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The map label for this gene is hisH [H]

Identifier: 150400011

GI number: 150400011

Start: 1297294

End: 1297905

Strand: Reverse

Name: hisH [H]

Synonym: Mevan_1269

Alternate gene names: 150400011

Gene position: 1297905-1297294 (Counterclockwise)

Preceding gene: 150400012

Following gene: 150400008

Centisome position: 75.46

GC content: 31.54

Gene sequence:

>612_bases
GTGATTGCAATAATTGACTACAATGCGGGAAACTTAAGAAGTATTGAAAAGGCTTTTGAACTTTACACTAAAGATATAAT
TGTTACAAATGATCCTGAAACCATATTTAGCGCAGATAAACTAATTTTACCAGGAGTTGGAAATTTCGGAGATTCAATGA
AAAATCTAGCTCAAAAAACTGGCGATTATTCTTTAATTGAAGTAATAAGTCGCTGCGTTGGTAAAGTACCTTTTTTAGGC
ATATGTCTTGGTATGCAACTACTTTTAGATAGGAGCGAAGAACAAAAAGAAATTTCAGGACTAAAAGTTATAAATGGGGA
CGTAATTAGGTTTAAAAATGTTCGAAAAATCCCCCATATGGGCTGGAATACTGTAAATCAAGTAAAAGACATCCCGATTT
TTGAAGGAATAAATAATAATGAATATTTTTATTTCGTACACTCCTACCATGTAAATCCAGTAGATAACAGTACAATTTCT
GGAATTACCGATTACGTTTACGAATTTCCATCAGTATTAACTAAAGGAAACATCTATGCAACACAATTTCATCCTGAAAA
AAGTGGTAAAAACGGGCTAAAAATGATAGAAAATTTCGTAGAATTGATTTAA

Upstream 100 bases:

>100_bases
TACGGGTTTAAAAGTAATTGAAGATGCATATATTGCATTTGACAAAATATCTGCAACATTAAAAGAATTAGAACAGGAAT
TATATTAAAACGGGATATTC

Downstream 100 bases:

>100_bases
TACTTATAAACAACTTAAAAACTACTAAAAAGAATTACAATTAAATAAATTTTAATTAAAATTCTATAATTAAATAACTT
CTTTTAATTTTTGTTTAGCT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIAIIDYNAGNLRSIEKAFELYTKDIIVTNDPETIFSADKLILPGVGNFGDSMKNLAQKTGDYSLIEVISRCVGKVPFLG
ICLGMQLLLDRSEEQKEISGLKVINGDVIRFKNVRKIPHMGWNTVNQVKDIPIFEGINNNEYFYFVHSYHVNPVDNSTIS
GITDYVYEFPSVLTKGNIYATQFHPEKSGKNGLKMIENFVELI

Sequences:

>Translated_203_residues
MIAIIDYNAGNLRSIEKAFELYTKDIIVTNDPETIFSADKLILPGVGNFGDSMKNLAQKTGDYSLIEVISRCVGKVPFLG
ICLGMQLLLDRSEEQKEISGLKVINGDVIRFKNVRKIPHMGWNTVNQVKDIPIFEGINNNEYFYFVHSYHVNPVDNSTIS
GITDYVYEFPSVLTKGNIYATQFHPEKSGKNGLKMIENFVELI
>Mature_203_residues
MIAIIDYNAGNLRSIEKAFELYTKDIIVTNDPETIFSADKLILPGVGNFGDSMKNLAQKTGDYSLIEVISRCVGKVPFLG
ICLGMQLLLDRSEEQKEISGLKVINGDVIRFKNVRKIPHMGWNTVNQVKDIPIFEGINNNEYFYFVHSYHVNPVDNSTIS
GITDYVYEFPSVLTKGNIYATQFHPEKSGKNGLKMIENFVELI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=39.6039603960396, Blast_Score=145, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=35.6807511737089, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22889; Mature: 22889

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYNAGNLRSIEKAFELYTKDIIVTNDPETIFSADKLILPGVGNFGDSMKNLAQKT
CEEEEECCCCCHHHHHHHHHHHHHCEEEECCCHHHHCCCCEEECCCCCCCHHHHHHHHHC
GDYSLIEVISRCVGKVPFLGICLGMQLLLDRSEEQKEISGLKVINGDVIRFKNVRKIPHM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEECCCEEEECCCCCCCCC
GWNTVNQVKDIPIFEGINNNEYFYFVHSYHVNPVDNSTISGITDYVYEFPSVLTKGNIYA
CCCCHHHHHCCCEECCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
TQFHPEKSGKNGLKMIENFVELI
EEECCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MIAIIDYNAGNLRSIEKAFELYTKDIIVTNDPETIFSADKLILPGVGNFGDSMKNLAQKT
CEEEEECCCCCHHHHHHHHHHHHHCEEEECCCHHHHCCCCEEECCCCCCCHHHHHHHHHC
GDYSLIEVISRCVGKVPFLGICLGMQLLLDRSEEQKEISGLKVINGDVIRFKNVRKIPHM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEECCCEEEECCCCCCCCC
GWNTVNQVKDIPIFEGINNNEYFYFVHSYHVNPVDNSTISGITDYVYEFPSVLTKGNIYA
CCCCHHHHHCCCEECCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
TQFHPEKSGKNGLKMIENFVELI
EEECCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA