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The map label for this gene is gmhB [H]

Identifier: 150400831

GI number: 150400831

Start: 414497

End: 415009

Strand: Reverse

Name: gmhB [H]

Synonym: Maeo_0399

Alternate gene names: 150400831

Gene position: 415009-414497 (Counterclockwise)

Preceding gene: 150400832

Following gene: 150400830

Centisome position: 26.44

GC content: 27.49

Gene sequence:

>513_bases
ATGAATAATCCAGCAAATAAAGCCATATTTCTTGATAGGGATGGAGTCATAAATAAAAAACTTGAAGGAGATTATGTTAA
ATCCACTGATGAGTTTGAATTGCTACCAAAAGTAAAAGAAGCCTTAATAGAATTTAAAAATAAAGGTTATTTATTAATTG
TAATAACAAATCAGCAAGGAATTGGAAAAGGTTTAATGAGCGAAAATGATTTAAAAATTGTGCATAATTATATGACTAAA
TTATTGCCTGAAATTGATGATATATACTATTGTCCTCATTTAGATGGAACTTGCAATTGTAGAAAACCTAAAAATGAAAT
GCTTTTAAAGGCAAAGGATAAGTGGAATATTGATTTTAATAAAAGTTGGATGATAGGAGATAGTGAAAGCGATATAATTT
GTGGGAATAGTGTGGGATGCAAAACTGTAAAAATAATACGAAATGACAGGGAACGGAAGACTAATTCAAATTATACTTCC
AAACAATTGTTTGATTGTATTAATATATTATAA

Upstream 100 bases:

>100_bases
GGGCATGATTTAATAGATGCCGTTAAATTGGCAAATAAGGCGAGCTCAATAGTAGTTGGAAAAATAGGAACTGCAACAGT
TAATTTGGAGGAGTTATTTA

Downstream 100 bases:

>100_bases
AATTATCAATAAATCTCGTGCTGGTTTTGATTTCGTCTTTATAGAAAAAAAATAGATATGATGAACTTTAAATGGCTGCC
TTTTTCATTAACTATAATAT

Product: histidinol-phosphate phosphatase family protein

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-; $alanine

Alternate protein names: D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MNNPANKAIFLDRDGVINKKLEGDYVKSTDEFELLPKVKEALIEFKNKGYLLIVITNQQGIGKGLMSENDLKIVHNYMTK
LLPEIDDIYYCPHLDGTCNCRKPKNEMLLKAKDKWNIDFNKSWMIGDSESDIICGNSVGCKTVKIIRNDRERKTNSNYTS
KQLFDCINIL

Sequences:

>Translated_170_residues
MNNPANKAIFLDRDGVINKKLEGDYVKSTDEFELLPKVKEALIEFKNKGYLLIVITNQQGIGKGLMSENDLKIVHNYMTK
LLPEIDDIYYCPHLDGTCNCRKPKNEMLLKAKDKWNIDFNKSWMIGDSESDIICGNSVGCKTVKIIRNDRERKTNSNYTS
KQLFDCINIL
>Mature_170_residues
MNNPANKAIFLDRDGVINKKLEGDYVKSTDEFELLPKVKEALIEFKNKGYLLIVITNQQGIGKGLMSENDLKIVHNYMTK
LLPEIDDIYYCPHLDGTCNCRKPKNEMLLKAKDKWNIDFNKSWMIGDSESDIICGNSVGCKTVKIIRNDRERKTNSNYTS
KQLFDCINIL

Specific function: Converts the D-glycero-beta-D-manno-heptose 1,7- bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1- phosphate by removing the phosphate group at the C-7 position [H]

COG id: COG0241

COG function: function code E; Histidinol phosphatase and related phosphatases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gmhB family [H]

Homologues:

Organism=Escherichia coli, GI1786399, Length=155, Percent_Identity=36.7741935483871, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082027, Length=153, Percent_Identity=31.3725490196078, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR004446
- InterPro:   IPR006543
- InterPro:   IPR013954 [H]

Pfam domain/function: PF08645 PNK3P [H]

EC number: 6.3.2.10

Molecular weight: Translated: 19527; Mature: 19527

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPANKAIFLDRDGVINKKLEGDYVKSTDEFELLPKVKEALIEFKNKGYLLIVITNQQG
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
IGKGLMSENDLKIVHNYMTKLLPEIDDIYYCPHLDGTCNCRKPKNEMLLKAKDKWNIDFN
CCCCCCCCCCHHHHHHHHHHHCCCHHCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCC
KSWMIGDSESDIICGNSVGCKTVKIIRNDRERKTNSNYTSKQLFDCINIL
CEEEECCCCCCEEECCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNNPANKAIFLDRDGVINKKLEGDYVKSTDEFELLPKVKEALIEFKNKGYLLIVITNQQG
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
IGKGLMSENDLKIVHNYMTKLLPEIDDIYYCPHLDGTCNCRKPKNEMLLKAKDKWNIDFN
CCCCCCCCCCHHHHHHHHHHHCCCHHCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCC
KSWMIGDSESDIICGNSVGCKTVKIIRNDRERKTNSNYTSKQLFDCINIL
CEEEECCCCCCEEECCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine; D-alanyl-D-alanine

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L- $alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12101286 [H]