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The map label for this gene is cbiJ [H]

Identifier: 150400210

GI number: 150400210

Start: 1495079

End: 1495828

Strand: Reverse

Name: cbiJ [H]

Synonym: Mevan_1471

Alternate gene names: 150400210

Gene position: 1495828-1495079 (Counterclockwise)

Preceding gene: 150400211

Following gene: 150400206

Centisome position: 86.96

GC content: 28.4

Gene sequence:

>750_bases
ATGAAGATATGGATTCGAGGCGGAACGAGCGATGCAGAAAAAATTTCAAAAGAAATTAAAAGAAATTTTAAAAATGTATT
TTTAATCGTTACAACAACAACAGAACTTGGCGGGGAAATTGCGAAAAGTTATGCTGATTACGTAATTTCAGAAAAAATGA
CTCGTGAAAATTTAAAAAAAATGCTTGTTGAAAACGAAATATCTATTTTTTTGGATGCAACGCACCCTTTTTCAGTGAAT
GCAAGTGAAACGGGCATAAATGTTTCCAAAGAATTAAATATTCCATATATTCGTTATGAAAGACCCGTTGAAACTTTTGA
AAACGCATATTATGTGGATAGTTTTGAAAAAGCATCTAAATTAGCGTTAAGTATTTCTAAAAAAAATATATTTTATATGG
CAGGAATAAAAAATTTAGAATCGATTTCAAAATTAATCCCTTTAGAAAGATTAATTGTTAGAATACTGCCTGTATCGATA
ATTGATGCTTTAAAAATTGTTCCTTCAAAAAATATAGTTGCAATGCAAGGCGTATTTTCTAAAGAACTAAATTATAATTT
AATAAAGGATTATAATTGTGATGTAATTATAACTAAGGATAGCGGTAAAACTGGCGGAATTTATGAAAAAGTTTCTGGTG
CACTGCTAGCAGGGGCAATCCCAATAATTGTAAACCGTCCAAAAATTGATTACCCGTTAAAATTTGAAAAAGTGGAAGAT
TTGATAAATTATTTAAAAAAATTTAATTAA

Upstream 100 bases:

>100_bases
TTCAGTATATTATACACTAATAAATAATGCTGATGGGAGTATTAGTATTGTTGAAATTTGAAAGTCATACTAAAATATTA
AAGTATTTTACGTGAAAATC

Downstream 100 bases:

>100_bases
GGTCACTGTAAGTGCTCTAAATCTTCATCATTTGGTATATATTTTATTAAATTTGGGCCAGGCCTAAGGCCATTTAGTAG
TGCCTTATTGGTATAATTTA

Product: precorrin-6x reductase

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKIWIRGGTSDAEKISKEIKRNFKNVFLIVTTTTELGGEIAKSYADYVISEKMTRENLKKMLVENEISIFLDATHPFSVN
ASETGINVSKELNIPYIRYERPVETFENAYYVDSFEKASKLALSISKKNIFYMAGIKNLESISKLIPLERLIVRILPVSI
IDALKIVPSKNIVAMQGVFSKELNYNLIKDYNCDVIITKDSGKTGGIYEKVSGALLAGAIPIIVNRPKIDYPLKFEKVED
LINYLKKFN

Sequences:

>Translated_249_residues
MKIWIRGGTSDAEKISKEIKRNFKNVFLIVTTTTELGGEIAKSYADYVISEKMTRENLKKMLVENEISIFLDATHPFSVN
ASETGINVSKELNIPYIRYERPVETFENAYYVDSFEKASKLALSISKKNIFYMAGIKNLESISKLIPLERLIVRILPVSI
IDALKIVPSKNIVAMQGVFSKELNYNLIKDYNCDVIITKDSGKTGGIYEKVSGALLAGAIPIIVNRPKIDYPLKFEKVED
LINYLKKFN
>Mature_249_residues
MKIWIRGGTSDAEKISKEIKRNFKNVFLIVTTTTELGGEIAKSYADYVISEKMTRENLKKMLVENEISIFLDATHPFSVN
ASETGINVSKELNIPYIRYERPVETFENAYYVDSFEKASKLALSISKKNIFYMAGIKNLESISKLIPLERLIVRILPVSI
IDALKIVPSKNIVAMQGVFSKELNYNLIKDYNCDVIITKDSGKTGGIYEKVSGALLAGAIPIIVNRPKIDYPLKFEKVED
LINYLKKFN

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y [H]

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003723 [H]

Pfam domain/function: PF02571 CbiJ [H]

EC number: 1.3.1.54

Molecular weight: Translated: 28173; Mature: 28173

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIWIRGGTSDAEKISKEIKRNFKNVFLIVTTTTELGGEIAKSYADYVISEKMTRENLKK
CEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVENEISIFLDATHPFSVNASETGINVSKELNIPYIRYERPVETFENAYYVDSFEKASK
HHHHCCEEEEEECCCCCCCCCCCCCCCCEEECCCCEEEECCCHHHHCCEEEECCHHHHHH
LALSISKKNIFYMAGIKNLESISKLIPLERLIVRILPVSIIDALKIVPSKNIVAMQGVFS
HEEEEECCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHH
KELNYNLIKDYNCDVIITKDSGKTGGIYEKVSGALLAGAIPIIVNRPKIDYPLKFEKVED
HCCCCCEEECCCCCEEEECCCCCCCCHHHHHCCHHHHCCEEEEEECCCCCCCCCHHHHHH
LINYLKKFN
HHHHHHHCC
>Mature Secondary Structure
MKIWIRGGTSDAEKISKEIKRNFKNVFLIVTTTTELGGEIAKSYADYVISEKMTRENLKK
CEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVENEISIFLDATHPFSVNASETGINVSKELNIPYIRYERPVETFENAYYVDSFEKASK
HHHHCCEEEEEECCCCCCCCCCCCCCCCEEECCCCEEEECCCHHHHCCEEEECCHHHHHH
LALSISKKNIFYMAGIKNLESISKLIPLERLIVRILPVSIIDALKIVPSKNIVAMQGVFS
HEEEEECCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHH
KELNYNLIKDYNCDVIITKDSGKTGGIYEKVSGALLAGAIPIIVNRPKIDYPLKFEKVED
HCCCCCEEECCCCCEEEECCCCCCCCHHHHHCCHHHHCCEEEEEECCCCCCCCCHHHHHH
LINYLKKFN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]