAATGCCATATTTGTTCCAACTGTGGTGAAGTTGCCATATATGACCACAAAAGA
GGAATGAAAAGATGTCAAGCATGTGGCGAAACAGAGGATGTTCAAAATAATAGAAAAATACCTTCTGTAAGGTTGCCTTA
TGCCTTTAAGTTATTATTGGATGAGCTTAGAAGTATGGGAATAAATCCAAAGCTTGAATTAAAAGATAGAGTATAA

Upstream 100 bases:

>100_bases
TAATATATATTTAAATTTTTAATTATATTTAATGATAATTATTACTATTATATTAATTGTCATATTCAATAAAATTTATT
AGGTTAAAAAGGTGAAAACC

Downstream 100 bases:

>100_bases
TTATATTTTTAATTATATTCTTAATATTTATTATGTATGGTCAATCTTCGGTATTTTTCACCAAACTGTTCTAGATTAAA
AGCTAAAATCTCTCCATATT

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MEKYANLYVNGKLIGSVESPDELIKYLRDNRRNGQISPFTSISYNEESSDIHISTDAGRAVRPLIVVENGVSKLNEELIK
KVEQGELNFSGLVKMGVIEYLDAEEEENAYIANYEKDLTKNHTHLEIDPLSILGIGAGVAPYPEHNSAPRITMAAAMGKQ
SLGMPLANMKLRMDTKAHLLHYPQVPIVRTKHQEILGFDRRPAGQNFVVAVMSYEGYNMEDAFIINKASIERGLARSSFF
RSYEGFEKRHPGGQVDRFEIPEQGVRGYRAEEAYRNLGDDGIVEVESEVKSGDVIIGKTSPPRFLEEQDISLRSKSQRRD
TSITIRHGEGGIVDAVILSETKEGNRLAKIRVRDLRIPELGDKFASRHGQKGVLGLIVPQEDMPFTENGVVPDLIINPHA
IPSRMTIGQVLEMIGGKVGSMNCKRIDGTIFSGDPEWELRSSMEKCGYKHNGKEVMFDGKTGKKLECEIFVGIAYYQKLH
HLVAGKMHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLIGHGTALLLKERLFDESDPYECHICSNCGEVAIYDHKR
GMKRCQACGETEDVQNNRKIPSVRLPYAFKLLLDELRSMGINPKLELKDRV

Sequences:

>Translated_611_residues
MEKYANLYVNGKLIGSVESPDELIKYLRDNRRNGQISPFTSISYNEESSDIHISTDAGRAVRPLIVVENGVSKLNEELIK
KVEQGELNFSGLVKMGVIEYLDAEEEENAYIANYEKDLTKNHTHLEIDPLSILGIGAGVAPYPEHNSAPRITMAAAMGKQ
SLGMPLANMKLRMDTKAHLLHYPQVPIVRTKHQEILGFDRRPAGQNFVVAVMSYEGYNMEDAFIINKASIERGLARSSFF
RSYEGFEKRHPGGQVDRFEIPEQGVRGYRAEEAYRNLGDDGIVEVESEVKSGDVIIGKTSPPRFLEEQDISLRSKSQRRD
TSITIRHGEGGIVDAVILSETKEGNRLAKIRVRDLRIPELGDKFASRHGQKGVLGLIVPQEDMPFTENGVVPDLIINPHA
IPSRMTIGQVLEMIGGKVGSMNCKRIDGTIFSGDPEWELRSSMEKCGYKHNGKEVMFDGKTGKKLECEIFVGIAYYQKLH
HLVAGKMHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLIGHGTALLLKERLFDESDPYECHICSNCGEVAIYDHKR
GMKRCQACGETEDVQNNRKIPSVRLPYAFKLLLDELRSMGINPKLELKDRV
>Mature_611_residues
MEKYANLYVNGKLIGSVESPDELIKYLRDNRRNGQISPFTSISYNEESSDIHISTDAGRAVRPLIVVENGVSKLNEELIK
KVEQGELNFSGLVKMGVIEYLDAEEEENAYIANYEKDLTKNHTHLEIDPLSILGIGAGVAPYPEHNSAPRITMAAAMGKQ
SLGMPLANMKLRMDTKAHLLHYPQVPIVRTKHQEILGFDRRPAGQNFVVAVMSYEGYNMEDAFIINKASIERGLARSSFF
RSYEGFEKRHPGGQVDRFEIPEQGVRGYRAEEAYRNLGDDGIVEVESEVKSGDVIIGKTSPPRFLEEQDISLRSKSQRRD
TSITIRHGEGGIVDAVILSETKEGNRLAKIRVRDLRIPELGDKFASRHGQKGVLGLIVPQEDMPFTENGVVPDLIINPHA
IPSRMTIGQVLEMIGGKVGSMNCKRIDGTIFSGDPEWELRSSMEKCGYKHNGKEVMFDGKTGKKLECEIFVGIAYYQKLH
HLVAGKMHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLIGHGTALLLKERLFDESDPYECHICSNCGEVAIYDHKR
GMKRCQACGETEDVQNNRKIPSVRLPYAFKLLLDELRSMGINPKLELKDRV

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=617, Percent_Identity=44.4084278768233, Blast_Score=510, Evalue=1e-144,
Organism=Homo sapiens, GI238908505, Length=604, Percent_Identity=41.5562913907285, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI238908503, Length=604, Percent_Identity=41.5562913907285, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI212286172, Length=576, Percent_Identity=35.0694444444444, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI33469941, Length=576, Percent_Identity=34.8958333333333, Blast_Score=311, Evalue=8e-85,
Organism=Escherichia coli, GI1790419, Length=300, Percent_Identity=33, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17552304, Length=617, Percent_Identity=44.4084278768233, Blast_Score=518, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI25144348, Length=610, Percent_Identity=41.3114754098361, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17506623, Length=650, Percent_Identity=31.3846153846154, Blast_Score=290, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6324781, Length=610, Percent_Identity=43.9344262295082, Blast_Score=519, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324725, Length=645, Percent_Identity=42.6356589147287, Blast_Score=503, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6325267, Length=542, Percent_Identity=36.1623616236162, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI17136444, Length=615, Percent_Identity=45.5284552845528, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI17647877, Length=605, Percent_Identity=40.9917355371901, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17136446, Length=641, Percent_Identity=30.1092043681747, Blast_Score=288, Evalue=5e-78,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 68507; Mature: 68507

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01166 RNA_POL_BETA ; PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYANLYVNGKLIGSVESPDELIKYLRDNRRNGQISPFTSISYNEESSDIHISTDAGRA
CCCCEEEEECCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCC
VRPLIVVENGVSKLNEELIKKVEQGELNFSGLVKMGVIEYLDAEEEENAYIANYEKDLTK
EEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHCC
NHTHLEIDPLSILGIGAGVAPYPEHNSAPRITMAAAMGKQSLGMPLANMKLRMDTKAHLL
CCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEHHHHCHHHCCCCHHCEEEEECCHHHEE
HYPQVPIVRTKHQEILGFDRRPAGQNFVVAVMSYEGYNMEDAFIINKASIERGLARSSFF
ECCCCCEEECCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHH
RSYEGFEKRHPGGQVDRFEIPEQGVRGYRAEEAYRNLGDDGIVEVESEVKSGDVIIGKTS
HHHCCHHHCCCCCCCCCEECCHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEECCC
PPRFLEEQDISLRSKSQRRDTSITIRHGEGGIVDAVILSETKEGNRLAKIRVRDLRIPEL
CCCCCCCCCCCHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEEEEEECCCCCH
GDKFASRHGQKGVLGLIVPQEDMPFTENGVVPDLIINPHAIPSRMTIGQVLEMIGGKVGS
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCC
MNCKRIDGTIFSGDPEWELRSSMEKCGYKHNGKEVMFDGKTGKKLECEIFVGIAYYQKLH
CCEEEECCEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHH
HLVAGKMHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLIGHGTALLLKERLFDESD
HHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHHHHHCCCCC
PYECHICSNCGEVAIYDHKRGMKRCQACGETEDVQNNRKIPSVRLPYAFKLLLDELRSMG
CEEEEEECCCCCEEEEECHHHHHHHHHCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHCC
INPKLELKDRV
CCCCEECCCCC
>Mature Secondary Structure
MEKYANLYVNGKLIGSVESPDELIKYLRDNRRNGQISPFTSISYNEESSDIHISTDAGRA
CCCCEEEEECCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCC
VRPLIVVENGVSKLNEELIKKVEQGELNFSGLVKMGVIEYLDAEEEENAYIANYEKDLTK
EEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHCC
NHTHLEIDPLSILGIGAGVAPYPEHNSAPRITMAAAMGKQSLGMPLANMKLRMDTKAHLL
CCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEHHHHCHHHCCCCHHCEEEEECCHHHEE
HYPQVPIVRTKHQEILGFDRRPAGQNFVVAVMSYEGYNMEDAFIINKASIERGLARSSFF
ECCCCCEEECCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHH
RSYEGFEKRHPGGQVDRFEIPEQGVRGYRAEEAYRNLGDDGIVEVESEVKSGDVIIGKTS
HHHCCHHHCCCCCCCCCEECCHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEECCC
PPRFLEEQDISLRSKSQRRDTSITIRHGEGGIVDAVILSETKEGNRLAKIRVRDLRIPEL
CCCCCCCCCCCHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEEEEEECCCCCH
GDKFASRHGQKGVLGLIVPQEDMPFTENGVVPDLIINPHAIPSRMTIGQVLEMIGGKVGS
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCC
MNCKRIDGTIFSGDPEWELRSSMEKCGYKHNGKEVMFDGKTGKKLECEIFVGIAYYQKLH
CCEEEECCEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHH
HLVAGKMHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLIGHGTALLLKERLFDESD
HHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHHHHHCCCCC
PYECHICSNCGEVAIYDHKRGMKRCQACGETEDVQNNRKIPSVRLPYAFKLLLDELRSMG
CEEEEEECCCCCEEEEECHHHHHHHHHCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHCC
INPKLELKDRV
CCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA