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The map label for this gene is sdhA [C]

Identifier: 150400681

GI number: 150400681

Start: 270853

End: 272511

Strand: Reverse

Name: sdhA [C]

Synonym: Maeo_0245

Alternate gene names: 150400681

Gene position: 272511-270853 (Counterclockwise)

Preceding gene: 150400682

Following gene: 150400678

Centisome position: 17.36

GC content: 33.15

Gene sequence:

>1659_bases
ATGATAACAGATATTTTAATTATTGGTGGCGGAGGAGCAGCGGCAAGATGTGCAATAGAATGCAATAAATCCGTAATAGT
GGCAGTTAAAGGACTTTTTGGTAAAAGTGGTTGCACTGTGATGGCAGAAGGAGGATATAACGCCGTTGTAAATCCAAATG
ACGATTTTGAAATTCATTTCAATGACACTATGAAAGGAGGAGCATACATAAATAATCCAAAATTGGTTGAAATACTTATA
AAAAATTCTCCAAAGGAATTAGAAAATTTAGAAAAATTTGGCTCACTATTCGATAGAAATGAGGACGGAACCATAGCACA
ACGACCATTTGGGGGGCAGAGTTTTAATAGAACTTGTTATAGTGGAGATAGCACAGGGCATGAAATAATGGCGGGGCTGA
TGGAATATGTTGGCAAAAAGGACAATATTAAGATTATGGAAGATACCACAGCAATAAAATTAATTGTAAAAAATAATGTT
TGTTATGGGGCTCTGTTTATAAACAACATCACAGGAGAAATATTCCCAATATATGCAAAATCCACAGTATTGGCAACGGG
CGGAGCTGGGCAATTATATCCAATTACATCAAATCCAATCCAAAAAGTAGGGGACGGTTTTGCACTGGCATATAATGAAG
GTGCAATTTTAAAAGATATGGAGATGGTGCAATTTCACCCAACGGGAATGGTTGGAACTGGTATATTGGTTACGGAAGCC
GTAAGGGGAGAAGGTGGAATATTATACAATAAAGATAAAGAAAGATTTATGAAAAATTACGACCCTCAAAGAATGGAGCT
ATCCACAAGAGATGTCGTAGCAAAAGCAATATTTAACGAGGTTCAAGAGGGAAGAGGAATAAACGGAGGAGTTTATTTAG
ATGTATCTCATCTCAACCCTAAATTAATAGAGGAAAAATTAGAAACAATGTTTAGGCAGTTTATGGATATTGGCGTAGAT
ATTAGAAAGGAACCAATGATTATAGCTCCTACGGCACATCATACAATGGGGGGCATAAGAATAAATGAAAAATGTGAAAC
CAATATAGGCGGATTATATGCCTGTGGAGAGGTTGCAGGCGGAATTCATGGGGCAAATAGATTGGGGGGAAATGCTCTGG
CAGATACTCAGGTTTTTGGAGCAATCGCTGGAAAAAATGCCTGTGATTATGCAGAAAATAGCCCTATAAATCATAAATTA
AATAATATCAATGAATATACAGAGGAATTAATTAATAATATTAAACAGGAAATAGATAATTTAAAAAATAAAGATAATGG
GGCTAATAAAAATAGTAAAGATAATAACACAAATGATGTGGAAACCGTATATAATTTAATAGATACACTTAAAAATATTA
TGTGGAATTATGTTTCCATAGTGAGAACAGAGGAAGGACTAAATAAGGCACTAAATGAAATAAATGATTTAAAAAATAAA
ATAGATAATGTAAAATTAAATGGTATAATTGATATTTTAAGGTATTTTGATTTAAAAAATATGATAGTAGTTAGTGAATT
AATTATAAAAAGTGCTTTAAAAAGAGAAGAAAGTAGGGGGGCCCATTATAGAAAAGATTTTCCTGAAACAATTGACAGAT
TTAAAGGCAATTTTGTAGCACAAAACAATAAAATTACTTTCATACCGTTAAAAGAATAA

Upstream 100 bases:

>100_bases
ACTATTTTTTAAATTAATTATATAATTATATATATTGTAATTAATAATATATATCCAAATGAAAGATAATTAAGGCAATT
AAGGCAATTAAGGTGATTTT

Downstream 100 bases:

>100_bases
AATAATATAAAAATAAATGAAAGAATGAATATAATAAAATAATAAAAATATTTACGCAAAATCTTTTAATTTTTATATCG
GAGCTCCGATAAAAAACACT

Product: succinate dehydrogenase flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MITDILIIGGGGAAARCAIECNKSVIVAVKGLFGKSGCTVMAEGGYNAVVNPNDDFEIHFNDTMKGGAYINNPKLVEILI
KNSPKELENLEKFGSLFDRNEDGTIAQRPFGGQSFNRTCYSGDSTGHEIMAGLMEYVGKKDNIKIMEDTTAIKLIVKNNV
CYGALFINNITGEIFPIYAKSTVLATGGAGQLYPITSNPIQKVGDGFALAYNEGAILKDMEMVQFHPTGMVGTGILVTEA
VRGEGGILYNKDKERFMKNYDPQRMELSTRDVVAKAIFNEVQEGRGINGGVYLDVSHLNPKLIEEKLETMFRQFMDIGVD
IRKEPMIIAPTAHHTMGGIRINEKCETNIGGLYACGEVAGGIHGANRLGGNALADTQVFGAIAGKNACDYAENSPINHKL
NNINEYTEELINNIKQEIDNLKNKDNGANKNSKDNNTNDVETVYNLIDTLKNIMWNYVSIVRTEEGLNKALNEINDLKNK
IDNVKLNGIIDILRYFDLKNMIVVSELIIKSALKREESRGAHYRKDFPETIDRFKGNFVAQNNKITFIPLKE

Sequences:

>Translated_552_residues
MITDILIIGGGGAAARCAIECNKSVIVAVKGLFGKSGCTVMAEGGYNAVVNPNDDFEIHFNDTMKGGAYINNPKLVEILI
KNSPKELENLEKFGSLFDRNEDGTIAQRPFGGQSFNRTCYSGDSTGHEIMAGLMEYVGKKDNIKIMEDTTAIKLIVKNNV
CYGALFINNITGEIFPIYAKSTVLATGGAGQLYPITSNPIQKVGDGFALAYNEGAILKDMEMVQFHPTGMVGTGILVTEA
VRGEGGILYNKDKERFMKNYDPQRMELSTRDVVAKAIFNEVQEGRGINGGVYLDVSHLNPKLIEEKLETMFRQFMDIGVD
IRKEPMIIAPTAHHTMGGIRINEKCETNIGGLYACGEVAGGIHGANRLGGNALADTQVFGAIAGKNACDYAENSPINHKL
NNINEYTEELINNIKQEIDNLKNKDNGANKNSKDNNTNDVETVYNLIDTLKNIMWNYVSIVRTEEGLNKALNEINDLKNK
IDNVKLNGIIDILRYFDLKNMIVVSELIIKSALKREESRGAHYRKDFPETIDRFKGNFVAQNNKITFIPLKE
>Mature_552_residues
MITDILIIGGGGAAARCAIECNKSVIVAVKGLFGKSGCTVMAEGGYNAVVNPNDDFEIHFNDTMKGGAYINNPKLVEILI
KNSPKELENLEKFGSLFDRNEDGTIAQRPFGGQSFNRTCYSGDSTGHEIMAGLMEYVGKKDNIKIMEDTTAIKLIVKNNV
CYGALFINNITGEIFPIYAKSTVLATGGAGQLYPITSNPIQKVGDGFALAYNEGAILKDMEMVQFHPTGMVGTGILVTEA
VRGEGGILYNKDKERFMKNYDPQRMELSTRDVVAKAIFNEVQEGRGINGGVYLDVSHLNPKLIEEKLETMFRQFMDIGVD
IRKEPMIIAPTAHHTMGGIRINEKCETNIGGLYACGEVAGGIHGANRLGGNALADTQVFGAIAGKNACDYAENSPINHKL
NNINEYTEELINNIKQEIDNLKNKDNGANKNSKDNNTNDVETVYNLIDTLKNIMWNYVSIVRTEEGLNKALNEINDLKNK
IDNVKLNGIIDILRYFDLKNMIVVSELIIKSALKREESRGAHYRKDFPETIDRFKGNFVAQNNKITFIPLKE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=540, Percent_Identity=34.0740740740741, Blast_Score=282, Evalue=6e-76,
Organism=Escherichia coli, GI1790597, Length=524, Percent_Identity=34.7328244274809, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1788928, Length=558, Percent_Identity=32.078853046595, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1786942, Length=537, Percent_Identity=34.4506517690875, Blast_Score=261, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17550100, Length=538, Percent_Identity=34.2007434944238, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17505833, Length=535, Percent_Identity=33.6448598130841, Blast_Score=268, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI71986328, Length=427, Percent_Identity=27.6346604215457, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322701, Length=538, Percent_Identity=34.2007434944238, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322416, Length=556, Percent_Identity=33.6330935251799, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6320788, Length=476, Percent_Identity=28.1512605042017, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322511, Length=413, Percent_Identity=27.3607748184019, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17137288, Length=533, Percent_Identity=33.0206378986867, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24655642, Length=533, Percent_Identity=33.0206378986867, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24655647, Length=533, Percent_Identity=33.0206378986867, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24663005, Length=537, Percent_Identity=31.8435754189944, Blast_Score=265, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 60892; Mature: 60892

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDILIIGGGGAAARCAIECNKSVIVAVKGLFGKSGCTVMAEGGYNAVVNPNDDFEIHF
CCEEEEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCEEEECCCCEEEECCCCCEEEEE
NDTMKGGAYINNPKLVEILIKNSPKELENLEKFGSLFDRNEDGTIAQRPFGGQSFNRTCY
CCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEE
SGDSTGHEIMAGLMEYVGKKDNIKIMEDTTAIKLIVKNNVCYGALFINNITGEIFPIYAK
CCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEEEEECCCEEEEEEEECCCCCEEEEEEC
STVLATGGAGQLYPITSNPIQKVGDGFALAYNEGAILKDMEMVQFHPTGMVGTGILVTEA
CEEEEECCCCEEEECCCCHHHHHCCCEEEEECCCCEEECCCEEEECCCCCCCCCEEEEEE
VRGEGGILYNKDKERFMKNYDPQRMELSTRDVVAKAIFNEVQEGRGINGGVYLDVSHLNP
ECCCCCEEECCCHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
KLIEEKLETMFRQFMDIGVDIRKEPMIIAPTAHHTMGGIRINEKCETNIGGLYACGEVAG
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCEEEHHHHHC
GIHGANRLGGNALADTQVFGAIAGKNACDYAENSPINHKLNNINEYTEELINNIKQEIDN
CCCCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LKNKDNGANKNSKDNNTNDVETVYNLIDTLKNIMWNYVSIVRTEEGLNKALNEINDLKNK
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNVKLNGIIDILRYFDLKNMIVVSELIIKSALKREESRGAHYRKDFPETIDRFKGNFVA
HCCEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHCCCEEE
QNNKITFIPLKE
ECCEEEEEECCC
>Mature Secondary Structure
MITDILIIGGGGAAARCAIECNKSVIVAVKGLFGKSGCTVMAEGGYNAVVNPNDDFEIHF
CCEEEEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCEEEECCCCEEEECCCCCEEEEE
NDTMKGGAYINNPKLVEILIKNSPKELENLEKFGSLFDRNEDGTIAQRPFGGQSFNRTCY
CCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEE
SGDSTGHEIMAGLMEYVGKKDNIKIMEDTTAIKLIVKNNVCYGALFINNITGEIFPIYAK
CCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEEEEECCCEEEEEEEECCCCCEEEEEEC
STVLATGGAGQLYPITSNPIQKVGDGFALAYNEGAILKDMEMVQFHPTGMVGTGILVTEA
CEEEEECCCCEEEECCCCHHHHHCCCEEEEECCCCEEECCCEEEECCCCCCCCCEEEEEE
VRGEGGILYNKDKERFMKNYDPQRMELSTRDVVAKAIFNEVQEGRGINGGVYLDVSHLNP
ECCCCCEEECCCHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
KLIEEKLETMFRQFMDIGVDIRKEPMIIAPTAHHTMGGIRINEKCETNIGGLYACGEVAG
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCEEEHHHHHC
GIHGANRLGGNALADTQVFGAIAGKNACDYAENSPINHKLNNINEYTEELINNIKQEIDN
CCCCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LKNKDNGANKNSKDNNTNDVETVYNLIDTLKNIMWNYVSIVRTEEGLNKALNEINDLKNK
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNVKLNGIIDILRYFDLKNMIVVSELIIKSALKREESRGAHYRKDFPETIDRFKGNFVA
HCCEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHCCCEEE
QNNKITFIPLKE
ECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.4 {fumarate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]