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The map label for this gene is nikA [C]

Identifier: 150400944

GI number: 150400944

Start: 533022

End: 534497

Strand: Reverse

Name: nikA [C]

Synonym: Maeo_0512

Alternate gene names: 150400944

Gene position: 534497-533022 (Counterclockwise)

Preceding gene: 150400945

Following gene: 150400943

Centisome position: 34.06

GC content: 31.71

Gene sequence:

>1476_bases
GTGAAAATAGTTTTAAGAAATGAAATTTGCGATAAATTAGAAAATATTGTAAAAAATTTAAAAATAACAAAACATTGCAT
AGGTTGTGAAGGATTGGATTTAAATAATAAAGACCCGCACCATCACCCGTCCATTGAAATAACCCAAAAATGCAACCATA
ATTGTATTTTTTGTTATTCTAAATTGGTAGGCGTAAAATCTGGAATTTATGGAATTGACCCCAACAATCCAAATACAAAG
GGACACACAGGTATAACGATAAGCCAGTATGGAGAACCCCTTACATATCCCAATAAAGTTAAAAAAGCAATAGAATTTAC
AAAAAATAATAATTTAAGATGCGATTTGCAAACCAATGGAGTATATTTAAACGAAAATCTTTTAAAAGAATTTAAAGAGT
TGGGATTAGATATAATTATGATAAGTTTAAGTGCTTCAACACCTGAAACCCATAAAATTATTGCTAATTCCGATACATAC
GAAAAGATATTAAATAATATCAAATTAAGCTCAAAATATTTCCACACCATAGTAAGAAGTGTATATATACCAGAATTTAA
CGAAAATGAATTAATTGAAATGGCAAAATATTTAAACGATAATACCGAAGTTAAGGAAATTATGATTCACCAGTTGGTAG
TCTATAATAAAAACATTGGAGAGTTAGAAAACAAAGGAAATATAAATAAAGTTGGTAAAATTAAAGACCTTTTATTATTA
GTAGATGAAATGAAAAAAAATGCACCAAATATAAATATAACCATTAAAGGCTGTTTGCTGGTTAGACAGGCAGTTGCCTA
TGCAATTGATAGAGAAGGAATATCTAAAAATCTATTCAATGGAATTCCACAACCAACAGGGCATTTACTATTGCCAGAAT
TTAAGAACGGTCCAAAAAATGCTAAACCATACTCATACAATCCAGAAAAAGCCAGACAACTTCTTGCAGAGGCAGGCTAC
AAAGACACCGATGGAGATGGCATATTGGATAAGGATGGAAAACCATTAAAACTTGCCCTTGTAACAGGAGACAGACAGAT
GGAAAGAGATACCGCAGTTTATATTCAGAAAAACTTGAAAGACTTAGGAATTGATGTGGAAATTGCAAGTTTAGAAAGCA
AGGCAAGAAAAGAAAGGGCTAAGAAAGGAGATTTCGATATTTGCAGATGTCATCCATGGGTAGCTCCACCGGTTAGATAC
TTAAAATGGAGATGCACCGACGATGGCTATGATAACTACGGATGTAAATTTGGAGTAAATGACAGAGTTAAGGAATTAAT
TAATATAATCTACACTGGAAATGATGAAGAAATTAAAAATGCATGGAATGAGCTCTGGAATATAGAATATGATTTCTGCC
CGGCTACAGGAGTTTATGTTAGACCGAGAGCATTTGTATTTAAAGACAATGTCGAAGGATTTAGATTTGATGCTGATGTG
GGATATATTGATTTAAGCAATGTTGTAATTAAATAA

Upstream 100 bases:

>100_bases
ATGAAACAGGACATATATTAAAACATTTAAAAGAATAATTTATTATGGGGAGCTCCCATTATAGAGAATTATAATATATA
AAAAATAAGTTCGTGAGACC

Downstream 100 bases:

>100_bases
AAAATAACTAAATAATCAAATAAACAAATTATCTCTTTTTATTTTTTAAGGTGAGCTCATGAAAAATTTTATATTAAGGA
GGTTATTTTTGGCAATACCA

Product: radical SAM domain-containing protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKIVLRNEICDKLENIVKNLKITKHCIGCEGLDLNNKDPHHHPSIEITQKCNHNCIFCYSKLVGVKSGIYGIDPNNPNTK
GHTGITISQYGEPLTYPNKVKKAIEFTKNNNLRCDLQTNGVYLNENLLKEFKELGLDIIMISLSASTPETHKIIANSDTY
EKILNNIKLSSKYFHTIVRSVYIPEFNENELIEMAKYLNDNTEVKEIMIHQLVVYNKNIGELENKGNINKVGKIKDLLLL
VDEMKKNAPNINITIKGCLLVRQAVAYAIDREGISKNLFNGIPQPTGHLLLPEFKNGPKNAKPYSYNPEKARQLLAEAGY
KDTDGDGILDKDGKPLKLALVTGDRQMERDTAVYIQKNLKDLGIDVEIASLESKARKERAKKGDFDICRCHPWVAPPVRY
LKWRCTDDGYDNYGCKFGVNDRVKELINIIYTGNDEEIKNAWNELWNIEYDFCPATGVYVRPRAFVFKDNVEGFRFDADV
GYIDLSNVVIK

Sequences:

>Translated_491_residues
MKIVLRNEICDKLENIVKNLKITKHCIGCEGLDLNNKDPHHHPSIEITQKCNHNCIFCYSKLVGVKSGIYGIDPNNPNTK
GHTGITISQYGEPLTYPNKVKKAIEFTKNNNLRCDLQTNGVYLNENLLKEFKELGLDIIMISLSASTPETHKIIANSDTY
EKILNNIKLSSKYFHTIVRSVYIPEFNENELIEMAKYLNDNTEVKEIMIHQLVVYNKNIGELENKGNINKVGKIKDLLLL
VDEMKKNAPNINITIKGCLLVRQAVAYAIDREGISKNLFNGIPQPTGHLLLPEFKNGPKNAKPYSYNPEKARQLLAEAGY
KDTDGDGILDKDGKPLKLALVTGDRQMERDTAVYIQKNLKDLGIDVEIASLESKARKERAKKGDFDICRCHPWVAPPVRY
LKWRCTDDGYDNYGCKFGVNDRVKELINIIYTGNDEEIKNAWNELWNIEYDFCPATGVYVRPRAFVFKDNVEGFRFDADV
GYIDLSNVVIK
>Mature_491_residues
MKIVLRNEICDKLENIVKNLKITKHCIGCEGLDLNNKDPHHHPSIEITQKCNHNCIFCYSKLVGVKSGIYGIDPNNPNTK
GHTGITISQYGEPLTYPNKVKKAIEFTKNNNLRCDLQTNGVYLNENLLKEFKELGLDIIMISLSASTPETHKIIANSDTY
EKILNNIKLSSKYFHTIVRSVYIPEFNENELIEMAKYLNDNTEVKEIMIHQLVVYNKNIGELENKGNINKVGKIKDLLLL
VDEMKKNAPNINITIKGCLLVRQAVAYAIDREGISKNLFNGIPQPTGHLLLPEFKNGPKNAKPYSYNPEKARQLLAEAGY
KDTDGDGILDKDGKPLKLALVTGDRQMERDTAVYIQKNLKDLGIDVEIASLESKARKERAKKGDFDICRCHPWVAPPVRY
LKWRCTDDGYDNYGCKFGVNDRVKELINIIYTGNDEEIKNAWNELWNIEYDFCPATGVYVRPRAFVFKDNVEGFRFDADV
GYIDLSNVVIK

Specific function: Involved In A Nickel Transport System, Probably Represents The Nickel Binder. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789887, Length=189, Percent_Identity=27.5132275132275, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 55836; Mature: 55836

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLRNEICDKLENIVKNLKITKHCIGCEGLDLNNKDPHHHPSIEITQKCNHNCIFCYS
CEEEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEH
KLVGVKSGIYGIDPNNPNTKGHTGITISQYGEPLTYPNKVKKAIEFTKNNNLRCDLQTNG
HHHHHHCCEEECCCCCCCCCCCCCEEHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCC
VYLNENLLKEFKELGLDIIMISLSASTPETHKIIANSDTYEKILNNIKLSSKYFHTIVRS
EEECHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHH
VYIPEFNENELIEMAKYLNDNTEVKEIMIHQLVVYNKNIGELENKGNINKVGKIKDLLLL
HCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
VDEMKKNAPNINITIKGCLLVRQAVAYAIDREGISKNLFNGIPQPTGHLLLPEFKNGPKN
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEECCCCCCCCCC
AKPYSYNPEKARQLLAEAGYKDTDGDGILDKDGKPLKLALVTGDRQMERDTAVYIQKNLK
CCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
DLGIDVEIASLESKARKERAKKGDFDICRCHPWVAPPVRYLKWRCTDDGYDNYGCKFGVN
HCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEEEEECCCCCCCCCCEECCC
DRVKELINIIYTGNDEEIKNAWNELWNIEYDFCPATGVYVRPRAFVFKDNVEGFRFDADV
HHHHHHHHEEECCCHHHHHHHHHHHCCCEEECCCCCCEEECCEEEEEECCCCCEEEECCC
GYIDLSNVVIK
CEEECCCEEEC
>Mature Secondary Structure
MKIVLRNEICDKLENIVKNLKITKHCIGCEGLDLNNKDPHHHPSIEITQKCNHNCIFCYS
CEEEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEH
KLVGVKSGIYGIDPNNPNTKGHTGITISQYGEPLTYPNKVKKAIEFTKNNNLRCDLQTNG
HHHHHHCCEEECCCCCCCCCCCCCEEHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCC
VYLNENLLKEFKELGLDIIMISLSASTPETHKIIANSDTYEKILNNIKLSSKYFHTIVRS
EEECHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHH
VYIPEFNENELIEMAKYLNDNTEVKEIMIHQLVVYNKNIGELENKGNINKVGKIKDLLLL
HCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
VDEMKKNAPNINITIKGCLLVRQAVAYAIDREGISKNLFNGIPQPTGHLLLPEFKNGPKN
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEECCCCCCCCCC
AKPYSYNPEKARQLLAEAGYKDTDGDGILDKDGKPLKLALVTGDRQMERDTAVYIQKNLK
CCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
DLGIDVEIASLESKARKERAKKGDFDICRCHPWVAPPVRYLKWRCTDDGYDNYGCKFGVN
HCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEEEEECCCCCCCCCCEECCC
DRVKELINIIYTGNDEEIKNAWNELWNIEYDFCPATGVYVRPRAFVFKDNVEGFRFDADV
HHHHHHHHEEECCCHHHHHHHHHHHCCCEEECCCCCCEEECCEEEEEECCCCCEEEECCC
GYIDLSNVVIK
CEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]