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The map label for this gene is fwdB [H]

Identifier: 150400523

GI number: 150400523

Start: 87937

End: 89280

Strand: Direct

Name: fwdB [H]

Synonym: Maeo_0085

Alternate gene names: 150400523

Gene position: 87937-89280 (Clockwise)

Preceding gene: 150400522

Following gene: 150400525

Centisome position: 5.6

GC content: 37.28

Gene sequence:

>1344_bases
ATGAAAAAAGTATATAGGGATATCAACTGTCCCGTATGTGGTATGGGATGTGATGATATCGTAGTAATCTACGATAAAGA
AAACAATACGATAGAGACAGAAAATGTATGTAAAGAGGGAGCTCCTAAATTCATGGAAGTTGTAAGTTCCCACAGATTAA
GAAAACCATTAATTAAAAAAGATAAAGACTTTGAAGAAGTAGAATGGGAAAAGGCAATAGACGAAGCTGCTAAGGTTTTG
GCTAAGGCTAAAAGACCTTTATTGTTCCTTGGTGCTGAAACCTCCTCAGAGGCACAAATTGTAGGATTGCATATGGCAGA
ATATTTGGGAGGAATAGCAGATTCAAATTCAACAATATGACACGGTCCAACATTAATGGGTGTGCAAGAGGCTGGGCTAC
CTACTTGTACAGCAGGTGAAGTTAAAAATAGGGCAGATTTACTAATATATTGGGGCTGTAATCCAATGCACTCTCACCCA
AGACAGATAAGTAGATACTCTGGATTTGCCAGAGGATACTTTACTGAAAGGGGTATGAAAGATAGAAAAATGGTTGTTGC
CGATCCAAGGGTAACAGATACGGCAAAATTGGCTGATGTGCATTTCCAATTAAAACCAAATAGTGATTATGAGTTATTCT
CGGCACTGTTGATAGTGTTAAGAGGAAAAAAACCACATCCAAGTGTGGAGGAAATCACTGGTATTACAATAGAACAGATG
GAAGCAGCCATAGAACTCATAAAAGAATCAAAATATGTAATGATTTATGGCGGATTGGGGCTTCCAGCATCAAAAGGTAG
CCACAGGAATATTGAAATGGTTTTAAGATTGACAAATGAAATAAACAAATATACAAAATGTAATATTGGAGCTCTTAGAG
GGCACTGTAATGTAAATGGATTTAACCAGATTGCTTCATATATGTATGGTTATCCATATGGTTTGGATTTTTCAAGAGGT
TATCCAAGATATAACCCAGGAGAATTCACGGCTACAGATGTGCTGAGAGATGGGGAAGTAGATGCCATATTTTTAATGGC
TGCTGATCTTGGTGCCCATATGCCGAGAGATTGCGTTAAAAAAATAAATGATATCCCCATGGTATGTATTGATATTGCCC
CTGGGCCATCTACGACCTCCGCCGATGTGGTATTGCCCGGAGTTATAGATGCTATGGAGTGTGATGCTACATTTTATAGG
TTTGATGGTTTGACACAATATGCTCAGCCATTTACAGAATCACCATTTTCATTTACCAAAAGTAATGAGGATACTGTAAA
ACAGTTATTCGAAAAAGTAAAAGAATACAAAGAAAAAATGAAAAATGGCGAAGAAATAGAATAA

Upstream 100 bases:

>100_bases
TCTCCCCTGTATAAAGGAAATAGTGTATTTGTTGAACCGATTGAAAAAGGAAATATAATCGATGCAGAACAGATGATAAA
AAGTATGTATTGGTGAGATA

Downstream 100 bases:

>100_bases
ATAAAATAGAACAAATAAGAAATAAAATAATAATAACAATAAAAAGGATTACAAAAGCCCAAGCCCTTAAATATATATTA
AATTACATATAATGATACCC

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: Tungsten-containing formylmethanofuran dehydrogenase II subunit B [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKKVYRDINCPVCGMGCDDIVVIYDKENNTIETENVCKEGAPKFMEVVSSHRLRKPLIKKDKDFEEVEWEKAIDEAAKVL
AKAKRPLLFLGAETSSEAQIVGLHMAEYLGGIADSNSTIUHGPTLMGVQEAGLPTCTAGEVKNRADLLIYWGCNPMHSHP
RQISRYSGFARGYFTERGMKDRKMVVADPRVTDTAKLADVHFQLKPNSDYELFSALLIVLRGKKPHPSVEEITGITIEQM
EAAIELIKESKYVMIYGGLGLPASKGSHRNIEMVLRLTNEINKYTKCNIGALRGHCNVNGFNQIASYMYGYPYGLDFSRG
YPRYNPGEFTATDVLRDGEVDAIFLMAADLGAHMPRDCVKKINDIPMVCIDIAPGPSTTSADVVLPGVIDAMECDATFYR
FDGLTQYAQPFTESPFSFTKSNEDTVKQLFEKVKEYKEKMKNGEEIE

Sequences:

>Translated_447_residues
MKKVYRDINCPVCGMGCDDIVVIYDKENNTIETENVCKEGAPKFMEVVSSHRLRKPLIKKDKDFEEVEWEKAIDEAAKVL
AKAKRPLLFLGAETSSEAQIVGLHMAEYLGGIADSNSTI*HGPTLMGVQEAGLPTCTAGEVKNRADLLIYWGCNPMHSHP
RQISRYSGFARGYFTERGMKDRKMVVADPRVTDTAKLADVHFQLKPNSDYELFSALLIVLRGKKPHPSVEEITGITIEQM
EAAIELIKESKYVMIYGGLGLPASKGSHRNIEMVLRLTNEINKYTKCNIGALRGHCNVNGFNQIASYMYGYPYGLDFSRG
YPRYNPGEFTATDVLRDGEVDAIFLMAADLGAHMPRDCVKKINDIPMVCIDIAPGPSTTSADVVLPGVIDAMECDATFYR
FDGLTQYAQPFTESPFSFTKSNEDTVKQLFEKVKEYKEKMKNGEEIE
>Mature_447_residues
MKKVYRDINCPVCGMGCDDIVVIYDKENNTIETENVCKEGAPKFMEVVSSHRLRKPLIKKDKDFEEVEWEKAIDEAAKVL
AKAKRPLLFLGAETSSEAQIVGLHMAEYLGGIADSNSTI*HGPTLMGVQEAGLPTCTAGEVKNRADLLIYWGCNPMHSHP
RQISRYSGFARGYFTERGMKDRKMVVADPRVTDTAKLADVHFQLKPNSDYELFSALLIVLRGKKPHPSVEEITGITIEQM
EAAIELIKESKYVMIYGGLGLPASKGSHRNIEMVLRLTNEINKYTKCNIGALRGHCNVNGFNQIASYMYGYPYGLDFSRG
YPRYNPGEFTATDVLRDGEVDAIFLMAADLGAHMPRDCVKKINDIPMVCIDIAPGPSTTSADVVLPGVIDAMECDATFYR
FDGLTQYAQPFTESPFSFTKSNEDTVKQLFEKVKEYKEKMKNGEEIE

Specific function: Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile [H]

COG id: COG1029

COG function: function code C; Formylmethanofuran dehydrogenase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fwdB family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=482, Percent_Identity=22.8215767634855, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016457
- InterPro:   IPR006656 [H]

Pfam domain/function: PF00384 Molybdopterin [H]

EC number: =1.2.99.5 [H]

Molecular weight: Translated: 49794; Mature: 49794

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVYRDINCPVCGMGCDDIVVIYDKENNTIETENVCKEGAPKFMEVVSSHRLRKPLIKK
CCCCHHCCCCCEECCCCCCEEEEEECCCCEECHHHHHHHCCHHHHHHHHHHHHHHHHHHC
DKDFEEVEWEKAIDEAAKVLAKAKRPLLFLGAETSSEAQIVGLHMAEYLGGIADSNSTIH
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEHHHHHCCCCCCCCEEC
GPTLMGVQEAGLPTCTAGEVKNRADLLIYWGCNPMHSHPRQISRYSGFARGYFTERGMKD
CCEEECHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHCCCCC
RKMVVADPRVTDTAKLADVHFQLKPNSDYELFSALLIVLRGKKPHPSVEEITGITIEQME
CEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHH
AAIELIKESKYVMIYGGLGLPASKGSHRNIEMVLRLTNEINKYTKCNIGALRGHCNVNGF
HHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
NQIASYMYGYPYGLDFSRGYPRYNPGEFTATDVLRDGEVDAIFLMAADLGAHMPRDCVKK
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEEEHHCCCCCHHHHHH
INDIPMVCIDIAPGPSTTSADVVLPGVIDAMECDATFYRFDGLTQYAQPFTESPFSFTKS
HCCCCEEEEEECCCCCCCCCCEEECCHHHHHHCCCEEEECCCHHHHHCCCCCCCCCCCCC
NEDTVKQLFEKVKEYKEKMKNGEEIE
CHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKVYRDINCPVCGMGCDDIVVIYDKENNTIETENVCKEGAPKFMEVVSSHRLRKPLIKK
CCCCHHCCCCCEECCCCCCEEEEEECCCCEECHHHHHHHCCHHHHHHHHHHHHHHHHHHC
DKDFEEVEWEKAIDEAAKVLAKAKRPLLFLGAETSSEAQIVGLHMAEYLGGIADSNSTIH
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEHHHHHCCCCCCCCEEC
GPTLMGVQEAGLPTCTAGEVKNRADLLIYWGCNPMHSHPRQISRYSGFARGYFTERGMKD
CCEEECHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHCCCCC
RKMVVADPRVTDTAKLADVHFQLKPNSDYELFSALLIVLRGKKPHPSVEEITGITIEQME
CEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHH
AAIELIKESKYVMIYGGLGLPASKGSHRNIEMVLRLTNEINKYTKCNIGALRGHCNVNGF
HHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
NQIASYMYGYPYGLDFSRGYPRYNPGEFTATDVLRDGEVDAIFLMAADLGAHMPRDCVKK
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEEEHHCCCCCHHHHHH
INDIPMVCIDIAPGPSTTSADVVLPGVIDAMECDATFYRFDGLTQYAQPFTESPFSFTKS
HCCCCEEEEEECCCCCCCCCCEEECCHHHHHHCCCEEEECCCHHHHHCCCCCCCCCCCCC
NEDTVKQLFEKVKEYKEKMKNGEEIE
CHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]