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The map label for this gene is speE [H]

Identifier: 150401033

GI number: 150401033

Start: 617529

End: 618368

Strand: Reverse

Name: speE [H]

Synonym: Maeo_0602

Alternate gene names: 150401033

Gene position: 618368-617529 (Counterclockwise)

Preceding gene: 150401034

Following gene: 150401026

Centisome position: 39.4

GC content: 32.5

Gene sequence:

>840_bases
ATGAAATTTGATTTATGGTATTCAGAATATCAAACAAAAAGTCTTAAATTATCCACAAAAGTAAAGGATGTGCTTTATAC
GGGAAAATCAGAATTTCAAGAAATCCAAATTATAGATACCTATGATTTTGGAAAAGTTCTTATATTGGACAATACCTATC
AAACCACAGAAAGAGACGAATTTATATATCACGAATTAATAAGTCACCCAGCACTATTTACTCACCCAAATCCTAAAACC
GTTCTTGTTATAGGTGGTGGAGATGGCGGAACAGTTCGGGAAGTTGTAAAACACCCATCAGTAGAATCCATTGATTTTGT
GGAGCTCGATGAAAAGGTTGTTGAAGCCTGTAAAGAACACATGCCAAAATTAAGCTGTGAAATTGATAATCCAAAGGTAA
ATACATTATTCACTGATGGAATTAAATATGTAGCCGAAACAGAAAAGAAATATGATGTAATATTGGTGGATTGCCCTGAC
CCAGTTGGACCTGCAAAAGGATTATTTGAAGAAGAATTCTATAAAAATGTATTTAAATGTCTTACCGATGATGGTATAAT
GGTTCAGCAGACAGAGAGCCCATTATTTAATACAGATTTAATAAAAGATGTTGGAAATTACTTAAAAGAAGCAGGATTTG
GAATTATCAAACCATTGGTATGTACTATTCCAACATACCCCAGCGGATTTTGGAATTTTACAGTGGCATCGAAAAAATAC
AACCCATTAGAATCCGACAGGGAAGAAATAAAAGAAAAATTAAAGGAAATAGATACAATTTATTATGACGAAGATGTTCA
CATTGGTGTATTTTTAGCTACGCCAAAATATTTAAAATAA

Upstream 100 bases:

>100_bases
CTTCAATAATTGTCCCTTATCCGGCTTATTATTAAATAAAAAGACCAAGATTGACTATAATTCTAAAATTAGAGGAGTAT
CAAATTTAAGAGGGAGGATT

Downstream 100 bases:

>100_bases
GTAGATATGATAATACATTAATATATATTAAATTAATATTAAATGCCCTTAATTTTTATTTTTTTATAAGAGCTTCTTTG
AGATGCCCAGTTAATGACGA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKFDLWYSEYQTKSLKLSTKVKDVLYTGKSEFQEIQIIDTYDFGKVLILDNTYQTTERDEFIYHELISHPALFTHPNPKT
VLVIGGGDGGTVREVVKHPSVESIDFVELDEKVVEACKEHMPKLSCEIDNPKVNTLFTDGIKYVAETEKKYDVILVDCPD
PVGPAKGLFEEEFYKNVFKCLTDDGIMVQQTESPLFNTDLIKDVGNYLKEAGFGIIKPLVCTIPTYPSGFWNFTVASKKY
NPLESDREEIKEKLKEIDTIYYDEDVHIGVFLATPKYLK

Sequences:

>Translated_279_residues
MKFDLWYSEYQTKSLKLSTKVKDVLYTGKSEFQEIQIIDTYDFGKVLILDNTYQTTERDEFIYHELISHPALFTHPNPKT
VLVIGGGDGGTVREVVKHPSVESIDFVELDEKVVEACKEHMPKLSCEIDNPKVNTLFTDGIKYVAETEKKYDVILVDCPD
PVGPAKGLFEEEFYKNVFKCLTDDGIMVQQTESPLFNTDLIKDVGNYLKEAGFGIIKPLVCTIPTYPSGFWNFTVASKKY
NPLESDREEIKEKLKEIDTIYYDEDVHIGVFLATPKYLK
>Mature_279_residues
MKFDLWYSEYQTKSLKLSTKVKDVLYTGKSEFQEIQIIDTYDFGKVLILDNTYQTTERDEFIYHELISHPALFTHPNPKT
VLVIGGGDGGTVREVVKHPSVESIDFVELDEKVVEACKEHMPKLSCEIDNPKVNTLFTDGIKYVAETEKKYDVILVDCPD
PVGPAKGLFEEEFYKNVFKCLTDDGIMVQQTESPLFNTDLIKDVGNYLKEAGFGIIKPLVCTIPTYPSGFWNFTVASKKY
NPLESDREEIKEKLKEIDTIYYDEDVHIGVFLATPKYLK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=275, Percent_Identity=38.1818181818182, Blast_Score=203, Evalue=1e-52,
Organism=Escherichia coli, GI1786312, Length=267, Percent_Identity=36.3295880149813, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25154106, Length=265, Percent_Identity=36.2264150943396, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI212645616, Length=230, Percent_Identity=40.4347826086956, Blast_Score=170, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6325326, Length=258, Percent_Identity=41.4728682170543, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6323175, Length=280, Percent_Identity=36.4285714285714, Blast_Score=175, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24645443, Length=277, Percent_Identity=40.7942238267148, Blast_Score=213, Evalue=8e-56,
Organism=Drosophila melanogaster, GI21355941, Length=214, Percent_Identity=38.785046728972, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31929; Mature: 31929

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDLWYSEYQTKSLKLSTKVKDVLYTGKSEFQEIQIIDTYDFGKVLILDNTYQTTERDE
CCEEEEHHHHCCCEEEEHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHH
FIYHELISHPALFTHPNPKTVLVIGGGDGGTVREVVKHPSVESIDFVELDEKVVEACKEH
HHHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
MPKLSCEIDNPKVNTLFTDGIKYVAETEKKYDVILVDCPDPVGPAKGLFEEEFYKNVFKC
CCCEEEEECCCEEEEEEHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LTDDGIMVQQTESPLFNTDLIKDVGNYLKEAGFGIIKPLVCTIPTYPSGFWNFTVASKKY
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEECCCC
NPLESDREEIKEKLKEIDTIYYDEDVHIGVFLATPKYLK
CCCHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKFDLWYSEYQTKSLKLSTKVKDVLYTGKSEFQEIQIIDTYDFGKVLILDNTYQTTERDE
CCEEEEHHHHCCCEEEEHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHH
FIYHELISHPALFTHPNPKTVLVIGGGDGGTVREVVKHPSVESIDFVELDEKVVEACKEH
HHHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
MPKLSCEIDNPKVNTLFTDGIKYVAETEKKYDVILVDCPDPVGPAKGLFEEEFYKNVFKC
CCCEEEEECCCEEEEEEHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LTDDGIMVQQTESPLFNTDLIKDVGNYLKEAGFGIIKPLVCTIPTYPSGFWNFTVASKKY
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEECCCC
NPLESDREEIKEKLKEIDTIYYDEDVHIGVFLATPKYLK
CCCHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]