Definition Methanococcus aeolicus Nankai-3, complete genome.
Accession NC_009635
Length 1,569,500

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The map label for this gene is hisS

Identifier: 150400469

GI number: 150400469

Start: 32749

End: 34005

Strand: Direct

Name: hisS

Synonym: Maeo_0030

Alternate gene names: 150400469

Gene position: 32749-34005 (Clockwise)

Preceding gene: 150400468

Following gene: 150400472

Centisome position: 2.09

GC content: 29.28

Gene sequence:

>1257_bases
ATGTTTAACAGACCAAAAGGAACAAGAGATTTTGCACCAAGTGAAATGAAAAAAAGAAAAATAATTGAAAATAAATTAAA
AAGCGTATTTATGGCATATAATTTCAATGAGATAAACACCCCCACATTTGAATATTTTGAATTATTATCTAAAAAAACAG
GGGAAGACATAAGAAAACAATTATTTGTATTTAAAGACCATGGAAATAGGGAAATGGGACTTAGACCAGAAATAACCTCC
TCAGTGGTTAGATTTTATATAAATGAGCTCAAAAATCTCCCGAAACCTCAAAAATTATTTTATTTTGCCAACTGTTTTAG
GTATGAACAGCCACAGGCAGGAAGATATAGGGAATTTTGGCAAATGGGATGCGAGTTATTGGGTAGTAAAAATCCCATAG
CAGATGCAGAAGTTATAAATTTAGCTATGGACGGATTGAATAAAATAAATATGGATTATGAAATACACATAGGACATTTG
GGAGTTTTAAAAGGTGTATTTGAAGAATTTGGGCTTACAGAAGATGAAGAATTAAAAATAAGGCGATTAATCGATAAAGA
AGATTATGAGAATTTAGAAAAATGTTTAATTGAATTGGAGGAAAACAAAAATATAATTATAAAAGATATTATATTCTCTG
TTTTAAACTCAAAGGGGACTGTGGAGGAAGTAATTGGTAATTTAAAAGAAATATTGAAAAATTACCCAAAATCAATTGAG
GCACTGAATAATTTAGAAGAAATTATGGAATTTGTAAAACGAAATAACTATATTATAAATTTAGGAATAGCAAGGGGATT
GGACTATTATACTGGAATGGTATTTGAAGTATATGGTAAAAAAGAAGGAGCTCGGCAGGTATGCGGTGGCGGTAGATATG
ACAATTTAATAGAATTATTTGAGGGAGCTCCCACGCCTGCGGTAGGTTTTGCCTACGGATTTGATAGAATTATGTTAAAT
ATTGACGATTTTGAAGTTGAAGATAAACGAATATTGATAGTACCAATTAAAAAAGATAAAAAACTTTTAAAGGAATGTTT
AATCATTGCCGATAAATTAAGAGAAATGGAAAAAATTGTAGAAGTAGATTTAATGAATAGAAAATTAAATAAAGCTTTGA
ATTATGCCAATACCGTTGGAATTAGTAAGGTAATAATGATAGGGGAAAAAGAATTAAATGAACGAAAAATTTCCATAAAA
GATATGGAAACAGGAGAACAAAAATTGATTGATTTGGAGGAATTAAGCAATATTTAA

Upstream 100 bases:

>100_bases
ATTCCTACGGAATTCACAAATCTTTGATTTGGGTAGGTTATTTTATATATTTATTTTATATATTTTATTTTTTATCATAT
CAAACCGAACAGGTGGAATA

Downstream 100 bases:

>100_bases
TTTTTTTTATTTTTTTATTTCACTTTTGTATTTTAGTAGATAAGTATGTCAAAACTGTATATCTATATAGTATGTGTACT
TATAGTCCAAGTTCTTTTTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MFNRPKGTRDFAPSEMKKRKIIENKLKSVFMAYNFNEINTPTFEYFELLSKKTGEDIRKQLFVFKDHGNREMGLRPEITS
SVVRFYINELKNLPKPQKLFYFANCFRYEQPQAGRYREFWQMGCELLGSKNPIADAEVINLAMDGLNKINMDYEIHIGHL
GVLKGVFEEFGLTEDEELKIRRLIDKEDYENLEKCLIELEENKNIIIKDIIFSVLNSKGTVEEVIGNLKEILKNYPKSIE
ALNNLEEIMEFVKRNNYIINLGIARGLDYYTGMVFEVYGKKEGARQVCGGGRYDNLIELFEGAPTPAVGFAYGFDRIMLN
IDDFEVEDKRILIVPIKKDKKLLKECLIIADKLREMEKIVEVDLMNRKLNKALNYANTVGISKVIMIGEKELNERKISIK
DMETGEQKLIDLEELSNI

Sequences:

>Translated_418_residues
MFNRPKGTRDFAPSEMKKRKIIENKLKSVFMAYNFNEINTPTFEYFELLSKKTGEDIRKQLFVFKDHGNREMGLRPEITS
SVVRFYINELKNLPKPQKLFYFANCFRYEQPQAGRYREFWQMGCELLGSKNPIADAEVINLAMDGLNKINMDYEIHIGHL
GVLKGVFEEFGLTEDEELKIRRLIDKEDYENLEKCLIELEENKNIIIKDIIFSVLNSKGTVEEVIGNLKEILKNYPKSIE
ALNNLEEIMEFVKRNNYIINLGIARGLDYYTGMVFEVYGKKEGARQVCGGGRYDNLIELFEGAPTPAVGFAYGFDRIMLN
IDDFEVEDKRILIVPIKKDKKLLKECLIIADKLREMEKIVEVDLMNRKLNKALNYANTVGISKVIMIGEKELNERKISIK
DMETGEQKLIDLEELSNI
>Mature_418_residues
MFNRPKGTRDFAPSEMKKRKIIENKLKSVFMAYNFNEINTPTFEYFELLSKKTGEDIRKQLFVFKDHGNREMGLRPEITS
SVVRFYINELKNLPKPQKLFYFANCFRYEQPQAGRYREFWQMGCELLGSKNPIADAEVINLAMDGLNKINMDYEIHIGHL
GVLKGVFEEFGLTEDEELKIRRLIDKEDYENLEKCLIELEENKNIIIKDIIFSVLNSKGTVEEVIGNLKEILKNYPKSIE
ALNNLEEIMEFVKRNNYIINLGIARGLDYYTGMVFEVYGKKEGARQVCGGGRYDNLIELFEGAPTPAVGFAYGFDRIMLN
IDDFEVEDKRILIVPIKKDKKLLKECLIIADKLREMEKIVEVDLMNRKLNKALNYANTVGISKVIMIGEKELNERKISIK
DMETGEQKLIDLEELSNI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=449, Percent_Identity=28.2850779510022, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI15029520, Length=441, Percent_Identity=27.891156462585, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1788861, Length=432, Percent_Identity=28.9351851851852, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71993686, Length=449, Percent_Identity=26.2806236080178, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71993693, Length=449, Percent_Identity=26.2806236080178, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325290, Length=356, Percent_Identity=28.6516853932584, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24643061, Length=465, Percent_Identity=27.5268817204301, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24643059, Length=484, Percent_Identity=26.4462809917355, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_META3 (A6UT01)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324235.1
- ProteinModelPortal:   A6UT01
- SMR:   A6UT01
- STRING:   A6UT01
- GeneID:   5327007
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0030
- eggNOG:   arNOG04127
- HOGENOM:   HBG616575
- OMA:   FDMSIVR
- ProtClustDB:   PRK00037
- BioCyc:   MAEO419665:MAEO_0030-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48545; Mature: 48545

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MFNRPKGTRDFAPSEMKKRKIIENKLKSVFMAYNFNEINTPTFEYFELLSKKTGEDIRKQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LFVFKDHGNREMGLRPEITSSVVRFYINELKNLPKPQKLFYFANCFRYEQPQAGRYREFW
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
QMGCELLGSKNPIADAEVINLAMDGLNKINMDYEIHIGHLGVLKGVFEEFGLTEDEELKI
HHHHHHHCCCCCCCHHHHHHHHHHCHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHH
RRLIDKEDYENLEKCLIELEENKNIIIKDIIFSVLNSKGTVEEVIGNLKEILKNYPKSIE
HHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
ALNNLEEIMEFVKRNNYIINLGIARGLDYYTGMVFEVYGKKEGARQVCGGGRYDNLIELF
HHHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHEEEECCCCCHHHHCCCCCHHHHHHHH
EGAPTPAVGFAYGFDRIMLNIDDFEVEDKRILIVPIKKDKKLLKECLIIADKLREMEKIV
CCCCCCCHHHHHCCHHEEEECCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EVDLMNRKLNKALNYANTVGISKVIMIGEKELNERKISIKDMETGEQKLIDLEELSNI
HHHHHHHHHHHHHHHHHHCCHHHEEEECCHHCCCCEEEEECCCCCHHHHCCHHHHCCC
>Mature Secondary Structure
MFNRPKGTRDFAPSEMKKRKIIENKLKSVFMAYNFNEINTPTFEYFELLSKKTGEDIRKQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LFVFKDHGNREMGLRPEITSSVVRFYINELKNLPKPQKLFYFANCFRYEQPQAGRYREFW
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
QMGCELLGSKNPIADAEVINLAMDGLNKINMDYEIHIGHLGVLKGVFEEFGLTEDEELKI
HHHHHHHCCCCCCCHHHHHHHHHHCHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHH
RRLIDKEDYENLEKCLIELEENKNIIIKDIIFSVLNSKGTVEEVIGNLKEILKNYPKSIE
HHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
ALNNLEEIMEFVKRNNYIINLGIARGLDYYTGMVFEVYGKKEGARQVCGGGRYDNLIELF
HHHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHEEEECCCCCHHHHCCCCCHHHHHHHH
EGAPTPAVGFAYGFDRIMLNIDDFEVEDKRILIVPIKKDKKLLKECLIIADKLREMEKIV
CCCCCCCHHHHHCCHHEEEECCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EVDLMNRKLNKALNYANTVGISKVIMIGEKELNERKISIKDMETGEQKLIDLEELSNI
HHHHHHHHHHHHHHHHHHCCHHHEEEECCHHCCCCEEEEECCCCCHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA