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The map label for this gene is moaA

Identifier: 150400184

GI number: 150400184

Start: 1469582

End: 1470475

Strand: Reverse

Name: moaA

Synonym: Mevan_1445

Alternate gene names: 150400184

Gene position: 1470475-1469582 (Counterclockwise)

Preceding gene: 150400185

Following gene: 150400183

Centisome position: 85.49

GC content: 31.88

Gene sequence:

>894_bases
ATGAAAGATGAATTTGGAAGAGAAATCAGGTCTTTTAGATTATCAATTACTCCAGAATGTAATTTAAAATGTTTCTACTG
CCATAGAGAAGGAAGAACTGAAGAAAATGGTAAATTAATGTCGCCTGATGAAATTGGTAAAATTGTTAGTGCATCCTTAG
AATTTGGAGTTCGAAAAATAAAGATTTCCGGTGGAGAGCCGCTTGTTAGAAAAGACCTTCCAAAAATTATTCAAAACATA
AAAAATGACCAGATTAAAGATATTTCACTTACTACAAATGGAATACTCCTTGAAAAGTGTGCTGAAGACCTAAAAAAGGC
AGGTCTTAATAGGGTAAACGTAAGCCTTGATACATTAAACCCTAAAAAATATAAAGAAATTACGGGCGGTGACGTTGAAA
AGGTAAAAAGAGGGATTGAAAAAGCAATTTTCTTAGGATTAACCCCTTTAAAGGTTAATTTTTTAGCAATGGACATTACA
TTAAACGACCTTTCAGAAGTAATGGACTACTGTAAAAAAGTCGGCGCAATACTCCAGATAATAGAATTCATTCCAGTAGA
TCCAAATCTTAAGCATCATCACATTGATATAACCCCTATAGAAGAAGAAATAGCAAAAAAGTCTGAAAAAGTAGTTACAA
GAAAATTCATGCAAAACCGAAAAAAATATATCTTAGACGGGCTTGAAATTGAATTTGTAAGGCCCATGGATAATACTGAA
TTTTGTGGACACTGTACAAGAATAAGATTAACTTATGATGGGTTTTTAAAGCCCTGTCTTTTAAGAGACGATAATTTGGT
TGATGTAGTAACTCCTTTACGAAATGAAGAAAGTATTCGTAGTTATTTTATAAAGTGTATTAAAAATAGAGAACCGTTTT
GTAAAGCAGAATAA

Upstream 100 bases:

>100_bases
AAAATAGTAACTTTTTTAAATTACCTGATATATAGTAGTGTTAACTTTATCATATAATTTTAATTTACATGAACATATAA
TTTAAAACGCTGTGATACTG

Downstream 100 bases:

>100_bases
AATTATTAATTTTAGTATATTTAAAGTTTTAAAAAACCATAGCTTTTTTCATTATTTTTAAAAGGCCCATAAAAAAATAG
ATAATTTTTTAAAAGTTGAA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKDEFGREIRSFRLSITPECNLKCFYCHREGRTEENGKLMSPDEIGKIVSASLEFGVRKIKISGGEPLVRKDLPKIIQNI
KNDQIKDISLTTNGILLEKCAEDLKKAGLNRVNVSLDTLNPKKYKEITGGDVEKVKRGIEKAIFLGLTPLKVNFLAMDIT
LNDLSEVMDYCKKVGAILQIIEFIPVDPNLKHHHIDITPIEEEIAKKSEKVVTRKFMQNRKKYILDGLEIEFVRPMDNTE
FCGHCTRIRLTYDGFLKPCLLRDDNLVDVVTPLRNEESIRSYFIKCIKNREPFCKAE

Sequences:

>Translated_297_residues
MKDEFGREIRSFRLSITPECNLKCFYCHREGRTEENGKLMSPDEIGKIVSASLEFGVRKIKISGGEPLVRKDLPKIIQNI
KNDQIKDISLTTNGILLEKCAEDLKKAGLNRVNVSLDTLNPKKYKEITGGDVEKVKRGIEKAIFLGLTPLKVNFLAMDIT
LNDLSEVMDYCKKVGAILQIIEFIPVDPNLKHHHIDITPIEEEIAKKSEKVVTRKFMQNRKKYILDGLEIEFVRPMDNTE
FCGHCTRIRLTYDGFLKPCLLRDDNLVDVVTPLRNEESIRSYFIKCIKNREPFCKAE
>Mature_297_residues
MKDEFGREIRSFRLSITPECNLKCFYCHREGRTEENGKLMSPDEIGKIVSASLEFGVRKIKISGGEPLVRKDLPKIIQNI
KNDQIKDISLTTNGILLEKCAEDLKKAGLNRVNVSLDTLNPKKYKEITGGDVEKVKRGIEKAIFLGLTPLKVNFLAMDIT
LNDLSEVMDYCKKVGAILQIIEFIPVDPNLKHHHIDITPIEEEIAKKSEKVVTRKFMQNRKKYILDGLEIEFVRPMDNTE
FCGHCTRIRLTYDGFLKPCLLRDDNLVDVVTPLRNEESIRSYFIKCIKNREPFCKAE

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI22027536, Length=280, Percent_Identity=33.5714285714286, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI116734668, Length=280, Percent_Identity=33.5714285714286, Blast_Score=135, Evalue=4e-32,
Organism=Escherichia coli, GI1786999, Length=305, Percent_Identity=27.8688524590164, Blast_Score=131, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32566331, Length=319, Percent_Identity=30.7210031347962, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32566329, Length=316, Percent_Identity=31.0126582278481, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17561210, Length=271, Percent_Identity=32.1033210332103, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28574490, Length=273, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28574488, Length=273, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_METVS (A6US67)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323951.1
- ProteinModelPortal:   A6US67
- SMR:   A6US67
- STRING:   A6US67
- GeneID:   5324843
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1445
- eggNOG:   arNOG05084
- HOGENOM:   HBG529885
- OMA:   IPVETDK
- ProtClustDB:   PRK00164
- HAMAP:   MF_01225_A
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013485
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02668

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 34090; Mature: 34090

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 13-13 BINDING 26-26 BINDING 61-61 BINDING 65-65 BINDING 91-91 BINDING 115-115 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEFGREIRSFRLSITPECNLKCFYCHREGRTEENGKLMSPDEIGKIVSASLEFGVRKI
CCCHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEE
KISGGEPLVRKDLPKIIQNIKNDQIKDISLTTNGILLEKCAEDLKKAGLNRVNVSLDTLN
EECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCC
PKKYKEITGGDVEKVKRGIEKAIFLGLTPLKVNFLAMDITLNDLSEVMDYCKKVGAILQI
CHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHH
IEFIPVDPNLKHHHIDITPIEEEIAKKSEKVVTRKFMQNRKKYILDGLEIEFVRPMDNTE
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEECCCCCCC
FCGHCTRIRLTYDGFLKPCLLRDDNLVDVVTPLRNEESIRSYFIKCIKNREPFCKAE
CCCCEEEEEEECCCCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKDEFGREIRSFRLSITPECNLKCFYCHREGRTEENGKLMSPDEIGKIVSASLEFGVRKI
CCCHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEE
KISGGEPLVRKDLPKIIQNIKNDQIKDISLTTNGILLEKCAEDLKKAGLNRVNVSLDTLN
EECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCC
PKKYKEITGGDVEKVKRGIEKAIFLGLTPLKVNFLAMDITLNDLSEVMDYCKKVGAILQI
CHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHH
IEFIPVDPNLKHHHIDITPIEEEIAKKSEKVVTRKFMQNRKKYILDGLEIEFVRPMDNTE
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEECCCCCCC
FCGHCTRIRLTYDGFLKPCLLRDDNLVDVVTPLRNEESIRSYFIKCIKNREPFCKAE
CCCCEEEEEEECCCCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA