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The map label for this gene is purA

Identifier: 150400472

GI number: 150400472

Start: 36500

End: 37510

Strand: Direct

Name: purA

Synonym: Maeo_0033

Alternate gene names: 150400472

Gene position: 36500-37510 (Clockwise)

Preceding gene: 150400469

Following gene: 150400473

Centisome position: 2.33

GC content: 36.2

Gene sequence:

>1011_bases
ATGACATGCACCATAGTAGTCGGCGGTCAGTGGGGCGACGAAGGAAAAGGAAAAATAATTAGTTATATATGTGAAAAAGA
TAAGCCATCAGTAGTGGCAAGGGGCGGAGTAGGTCCCAATGCAGGGCATAGTGTAGAAGTTGATGGTAAAAAATACGGAA
TAAGAATGGTTCCAACAGGATTTCCAAACAGGAGCTCCAAACTTGCAGTAGGTGCAGGTGTATTAGTTGATGTTGATGTT
TTATTAAAAGAAGTTGAAATGTTAAAGGAATTTAATATCAAAGATAGGGTAATCATTGATTACAATTGTGGAGTGATTGA
GGAAAAACATAAAGAAATGGATAAATCAAACTCCCATTTATCAAAAGAAATAGGCTCCACAGGAACAGGATGCGGTCCTG
CAAATGTAGATAGGGCAATGAGAACGCTGAGATTGGCAAAAGAATTTGATGCTTTAAAACCTTTTTTAGGGGATGTTTCA
GACCTAGTAAATGAAGCCCTTGAAAATGGAGAAAATGTATTGATTGAGGGAACACAGGGAACATTATTATCCTTATATTA
TGGTAGTTATCCATATGTTACCTCAAATGACACCTCTGCATCAGCATTTGCTTCTGATGTGGGTATTGGACCTACAAAAG
TTGATGAAGTAATAGTTGTATTCAAATCATACCCTACAAGAGTTGGAGAAGGTCCATTCCCTACTGAAATGGATGTAGAA
GAGGCTGAAAAATTAGGACTTGTAGAATATGGAACTGTTACAGGTAGAAGAAGGAGAATAGGATATTTTGATTATGATTT
AGCAAAAAAAGTATGTAGACTAAATGGAGCTACGGCAATTGCTTTAACATGCCTTGATAAATATGATGAAGAATGTCATG
GACTTACTAACTATGACGACATCACGGAAAAAGGATTGGCATTTATAAAAGAAATTGAAGAAAAAGTTGGAGTTCCAGTT
AAAATTATCTCAACAGGTCCAGAACTTCATCAAACTATTGATATAAGATAA

Upstream 100 bases:

>100_bases
ATATCGTCGTGTTAAACCTATTAGAGGTTAAATATAATAAAAATCGACAAACATCGTTATAAATAAAGTTGTCGATTGAC
TCTATAAGGAGGTCTAATAT

Downstream 100 bases:

>100_bases
TTAAGACAATAATTTTAATATATTTTTATTTTTTATCTGTTTTTTATTATTTTTATTATAATATTATTTTTTAATTTTTG
CTCAATTTTCCCATTTTAAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTCTIVVGGQWGDEGKGKIISYICEKDKPSVVARGGVGPNAGHSVEVDGKKYGIRMVPTGFPNRSSKLAVGAGVLVDVDV
LLKEVEMLKEFNIKDRVIIDYNCGVIEEKHKEMDKSNSHLSKEIGSTGTGCGPANVDRAMRTLRLAKEFDALKPFLGDVS
DLVNEALENGENVLIEGTQGTLLSLYYGSYPYVTSNDTSASAFASDVGIGPTKVDEVIVVFKSYPTRVGEGPFPTEMDVE
EAEKLGLVEYGTVTGRRRRIGYFDYDLAKKVCRLNGATAIALTCLDKYDEECHGLTNYDDITEKGLAFIKEIEEKVGVPV
KIISTGPELHQTIDIR

Sequences:

>Translated_336_residues
MTCTIVVGGQWGDEGKGKIISYICEKDKPSVVARGGVGPNAGHSVEVDGKKYGIRMVPTGFPNRSSKLAVGAGVLVDVDV
LLKEVEMLKEFNIKDRVIIDYNCGVIEEKHKEMDKSNSHLSKEIGSTGTGCGPANVDRAMRTLRLAKEFDALKPFLGDVS
DLVNEALENGENVLIEGTQGTLLSLYYGSYPYVTSNDTSASAFASDVGIGPTKVDEVIVVFKSYPTRVGEGPFPTEMDVE
EAEKLGLVEYGTVTGRRRRIGYFDYDLAKKVCRLNGATAIALTCLDKYDEECHGLTNYDDITEKGLAFIKEIEEKVGVPV
KIISTGPELHQTIDIR
>Mature_335_residues
TCTIVVGGQWGDEGKGKIISYICEKDKPSVVARGGVGPNAGHSVEVDGKKYGIRMVPTGFPNRSSKLAVGAGVLVDVDVL
LKEVEMLKEFNIKDRVIIDYNCGVIEEKHKEMDKSNSHLSKEIGSTGTGCGPANVDRAMRTLRLAKEFDALKPFLGDVSD
LVNEALENGENVLIEGTQGTLLSLYYGSYPYVTSNDTSASAFASDVGIGPTKVDEVIVVFKSYPTRVGEGPFPTEMDVEE
AEKLGLVEYGTVTGRRRRIGYFDYDLAKKVCRLNGATAIALTCLDKYDEECHGLTNYDDITEKGLAFIKEIEEKVGVPVK
IISTGPELHQTIDIR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=28, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI34577063, Length=339, Percent_Identity=32.1533923303835, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI40316944, Length=391, Percent_Identity=27.1099744245524, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=32.311320754717, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25146553, Length=424, Percent_Identity=30.4245283018868, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25146555, Length=424, Percent_Identity=30.4245283018868, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=30.9468822170901, Blast_Score=192, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21356033, Length=332, Percent_Identity=31.0240963855422, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_META3 (A6UT04)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324238.1
- ProteinModelPortal:   A6UT04
- SMR:   A6UT04
- STRING:   A6UT04
- GeneID:   5326450
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0033
- eggNOG:   arNOG04146
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK04293
- BioCyc:   MAEO419665:MAEO_0033-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 36542; Mature: 36411

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 43-43 BINDING 127-127 BINDING 141-141 BINDING 179-179 BINDING 194-194 BINDING 256-256 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCTIVVGGQWGDEGKGKIISYICEKDKPSVVARGGVGPNAGHSVEVDGKKYGIRMVPTG
CEEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEECCCEECEEECCCC
FPNRSSKLAVGAGVLVDVDVLLKEVEMLKEFNIKDRVIIDYNCGVIEEKHKEMDKSNSHL
CCCCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
SKEIGSTGTGCGPANVDRAMRTLRLAKEFDALKPFLGDVSDLVNEALENGENVLIEGTQG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
TLLSLYYGSYPYVTSNDTSASAFASDVGIGPTKVDEVIVVFKSYPTRVGEGPFPTEMDVE
CEEEEEECCCCEEECCCCCHHHHHHHCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCHH
EAEKLGLVEYGTVTGRRRRIGYFDYDLAKKVCRLNGATAIALTCLDKYDEECHGLTNYDD
HHHHCCCEEECCCCCCCEECCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCHH
ITEKGLAFIKEIEEKVGVPVKIISTGPELHQTIDIR
HHHHHHHHHHHHHHHHCCCEEEECCCCHHHHEECCC
>Mature Secondary Structure 
TCTIVVGGQWGDEGKGKIISYICEKDKPSVVARGGVGPNAGHSVEVDGKKYGIRMVPTG
EEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEECCCEECEEECCCC
FPNRSSKLAVGAGVLVDVDVLLKEVEMLKEFNIKDRVIIDYNCGVIEEKHKEMDKSNSHL
CCCCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
SKEIGSTGTGCGPANVDRAMRTLRLAKEFDALKPFLGDVSDLVNEALENGENVLIEGTQG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
TLLSLYYGSYPYVTSNDTSASAFASDVGIGPTKVDEVIVVFKSYPTRVGEGPFPTEMDVE
CEEEEEECCCCEEECCCCCHHHHHHHCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCHH
EAEKLGLVEYGTVTGRRRRIGYFDYDLAKKVCRLNGATAIALTCLDKYDEECHGLTNYDD
HHHHCCCEEECCCCCCCEECCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCHH
ITEKGLAFIKEIEEKVGVPVKIISTGPELHQTIDIR
HHHHHHHHHHHHHHHHCCCEEEECCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA