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The map label for this gene is yfcB [C]

Identifier: 150400710

GI number: 150400710

Start: 300231

End: 301298

Strand: Reverse

Name: yfcB [C]

Synonym: Maeo_0275

Alternate gene names: 150400710

Gene position: 301298-300231 (Counterclockwise)

Preceding gene: 150400711

Following gene: 150400705

Centisome position: 19.2

GC content: 27.43

Gene sequence:

>1068_bases
ATGACAATAACAGAAGTAGAAACAACAATAACAGCAAAAAAAGTGCTTTGCTGCAAAGTTCCCACAAATAAAGGAGAACG
AATTAGAAAGATATTGTTGGACAACAACGAACTTAATAAAAATTATAAATTAAAAAAAGAAGATAATTATTTATTTATTC
CAATAATTTCCACAGACGAAACTGAAATTGATAAATCTATTATGGAAAATTTAAAAAAAATATACAATAATATAGAATTT
ATTGAAATTAATATGTTGGGAAATCCAAAAACTAATAAACTCAATTTTAGGGAATATTTACTTAAAAACTTTAAGAAAGA
AATAGAAAATGGGGCCGTAGTATTATCTTATGATATTATTGGAGATATTGTAATTATACAGATATCTGATAATATTGACG
AAAACATTGCAAAAAAAATAGGGGCTAAGGCCTTAGAGTTAATACCATCTGTAAAATCCGTATATCGAAGAAAAAGCGAA
ATATTGGGAGAATTTCGAGTTAGAGAATTGGAGCTCCTTGCAGGAGAATATAAAACTTTAACGATGTATAAAGAAAATGG
ATATAGATTGTGGGTAGATGTGGAAAAAGTTTATTTCTCTCCGAGATTAGGCTGGGAAAGGAAGAGAATAATGGAAAAAG
TAAAACCAGAGGATATTGTTGTGGATATGTTTTGCGGAGTTGGTCCTTTCTCAATAGCTTGTAAAAATGCAAAAAAGATA
TATGCCATAGATATAAATCCAGACGCCGTGGAGCTCCTTAAAAAGAATATCAAATTAAATAAACTCCAACATAAAATAAT
ACCCATAAATAATGATATAAGAAAAACAGAAGTTAAAGGTAATAGAATAATAATGAATCTTCCTAAATTTGCCCATTTAT
TTGTAGATACGGCACTGAAAATGGTTGAAAAAAATGGCATAATTCATTATTATACTGTTGGAACAGATTATAATGAAGCC
ATAAATTTATTTAAATCAAAATGTAATTGTGAAATAGTTGAAAAAAGAATTGTAAAATCATATTCTCCAAGAGAGCATAT
TTTTGTGTTGGATATAAAAATATATTAA

Upstream 100 bases:

>100_bases
TAAATCTTAAAAAAGAAAATAAAACACCAAAAGAAATAGCAAAAATAACAGGAATAAATTTAAAAACTGTTGAATACCAC
TACTATAAAAAAGTGAGATA

Downstream 100 bases:

>100_bases
ATAAATAAGCATTATTATATATTATTTTTCTAATTCGGACAAATCTAAGTCATCCAAATTAGTATTTTTTTCTATTTCCT
TTTTTACATCTTTTATTCTT

Product: protein of unknown function Met10

Products: S-adenosyl-L-homocysteine; DNA methylaminopurine [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTITEVETTITAKKVLCCKVPTNKGERIRKILLDNNELNKNYKLKKEDNYLFIPIISTDETEIDKSIMENLKKIYNNIEF
IEINMLGNPKTNKLNFREYLLKNFKKEIENGAVVLSYDIIGDIVIIQISDNIDENIAKKIGAKALELIPSVKSVYRRKSE
ILGEFRVRELELLAGEYKTLTMYKENGYRLWVDVEKVYFSPRLGWERKRIMEKVKPEDIVVDMFCGVGPFSIACKNAKKI
YAIDINPDAVELLKKNIKLNKLQHKIIPINNDIRKTEVKGNRIIMNLPKFAHLFVDTALKMVEKNGIIHYYTVGTDYNEA
INLFKSKCNCEIVEKRIVKSYSPREHIFVLDIKIY

Sequences:

>Translated_355_residues
MTITEVETTITAKKVLCCKVPTNKGERIRKILLDNNELNKNYKLKKEDNYLFIPIISTDETEIDKSIMENLKKIYNNIEF
IEINMLGNPKTNKLNFREYLLKNFKKEIENGAVVLSYDIIGDIVIIQISDNIDENIAKKIGAKALELIPSVKSVYRRKSE
ILGEFRVRELELLAGEYKTLTMYKENGYRLWVDVEKVYFSPRLGWERKRIMEKVKPEDIVVDMFCGVGPFSIACKNAKKI
YAIDINPDAVELLKKNIKLNKLQHKIIPINNDIRKTEVKGNRIIMNLPKFAHLFVDTALKMVEKNGIIHYYTVGTDYNEA
INLFKSKCNCEIVEKRIVKSYSPREHIFVLDIKIY
>Mature_354_residues
TITEVETTITAKKVLCCKVPTNKGERIRKILLDNNELNKNYKLKKEDNYLFIPIISTDETEIDKSIMENLKKIYNNIEFI
EINMLGNPKTNKLNFREYLLKNFKKEIENGAVVLSYDIIGDIVIIQISDNIDENIAKKIGAKALELIPSVKSVYRRKSEI
LGEFRVRELELLAGEYKTLTMYKENGYRLWVDVEKVYFSPRLGWERKRIMEKVKPEDIVVDMFCGVGPFSIACKNAKKIY
AIDINPDAVELLKKNIKLNKLQHKIIPINNDIRKTEVKGNRIIMNLPKFAHLFVDTALKMVEKNGIIHYYTVGTDYNEAI
NLFKSKCNCEIVEKRIVKSYSPREHIFVLDIKIY

Specific function: Unknown

COG id: COG2520

COG function: function code R; Predicted methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To M.jannaschii MJ1557 and to yeast YHR070w [H]

Homologues:

Organism=Homo sapiens, GI145275187, Length=306, Percent_Identity=28.7581699346405, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI193207537, Length=263, Percent_Identity=29.6577946768061, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193207541, Length=230, Percent_Identity=31.304347826087, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193207539, Length=230, Percent_Identity=31.304347826087, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321861, Length=209, Percent_Identity=28.2296650717703, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45551508, Length=201, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003402 [H]

Pfam domain/function: PF02475 Met_10 [H]

EC number: 2.1.1.72 [C]

Molecular weight: Translated: 41323; Mature: 41192

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00539 PYROKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITEVETTITAKKVLCCKVPTNKGERIRKILLDNNELNKNYKLKKEDNYLFIPIISTDE
CCEEEEHHHEEEEEEEEEECCCCCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCCH
TEIDKSIMENLKKIYNNIEFIEINMLGNPKTNKLNFREYLLKNFKKEIENGAVVLSYDII
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECC
GDIVIIQISDNIDENIAKKIGAKALELIPSVKSVYRRKSEILGEFRVRELELLAGEYKTL
CCEEEEEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TMYKENGYRLWVDVEKVYFSPRLGWERKRIMEKVKPEDIVVDMFCGVGPFSIACKNAKKI
EEEECCCEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEHHCCCCCCEEEECCCCEE
YAIDINPDAVELLKKNIKLNKLQHKIIPINNDIRKTEVKGNRIIMNLPKFAHLFVDTALK
EEEECCCHHHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCEEEEECCHHHHHHHHHHHH
MVEKNGIIHYYTVGTDYNEAINLFKSKCNCEIVEKRIVKSYSPREHIFVLDIKIY
HHHCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEEEC
>Mature Secondary Structure 
TITEVETTITAKKVLCCKVPTNKGERIRKILLDNNELNKNYKLKKEDNYLFIPIISTDE
CEEEEHHHEEEEEEEEEECCCCCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCCH
TEIDKSIMENLKKIYNNIEFIEINMLGNPKTNKLNFREYLLKNFKKEIENGAVVLSYDII
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECC
GDIVIIQISDNIDENIAKKIGAKALELIPSVKSVYRRKSEILGEFRVRELELLAGEYKTL
CCEEEEEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TMYKENGYRLWVDVEKVYFSPRLGWERKRIMEKVKPEDIVVDMFCGVGPFSIACKNAKKI
EEEECCCEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEHHCCCCCCEEEECCCCEE
YAIDINPDAVELLKKNIKLNKLQHKIIPINNDIRKTEVKGNRIIMNLPKFAHLFVDTALK
EEEECCCHHHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCEEEEECCHHHHHHHHHHHH
MVEKNGIIHYYTVGTDYNEAINLFKSKCNCEIVEKRIVKSYSPREHIFVLDIKIY
HHHCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; DNA adenine; DNA [C]

Specific reaction: S-Adenosyl-L-methionine + DNA adenine =S-adenosyl-L-homocysteine + DNA methylaminopurine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]