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The map label for this gene is vhuB [H]

Identifier: 150400534

GI number: 150400534

Start: 102864

End: 104015

Strand: Reverse

Name: vhuB [H]

Synonym: Maeo_0096

Alternate gene names: 150400534

Gene position: 104015-102864 (Counterclockwise)

Preceding gene: 150400535

Following gene: 150400533

Centisome position: 6.63

GC content: 35.68

Gene sequence:

>1152_bases
TTGGAAGGAATTAAGATTCAAGAAGATGCATGTTTAGTATGTGATGCATGTAAAGCCGTGTGTCCAACTGACGCCATAGA
AATATCACCATTTAATAGTTGTAATCTATGCATGCAATGTGTAAAAGTATGTCCAACCAAGGCATTATCTGAGGTGGCAG
GTAAGATATCATATAACTCCTCAATATGTAAAAAATGCGGAGCATGTGCTACTGCATGTCCGACAAAAATTAAAAAAGTA
GATAATATATTCCCATATTCAAAAGGACATTGTATTTTATGCGAAATGTGTGTGGAAGTATGCCCAATTGAAATCATATC
AATTCCAACAATTATTGATAAACCTAAAAAAGAAATTATTATTCCAAAGGACCCAATAGCCGTAGTTGATGCCTGTGTTG
GGTGTGGAGTATGTGTTCCAGTATGTCCCGTAGAAGCCATAAATATTGAGAATGACAGAGCAGTTGTGGATAAAAATAAA
TGTATGTATTGCTCAATATGTGCCCAAACATGCCCATGGAATGCCATATATGTAGCAGGCGAAATTCCTAAGAAAAGACA
TAAAGAGCAGATATTTACTATGGCAGAAGATAAATGTATTGGATGTATGGCATGCGCCGAAATATGCCCTGGAAGTATGA
TATCACCAGATGTCGATAATTTAAAAGTAGAACTGCCAAAAGTATGTCCTGCATGTGGCTTATGTGTTGAGGTTTGTCCA
GTTGATGCTTTATCACTTGATGTTAAGTATGAATCGGCCCACCCAATAACTGATGAAGGCATTGTAATAATTGAAGACGA
TTATGATATATTAGAAAAATGTGCAAATGTTTGTCCTACTGATGCCATAATTGCCGAGAGTGAATCAAAATCCGTAAAAA
TGTGCATTGTATGCGGAGCATGTGTTACAGCTTGTCCCACAGGAGCATTAAAAATTGGCACGATAAATCACAATGGAAAA
GAATATAATAGGATAGAATATGGGCCCCACCTATGCGATAAATGTGGAAAATGTGTATCTACATGTCCAATGAAGACTTT
AAGATTAACTGATGATAAAATATCATTAAAAGGATATTGTGTTATGTGTCTGCTCTGTCTTTCATGTGCAGAAAGTCTAA
AAAAAGATAAAGACATTTTGAGATTGAAATGA

Upstream 100 bases:

>100_bases
AAATTAATAAAATAAAAAAATTAGTAAAAATAATATATAAATATAAATGTGTGTCCGTAGGGATTAATTCATCTCTAAAA
AAATCAAACGGTGTGTTAAA

Downstream 100 bases:

>100_bases
TTTATTTTTTAAAATATGGTATTAAGAAATGTAATGTGGATATAACTAATATAAATATAGATATTAATAATACAATATAT
TTATAGGGTGAATAATATGG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MEGIKIQEDACLVCDACKAVCPTDAIEISPFNSCNLCMQCVKVCPTKALSEVAGKISYNSSICKKCGACATACPTKIKKV
DNIFPYSKGHCILCEMCVEVCPIEIISIPTIIDKPKKEIIIPKDPIAVVDACVGCGVCVPVCPVEAINIENDRAVVDKNK
CMYCSICAQTCPWNAIYVAGEIPKKRHKEQIFTMAEDKCIGCMACAEICPGSMISPDVDNLKVELPKVCPACGLCVEVCP
VDALSLDVKYESAHPITDEGIVIIEDDYDILEKCANVCPTDAIIAESESKSVKMCIVCGACVTACPTGALKIGTINHNGK
EYNRIEYGPHLCDKCGKCVSTCPMKTLRLTDDKISLKGYCVMCLLCLSCAESLKKDKDILRLK

Sequences:

>Translated_383_residues
MEGIKIQEDACLVCDACKAVCPTDAIEISPFNSCNLCMQCVKVCPTKALSEVAGKISYNSSICKKCGACATACPTKIKKV
DNIFPYSKGHCILCEMCVEVCPIEIISIPTIIDKPKKEIIIPKDPIAVVDACVGCGVCVPVCPVEAINIENDRAVVDKNK
CMYCSICAQTCPWNAIYVAGEIPKKRHKEQIFTMAEDKCIGCMACAEICPGSMISPDVDNLKVELPKVCPACGLCVEVCP
VDALSLDVKYESAHPITDEGIVIIEDDYDILEKCANVCPTDAIIAESESKSVKMCIVCGACVTACPTGALKIGTINHNGK
EYNRIEYGPHLCDKCGKCVSTCPMKTLRLTDDKISLKGYCVMCLLCLSCAESLKKDKDILRLK
>Mature_383_residues
MEGIKIQEDACLVCDACKAVCPTDAIEISPFNSCNLCMQCVKVCPTKALSEVAGKISYNSSICKKCGACATACPTKIKKV
DNIFPYSKGHCILCEMCVEVCPIEIISIPTIIDKPKKEIIIPKDPIAVVDACVGCGVCVPVCPVEAINIENDRAVVDKNK
CMYCSICAQTCPWNAIYVAGEIPKKRHKEQIFTMAEDKCIGCMACAEICPGSMISPDVDNLKVELPKVCPACGLCVEVCP
VDALSLDVKYESAHPITDEGIVIIEDDYDILEKCANVCPTDAIIAESESKSVKMCIVCGACVTACPTGALKIGTINHNGK
EYNRIEYGPHLCDKCGKCVSTCPMKTLRLTDDKISLKGYCVMCLLCLSCAESLKKDKDILRLK

Specific function: Probable Electron Transfer Protein For Hydrogenase 4. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41359; Mature: 41359

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

12.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
14.6 %Cys+Met (Translated Protein)
12.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
14.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGIKIQEDACLVCDACKAVCPTDAIEISPFNSCNLCMQCVKVCPTKALSEVAGKISYNS
CCCCEECCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCH
SICKKCGACATACPTKIKKVDNIFPYSKGHCILCEMCVEVCPIEIISIPTIIDKPKKEII
HHHHHHCCCHHHCHHHHHHHHHCCCCCCCCEEHHHHHHHHCCEEEEECCHHHCCCCCCEE
IPKDPIAVVDACVGCGVCVPVCPVEAINIENDRAVVDKNKCMYCSICAQTCPWNAIYVAG
CCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCEEEEEEHHHHCCCCEEEEEC
EIPKKRHKEQIFTMAEDKCIGCMACAEICPGSMISPDVDNLKVELPKVCPACGLCVEVCP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHCCHHCCHHHHCC
VDALSLDVKYESAHPITDEGIVIIEDDYDILEKCANVCPTDAIIAESESKSVKMCIVCGA
CCEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECH
CVTACPTGALKIGTINHNGKEYNRIEYGPHLCDKCGKCVSTCPMKTLRLTDDKISLKGYC
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
VMCLLCLSCAESLKKDKDILRLK
HHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MEGIKIQEDACLVCDACKAVCPTDAIEISPFNSCNLCMQCVKVCPTKALSEVAGKISYNS
CCCCEECCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCH
SICKKCGACATACPTKIKKVDNIFPYSKGHCILCEMCVEVCPIEIISIPTIIDKPKKEII
HHHHHHCCCHHHCHHHHHHHHHCCCCCCCCEEHHHHHHHHCCEEEEECCHHHCCCCCCEE
IPKDPIAVVDACVGCGVCVPVCPVEAINIENDRAVVDKNKCMYCSICAQTCPWNAIYVAG
CCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCEEEEEEHHHHCCCCEEEEEC
EIPKKRHKEQIFTMAEDKCIGCMACAEICPGSMISPDVDNLKVELPKVCPACGLCVEVCP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHCCHHCCHHHHCC
VDALSLDVKYESAHPITDEGIVIIEDDYDILEKCANVCPTDAIIAESESKSVKMCIVCGA
CCEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECH
CVTACPTGALKIGTINHNGKEYNRIEYGPHLCDKCGKCVSTCPMKTLRLTDDKISLKGYC
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
VMCLLCLSCAESLKKDKDILRLK
HHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]