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The map label for this gene is hldD [H]

Identifier: 150400830

GI number: 150400830

Start: 413407

End: 414324

Strand: Reverse

Name: hldD [H]

Synonym: Maeo_0398

Alternate gene names: 150400830

Gene position: 414324-413407 (Counterclockwise)

Preceding gene: 150400831

Following gene: 150400829

Centisome position: 26.4

GC content: 33.33

Gene sequence:

>918_bases
ATGAATGTTATGATAACAGGCGGTGCAGGATTTATCGGTTCCAATATTGCATTGGAATTGCAGGACAAAGGTTATGATAC
AGTTGTTTTAGATGATTTTTCAAGTGGGAATTTTAAAAATCTCCTGGGTTATGAAGGTGATGTTGTTTCTGATAGTATTT
TAGATGTTGATTTAGATAGATTTAAAGATATTGATGCTATTTTTCATGAGGCCGCTATAACAGATACAACAGTCAGCAAC
CAAAAATTAATGATGCAAATAAATACAGAGGGGTTCAGAAGACTTTTAGATTTTTCTGTTGAAAATGATATAAAGTTCAT
TTATGCATCCTCTGCGGCAACTTATGGTGATGCTGAATCTCCTCAAAAGGAGGAATATGCTGGAAGACCAAATAATATAT
ATGGTTTTTCAAAATGGATATGCGACTGCATGGCTAAAAAATATATGGAGAAGTATCCTGACAGCCATATAGTTGGTTTA
AGATATTTTAATGTTTTTGGACCAAGGGAGCAGTATAAGGGAAAAATGGCTTCAATGGTTTGGCAGTTGGCGAAACAAAT
GGTTGAGGGAAAAAATCCAAGAATATTCAAATGGGGGGAGCAGAAGAGAGACCAAGTTTATGTTAAAAATATTGTTCAAA
TAAATTTATTGGCATTGGATGCTAAGGAGAGCTGTATTGTAAATGCAGGTAGTGGAAATGCCGTTTCCTTTAATCATATT
ATCGAAGTTTTAAATGATGTTTTGGGCTTTGATTACGAACCTGAATATATTGACAACCCTTATGAAGAGTTTTATCAAGA
TTTTACTCAGGCGGATTTAACCAATGCTAAAAAATATTTAGATTATGAACCCAAATGGAATTTTGAAGATGGGGTTAAAG
ATTATGTTGAATGGTTAAAGGAAAATAGGTATATTTAA

Upstream 100 bases:

>100_bases
TGGCTGCCTTTTTCATTAACTATAATATAAGGGATAAACTTTAAATATTATATAAAACACATTGTAATTTAGTTTAATTT
TTTTAGCATAGGGTGATTAA

Downstream 100 bases:

>100_bases
AAATATTATTGGGTGATATGTATGAAAAAGGCAATTATTACGGGAATAACCGGACAGGATGGATACTATTTAACAAAATT
ACTTTTGGAAAAAGGATATG

Product: ADP-L-glycero-D-manno-heptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNVMITGGAGFIGSNIALELQDKGYDTVVLDDFSSGNFKNLLGYEGDVVSDSILDVDLDRFKDIDAIFHEAAITDTTVSN
QKLMMQINTEGFRRLLDFSVENDIKFIYASSAATYGDAESPQKEEYAGRPNNIYGFSKWICDCMAKKYMEKYPDSHIVGL
RYFNVFGPREQYKGKMASMVWQLAKQMVEGKNPRIFKWGEQKRDQVYVKNIVQINLLALDAKESCIVNAGSGNAVSFNHI
IEVLNDVLGFDYEPEYIDNPYEEFYQDFTQADLTNAKKYLDYEPKWNFEDGVKDYVEWLKENRYI

Sequences:

>Translated_305_residues
MNVMITGGAGFIGSNIALELQDKGYDTVVLDDFSSGNFKNLLGYEGDVVSDSILDVDLDRFKDIDAIFHEAAITDTTVSN
QKLMMQINTEGFRRLLDFSVENDIKFIYASSAATYGDAESPQKEEYAGRPNNIYGFSKWICDCMAKKYMEKYPDSHIVGL
RYFNVFGPREQYKGKMASMVWQLAKQMVEGKNPRIFKWGEQKRDQVYVKNIVQINLLALDAKESCIVNAGSGNAVSFNHI
IEVLNDVLGFDYEPEYIDNPYEEFYQDFTQADLTNAKKYLDYEPKWNFEDGVKDYVEWLKENRYI
>Mature_305_residues
MNVMITGGAGFIGSNIALELQDKGYDTVVLDDFSSGNFKNLLGYEGDVVSDSILDVDLDRFKDIDAIFHEAAITDTTVSN
QKLMMQINTEGFRRLLDFSVENDIKFIYASSAATYGDAESPQKEEYAGRPNNIYGFSKWICDCMAKKYMEKYPDSHIVGL
RYFNVFGPREQYKGKMASMVWQLAKQMVEGKNPRIFKWGEQKRDQVYVKNIVQINLLALDAKESCIVNAGSGNAVSFNHI
IEVLNDVLGFDYEPEYIDNPYEEFYQDFTQADLTNAKKYLDYEPKWNFEDGVKDYVEWLKENRYI

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=316, Percent_Identity=24.6835443037975, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=25.531914893617, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1790049, Length=316, Percent_Identity=39.2405063291139, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1786974, Length=183, Percent_Identity=31.1475409836066, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=23.6994219653179, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=308, Percent_Identity=25.3246753246753, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=344, Percent_Identity=25.5813953488372, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=25.1572327044025, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 35039; Mature: 35039

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVMITGGAGFIGSNIALELQDKGYDTVVLDDFSSGNFKNLLGYEGDVVSDSILDVDLDR
CEEEEECCCCEECCCEEEEEECCCCCEEEEECCCCCCCHHHCCCCCCCCCCHHHCCCHHH
FKDIDAIFHEAAITDTTVSNQKLMMQINTEGFRRLLDFSVENDIKFIYASSAATYGDAES
HHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
PQKEEYAGRPNNIYGFSKWICDCMAKKYMEKYPDSHIVGLRYFNVFGPREQYKGKMASMV
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHH
WQLAKQMVEGKNPRIFKWGEQKRDQVYVKNIVQINLLALDAKESCIVNAGSGNAVSFNHI
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHEEEEEEEEECCCCCEEEECCCCCEEEHHHH
IEVLNDVLGFDYEPEYIDNPYEEFYQDFTQADLTNAKKYLDYEPKWNFEDGVKDYVEWLK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ENRYI
HCCCC
>Mature Secondary Structure
MNVMITGGAGFIGSNIALELQDKGYDTVVLDDFSSGNFKNLLGYEGDVVSDSILDVDLDR
CEEEEECCCCEECCCEEEEEECCCCCEEEEECCCCCCCHHHCCCCCCCCCCHHHCCCHHH
FKDIDAIFHEAAITDTTVSNQKLMMQINTEGFRRLLDFSVENDIKFIYASSAATYGDAES
HHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
PQKEEYAGRPNNIYGFSKWICDCMAKKYMEKYPDSHIVGLRYFNVFGPREQYKGKMASMV
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHH
WQLAKQMVEGKNPRIFKWGEQKRDQVYVKNIVQINLLALDAKESCIVNAGSGNAVSFNHI
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHEEEEEEEEECCCCCEEEECCCCCEEEHHHH
IEVLNDVLGFDYEPEYIDNPYEEFYQDFTQADLTNAKKYLDYEPKWNFEDGVKDYVEWLK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ENRYI
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]