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The map label for this gene is comB [H]

Identifier: 150399909

GI number: 150399909

Start: 1180244

End: 1180990

Strand: Reverse

Name: comB [H]

Synonym: Mevan_1166

Alternate gene names: 150399909

Gene position: 1180990-1180244 (Counterclockwise)

Preceding gene: 150399912

Following gene: 150399908

Centisome position: 68.66

GC content: 29.99

Gene sequence:

>747_bases
TTGAACGTTTCTATTTCTTATGATTTTCAATGCCAAGAAAAATATGGCTCAAGAGTTAATTTAGAAAATTCTTGTGCTGT
TGTAATAGATGTTTTACGTGCATCTACCACAATTTGTACATTAATTGAGTTGTGCAATAAGATATATATAACAGACTGTA
TCGAGAAAGCCAACAAAATCGAAAATTCTATAAAGATAGGGGAAAGAAACGGTAAAAAAATAGATGGCTTCAATTTTGGA
AACTCCCCTGTTGAATTATTATCTAATAAATTAGAAATAAAAAAATACGTGGAAAATGGCGGAAATATTGTTCTTACAAC
GACTAACGGAACACGGGTTCTTGAAAATATTTCTTCAAACTACACACTTATTGGCTCGATTACAAACGCTAAATATGTTT
CTAAAAAAGCTTGTGAAATTGCTTTTAAAAGTAATAAAGACATAACTCTTGTTCCTGCACATAGGGGTGGAAAATTTGCA
ATTGAAGATTACATATGTGCAGGATTAATTGCAGATTACATCTTAAAGTATTCTAAGGAAGAAATAGATTCAGAAAGTTT
TGAAGGACTTATACCATCAAGAACCTTAACAAAAACAGACTGGGTGAGAAAAGTATTTGAGTCAAATTCTGCTAAAAATT
TAAAAATCTTAGGCTACTATGAAGATATTTTATTTTCAGTTTCTAAAAACAGTCAAAAGTCCGTTGGAGTTTACGATAAA
AGCTTAGGAATTATAACATCTATTTAA

Upstream 100 bases:

>100_bases
TGCGAGTAGTATAATAGTTAGTATAAGAAGTACTAGAATAATTCCAAGATATTCAAAAATATTTATGTTACTTAAAACCA
TTTTATCACGGGTGAGTAAA

Downstream 100 bases:

>100_bases
TTTAGAAAATGATATATAATTAAAAAAGGCTTTTATAATTAATATTCTAACTTTTGCGGGGAATTATTTTAAACCCGCCA
TTTTACCATTTAAGTTGGAC

Product: 2-phosphosulfolactate phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNVSISYDFQCQEKYGSRVNLENSCAVVIDVLRASTTICTLIELCNKIYITDCIEKANKIENSIKIGERNGKKIDGFNFG
NSPVELLSNKLEIKKYVENGGNIVLTTTNGTRVLENISSNYTLIGSITNAKYVSKKACEIAFKSNKDITLVPAHRGGKFA
IEDYICAGLIADYILKYSKEEIDSESFEGLIPSRTLTKTDWVRKVFESNSAKNLKILGYYEDILFSVSKNSQKSVGVYDK
SLGIITSI

Sequences:

>Translated_248_residues
MNVSISYDFQCQEKYGSRVNLENSCAVVIDVLRASTTICTLIELCNKIYITDCIEKANKIENSIKIGERNGKKIDGFNFG
NSPVELLSNKLEIKKYVENGGNIVLTTTNGTRVLENISSNYTLIGSITNAKYVSKKACEIAFKSNKDITLVPAHRGGKFA
IEDYICAGLIADYILKYSKEEIDSESFEGLIPSRTLTKTDWVRKVFESNSAKNLKILGYYEDILFSVSKNSQKSVGVYDK
SLGIITSI
>Mature_248_residues
MNVSISYDFQCQEKYGSRVNLENSCAVVIDVLRASTTICTLIELCNKIYITDCIEKANKIENSIKIGERNGKKIDGFNFG
NSPVELLSNKLEIKKYVENGGNIVLTTTNGTRVLENISSNYTLIGSITNAKYVSKKACEIAFKSNKDITLVPAHRGGKFA
IEDYICAGLIADYILKYSKEEIDSESFEGLIPSRTLTKTDWVRKVFESNSAKNLKILGYYEDILFSVSKNSQKSVGVYDK
SLGIITSI

Specific function: Hydrolyzes both enantiomers of 2-phosphosulfolactate. Able to hydrolyze both enantiomers of 2-hydroxycarboxylic acids with pseudosymmetric centers of inversion. Specifically hydrolyzes (S)-phospholactate and (S)-phosphoglycerate [H]

COG id: COG2045

COG function: function code HR; Phosphosulfolactate phosphohydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ComB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005238
- InterPro:   IPR022995 [H]

Pfam domain/function: PF04029 2-ph_phosp [H]

EC number: =3.1.3.71 [H]

Molecular weight: Translated: 27582; Mature: 27582

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSISYDFQCQEKYGSRVNLENSCAVVIDVLRASTTICTLIELCNKIYITDCIEKANKI
CCEEEEECEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENSIKIGERNGKKIDGFNFGNSPVELLSNKLEIKKYVENGGNIVLTTTNGTRVLENISSN
HHHEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCC
YTLIGSITNAKYVSKKACEIAFKSNKDITLVPAHRGGKFAIEDYICAGLIADYILKYSKE
EEEEEECCCCHHHHHHHHEEEEECCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
EIDSESFEGLIPSRTLTKTDWVRKVFESNSAKNLKILGYYEDILFSVSKNSQKSVGVYDK
HCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCCCEEEC
SLGIITSI
CCCEEECC
>Mature Secondary Structure
MNVSISYDFQCQEKYGSRVNLENSCAVVIDVLRASTTICTLIELCNKIYITDCIEKANKI
CCEEEEECEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENSIKIGERNGKKIDGFNFGNSPVELLSNKLEIKKYVENGGNIVLTTTNGTRVLENISSN
HHHEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCC
YTLIGSITNAKYVSKKACEIAFKSNKDITLVPAHRGGKFAIEDYICAGLIADYILKYSKE
EEEEEECCCCHHHHHHHHEEEEECCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
EIDSESFEGLIPSRTLTKTDWVRKVFESNSAKNLKILGYYEDILFSVSKNSQKSVGVYDK
HCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCCCEEEC
SLGIITSI
CCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 11589710 [H]