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The map label for this gene is hycE [C]

Identifier: 150400804

GI number: 150400804

Start: 384505

End: 385641

Strand: Direct

Name: hycE [C]

Synonym: Maeo_0371

Alternate gene names: 150400804

Gene position: 384505-385641 (Clockwise)

Preceding gene: 150400803

Following gene: 150400805

Centisome position: 24.5

GC content: 35.18

Gene sequence:

>1137_bases
ATGAATGTAGTGCCAGTAGGACCGATACACCCGGTGCTAAAAGAACCCATAAGATTAAAGTTAATAGTTGAAGGAGAAAA
AGTAATTGGTTCTGAGCTTGAAATGGGGTATGTTCATAGGGGAATTGAAAGGATAATGGAGGGAAAACATCACCTAAAAG
GTATTCACCTTGCTGAAAGGGTGTGTGGTATTTGTTCATATATTCATACATACACCTTTGCAGAGTGCATAGAAAATTAT
TCAAAAATAGAAATCCCCGATAAAGCAAAATATTTAAGATTAATCACTTGCGAATTGGAACGGATACACAGTCATTTAAT
TGCATCCGCAGTTTATAATATTTCTATCGAACATGAAACTCTTGCTATGTGGGGATTAAGTGCAAGGGAGCATGTTATGG
ATTTATTAGAGATGATAACTGGGAACAGAGTAAATATGGGCTATAATTTAGTAGGTGGTGTTAGGGCCGACATAAATAAA
GAAATGATTGATAAAATCCATAAAAAATTAAATGAATTAAGGGAAGACCTTAAAAACCTCGTATCAGCATTTGAAACTGG
TCCATTGATTGGATTAAGGGGCAAAGGAATTGGGGTTTTAAAATATGCAGACATTATGAAAACTCGTGCAGTTGGTCCAG
TGGCAAGAGCTTCGGCACTTCCAGAATGTGATTGGAGATTAAGACATCCAGTATATAAAGAACTCAAATTTAAGCCGGTT
TGGTTTGAAGATGGTGACAATCATGCTCGTATGGCAGTTAGACATCATGAAGTATTGTCAAGTATTGATTTAATAGAGCA
AGCTATTGAACTATATAATGAATGTTCTGGAAGAGTTAGGGTAAAACCAGAAATTAAAGGGGGAGAAGGAGAGTGGAGAA
ATGAGGCCCATAGGGGAGAGGTATGTTATAAAATAGCCACTACAAATAATGGATTAATTAAAAGAATAATGATAAGAACC
CCCACTGTGATGAATCTTGAAGCCATGAAAATAATGTTTAAAACTTGCCCAACTATCTCTGATGCTGTAACAACATACAC
ATCAATAGACCCCTGTATATCATGCACAGAACGAACCATTGTATTAGAAGATAAAAAAACTGGAAAAATTAATAATTATT
CATTTAATATGCTTTAA

Upstream 100 bases:

>100_bases
GGAATAGCTCTTAAAAATGCTAACAAAGTAGCAAATGTAGAGTAGCATATAAAATGGTATTGATAAAATATATTAAGAAA
AATTAAAAAACTGTGATAAT

Downstream 100 bases:

>100_bases
TTAAAAACTAAAAAAGAGTTGAGATATTATGTCCTCCTCATTGTGGTATCTTTACGAGTTTGTAAGAAAAAAATGGATAT
CTAACTTCACCTGTGCTAAA

Product: NADH-ubiquinone oxidoreductase chain 49kDa

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNVVPVGPIHPVLKEPIRLKLIVEGEKVIGSELEMGYVHRGIERIMEGKHHLKGIHLAERVCGICSYIHTYTFAECIENY
SKIEIPDKAKYLRLITCELERIHSHLIASAVYNISIEHETLAMWGLSAREHVMDLLEMITGNRVNMGYNLVGGVRADINK
EMIDKIHKKLNELREDLKNLVSAFETGPLIGLRGKGIGVLKYADIMKTRAVGPVARASALPECDWRLRHPVYKELKFKPV
WFEDGDNHARMAVRHHEVLSSIDLIEQAIELYNECSGRVRVKPEIKGGEGEWRNEAHRGEVCYKIATTNNGLIKRIMIRT
PTVMNLEAMKIMFKTCPTISDAVTTYTSIDPCISCTERTIVLEDKKTGKINNYSFNML

Sequences:

>Translated_378_residues
MNVVPVGPIHPVLKEPIRLKLIVEGEKVIGSELEMGYVHRGIERIMEGKHHLKGIHLAERVCGICSYIHTYTFAECIENY
SKIEIPDKAKYLRLITCELERIHSHLIASAVYNISIEHETLAMWGLSAREHVMDLLEMITGNRVNMGYNLVGGVRADINK
EMIDKIHKKLNELREDLKNLVSAFETGPLIGLRGKGIGVLKYADIMKTRAVGPVARASALPECDWRLRHPVYKELKFKPV
WFEDGDNHARMAVRHHEVLSSIDLIEQAIELYNECSGRVRVKPEIKGGEGEWRNEAHRGEVCYKIATTNNGLIKRIMIRT
PTVMNLEAMKIMFKTCPTISDAVTTYTSIDPCISCTERTIVLEDKKTGKINNYSFNML
>Mature_378_residues
MNVVPVGPIHPVLKEPIRLKLIVEGEKVIGSELEMGYVHRGIERIMEGKHHLKGIHLAERVCGICSYIHTYTFAECIENY
SKIEIPDKAKYLRLITCELERIHSHLIASAVYNISIEHETLAMWGLSAREHVMDLLEMITGNRVNMGYNLVGGVRADINK
EMIDKIHKKLNELREDLKNLVSAFETGPLIGLRGKGIGVLKYADIMKTRAVGPVARASALPECDWRLRHPVYKELKFKPV
WFEDGDNHARMAVRHHEVLSSIDLIEQAIELYNECSGRVRVKPEIKGGEGEWRNEAHRGEVCYKIATTNNGLIKRIMIRT
PTVMNLEAMKIMFKTCPTISDAVTTYTSIDPCISCTERTIVLEDKKTGKINNYSFNML

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=268, Percent_Identity=23.8805970149254, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI260898743, Length=268, Percent_Identity=23.8805970149254, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789076, Length=379, Percent_Identity=32.4538258575198, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1788832, Length=367, Percent_Identity=31.0626702997275, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI145693162, Length=387, Percent_Identity=22.7390180878553, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17568379, Length=269, Percent_Identity=24.907063197026, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17555284, Length=269, Percent_Identity=24.5353159851301, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI221459469, Length=270, Percent_Identity=26.2962962962963, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24638644, Length=265, Percent_Identity=25.2830188679245, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.6.5.3

Molecular weight: Translated: 42930; Mature: 42930

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVPVGPIHPVLKEPIRLKLIVEGEKVIGSELEMGYVHRGIERIMEGKHHLKGIHLAER
CCCCCCCCCCHHHCCCEEEEEEEECCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VCGICSYIHTYTFAECIENYSKIEIPDKAKYLRLITCELERIHSHLIASAVYNISIEHET
HHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHH
LAMWGLSAREHVMDLLEMITGNRVNMGYNLVGGVRADINKEMIDKIHKKLNELREDLKNL
HHHHCCCHHHHHHHHHHHHCCCCEECCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHH
VSAFETGPLIGLRGKGIGVLKYADIMKTRAVGPVARASALPECDWRLRHPVYKELKFKPV
HHHHHCCCEEEECCCCCEEHHHHHHHHHHCCCCHHHHHCCCCCCCHHCCCHHHHCCCCEE
WFEDGDNHARMAVRHHEVLSSIDLIEQAIELYNECSGRVRVKPEIKGGEGEWRNEAHRGE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
VCYKIATTNNGLIKRIMIRTPTVMNLEAMKIMFKTCPTISDAVTTYTSIDPCISCTERTI
EEEEEEECCCCHHHHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCEE
VLEDKKTGKINNYSFNML
EEECCCCCCCCCEEECCC
>Mature Secondary Structure
MNVVPVGPIHPVLKEPIRLKLIVEGEKVIGSELEMGYVHRGIERIMEGKHHLKGIHLAER
CCCCCCCCCCHHHCCCEEEEEEEECCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VCGICSYIHTYTFAECIENYSKIEIPDKAKYLRLITCELERIHSHLIASAVYNISIEHET
HHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHH
LAMWGLSAREHVMDLLEMITGNRVNMGYNLVGGVRADINKEMIDKIHKKLNELREDLKNL
HHHHCCCHHHHHHHHHHHHCCCCEECCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHH
VSAFETGPLIGLRGKGIGVLKYADIMKTRAVGPVARASALPECDWRLRHPVYKELKFKPV
HHHHHCCCEEEECCCCCEEHHHHHHHHHHCCCCHHHHHCCCCCCCHHCCCHHHHCCCCEE
WFEDGDNHARMAVRHHEVLSSIDLIEQAIELYNECSGRVRVKPEIKGGEGEWRNEAHRGE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
VCYKIATTNNGLIKRIMIRTPTVMNLEAMKIMFKTCPTISDAVTTYTSIDPCISCTERTI
EEEEEEECCCCHHHHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCEE
VLEDKKTGKINNYSFNML
EEECCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]