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The map label for this gene is cofH

Identifier: 150399816

GI number: 150399816

Start: 1087002

End: 1088081

Strand: Reverse

Name: cofH

Synonym: Mevan_1071

Alternate gene names: 150399816

Gene position: 1088081-1087002 (Counterclockwise)

Preceding gene: 150399817

Following gene: 150399815

Centisome position: 63.26

GC content: 31.2

Gene sequence:

>1080_bases
ATGGACTTAATTTCCTTTAAAGAAAAGGATATTTCAAAAAAAGAGTGCCTAGAATTATTTTTAGATACTGAAAACTTTTT
TGATATTTTAAAAGTTGCAGATGATATTAGAAAGGAATCTGTTGGGGATACGGTTACTTACGTCTTAAATGCAAATATAA
ACTTTACAAACGTTTGCAGTGGAACGTGTAAATTTTGTGCATTTAAAACTGAAAAAGAAGACCCTAACTCATTTTTTTTA
AATCCTGATAAGGTTGCAGAAAAAGCACTTTTAGCAAGAAAAACGGGGGCTACTGAAGTATGTATTCAAGGTGGGCTCTT
ACCAGAAATTGACACATATTTTCAGGCGGAAATTTTACAAAAAGTTAAAAAAATTACTGAAAAATATGGCGGAATTGATA
TACATGCCTTTTCACCAATGGAAGTTAAATCAGCTGCTGAAAATTGTGGATTAAATGTAAAAGAAGCTTTAAAAATTTTA
AAAGATAGCGGGCTTAAAACAATGCCTGGAACCGCTGCAGAAATATTAAATGATGAAATAAGAAACGAAATATGCCCTAC
AAAACTAACCACATCAGAATGGATAGATGTTGTAAAGACGGCCCATAAAAACGGCATTAAAACAACTTGTACAATGATGT
ATGGCCATATTGAAGAAAACGTGCATTTAGTAGAACATTTATCAATTTTAAAGGAAATTCAAAAAGAAACTTCTGGATTT
ACAGAATTCGTTCCTTTAACATTTTTGCATGAAAATGCGCCTCTTCACCATATTGGAAAGGTTAAAAGCGGGGCTTCTGG
AATTTTGGATTTAAAAGTTTATGCGATTTCTAGAATATTTTTTAAAAATTACTTAAAAAACATACAGACATCATGGGTAA
AATTGGGAATTAAATTAAGCCAAATTTCACTAAATTGCGGTGCTAATGATGTTGGTGGGACACTAATGGAAGAAAGTATT
TCAAAGGCTGCCGGAGGGAGCTTTGGAACGTGTATGAGTGAAGAAAAATTGAAAAATATGATTTTAAACGTAAATAAAAT
CCCAAAAAAGAGAAATACACTTTATGAATTAATTAATTAA

Upstream 100 bases:

>100_bases
ATTATTATTAATATAATAATTATATAATGATATAATAACTAATAGAATAATAATTAAAATAAATATTTTTTTTAAATCCT
AACACTCGGCAGGTGAGCTA

Downstream 100 bases:

>100_bases
TTAATTAATTAATTAATTAATATAAAATTTCTATTTTAATGTATTGACCTAAACTTACTTATTCTGTAAAATACATACCT
CTTTTAAATGCTTGTTAAAA

Product: FO synthase subunit 2

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVLNANINFTNVCSGTCKFCAFKTEKEDPNSFFL
NPDKVAEKALLARKTGATEVCIQGGLLPEIDTYFQAEILQKVKKITEKYGGIDIHAFSPMEVKSAAENCGLNVKEALKIL
KDSGLKTMPGTAAEILNDEIRNEICPTKLTTSEWIDVVKTAHKNGIKTTCTMMYGHIEENVHLVEHLSILKEIQKETSGF
TEFVPLTFLHENAPLHHIGKVKSGASGILDLKVYAISRIFFKNYLKNIQTSWVKLGIKLSQISLNCGANDVGGTLMEESI
SKAAGGSFGTCMSEEKLKNMILNVNKIPKKRNTLYELIN

Sequences:

>Translated_359_residues
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVLNANINFTNVCSGTCKFCAFKTEKEDPNSFFL
NPDKVAEKALLARKTGATEVCIQGGLLPEIDTYFQAEILQKVKKITEKYGGIDIHAFSPMEVKSAAENCGLNVKEALKIL
KDSGLKTMPGTAAEILNDEIRNEICPTKLTTSEWIDVVKTAHKNGIKTTCTMMYGHIEENVHLVEHLSILKEIQKETSGF
TEFVPLTFLHENAPLHHIGKVKSGASGILDLKVYAISRIFFKNYLKNIQTSWVKLGIKLSQISLNCGANDVGGTLMEESI
SKAAGGSFGTCMSEEKLKNMILNVNKIPKKRNTLYELIN
>Mature_359_residues
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVLNANINFTNVCSGTCKFCAFKTEKEDPNSFFL
NPDKVAEKALLARKTGATEVCIQGGLLPEIDTYFQAEILQKVKKITEKYGGIDIHAFSPMEVKSAAENCGLNVKEALKIL
KDSGLKTMPGTAAEILNDEIRNEICPTKLTTSEWIDVVKTAHKNGIKTTCTMMYGHIEENVHLVEHLSILKEIQKETSGF
TEFVPLTFLHENAPLHHIGKVKSGASGILDLKVYAISRIFFKNYLKNIQTSWVKLGIKLSQISLNCGANDVGGTLMEESI
SKAAGGSFGTCMSEEKLKNMILNVNKIPKKRNTLYELIN

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO)

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFH_METVS (A6UR49)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323583.1
- STRING:   A6UR49
- GeneID:   5325358
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1071
- eggNOG:   arNOG05042
- HOGENOM:   HBG312134
- OMA:   TMMYGHI
- ProtClustDB:   PRK07360
- HAMAP:   MF_01612
- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004762
- SMART:   SM00729
- TIGRFAMs:   TIGR00423
- TIGRFAMs:   TIGR03551

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 39946; Mature: 39946

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVLNANINFTNVCS
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCC
GTCKFCAFKTEKEDPNSFFLNPDKVAEKALLARKTGATEVCIQGGLLPEIDTYFQAEILQ
CCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHH
KVKKITEKYGGIDIHAFSPMEVKSAAENCGLNVKEALKILKDSGLKTMPGTAAEILNDEI
HHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RNEICPTKLTTSEWIDVVKTAHKNGIKTTCTMMYGHIEENVHLVEHLSILKEIQKETSGF
HHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TEFVPLTFLHENAPLHHIGKVKSGASGILDLKVYAISRIFFKNYLKNIQTSWVKLGIKLS
HHHHHHHEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
QISLNCGANDVGGTLMEESISKAAGGSFGTCMSEEKLKNMILNVNKIPKKRNTLYELIN
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVLNANINFTNVCS
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCC
GTCKFCAFKTEKEDPNSFFLNPDKVAEKALLARKTGATEVCIQGGLLPEIDTYFQAEILQ
CCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHH
KVKKITEKYGGIDIHAFSPMEVKSAAENCGLNVKEALKILKDSGLKTMPGTAAEILNDEI
HHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RNEICPTKLTTSEWIDVVKTAHKNGIKTTCTMMYGHIEENVHLVEHLSILKEIQKETSGF
HHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TEFVPLTFLHENAPLHHIGKVKSGASGILDLKVYAISRIFFKNYLKNIQTSWVKLGIKLS
HHHHHHHEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
QISLNCGANDVGGTLMEESISKAAGGSFGTCMSEEKLKNMILNVNKIPKKRNTLYELIN
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA