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The map label for this gene is hycG [C]

Identifier: 150400803

GI number: 150400803

Start: 383988

End: 384449

Strand: Direct

Name: hycG [C]

Synonym: Maeo_0370

Alternate gene names: 150400803

Gene position: 383988-384449 (Clockwise)

Preceding gene: 150400802

Following gene: 150400804

Centisome position: 24.47

GC content: 38.74

Gene sequence:

>462_bases
ATGATAAAAGAGTTTTTTAGAAAACGGTCAATAAACCTCTGTATAGTAAATACTGGTGGTTGCAATGGTTGCGATATAGA
ACTTGTATCCACACTTGCACCTCGGTATGATATAGAACAATACGGTGTTTATGTCCATAACAATCCACGGGAGGCCGATA
TATTAGTAATCACTGGACCAGTTACTCTCCCATGGAAAGATAAACTTGTAGAAATATATGAAAAAACACCAGAACCAAAA
ATTGTGGTGGCAGTTGGTGCCTGTGCATTAAGTGGCGGGATATTTAAAGAGGGCCATATATATGGTCCAGTTGATAAATT
TATTCCTGTTGATGCTAAAATTGTAGGTTGCCCCCCAAGACCTTCTGAAATTATTGAAGCAGTGCTAAAAGTGGCACCCG
ATGCCCTTGCAGCAAAAGGAATAGCTCTTAAAAATGCTAACAAAGTAGCAAATGTAGAGTAG

Upstream 100 bases:

>100_bases
GATACCATTATGAAAAAAATACCCTATAAAACAGCACTAGAAAATGGTAGAAAAATGGTTGTAGAACTGTTGAAATAACA
CATATATATGTGGTGTAATT

Downstream 100 bases:

>100_bases
CATATAAAATGGTATTGATAAAATATATTAAGAAAAATTAAAAAACTGTGATAATATGAATGTAGTGCCAGTAGGACCGA
TACACCCGGTGCTAAAAGAA

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MIKEFFRKRSINLCIVNTGGCNGCDIELVSTLAPRYDIEQYGVYVHNNPREADILVITGPVTLPWKDKLVEIYEKTPEPK
IVVAVGACALSGGIFKEGHIYGPVDKFIPVDAKIVGCPPRPSEIIEAVLKVAPDALAAKGIALKNANKVANVE

Sequences:

>Translated_153_residues
MIKEFFRKRSINLCIVNTGGCNGCDIELVSTLAPRYDIEQYGVYVHNNPREADILVITGPVTLPWKDKLVEIYEKTPEPK
IVVAVGACALSGGIFKEGHIYGPVDKFIPVDAKIVGCPPRPSEIIEAVLKVAPDALAAKGIALKNANKVANVE
>Mature_153_residues
MIKEFFRKRSINLCIVNTGGCNGCDIELVSTLAPRYDIEQYGVYVHNNPREADILVITGPVTLPWKDKLVEIYEKTPEPK
IVVAVGACALSGGIFKEGHIYGPVDKFIPVDAKIVGCPPRPSEIIEAVLKVAPDALAAKGIALKNANKVANVE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=115, Percent_Identity=33.0434782608696, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789074, Length=119, Percent_Identity=44.5378151260504, Blast_Score=113, Evalue=5e-27,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=40.495867768595, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1788624, Length=111, Percent_Identity=36.9369369369369, Blast_Score=86, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=115, Percent_Identity=36.5217391304348, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859983, Length=115, Percent_Identity=34.7826086956522, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24642371, Length=115, Percent_Identity=34.7826086956522, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24651058, Length=115, Percent_Identity=33.9130434782609, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 16571; Mature: 16571

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEFFRKRSINLCIVNTGGCNGCDIELVSTLAPRYDIEQYGVYVHNNPREADILVITGP
CCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHCCEEEECCCCCCEEEEEECC
VTLPWKDKLVEIYEKTPEPKIVVAVGACALSGGIFKEGHIYGPVDKFIPVDAKIVGCPPR
EECCCHHHHHHHHHCCCCCEEEEEEECEECCCCCCCCCCEECCHHHCCCCCEEEEECCCC
PSEIIEAVLKVAPDALAAKGIALKNANKVANVE
HHHHHHHHHHHCCHHHHHCCEEEECCCCCCCCC
>Mature Secondary Structure
MIKEFFRKRSINLCIVNTGGCNGCDIELVSTLAPRYDIEQYGVYVHNNPREADILVITGP
CCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHCCEEEECCCCCCEEEEEECC
VTLPWKDKLVEIYEKTPEPKIVVAVGACALSGGIFKEGHIYGPVDKFIPVDAKIVGCPPR
EECCCHHHHHHHHHCCCCCEEEEEEECEECCCCCCCCCCEECCHHHCCCCCEEEEECCCC
PSEIIEAVLKVAPDALAAKGIALKNANKVANVE
HHHHHHHHHHHCCHHHHHCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]