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The map label for this gene is vhcA [H]

Identifier: 150400535

GI number: 150400535

Start: 104119

End: 105546

Strand: Reverse

Name: vhcA [H]

Synonym: Maeo_0097

Alternate gene names: 150400535

Gene position: 105546-104119 (Counterclockwise)

Preceding gene: 150400536

Following gene: 150400534

Centisome position: 6.72

GC content: 35.01

Gene sequence:

>1428_bases
ATGGTAAAATTATCAGTTGAGCCTGTTACACGAGTTGAAGGACATGGCAAAATAACTGTGTCTTTTGACGAAAGTGGAGC
TTTGGATACAGTTCATTTTAATGTGGTGGAAGTAAGAGGTTTTGAAAAATTTATGGAAGGAAGATATGTGGAAGATGCAC
CAATATTGACCCCGAGAATCTGCGGTATATGTCAAGTTTCTCACCATTTAGCAAGTGCAAAAGCCGTGGATGAAATATAT
GGAGTAAAGATTCCAGAAACCGCAATGCTTTTAAGAAATTTGATGCATCAAAGTTCAACAGTTCATAGTCATGCCCTTCA
TTTTTATGCTCTTGCGGCACCTGATTTAATGTTTCCTACAACGGACGATATATTAAAAAGAAATTTAATGGGCATGGCAA
AAGAACATCCCGAAGTTGTTAAAGAAGCAATTGAATTAAGAAAAATAGGCCAAACTATTGTTAGAGTCGTCGGTGGAAGA
GCAATTCACCCCTGCACAGCAGTTGTTGGAGGGCAGTCCATATCATTAAGTGAAACTAAAAGGGATGAATTATTAAAATT
ATCAGATAGGGCGGTTGAGTTATCTGAAAAATCGATCGAAATTGGAAAAAAATTAATTGAAAATATAAAAAATAATGATT
TATTGGATATCGGATACTTTGAATCCTATCATGCCGGAATGGTAAATGATGGCACACATGAATTATATGATGGAAAAATT
AGAGTATGCGATTCAAAAGGAAAAACAGCTTATGAATATAATCCATCAGAATATTTAAACTATATTGCCGAAGGAGTTAA
ACCATATTCTTATTTAAAATTCCCATACTTAAAAGACCTCGGGGAAGAAGAAGGCGTTTATAGGGTAAATACCCTATCAA
GGCTCAATGTAGCGGACAGAATGGCAACACCCCTTGCACAAAAATATTATAAAGAATTTATCGAAGAATATGGAGAACCA
TGCCATTATCCGGTGCTATTTAATTATGCAAGATTAATAGAAATGCTTTCAAGTGCTGAACTAACGAAAAAATTATTAGA
AAATGATAAAATCGTTGATACCGATATAAAGGCAGAACCTAGTGGAGATGTAGTTGGCGAAGGTGTCGGATGTGTTGAAG
CACCAAGGGGGACATTATTCCACCACTTCAAAACAGATGATAAAGGAGTTATTACCGACACAAACTTAATTGTGGCAACA
ACTCAGAATAATCCTGCAATGGACATCGGAGTAAAAAAAGTAGCTGAAAAATATATAAAAACACCCGAAGATGCAACACC
AAATATTTTAAATTATATGGAAATGGTAATTAGAGCTTATGACCCCTGTTTATCTTGTGCCACACATGTTATAGGAGATT
CTGGTGGTAGTGGCCTTATATTGGAAACATATGAAGAAAATAAATTAATTAAATCAGTTCGAAATTAA

Upstream 100 bases:

>100_bases
TACAAATTTACATTACCTAGTGCTTTAATTCCAATTAAATTAAAAAGATAAATGGTAAAATTATAAAATAAATAAAATAA
ATAATATGTGGTGACATAAA

Downstream 100 bases:

>100_bases
TTAAAATTAATAAAATAAAAAAATTAGTAAAAATAATATATAAATATAAATGTGTGTCCGTAGGGATTAATTCATCTCTA
AAAAAATCAAACGGTGTGTT

Product: nickel-dependent hydrogenase large subunit

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MVKLSVEPVTRVEGHGKITVSFDESGALDTVHFNVVEVRGFEKFMEGRYVEDAPILTPRICGICQVSHHLASAKAVDEIY
GVKIPETAMLLRNLMHQSSTVHSHALHFYALAAPDLMFPTTDDILKRNLMGMAKEHPEVVKEAIELRKIGQTIVRVVGGR
AIHPCTAVVGGQSISLSETKRDELLKLSDRAVELSEKSIEIGKKLIENIKNNDLLDIGYFESYHAGMVNDGTHELYDGKI
RVCDSKGKTAYEYNPSEYLNYIAEGVKPYSYLKFPYLKDLGEEEGVYRVNTLSRLNVADRMATPLAQKYYKEFIEEYGEP
CHYPVLFNYARLIEMLSSAELTKKLLENDKIVDTDIKAEPSGDVVGEGVGCVEAPRGTLFHHFKTDDKGVITDTNLIVAT
TQNNPAMDIGVKKVAEKYIKTPEDATPNILNYMEMVIRAYDPCLSCATHVIGDSGGSGLILETYEENKLIKSVRN

Sequences:

>Translated_475_residues
MVKLSVEPVTRVEGHGKITVSFDESGALDTVHFNVVEVRGFEKFMEGRYVEDAPILTPRICGICQVSHHLASAKAVDEIY
GVKIPETAMLLRNLMHQSSTVHSHALHFYALAAPDLMFPTTDDILKRNLMGMAKEHPEVVKEAIELRKIGQTIVRVVGGR
AIHPCTAVVGGQSISLSETKRDELLKLSDRAVELSEKSIEIGKKLIENIKNNDLLDIGYFESYHAGMVNDGTHELYDGKI
RVCDSKGKTAYEYNPSEYLNYIAEGVKPYSYLKFPYLKDLGEEEGVYRVNTLSRLNVADRMATPLAQKYYKEFIEEYGEP
CHYPVLFNYARLIEMLSSAELTKKLLENDKIVDTDIKAEPSGDVVGEGVGCVEAPRGTLFHHFKTDDKGVITDTNLIVAT
TQNNPAMDIGVKKVAEKYIKTPEDATPNILNYMEMVIRAYDPCLSCATHVIGDSGGSGLILETYEENKLIKSVRN
>Mature_475_residues
MVKLSVEPVTRVEGHGKITVSFDESGALDTVHFNVVEVRGFEKFMEGRYVEDAPILTPRICGICQVSHHLASAKAVDEIY
GVKIPETAMLLRNLMHQSSTVHSHALHFYALAAPDLMFPTTDDILKRNLMGMAKEHPEVVKEAIELRKIGQTIVRVVGGR
AIHPCTAVVGGQSISLSETKRDELLKLSDRAVELSEKSIEIGKKLIENIKNNDLLDIGYFESYHAGMVNDGTHELYDGKI
RVCDSKGKTAYEYNPSEYLNYIAEGVKPYSYLKFPYLKDLGEEEGVYRVNTLSRLNVADRMATPLAQKYYKEFIEEYGEP
CHYPVLFNYARLIEMLSSAELTKKLLENDKIVDTDIKAEPSGDVVGEGVGCVEAPRGTLFHHFKTDDKGVITDTNLIVAT
TQNNPAMDIGVKKVAEKYIKTPEDATPNILNYMEMVIRAYDPCLSCATHVIGDSGGSGLILETYEENKLIKSVRN

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=115, Percent_Identity=35.6521739130435, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789368, Length=115, Percent_Identity=33.0434782608696, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 52944; Mature: 52944

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLSVEPVTRVEGHGKITVSFDESGALDTVHFNVVEVRGFEKFMEGRYVEDAPILTPRI
CEEEECCCHHEECCCCEEEEEECCCCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCHHH
CGICQVSHHLASAKAVDEIYGVKIPETAMLLRNLMHQSSTVHSHALHFYALAAPDLMFPT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
TDDILKRNLMGMAKEHPEVVKEAIELRKIGQTIVRVVGGRAIHPCTAVVGGQSISLSETK
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCEECCHHH
RDELLKLSDRAVELSEKSIEIGKKLIENIKNNDLLDIGYFESYHAGMVNDGTHELYDGKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCHHHCCCEE
RVCDSKGKTAYEYNPSEYLNYIAEGVKPYSYLKFPYLKDLGEEEGVYRVNTLSRLNVADR
EEECCCCCEEEECCHHHHHHHHHHCCCCHHHCCCHHHHHCCCCCCEEEECCHHHHHHHHH
MATPLAQKYYKEFIEEYGEPCHYPVLFNYARLIEMLSSAELTKKLLENDKIVDTDIKAEP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
SGDVVGEGVGCVEAPRGTLFHHFKTDDKGVITDTNLIVATTQNNPAMDIGVKKVAEKYIK
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHC
TPEDATPNILNYMEMVIRAYDPCLSCATHVIGDSGGSGLILETYEENKLIKSVRN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCC
>Mature Secondary Structure
MVKLSVEPVTRVEGHGKITVSFDESGALDTVHFNVVEVRGFEKFMEGRYVEDAPILTPRI
CEEEECCCHHEECCCCEEEEEECCCCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCHHH
CGICQVSHHLASAKAVDEIYGVKIPETAMLLRNLMHQSSTVHSHALHFYALAAPDLMFPT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
TDDILKRNLMGMAKEHPEVVKEAIELRKIGQTIVRVVGGRAIHPCTAVVGGQSISLSETK
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCEECCHHH
RDELLKLSDRAVELSEKSIEIGKKLIENIKNNDLLDIGYFESYHAGMVNDGTHELYDGKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCHHHCCCEE
RVCDSKGKTAYEYNPSEYLNYIAEGVKPYSYLKFPYLKDLGEEEGVYRVNTLSRLNVADR
EEECCCCCEEEECCHHHHHHHHHHCCCCHHHCCCHHHHHCCCCCCEEEECCHHHHHHHHH
MATPLAQKYYKEFIEEYGEPCHYPVLFNYARLIEMLSSAELTKKLLENDKIVDTDIKAEP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
SGDVVGEGVGCVEAPRGTLFHHFKTDDKGVITDTNLIVATTQNNPAMDIGVKKVAEKYIK
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHC
TPEDATPNILNYMEMVIRAYDPCLSCATHVIGDSGGSGLILETYEENKLIKSVRN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]