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The map label for this gene is lacA [C]

Identifier: 150400850

GI number: 150400850

Start: 437749

End: 438420

Strand: Direct

Name: lacA [C]

Synonym: Maeo_0418

Alternate gene names: 150400850

Gene position: 437749-438420 (Clockwise)

Preceding gene: 150400849

Following gene: 150400851

Centisome position: 27.89

GC content: 28.27

Gene sequence:

>672_bases
ATGAAAAATTCTGTATATTCCCATCAACTTGTTATATATAAGATCATAAAAAATTTATGTACAAATAAAATATTATATGG
CATATTTAGAATTTTAGAAGAAGCCAGATGGGAATACGAATATAAAAGATATCGGGAAAAATACGATATTCATCCAACAT
TTAGATTCAATGGTCCAGGAATTACTCTTTACGGCAATGGTAAAATAATATTGGGGGAAAATTCATATATTGGTAGATAC
AGCTCTATTCAATCGGTAGATGGATGTATTGTGAAAATTGGTAATAATTGTGCAATTAGTCATTATGTTATGATTTATAC
TGCAAATGCTGTTGCTGATCAAGATTTTAATAAGCCCAGAGACCAAAGAAAAATTAAAAAGGGTAATGTAATAATAGAAG
ATTTTTGTTGGATTGGTGCTCAAACATTTATTACTGAAGGAGTAAATATAGGTCAAAATGCTGCTGTTGTTGGAGCGAAT
TCTGTTGTTACAAAAGATATTCCCCCACATTCAATTGCAGTAGGATGTCCCACAAAAGTTATAAAATTTAAATCTTATTT
AAATGATAAAGACATAATAAAATTAGCAAAAGATTACTGGAACATTTTACATCAAAATCTTAAAAAACATTTACTTACCC
AATATGAAGAACTAAATAATTTAGGGGATTAA

Upstream 100 bases:

>100_bases
CAATATCCTACATAATGCCACCAGAAAGAAGTGTTGATATTGATAATGAATTTGATTTTCTATTGGCTGAATTTATCTTA
AAAAAATAAAAAGTGATACA

Downstream 100 bases:

>100_bases
AATGTGAGACATTCAAAAGGAATTAATTAAAAAGATTCTAAAATATAAAATAACGAGAAAACTAGTAATTAAATATGGCC
ACACAATAACATGGTTAAAA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKNSVYSHQLVIYKIIKNLCTNKILYGIFRILEEARWEYEYKRYREKYDIHPTFRFNGPGITLYGNGKIILGENSYIGRY
SSIQSVDGCIVKIGNNCAISHYVMIYTANAVADQDFNKPRDQRKIKKGNVIIEDFCWIGAQTFITEGVNIGQNAAVVGAN
SVVTKDIPPHSIAVGCPTKVIKFKSYLNDKDIIKLAKDYWNILHQNLKKHLLTQYEELNNLGD

Sequences:

>Translated_223_residues
MKNSVYSHQLVIYKIIKNLCTNKILYGIFRILEEARWEYEYKRYREKYDIHPTFRFNGPGITLYGNGKIILGENSYIGRY
SSIQSVDGCIVKIGNNCAISHYVMIYTANAVADQDFNKPRDQRKIKKGNVIIEDFCWIGAQTFITEGVNIGQNAAVVGAN
SVVTKDIPPHSIAVGCPTKVIKFKSYLNDKDIIKLAKDYWNILHQNLKKHLLTQYEELNNLGD
>Mature_223_residues
MKNSVYSHQLVIYKIIKNLCTNKILYGIFRILEEARWEYEYKRYREKYDIHPTFRFNGPGITLYGNGKIILGENSYIGRY
SSIQSVDGCIVKIGNNCAISHYVMIYTANAVADQDFNKPRDQRKIKKGNVIIEDFCWIGAQTFITEGVNIGQNAAVVGAN
SVVTKDIPPHSIAVGCPTKVIKFKSYLNDKDIIKLAKDYWNILHQNLKKHLLTQYEELNNLGD

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=132, Percent_Identity=35.6060606060606, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1786664, Length=142, Percent_Identity=34.5070422535211, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788345, Length=134, Percent_Identity=32.8358208955224, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 25546; Mature: 25546

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSVYSHQLVIYKIIKNLCTNKILYGIFRILEEARWEYEYKRYREKYDIHPTFRFNGPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCE
ITLYGNGKIILGENSYIGRYSSIQSVDGCIVKIGNNCAISHYVMIYTANAVADQDFNKPR
EEEEECCEEEECCCCCCCCHHHHHCCCCEEEEECCCCEEEEEEEEEEECHHCCCCCCCCH
DQRKIKKGNVIIEDFCWIGAQTFITEGVNIGQNAAVVGANSVVTKDIPPHSIAVGCPTKV
HHHHHHCCCEEEEHHHHHCHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCEEECCCHHH
IKFKSYLNDKDIIKLAKDYWNILHQNLKKHLLTQYEELNNLGD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNSVYSHQLVIYKIIKNLCTNKILYGIFRILEEARWEYEYKRYREKYDIHPTFRFNGPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCE
ITLYGNGKIILGENSYIGRYSSIQSVDGCIVKIGNNCAISHYVMIYTANAVADQDFNKPR
EEEEECCEEEECCCCCCCCHHHHHCCCCEEEEECCCCEEEEEEEEEEECHHCCCCCCCCH
DQRKIKKGNVIIEDFCWIGAQTFITEGVNIGQNAAVVGANSVVTKDIPPHSIAVGCPTKV
HHHHHHCCCEEEEHHHHHCHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCEEECCCHHH
IKFKSYLNDKDIIKLAKDYWNILHQNLKKHLLTQYEELNNLGD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]