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The map label for this gene is thiD [C]

Identifier: 150400892

GI number: 150400892

Start: 481103

End: 482392

Strand: Direct

Name: thiD [C]

Synonym: Maeo_0460

Alternate gene names: 150400892

Gene position: 481103-482392 (Clockwise)

Preceding gene: 150400891

Following gene: 150400893

Centisome position: 30.65

GC content: 30.0

Gene sequence:

>1290_bases
TTGAATTCTATAAATGTATTATCCATAGGAGGATATGACCCAACAGGCGGAGCAGGAGTTATAGCAGATGCAAAAACCAT
CGAAATACTTGACTGTAATCCACTAACAATTACTACTACAATAATACCACAAAACAACCAGAAAGTATATTCTCAATTTA
ATTTACCAAAGGAGGAAATAGAAAACCAGTTTAAATCAATATTTTCTGATTTTGAAGTATCAACTGTGAAGACTGGTGTA
ATAAATAAAGATACCATAGATCTAATATTAAAATATCATAAAAAATATAATTTTAAAATTATTTGTGACCCAGTTTTAAA
ATCCACAACAAACCATAATTTTGTAGATAATGAGTTAATAAAAAAATATTTTTCATTATTTGAAAAATCGTGTATTATTA
CCCCAAATAAAGAGGAATACGATAAATTAAAGGAGTTTGAAGAATACAATGATTTGAAAAATAAAAACATTTACACACTG
ATTACCGGCGTAGAAGATAAATTATTATTTAATGATATTGAATTAAGAACCTACGGCGGAAAAAAAATAGACAAAGAATG
CCACGGCACAGGTTGTGTATTTTCTTCGGCAATTGCATCATTTATAGCAAAAGATTATGATATCGTAAATGCAATAAGGA
GGGCAAAAATCGTAGTTGTGGGTTCTGTAATTTATGCAAATAAAACAAAATACGGTTATAATTCAAATCCAGTTTATATA
AATAGGGAAAAAGTTATAAAAAATCTAAATTATGCATTATACCTATTGAATAAGGTTAATTTTGAGGATTTTGTTCCAGA
AGTTGGTTCAAATCTTGCAGAGAGCTCCCTATTGCCAAAACGATATAATGATGTTGCAGCCCTTACTGGAAGAATTATAA
AAAATAAATTAGGCGGAATATTTGTAGTCGGAGATATTGAATTTGGAGCATCGGAACATATTGCTAAAATAATTCTTGCT
GCAAGTAGTTTTGACCCCAAAATAAGGAGCTGTCTCAATATACGATATAGTGAAGATACAATAAACCTATTGGAAGAATA
TAGTGATTTTTCCATATCTTCGTTTAATAGGGGGGACGAACCTAAAAGCGTATCTTCTATGGAGTGGGGAACTAAATTTG
CCTGTGAAAATTATTCAAAACAGAACGATTTCTTGGGAGCTCCCGATATTATATATGATAAAGGAGGAGACGGAAAAGAG
CCTATGATAAGGGTATTGGGCAATAACTCCATAGAAGTAGTAAAAAAAGTGATAAAAATTATAAATTTAAATAAGATGCA
TTGTAAATAA

Upstream 100 bases:

>100_bases
AGCCCCTTCCGTAAGGGAGGGGTAATTCACTATACAACAATGAATAATTAATAACTAATTTATATTTATCTATAATTTAT
ATTAATTTACGGTGATTTTT

Downstream 100 bases:

>100_bases
TGTATAGAAAATTATATATAAAGAATTGTATGTTAGTTTATTTAATTTATTTAATGGCGCATATCAAGGTGATAATTTGG
CAAATATAGTAGTTAAAAAA

Product: phosphomethylpyrimidine kinase

Products: ADP; 4-amino-2-methyl-5-diphosphomethylpyrimidine

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNSINVLSIGGYDPTGGAGVIADAKTIEILDCNPLTITTTIIPQNNQKVYSQFNLPKEEIENQFKSIFSDFEVSTVKTGV
INKDTIDLILKYHKKYNFKIICDPVLKSTTNHNFVDNELIKKYFSLFEKSCIITPNKEEYDKLKEFEEYNDLKNKNIYTL
ITGVEDKLLFNDIELRTYGGKKIDKECHGTGCVFSSAIASFIAKDYDIVNAIRRAKIVVVGSVIYANKTKYGYNSNPVYI
NREKVIKNLNYALYLLNKVNFEDFVPEVGSNLAESSLLPKRYNDVAALTGRIIKNKLGGIFVVGDIEFGASEHIAKIILA
ASSFDPKIRSCLNIRYSEDTINLLEEYSDFSISSFNRGDEPKSVSSMEWGTKFACENYSKQNDFLGAPDIIYDKGGDGKE
PMIRVLGNNSIEVVKKVIKIINLNKMHCK

Sequences:

>Translated_429_residues
MNSINVLSIGGYDPTGGAGVIADAKTIEILDCNPLTITTTIIPQNNQKVYSQFNLPKEEIENQFKSIFSDFEVSTVKTGV
INKDTIDLILKYHKKYNFKIICDPVLKSTTNHNFVDNELIKKYFSLFEKSCIITPNKEEYDKLKEFEEYNDLKNKNIYTL
ITGVEDKLLFNDIELRTYGGKKIDKECHGTGCVFSSAIASFIAKDYDIVNAIRRAKIVVVGSVIYANKTKYGYNSNPVYI
NREKVIKNLNYALYLLNKVNFEDFVPEVGSNLAESSLLPKRYNDVAALTGRIIKNKLGGIFVVGDIEFGASEHIAKIILA
ASSFDPKIRSCLNIRYSEDTINLLEEYSDFSISSFNRGDEPKSVSSMEWGTKFACENYSKQNDFLGAPDIIYDKGGDGKE
PMIRVLGNNSIEVVKKVIKIINLNKMHCK
>Mature_429_residues
MNSINVLSIGGYDPTGGAGVIADAKTIEILDCNPLTITTTIIPQNNQKVYSQFNLPKEEIENQFKSIFSDFEVSTVKTGV
INKDTIDLILKYHKKYNFKIICDPVLKSTTNHNFVDNELIKKYFSLFEKSCIITPNKEEYDKLKEFEEYNDLKNKNIYTL
ITGVEDKLLFNDIELRTYGGKKIDKECHGTGCVFSSAIASFIAKDYDIVNAIRRAKIVVVGSVIYANKTKYGYNSNPVYI
NREKVIKNLNYALYLLNKVNFEDFVPEVGSNLAESSLLPKRYNDVAALTGRIIKNKLGGIFVVGDIEFGASEHIAKIILA
ASSFDPKIRSCLNIRYSEDTINLLEEYSDFSISSFNRGDEPKSVSSMEWGTKFACENYSKQNDFLGAPDIIYDKGGDGKE
PMIRVLGNNSIEVVKKVIKIINLNKMHCK

Specific function: Catalyzes The Phosphorylation Of Hmp-P To Hmp-Pp. [C]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=24.2307692307692, Blast_Score=101, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6325378, Length=251, Percent_Identity=28.6852589641434, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324517, Length=160, Percent_Identity=34.375, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324997, Length=148, Percent_Identity=35.1351351351351, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013749
- InterPro:   IPR019293 [H]

Pfam domain/function: PF10120 MethylPyrKinase; PF08543 Phos_pyr_kin [H]

EC number: 2.7.4.7

Molecular weight: Translated: 48334; Mature: 48334

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSINVLSIGGYDPTGGAGVIADAKTIEILDCNPLTITTTIIPQNNQKVYSQFNLPKEEI
CCCCEEEEECCCCCCCCCEEEECCCEEEEEECCCEEEEEEEEECCCHHHHHHCCCCHHHH
ENQFKSIFSDFEVSTVKTGVINKDTIDLILKYHKKYNFKIICDPVLKSTTNHNFVDNELI
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCCHHHHH
KKYFSLFEKSCIITPNKEEYDKLKEFEEYNDLKNKNIYTLITGVEDKLLFNDIELRTYGG
HHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEEECCC
KKIDKECHGTGCVFSSAIASFIAKDYDIVNAIRRAKIVVVGSVIYANKTKYGYNSNPVYI
CCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEEEEEECCCCCCCCCCCEEE
NREKVIKNLNYALYLLNKVNFEDFVPEVGSNLAESSLLPKRYNDVAALTGRIIKNKLGGI
EHHHHHHHCCEEEEEEECCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
FVVGDIEFGASEHIAKIILAASSFDPKIRSCLNIRYSEDTINLLEEYSDFSISSFNRGDE
EEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCEECHHHHHHHHHHCCCCCCCCCCCCC
PKSVSSMEWGTKFACENYSKQNDFLGAPDIIYDKGGDGKEPMIRVLGNNSIEVVKKVIKI
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHH
INLNKMHCK
HCCCCCCCC
>Mature Secondary Structure
MNSINVLSIGGYDPTGGAGVIADAKTIEILDCNPLTITTTIIPQNNQKVYSQFNLPKEEI
CCCCEEEEECCCCCCCCCEEEECCCEEEEEECCCEEEEEEEEECCCHHHHHHCCCCHHHH
ENQFKSIFSDFEVSTVKTGVINKDTIDLILKYHKKYNFKIICDPVLKSTTNHNFVDNELI
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCCHHHHH
KKYFSLFEKSCIITPNKEEYDKLKEFEEYNDLKNKNIYTLITGVEDKLLFNDIELRTYGG
HHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEEECCC
KKIDKECHGTGCVFSSAIASFIAKDYDIVNAIRRAKIVVVGSVIYANKTKYGYNSNPVYI
CCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEEEEEECCCCCCCCCCCEEE
NREKVIKNLNYALYLLNKVNFEDFVPEVGSNLAESSLLPKRYNDVAALTGRIIKNKLGGI
EHHHHHHHCCEEEEEEECCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
FVVGDIEFGASEHIAKIILAASSFDPKIRSCLNIRYSEDTINLLEEYSDFSISSFNRGDE
EEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCEECHHHHHHHHHHCCCCCCCCCCCCC
PKSVSSMEWGTKFACENYSKQNDFLGAPDIIYDKGGDGKEPMIRVLGNNSIEVVKKVIKI
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHH
INLNKMHCK
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 4-amino-2-methyl-5-phosphomethylpyrimidine

Specific reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]